LOCUS       pYTRW10K_0x5            9699 bp    DNA     circular SYN 27-SEP-2021
DEFINITION  
ACCESSION   pYTRW10K_0x5
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     promoter        104..155
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /label=P mob
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P mob"
                     /SECDescr="mob promoter including oriT (Szpirer et al 2001, PMID: 11222611)."
     CDS             223..1242
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID:
                     19928068328)."
                     /transl_table=11
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mob"
                     /SECDescr="Mob protein necessary for mobilization"
     misc_feature    1243..1261
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-L
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-L"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     misc_feature    1262..1301
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /label=Core_up
                     /SECDrawAs="Label"
                     /SECName="Core_up"
                     /SECDescr="upstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    1302..1316
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    1317..1356
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /SECDrawAs="Label"
                     /SECName="P_up"
                     /SECDescr="upstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1357..1393
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /label=PI-SceI
                     /SECDrawAs="Label"
                     /SECName="PI-SceI"
                     /SECDescr="recognition site of homing endonuclease PI-SceI, enabling e.g. promoter insertion."
     misc_feature    1394..1401
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /label=AsiSI
                     /SECDrawAs="Label"
                     /SECName="AsiSI"
                     /SECDescr="recognition site of endonuclease AsiSI, enabling e.g. promoter insertion."
     misc_feature    1402..1441
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /label=P_dn
                     /SECDrawAs="Label"
                     /SECName="P_dn"
                     /SECDescr="downstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1402..1521
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_up
                     /SECDrawAs="Label"
                     /SECName="CIS_up"
                     /SECDescr="upstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1522..1539
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /label=I-SceI
                     /SECDrawAs="Label"
                     /SECName="I-SceI"
                     /SECDescr="recognition site of homing endonuclease I-SceI, enabling target gene insertion."
     misc_feature    1540..1659
                     /note="downstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_dn
                     /SECDrawAs="Label"
                     /SECName="CIS_dn"
                     /SECDescr="downstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1620..1659
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /gene="R_up"
                     /SECDrawAs="Label"
                     /SECName="R_up"
                     /SECDescr="upstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    1660..1667
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion."
                     /label=MauBI
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion."
     misc_feature    1668..1693
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion."
                     /label=I-CeuI
                     /SECDrawAs="Label"
                     /SECName="I-CeuI"
                     /SECDescr="recognition site of homing endonuclease I-CeuI, enabling e.g. reporter insertion."
     misc_feature    1694..1733
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /label=R_dn
                     /SECDrawAs="Label"
                     /SECName="R_dn"
                     /SECDescr="downstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    1733..1790
                     /label=MCS Integration site
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /SECDrawAs="Info only"
     misc_feature    1734..1789
                     /note="region with Multiple Cutters
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon
                     Tn5 insertion site elucidation."
                     /label=MC
                     /SECDrawAs="Label"
                     /SECName="MC"
                     /SECDescr="region with Multiple Cutters EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon Tn5 insertion site elucidation."
     misc_feature    1790..1829
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_up
                     /SECDrawAs="Label"
                     /SECName="M_up"
                     /SECDescr="upstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    1830..1835
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /label=SalI
                     /SECDrawAs="Label"
                     /SECName="SalI"
                     /SECDescr="recognition site of endonuclease SalI, enabling e.g. marker insertion."
     misc_feature    1836..1865
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /label=PI-PspI
                     /SECDrawAs="Label"
                     /SECName="PI-PspI"
                     /SECDescr="recognition site of homing endonuclease PI-PspI, enabling e.g. marker insertion."
     misc_feature    1866..1905
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_dn
                     /SECDrawAs="Label"
                     /SECName="M_dn"
                     /SECDescr="downstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    1906..1920
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    1921..1960
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /label=Core_dn
                     /SECDrawAs="Label"
                     /SECName="Core_dn"
                     /SECDescr="downstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    complement(1961..1979)
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-R
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-R"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     promoter        1990..2020
                     /note="transposon Tn5 transposase promoter (Yin et al
                     1988, PMID: 2451025)."
                     /label=P tnp
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tnp"
                     /SECDescr="transposon Tn5 transposase promoter (Yin et al 1988, PMID: 2451025)."
     CDS             2053..3483
                     /gene="tnp"
                     /product="transposon Tn5 transposase"
                     /transl_table=11
                     /note="transposon Tn5 transposase (Goryshin & Reznikoff
                     1998, PMID: 9516433)."
