LOCUS       pEDJ506      6455 bp    DNA     circular UNA 2-DEC-2014
DEFINITION  PCR product of p2073 (pXII-5_no_BsaI).
KEYWORDS    PCR_PRODUCT.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     primer          1..25
                     /vntifkey="27"
                     /label="SENSE_PRM"
                     /note="Sense primer used for creating BB0612
                     (XII-5_w/o_marker)"
     misc_feature    117..792
                     /vntifkey="21"
                     /label="XII-5 Down"
     Site            join(850..855,850^851)
                     /site_type="restriction site"
                     /note="Name: XbaI"
                     /note="Pattern: tctaga"
                     /note="Number_of_matches: 3"
                     /note="cut_0_on_positive_strand: 850^851"
                     /note="cut_0_on_negative_strand: 854^855"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label="XbaI"
     Site            join(850..855,850^851)
                     /site_type="restriction site"
                     /note="Name: XbaI"
                     /note="Pattern: tctaga"
                     /note="Number_of_matches: 3"
                     /note="cut_0_on_positive_strand: 850^851"
                     /note="cut_0_on_negative_strand: 854^855"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label="XbaI"
     rep_origin      1569..2234
                     /vntifkey="33"
                     /label="pUC ori"
     CDS             complement(2336..3196)
                     /vntifkey="4"
                     /label="AmpR"
     Site            join(3594..3599,3594^3595)
                     /site_type="restriction site"
                     /note="Name: XbaI"
                     /note="Pattern: tctaga"
                     /note="Number_of_matches: 3"
                     /note="cut_0_on_positive_strand: 3594^3595"
                     /note="cut_0_on_negative_strand: 3598^3599"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label="XbaI"
     Site            join(3594..3599,3594^3595)
                     /site_type="restriction site"
                     /note="Name: XbaI"
                     /note="Pattern: tctaga"
                     /note="Number_of_matches: 3"
                     /note="cut_0_on_positive_strand: 3594^3595"
                     /note="cut_0_on_negative_strand: 3598^3599"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label="XbaI"
     misc_feature    3655..4108
                     /vntifkey="21"
                     /label="XII-5 UP"
     misc_feature    4148..4346
                     /feature_type="Acetylation site50"
                     /label="ADH1t forward"
     terminator      4149..4310
                     /vntifkey="43"
                     /label="T ADH1"
     misc_feature    complement(4360..4579)
                     /feature_type="Acetylation site4"
                     /label="mADH1t"
     misc_feature    complement(4590..5252)
                     /feature_type="Acetylation site9"
                     /label="mhis3_AI"
     misc_feature    complement(5016..5038)
                     /feature_type="10' clip"
                     /label="PAM+gRNA site"
     gene2           5019..5023
                     /label="5 nt inversion"
     misc_feature    5253..5261
                     /feature_type="Acetylations iste0"
                     /label="USER overhang"
     misc_feature    complement(5262..6247)
                     /feature_type="Acetylation site1"
                     /label="pPGK1"
     terminator      6259..6447
                     /vntifkey="43"
                     /label="T CYC1"
     primer          complement(6430..6455)
                     /vntifkey="27"
                     /label="ANTISENSE_PRM"
                     /note="Antisense primer used for creating BB0612
                     (XII-5_w/o_marker)"
ORIGIN
        1 ACCCAATTCG CCCTATAGTG AGTCGTATTA CGCGCGCTCA CTGGCCGTCG TTTTACAACG
