LOCUS       pMLB10      8962 bp    DNA     circular UNA 27-JAN-2012
DEFINITION  pESC-TRP from Strategene.
COMMENT     (800)357-3114 or http://www.informaxinc.com/
FEATURES             Location/Qualifiers
     CDS             468..1139
                     /vntifkey="4"
                     /label="TRP1"
     CDS             complement(1373..1679)
                     /vntifkey="4"
                     /label="f1 ori"
     terminator      1779..1943
                     /vntifkey="43"
                     /label="T ADH1"
     misc_feature    1936..1959
                     /feature_type="1' clip"
                     /label="Primer 224"
     misc_feature    2088..2094
                     /vntifkey="21"
                     /label="Nb.BsmI"
     misc_feature    2090..2100
                     /feature_type="1' clip"
                     /label="User up"
     misc_feature    2101..2636
                     /feature_type="Acetylations iste7"
                     /label="GAL1p"
     misc_feature    2655..2675
                     /feature_type="Acetylations iste6"
                     /label="NLS"
     misc_feature    2676..5324
                     /feature_type="Acetylations iste8"
                     /label="T7RNAP(F11L, T613A)"
     Gene13.01       2703..2705
                     /label="F11->L"
     Gene13.01       4509..4511
                     /label="T613->A"
     misc_feature    5325..5336
                     /feature_type="1' clip"
                     /label="User down"
     misc_feature    complement(5325..5336)
                     /feature_type="4' UTR"
                     /label="DOWNSTREAM user site"
     misc_feature    5331..5336
                     /vntifkey="21"
                     /label="Nb.BsmI"
     terminator      5369..5558
                     /vntifkey="43"
                     /label="T CYC1"
     misc_feature    complement(5374..5394)
                     /feature_type="1' clip"
                     /label="Primer 225"
     rep_origin      5745..6412
                     /vntifkey="33"
                     /label="pUC ori"
     CDS             complement(6653..7420)
                     /vntifkey="4"
                     /label="Amp"
     rep_origin      7554..8709
                     /vntifkey="33"
                     /label="2-micron ori"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATAAACG ACATTACTAT ATATATAATA TAGGAAGCAT TTAATAGACA GCATCGTAAT
      241 ATATGTGTAC TTTGCAGTTA TGACGCCAGA TGGCAGTAGT GGAAGATATT CTTTATTGAA
      301 AAATAGCTTG TCACCTTACG TACAATCTTG ATCCGGAGCT TTTCTTTTTT TGCCGATTAA
      361 GAATTAATTC GGTCGAAAAA AGAAAAGGAG AGGGCCAAGA GGGAGGGCAT TGGTGACTAT
      421 TGAGCACGTG AGTATACGTG ATTAAGCACA CAAAGGCAGC TTGGAGTATG TCTGTTATTA
      481 ATTTCACAGG TAGTTCTGGT CCATTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG
      541 CAGAATGTGC TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA
      601 AGAGAACAAT TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA
      661 ATAGTTCAGG CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG
      721 TTTTGGCTCT GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT
      781 GGCAAGAATA CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG
      841 ACTGCAACAT ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG
      901 ATTCAGAAGC AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG
      961 GAAGGCAAGA GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA
     1021 ATGTTGGTGA TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG
     1081 AGACAAATGG TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT
     1141 AGGTTATTAC TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCTA TGCGGTGTGA
     1201 AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT
     1261 TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA
     1321 ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA
     1381 GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC
     1441 GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG
     1501 AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG
     1561 GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG
     1621 GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG
     1681 CCGCTACAGG GCGCGTCGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA
