LOCUS       Exported                3714 bp ds-DNA     circular SYN 25-SEP-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Laganowsky
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Sep 25, 2021 from SnapGene 5.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        77..168
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      173..182
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             179..1174
                     /codon_start=1
                     /label=Truncated Human Kir3.2 (residues 49-378)
                     /translation="MATKRKIQRYVRKDGKCNVHHGNVRETYRYLTDIFTTLVDLKWRF
                     NLLIFVMVYTVTWLFFGMIWWLIAYIRGDMDHIEDPSWTPCVTNLNGFVSAFLFSIETE
                     TTIGYGYRVITDKCPEGIILLLIQSVLGSIVNAFMVGCMFVKISQPKKRAETLVFSTHA
                     VISMRDGKLCLMFRVGDLRNSHIVEASIRAKLIKSKQTSEGEFIPLNQTDINVGYYTGD
                     DRLFLVSPLIISHEINQQSPFWEISKAQLPKEELEIVVILEGMVEATGMTCQARSSYIT
                     SEILWGYRFTPVLTLEDGFYEVDYNSFHETYETSTPSLSAKELAELASRAE"
     CDS             1187..1210
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    1237..1371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  1492..1657
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1847..2435
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  2570..2793
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2860..3393)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3582..3610)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    3681..3714
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
ORIGIN
        1 ctgtaactat aacggtccta aggtagcgag tttaaacact agtatcgatt cgcgacctac
       61 tccggaatat taatagatca tggagataat taaaatgata accatctcgc aaataaataa
      121 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaataa ccgccaccat
      181 ggccaccaaa aggaaaatcc agaggtacgt gaggaaagac ggaaagtgca atgttcatca
      241 cggcaacgtg agggagacct atcgctacct gaccgatatc ttcaccacat tagtggacct
      301 gaagtggaga ttcaacctat tgatttttgt catggtttac acagtgacct ggctcttttt
      361 tggaatgatc tggtggttga tcgcatacat acggggagac atggaccaca tagaggaccc
      421 ctcctggact ccttgtgtta ccaacctcaa cgggttcgtc tctgcttttt tattctcaat
      481 agagacagaa accaccattg gttatggcta ccgggtcatc acagataaat gcccggaggg
      541 aattattctt ctcttaatcc aatctgtgtt ggggtccatt gtcaatgcat tcatggtggg
      601 atgcatgttt gtaaaaatct ctcaacccaa gaagagggca gagaccctgg tcttttccac
      661 ccatgcagtg atctccatgc gggatgggaa actgtgcctg atgttccggg taggggacct
      721 taggaattcc cacattgtgg aggcttccat cagagccaag ttgatcaaat ccaaacagac
      781 ctcggagggg gagttcatcc cgttgaacca gacggatatc aacgtagggt attacacggg
      841 ggatgaccgt ctgtttctgg tgtcaccgct gatcattagc catgaaatta accaacagag
      901 tcctttctgg gagatctcca aagcccagct gcccaaagag gaactggaaa ttgtggtcat
      961 cctagaagga atggtggaag ccacagggat gacatgccaa gctcgaagct cctacatcac
     1021 cagtgagatc ctgtggggtt accggttcac acctgtcctg accctggagg acgggttcta
     1081 cgaagttgac tacaacagct tccatgagac ctatgagacc agcaccccat cccttagtgc
     1141 caaagagctg gccgagttag ccagcagggc agagggtgga gctagctggt cacatccaca
     1201 gtttgagaaa taataatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     1261 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1321 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg
     1381 cttgagccta gaagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     1441 caccgctgag caataactat cataacccct agggtatacc catctaattg gaaccagata
     1501 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     1561 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     1621 cacccctccc agttcccaac tattttgtcc gcccacaacc ggttgacttg ggtcaactgt
     1681 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1741 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1801 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1861 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1921 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1981 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2041 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2101 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2161 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2221 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2281 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2341 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2401 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2461 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2521 gacttgggtc gctcttcctg tggatgcgca gatgccctgc gtaagcgggt gtgggcggac
     2581 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     2641 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     2701 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     2761 gggcgtggcc aagggcatgt aaagactata ttcgcggcgt tgtgacaatt taccgaacaa
     2821 ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc
     2881 gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg
     2941 atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg
     3001 cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc
     3061 gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc
     3121 gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg
     3181 gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc
     3241 gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag
     3301 cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc
     3361 ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac atcaaacatc
     3421 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac
     3481 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
     3541 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg
     3601 cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
     3661 tgggcagcag cgaagtcgcc ataacttcgt atagcataca ttatacgaag ttat
//