       61 TCGTGACTGG GAAAACCCTG GCGTTACCCC TGCAGGACTA GTGCTGAGGC ATTAATGCAA
      121 CTCAGAAGTT TGACAGCAAG CAAGTTCATC ATTCGAACTA GCCTTATTGT TTTAGTTCAG
      181 TGACAGCGAA CTGCCGTACT CGATGCTTTA TTTCTCACGG TAGAGCGGAA GAACAGATAG
      241 GGGCAGCGTG AGAAGAGTTA GAAAGTAAAT TTTTATCACG TCTGAAGTAT TCTTATTCAT
      301 AGGAAATTTT GCAAGGTTTT TTAGCTCAAT AACGGGCTAA GTTATATAAG GTGTTCACGC
      361 GATTTTCTTG TTATGTATAC CTCTTCTCTG AGGAATGGTA CTACTGTCCT GATGTAGGCT
      421 CCTTAAATTG GTGGGCAAGA ATAACTTATC GATATTTTGT ATATTGGTCT TGGAGTTCAC
      481 CACGTAATGC CTGTTTAAGA CCATCAGTTA ACTCTAGTAT TATTTGGTCT TGGCTACTGG
      541 CCGTTTGCTA TTATTCAAGT CTTTTGTGCC TTCCCGTCGG GTAAGGGAGT TATTTAGGGA
      601 TACAGAATCT AACGAAAACT AAATCTCAAT GATTAACTCT ATTTAATCCT TTTTTGAAAG
      661 GCAAAAGAGG TCCCTTGTTC ACTTACAACG TTCTTAGCCA AATTCGCTTA TCACTTACTA
      721 CTTCACGATA TACAGAAGTA AAAACATATA AAAAGATGTC TGTTTGTTTA GCCATCACAA
      781 AAGGTATCGC AGATTAAGAC CTCAGCGCGG CCGCAAATTT AAATAAAATG AAGTGAAGTT
      841 CCTATACTTT CTAGAGAATA GGAACTTCTA TAGTGAGTCG AATAAGGGCG ACACAAAATT
      901 TATTCTAAAT GCATAATAAA TACTGATAAC ATCTTATAGT TTGTATTATA TTTTGTATTA
      961 TCGTTGACAT GTATAATTTT GATATCAAAA ACTGATTTTC CCTTTATTAT TTTCGAGATT
     1021 TATTTTCTTA ATTCTCTTTA ACAAACTAGA AATATTGTAT ATACAAAAAA TCATAAATAA
     1081 TAGATGAATA GTTTAATTAT AGGTGTTCAT CAATCGAAAA AGCAACGTAT CTTATTTAAA
     1141 GTGCGTTGCT TTTTTCTCAT TTATAAGGTT AAATAATTCT CATATATCAA GCAAAGTGAC
     1201 AGGCGCCCTT AAATATTCTG ACAAATGCTC TTTCCCTAAA CTCCCCCCAT AAAAAAACCC
     1261 GCCGAAGCGG GTTTTTACGT TATTTGCGGA TTAACGATTA CTCGTTATCA GAACCGCCCA
     1321 GGGGGCCCGA GCTTAAGACT GGCCGTCGTT TTACAACACA GAAAGAGTTT GTAGAAACGC
     1381 AAAAAGGCCA TCCGTCAGGG GCCTTCTGCT TAGTTTGATG CCTGGCAGTT CCCTACTCTC
     1441 GCCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
     1501 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
     1561 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
     1621 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1681 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1741 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1801 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1861 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1921 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1981 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     2041 GGCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     2101 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     2161 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
     2221 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAC GCGCGCGTAA CTCACGTTAA
     2281 GGGATTTTGG TCATGAGCTT GCGCCGTCCC GTCAAGTCAG CGTAATGCTC TGCTTTTACC
     2341 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
     2401 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGCG
     2461 CTGCGATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCGGA TTTATCAGCA ATAAACCAGC
     2521 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
     2581 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
     2641 TTGCCATCGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
     2701 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
     2761 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
     2821 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
     2881 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
     2941 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
     3001 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
     3061 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
     3121 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
     3181 AATGTTGAAT ACTCATATTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT
     3241 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTCAGTG
     3301 TTACAACCAA TTAACCAATT CTGAACATTA TCGCGAGCCC ATTTATACCT GAATATGGCT
