LOCUS       pEDJ8      6699 bp    DNA     circular UNA 3-OCT-2012
DEFINITION  pESC-URA from Strategene.
COMMENT     (800)357-3114 or http://www.informaxinc.com/
FEATURES             Location/Qualifiers
     CDS             417..1220
                     /vntifkey="4"
                     /label="URA3"
                     /note="URA3 from S. cerevisiae S288c"
     CDS             complement(1483..1789)
                     /vntifkey="4"
                     /label="f1 ori"
     C_region        complement(1885..2049)
                     /vntifkey="2"
                     /label="T ADH1"
     primer          2042..2065
                     /label="224"
     misc_feature    2194..2200
                     /vntifkey="21"
                     /label="Nb.BsmI"
     misc_feature    2199..2206
                     /feature_type="Acetylation site4"
                     /label="USER"
     promoter        2227..2495
                     /label="SNR52"
     seed            2496..2515
                     /label="seed_RNH1"
     gRNA            2516..2594
                     /label="gRNA scaffold"
     terminator      2595..2614
                     /label="SUP4t"
     promoter        2655..2923
                     /label="SNR52"
     seed            2924..2943
                     /label="seed_RNH201"
     gRNA            2944..3022
                     /label="gRNA scaffold"
     terminator      3023..3042
                     /label="SUP4t"
     misc_feature    3063..3072
                     /feature_type="Acetylation site4"
                     /label="USER dwn stream"
     misc_feature    3067..3073
                     /vntifkey="21"
                     /label="Nb.BsmI"
     C_region        3106..3295
                     /vntifkey="2"
                     /label="T CYC1"
     primer          complement(3111..3131)
                     /label="225"
     rep_origin      3482..4149
                     /vntifkey="33"
                     /label="pUC ori"
     CDS             complement(4300..5157)
                     /vntifkey="4"
                     /label="Amp"
     rep_origin      5291..6446
                     /vntifkey="33"
                     /label="2-micron ori"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATACCAC AGCTTTTCAA TTCAATTCAT CATTTTTTTT TTATTCTTTT TTTTGATTTC
      241 GGTTTCTTTG AAATTTTTTT GATTCGGTAA TCTCCGAACA GAAGGAAGAA CGAAGGAAGG
      301 AGCACAGACT TAGATTGGTA TATATACGCA TATGTAGTGT TGAAGAAACA TGAAATTGCC
      361 CAGTATTCTT AACCCAACTG CACAGAACAA AAACCTGCAG GAAACGAAGA TAAATCATGT
      421 CGAAAGCTAC ATATAAGGAA CGTGCTGCTA CTCATCCTAG TCCTGTTGCT GCCAAGCTAT
      481 TTAATATCAT GCACGAAAAG CAAACAAACT TGTGTGCTTC ATTGGATGTT CGTACCACCA
      541 AGGAATTACT GGAGTTAGTT GAAGCATTAG GTCCCAAAAT TTGTTTACTA AAAACACATG
      601 TGGATATCTT GACTGATTTT TCCATGGAGG GCACAGTTAA GCCGCTAAAG GCATTATCCG
      661 CCAAGTACAA TTTTTTACTC TTCGAAGACA GAAAATTTGC TGACATTGGT AATACAGTCA
      721 AATTGCAGTA CTCTGCGGGT GTATACAGAA TAGCAGAATG GGCAGACATT ACGAATGCAC
      781 ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG GTTTGAAGCA GGCGGCAGAA GAAGTAACAA
      841 AGGAACCTAG AGGCCTTTTG ATGTTAGCAG AATTGTCATG CAAGGGCTCC CTATCTACTG
      901 GAGAATATAC TAAGGGTACT GTTGACATTG CGAAGAGCGA CAAAGATTTT GTTATCGGCT
      961 TTATTGCTCA AAGAGACATG GGTGGAAGAG ATGAAGGTTA CGATTGGTTG ATTATGACAC