     1741 TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGAATTGGA GCGACCTCAT GCTATACCTG
     1801 AGAAAGCAAC CTGACCTACA GGAAAGAGTT ACTCAAGAAT AAGAATTTTC GTTTTAAAAC
     1861 CTAAGAGTCA CTTTAAAATT TGTATACACT TATTTTTTTT ATAACTTATT TAATAATAAA
     1921 AATCATAAAT CATAAGAAAT TCGCTTATTT AGAAGTGTCA ACAACGTATC TACCAACGAT
     1981 TTGACCCTTT TCCATCTTTT CGTAAATTTC TGGCAAGGTA GACAAGCCGA CAACCTTGAT
     2041 TGGAGACTTG ACCAAACCTC TGGCGAAGAA TTGTTAATTA AGAGCTCGAA TGCGTGCGAT
     2101 GAGCCCCATT ATCTTAGCCT AAAAAAACCT TCTCTTTGGA ACTTTCAGTA ATACGCTTAA
     2161 CTGCTCATTG CTATATTGAA GTACGGATTA GAAGCCGCCG AGCGGGCGAC AGCCCTCCGA
     2221 CGGAAGACTC TCCTCCGTGC GTCCTGGTCT TCACCGGTCG CGTTCCTGAA ACGCAGATGT
     2281 GCCTCGCGCC GCACTGCTCC GAACAATAAA GATTCTACAA TACTAGCTTT TATGGTTATG
     2341 AAGAGGAAAA ATTGGCAGTA ACCTGGCCCC ACAAACCTTC AAATCAACGA ATCAAATTAA
     2401 CAACCATAGG ATAATAATGC GATTAGTTTT TTAGCCTTAT TTCTGGGGTA ATTAATCAGC
     2461 GAAGCGATGA TTTTTGATCT ATTAACAGAT ATATAAATGC AAAAGCTGCA TAACCACTTT
     2521 AACTAATACT TTCAACATTT TCGGTTTGTA TTACTTCTTA TTCAAATGTC ATAAAAGTAT
     2581 CAACAAAAAA TTGTTAATAT ACCTCTATAC TTTAACGTCA AGGAGAAAAA ACTATAATCT
     2641 GTCATAAAAC AatgCCCAAG AAGAAGAGGA AGGTGaacac gattaacatc gctaagaacg
     2701 acttGtctga catcgaactg gctgctatcc cgttcaacac tctggctgac cattacggtg
     2761 agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg ggtgaagcac
     2821 gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat aacgctgccg
     2881 ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac gactggtttg
     2941 aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg caagaaatca
     3001 agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta accagtgctg
     3061 acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag gacgaggctc
     3121 gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac
     3181 tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc gaggctgaca
     3241 tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag gaagactcta
     3301 ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg gttagcttac
     3361 accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc gcacctgaat
     3421 acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg atgttccaac
     3481 cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat tgggctaacg
     3541 gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg cgctacgaag
     3601 acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa
     3661 tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg
     3721 aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac atcgacatga
     3781 atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg
     3841 ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat aagtttgcta
     3901 accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt
     3961 caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg gcgaaaggta
     4021 aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg
     4081 tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac gagaacatca
     4141 tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat tctccgttct
     4201 gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg agctataact
     4261 gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc tccgcgatgc
     4321 tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca
     4381 tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga
     4441 ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca
     4501 agctgggcGc taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga
     4561 ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag
     4621 tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc
     4681 cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg
     4741 tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg
     4801 tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc
     4861 ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttgaacctga
     4921 tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat agcgagattg
     4981 atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa gacggtagcc
     5041 accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct tttgcactga
     5101 ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg
     5161 aaactatggt tgacacatat gagtcttgtg atgtactggc tgatttctac gaccagttcg
     5221 ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct aaaggtaact