     3361 CATAACACCC CTTGTTTGCC TGGCGGCAGT AGCGCGGTGG TCCCACCTGA CCCCATGCCG
     3421 AACTCAGAAG TGAAACGCCG TAGCGCCGAT GGTAGTGTGG GGACTCCCCA TGCGAGAGTA
     3481 GGGAACTGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGCCC
     3541 GGGCTAATTA TGGGGTGTCG CCCTTATTCG ACTCTATAGT GAAGTTCCTA TTCTCTAGAA
     3601 AGTATAGGAA CTTCTGAAGT GGGGATTTAA ATGCGGCCGC GCTGAGGGTT TAATCAATCT
     3661 GGCGGCTTGA GTTCTCAACA TGTTTTATTT TTTACTTATA TTGCTGGTAG GGTAAAAAAA
     3721 TATAACTCCT AGGAATAGGT TGTCTATATG TTTTTGTCTT GCTTCTATAA TTGTAACAAA
     3781 CAAGGAAAGG GAAAATACTG GGTGTAAAAG CCATTGAGTC AAGTTAGGTC ATCCCTTTTA
     3841 TACAAAATTT TTCAATTTTT TTTCCAAGAT TCTTGTACGA TTAATTATTT TTTTTTTGCG
     3901 TCCTACAGCG TGATGAAAAT TTCGCCTGCT GCAAGATGAG CGGGAACGGG CGAAATGTGC
     3961 ACGCGCACAA CTTACGAAAC GCGGATGAGT CACTGACAGC CACCGCAGAG GTTCTGACTC
     4021 CTACTGAGCT CTATTGGAGG TGGCAGAACC GGTACCGGAG GAGGCCGCTA TAACCGGTTT
     4081 GAATTTATTG TCACAGTGTC ACATCAGCAT TAAGTCCTCA GCGAGCTCGC ATGGAATGCG
     4141 TGCGATGAGC GACCTCATGC TATACCTGAG AAAGCAACCT GACCTACAGG AAAGAGTTAC
     4201 TCAAGAATAA GAATTTTCGT TTTAAAACCT AAGAGTCACT TTAAAATTTG TATACACTTA
     4261 TTTTTTTTAT AACTTATTTA ATAATAAAAA TCATAAATCA TAAGAAATTC GCTTATTTAG
     4321 AAGTGTCAAC AACGTATCTA CCAACGGAAT GCGTGCGATC GTTGGTAGAT ACGTTGTTGA
     4381 CACTTCTAAA TAAGCGAATT TCTTATGATT TATGATTTTT ATTATTAAAT AAGTTATAAA
     4441 AAAAATAAGT GTATACAAAT TTTAAAGTGA CTCTTAGGTT TTAAAACGAA AATTCTTATT
     4501 CTTGAGTAAC TCTTTCCTGT AGGTCAGGTT GCTTTCTCAG GTATAGCATG AGGTCGCTCG
     4561 TTAATTAAGA GCTCGAATGC ATCTGTCATC TACATAAGAA CACCTTTGGT GGAGGGAACA
     4621 TCGTTGGTAC CATTGGGCGA GGTGGCTTCT CTTATGGCAA CCGCAAGAGC CTTGAACGCA
     4681 CTCTCACTAC GGTGATGATC ATTCTTGCCT CGCAGACAAT CAACGTGGAG GGTAATTCTG
     4741 CTAGCCTCTG CAAAGCTTTC AAGAAAATGC GGGATCATCT CGCAAGAGAG ATCTCCTACT
     4801 TTCTCCCTTT GCAAACCAAG TTCGACAACT GCGTACGGCC TGTTCGAAAG ATCTACCACC
     4861 GCTCTGGAAA GTGCCTCATC CAAAGGCGCA AATCCTGATC CAAACCTTTT TACTCCACGC
     4921 ACGGCCCCTA GGGCCTCTTT AAAAGCTTGA CCGAGAGCAA TCCCGCAGTC TTCAGTGGTG
     4981 TGATGGTCGT CTATGTGTAA GTCACCAATG CACTCCCGTC GAGGAATTTT TACTAACACC
     5041 AGAGCATGTA TCATATGGTC CAGAAACCCT ATACCTGTGT GGACGTTAAT CACTTGCGAT
     5101 TGTGTGGCCT GTTCTGCTAC TGCTTCTGCC TCTTTTTCTG GGAAGATCGA GTGCTCTATC
     5161 GCTAGGGGAC CACCCTTTAA AGAGATCGCA ATCTGAATCT TGGTTTCATT TGTAATACGC
     5221 TTTACTAGGG CTTTCTGCTC TGTCATTGTT TTAGTGCAGG TTGTTTTATA TTTGTTGTAA
     5281 AAAGTAGATA ATTACTTCCT TGATGATCTG TAAAAAAGAG AAAAAGAAAG CATCTAAGAA
     5341 CTTGAAAAAC TACGAATTAG AAAAGACCAA ATATGTATTT CTTGCATTGA CCAATTTATG
     5401 CAAGTTTATA TATATGTAAA TGTAAGTTTC ACGAGGTTCT ACTAAACTAA ACCACCCCCT
     5461 TGGTTAGAAG AAAAGAGTGT GTGAGAACAG GCTGTTGTTG TCACACGATT CGGACAATTC
     5521 TGTTTGAAAG AGAGAGAGTA ACAGTACGAT CGAACGAACT TTGCTCTGGA GATCACAGTG
     5581 GGCATCATAG CATGTGGTAC TAAACCCTTT CCCGCCATTC CAGAACCTTC GATTGCTTGT
     5641 TACAAAACCT GTGAGCCGTC GCTAGGACCT TGTTGTGTGA CGAAATTGGA AGCTGCAATC
     5701 AATAGGAAGA CAGGAAGTCG AGCGTGTCTG GGTTTTTTCA GTTTTGTTCT TTTTGCAAAC
     5761 AAATCACGAG CGACGGTAAT TTCTTTCTCG ATAAGAGGCC ACGTGCTTTA TGAGGGTAAC
     5821 ATCAATTCAA GAAGGAGGGA AACACTTCCT TTTTCTGGCC CTGATAATAG TATGAGGGTG
     5881 AAGCCAAAAT AAAGGATTCG CGCCCAAATC GGCATCTTTA AATGCAGGTA TGCGATAGTT
     5941 CCTCACTCTT TCCTTACTCA CGAGTAATTC TTGCAAATGC CTATTATGCA GATGTTATAA
     6001 TATCTGTGCG TCTTGAGTTG AAGTCAGGAA TCTAAAATAA AAATTAAGGT TAATAAAAAG
     6061 AGGAAAGAAA AAAAAATTAA TCGATTTACA GAAACTTGCA CACTAAAAAT ACACAACTAA
     6121 AAGCAATTAC AGTATGGGAA GTCATCGACG TTATCTCTAC TATAGTATAT TATCATTTCT
     6181 ATTATTATCC TGCTCAGTGG TACTTGCAAA ACAAGATAAG ACCCCATTCT TTGAAGGTAC
     6241 TTCTTCGCGC GTGCATTCAT CCGCTCTAAC CGAAAAGGAA GGAGTTAGAC AACCTGAAGT
     6301 CTAGGTCCCT ATTTATTTTT TTATAGTTAT GTTAGTATTA AGAACGTTAT TTATATTTCA
     6361 AATTTTTCTT TTTTTTCTGT ACAGACGCGT GTACGCATGT AACATTATAC TGAAAACCTT
     6421 GCTTGAGAAG GTTTTGGGAC GCTCGAAGAT CGCGT
//