     1021 CCGGTGTGGG TTTAGATGAC AAGGGAGACG CATTGGGTCA ACAGTATAGA ACCGTGGATG
     1081 ATGTGGTCTC TACAGGATCT GACATTATTA TTGTTGGAAG AGGACTATTT GCAAAGGGAA
     1141 GGGATGCTAA GGTAGAGGGT GAACGTTACA GAAAAGCAGG CTGGGAAGCA TATTTGAGAA
     1201 GATGCGGCCA GCAAAACTAA AAAACTGTAT TATAAGTAAA TGCATGTATA CTAAACTCAC
     1261 AAATTAGAGC TTCAATTTAA TTATATCAGT TATTACCCTA TGCGGTGTGA AATACCGCAC
     1321 AGATGCGTAA GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT TTGTTAAAAT
     1381 TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA
     1441 TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA
     1501 AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG
     1561 GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA
     1621 AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG
     1681 CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA
     1741 GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG
     1801 GCGCGTCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC
     1861 TTCGCTATTA CGCCAGCTGA ATTGGAGCGA CCTCATGCTA TACCTGAGAA AGCAACCTGA
     1921 CCTACAGGAA AGAGTTACTC AAGAATAAGA ATTTTCGTTT TAAAACCTAA GAGTCACTTT
     1981 AAAATTTGTA TACACTTATT TTTTTTATAA CTTATTTAAT AATAAAAATC ATAAATCATA
     2041 AGAAATTCGC TTATTTAGAA GTGTCAACAA CGTATCTACC AACGATTTGA CCCTTTTCCA
     2101 TCTTTTCGTA AATTTCTGGC AAGGTAGACA AGCCGACAAC CTTGATTGGA GACTTGACCA
     2161 AACCTCTGGC GAAGAATTGT TAATTAAGAG CTCGAATGCG TGCGATaggg aacaaaagct
     2221 ggagctTCTT TGAAAAGATA ATGTATGATT ATGCTTTCAC TCATATTTAT ACAGAAACTT
     2281 GATGTTTTCT TTCGAGTATA TACAAGGTGA TTACATGTAC GTTTGAAGTA CAACTCTAGA
     2341 TTTTGTAGTG CCCTCTTGGG CTAGCGGTAA AGGTGCGCAT TTTTTCACAC CCTACAATGT
     2401 TCTGTTCAAA AGATTTTGGT CAAACGCTGT AGAAGTGAAA GTTGGTGCGC ATGTTTCGGC
     2461 GTTCGAAACT TCTCCGCAGT GAAAGATAAA TGATCGTTTG AAGACCAATT AGCTGGTTTT
     2521 AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC
     2581 CGAGTCGGTG GTGCTTTTTT TGTTTTTTAT GTCTtcgagt catgtaatta gttaagggaa
     2641 caaaagctgg agctTCTTTG AAAAGATAAT GTATGATTAT GCTTTCACTC ATATTTATAC
     2701 AGAAACTTGA TGTTTTCTTT CGAGTATATA CAAGGTGATT ACATGTACGT TTGAAGTACA
     2761 ACTCTAGATT TTGTAGTGCC CTCTTGGGCT AGCGGTAAAG GTGCGCATTT TTTCACACCC
     2821 TACAATGTTC TGTTCAAAAG ATTTTGGTCA AACGCTGTAG AAGTGAAAGT TGGTGCGCAT
     2881 GTTTCGGCGT TCGAAACTTC TCCGCAGTGA AAGATAAATG ATCGAAGCTC AGTCACGTGT
     2941 ATGGTTTTAG AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA
     3001 AGTGGCACCG AGTCGGTGGT GCTTTTTTTG TTTTTTATGT CTtcgagtca tgtaattagt
     3061 taCGCGTGCA TTCCTCGAGT AAGCTTGGTA CCGCGGCTAG CTAAGATCCG CTCTAACCGA
     3121 AAAGGAAGGA GTTAGACAAC CTGAAGTCTA GGTCCCTATT TATTTTTTTA TAGTTATGTT
     3181 AGTATTAAGA ACGTTATTTA TATTTCAAAT TTTTCTTTTT TTTCTGTACA GACGCGTGTA
     3241 CGCATGTAAC ATTATACTGA AAACCTTGCT TGAGAAGGTT TTGGGACGCT CGAAGATCCA
     3301 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
     3361 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
     3421 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
     3481 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