     5281 tgaacctccg tgacatctta gagtcggact tcgcgttcgc gtaaATCGCG TGCATCCTCG
     5341 AGTAAGCTTG GTACCGCGGC TAGCTAAGAT CCGCTCTAAC CGAAAAGGAA GGAGTTAGAC
     5401 AACCTGAAGT CTAGGTCCCT ATTTATTTTT TTATAGTTAT GTTAGTATTA AGAACGTTAT
     5461 TTATATTTCA AATTTTTCTT TTTTTTCTGT ACAGACGCGT GTACGCATGT AACATTATAC
     5521 TGAAAACCTT GCTTGAGAAG GTTTTGGGAC GCTCGAAGAT CCAGCTGCAT TAATGAATCG
     5581 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG
     5641 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA
     5701 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC
     5761 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC
     5821 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
     5881 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
     5941 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT
     6001 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG
     6061 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC
     6121 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA
     6181 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA
     6241 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
     6301 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC
     6361 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG
     6421 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA
     6481 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG
     6541 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT
     6601 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG
     6661 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC
     6721 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA
     6781 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC
     6841 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT
     6901 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC
     6961 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT
     7021 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC
     7081 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT
     7141 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA
     7201 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA
     7261 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG
     7321 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA
     7381 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT
     7441 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA
     7501 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAACGAAGCA
     7561 TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT TCAAACAAAG
     7621 AATCTGAGCT GCATTTTTAC AGAACAGAAA TGCAACGCGA AAGCGCTATT TTACCAACGA
     7681 AGAATCTGTG CTTCATTTTT GTAAAACAAA AATGCAACGC GAGAGCGCTA ATTTTTCAAA
     7741 CAAAGAATCT GAGCTGCATT TTTACAGAAC AGAAATGCAA CGCGAGAGCG CTATTTTACC
     7801 AACAAAGAAT CTATACTTCT TTTTTGTTCT ACAAAAATGC ATCCCGAGAG CGCTATTTTT
     7861 CTAACAAAGC ATCTTAGATT ACTTTTTTTC TCCTTTGTGC GCTCTATAAT GCAGTCTCTT
     7921 GATAACTTTT TGCACTGTAG GTCCGTTAAG GTTAGAAGAA GGCTACTTTG GTGTCTATTT
     7981 TCTCTTCCAT AAAAAAAGCC TGACTCCACT TCCCGCGTTT ACTGATTACT AGCGAAGCTG
     8041 CGGGTGCATT TTTTCAAGAT AAAGGCATCC CCGATTATAT TCTATACCGA TGTGGATTGC
     8101 GCATACTTTG TGAACAGAAA GTGATAGCGT TGATGATTCT TCATTGGTCA GAAAATTATG
     8161 AACGGTTTCT TCTATTTTGT CTCTATATAC TACGTATAGG AAATGTTTAC ATTTTCGTAT
     8221 TGTTTTCGAT TCACTCTATG AATAGTTCTT ACTACAATTT TTTTGTCTAA AGAGTAATAC
     8281 TAGAGATAAA CATAAAAAAT GTAGAGGTCG AGTTTAGATG CAAGTTCAAG GAGCGAAAGG
     8341 TGGATGGGTA GGTTATATAG GGATATAGCA CAGAGATATA TAGCAAAGAG ATACTTTTGA
     8401 GCAATGTTTG TGGAAGCGGT ATTCGCAATA TTTTAGTAGC TCGTTACAGT CCGGTGCGTT
     8461 TTTGGTTTTT TGAAAGTGCG TCTTCAGAGC GCTTTTGGTT TTCAAAAGCG CTCTGAAGTT
     8521 CCTATACTTT CTAGAGAATA GGAACTTCGG AATAGGAACT TCAAAGCGTT TCCGAAAACG
     8581 AGCGCTTCCG AAAATGCAAC GCGAGCTGCG CACATACAGC TCACTGTTCA CGTCGCACCT
     8641 ATATCTGCGT GTTGCCTGTA TATATATATA CATGAGAAGA ACGGCATAGT GCGTGTTTAT
     8701 GCTTAAATGC GTACTTATAT GCGTCTATTT ATGTAGGATG AAAGGTAGTC TAGTACCTCC
     8761 TGTGATATTA TCCCATTCCA TGCGGGGTAT CGTATGCTTC CTTCAGCACT ACCCTTTAGC
     8821 TGTTCTATAT GCTGCCACTC CTCAATTGGA TTAGTCTCAT CCTTCAATGC TATCATTTCC
     8881 TTTGATATTG GATCATATTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG
     8941 CGTATCACGA GGCCCTTTCG TC
//