     3541 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     3601 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     3661 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     3721 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     3781 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     3841 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     3901 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     3961 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     4021 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     4081 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     4141 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     4201 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     4261 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     4321 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
     4381 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     4441 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     4501 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     4561 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     4621 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     4681 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     4741 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     4801 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     4861 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     4921 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     4981 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     5041 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     5101 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     5161 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     5221 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
     5281 GCCACCTGAA CGAAGCATCT GTGCTTCATT TTGTAGAACA AAAATGCAAC GCGAGAGCGC
     5341 TAATTTTTCA AACAAAGAAT CTGAGCTGCA TTTTTACAGA ACAGAAATGC AACGCGAAAG
     5401 CGCTATTTTA CCAACGAAGA ATCTGTGCTT CATTTTTGTA AAACAAAAAT GCAACGCGAG
     5461 AGCGCTAATT TTTCAAACAA AGAATCTGAG CTGCATTTTT ACAGAACAGA AATGCAACGC
     5521 GAGAGCGCTA TTTTACCAAC AAAGAATCTA TACTTCTTTT TTGTTCTACA AAAATGCATC
     5581 CCGAGAGCGC TATTTTTCTA ACAAAGCATC TTAGATTACT TTTTTTCTCC TTTGTGCGCT
     5641 CTATAATGCA GTCTCTTGAT AACTTTTTGC ACTGTAGGTC CGTTAAGGTT AGAAGAAGGC
     5701 TACTTTGGTG TCTATTTTCT CTTCCATAAA AAAAGCCTGA CTCCACTTCC CGCGTTTACT
     5761 GATTACTAGC GAAGCTGCGG GTGCATTTTT TCAAGATAAA GGCATCCCCG ATTATATTCT
     5821 ATACCGATGT GGATTGCGCA TACTTTGTGA ACAGAAAGTG ATAGCGTTGA TGATTCTTCA
     5881 TTGGTCAGAA AATTATGAAC GGTTTCTTCT ATTTTGTCTC TATATACTAC GTATAGGAAA
     5941 TGTTTACATT TTCGTATTGT TTTCGATTCA CTCTATGAAT AGTTCTTACT ACAATTTTTT
     6001 TGTCTAAAGA GTAATACTAG AGATAAACAT AAAAAATGTA GAGGTCGAGT TTAGATGCAA
     6061 GTTCAAGGAG CGAAAGGTGG ATGGGTAGGT TATATAGGGA TATAGCACAG AGATATATAG
     6121 CAAAGAGATA CTTTTGAGCA ATGTTTGTGG AAGCGGTATT CGCAATATTT TAGTAGCTCG
     6181 TTACAGTCCG GTGCGTTTTT GGTTTTTTGA AAGTGCGTCT TCAGAGCGCT TTTGGTTTTC
     6241 AAAAGCGCTC TGAAGTTCCT ATACTTTCTA GAGAATAGGA ACTTCGGAAT AGGAACTTCA
     6301 AAGCGTTTCC GAAAACGAGC GCTTCCGAAA ATGCAACGCG AGCTGCGCAC ATACAGCTCA
     6361 CTGTTCACGT CGCACCTATA TCTGCGTGTT GCCTGTATAT ATATATACAT GAGAAGAACG
     6421 GCATAGTGCG TGTTTATGCT TAAATGCGTA CTTATATGCG TCTATTTATG TAGGATGAAA
     6481 GGTAGTCTAG TACCTCCTGT GATATTATCC CATTCCATGC GGGGTATCGT ATGCTTCCTT
     6541 CAGCACTACC CTTTAGCTGT TCTATATGCT GCCACTCCTC AATTGGATTA GTCTCATCCT
     6601 TCAATGCTAT CATTTCCTTT GATATTGGAT CATACTAAGA AACCATTATT ATCATGACAT
     6661 TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTC
//