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGK
                     SITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLS
                     YRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEK
                     ESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDV
                     ESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGN
                     ITLNAVLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKT
                     GAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQS
                     AETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW
                     EGWEALQSKLDGFLAAKDLMAQGIKI"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tnp"
                     /SECDescr="transposon Tn5 transposase"
     misc_feature    3995..4105
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /label=CEN4
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="CEN4"
                     /SECDescr="CEN4 of Saccharomyces cerevisiae Chr IV (Saccharomyces Genome Database)."
     misc_feature    4637..5381
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /label=ARS1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ARS1 "
                     /SECDescr="ARS1 of yCP50 (Genbank: X70276.1)."
     rep_origin      5851..6439
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387);
                     annotated as New England Biolabs (2007)."
                     /label=pMB1 ori
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pMB1 ori"
                     /SECDescr="origin of replication from plasmid pBR322 (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); annotated as New England Biolabs (2007)."
     CDS             complement(6618..7412)
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /transl_table=11
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aphII"
                     /SECDescr="aminoglycoside 3'-phosphotransferase"
     promoter        7502..7550
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank
                     U00004.1)."
                     /label=P aphII
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aphII"
                     /SECDescr="promoter of aphII gene in Transposon Tn5 (Genbank U00004.1)."
     promoter        7850..8065
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /label=P URA3
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P URA3"
                     /SECDescr="URA3 promoter region (Roy et al 1990, PMID: 2204810)."
     CDS             8066..8869
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="URA3"
                     /SECDescr="orotidine 5'-phosphate decarboxylase"
ORIGIN      
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgtaa ctataacggt
     1681 cctaaggtag cgagcttact tgtgctgttg tgtaaggaca cgcttaaccc agagaattcc
     1741 agcgacctat tctacgacta cgcgtccatg gctcgaggag ctcggtaccg ccccactacc
     1801 cgatttatca agatttggcg gagagtcccg tcgactggca aacagctatt atgggtatta
     1861 tgggtgtgta tccagaacga cggatcttga cgcaatttgg cgtggctctc ttaaggtagc
     1921 cctccgacca attgaggtgc gctctgaccg atcagtttag acttgtgtat aagagtcaga
     1981 gcgtcctgaa cggaaccttt cccgttttcc aggatctgat cttccatgtg acctcctaac
     2041 atggtaacgt tcatgataac ttctgctctt catcgtgcgg ccgactgggc taaatctgtg
     2101 ttctcttcgg cggcgctggg tgatcctcgc cgtactgccc gcttggttaa cgtcgccgcc
     2161 caattggcaa aatattctgg taaatcaata accatctcat cagagggtag tgaagccatg
     2221 caggaaggcg cttaccgatt tatccgcaat cccaacgttt ctgccgaggc gatcagaaag
     2281 gctggcgcca tgcaaacagt caagttggct caggagtttc ccgaactgct ggccattgag
     2341 gacaccacct ctttgagtta tcgccaccag gtcgccgaag agcttggcaa gctgggctct
     2401 attcaggata aatcccgcgg atggtgggtt cactccgttc tcttgctcga ggccaccaca
     2461 ttccgcaccg taggattact gcatcaggag tggtggatgc gcccggatga ccctgccgat
     2521 gcggatgaaa aggagagtgg caaatggctg gcagcggccg caactagccg gttacgcatg
     2581 ggcagcatga tgagcaacgt gattgcggtc tgtgaccgcg aagccgatat tcatgcttat
     2641 ctgcaggaca aactggcgca taacgagcgc ttcgtggtgc gctccaagca cccacgcaag
     2701 gacgtagagt ctgggttgta tctgtacgac catctgaaga accaaccgga gttgggtggc
     2761 tatcagatca gcattccgca aaagggcgtg gtggataaac gcggtaaacg taaaaatcga
     2821 ccagcccgca aggcgagctt gagcctgcgc agtgggcgca tcacgctaaa acaggggaat
     2881 atcacgctca acgcggtgct ggccgaggag attaacccgc ccaagggtga gaccccgttg
     2941 aaatggttgt tgctgaccag cgaaccggtc gagtcgctag cccaagcctt gcgcgtcatc
     3001 gacatttata cccatcgctg gcggatcgag gagttccata aggcatggaa aaccggagca
     3061 ggagccgaga ggcaacgcat ggaggagccg gataatctgg agcggatggt ctcgatcctc
     3121 tcgtttgttg cggtcaggct gttacagctc agagaaagct tcacgctgcc gcaagcactc
     3181 agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg
     3241 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa
     3301 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg
     3361 gacagcaagc gaaccggaat tgccagctgg ggcgccctct gggaaggttg ggaagccctg
     3421 caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc
     3481 tgacccggga ctagttggtg tggaagtcca tatatcggtg atcaatatag tggttgacat
     3541 gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca
     3601 aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa
     3661 gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg
     3721 tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata
     3781 catataatac acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa
     3841 attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg
     3901 taaaagttca ctttagcata catatattac acgagccaga aattgtaact tttgcctaaa
     3961 atcacaaatt gcaaaattta attgcttgca aaaggtcaca tgcttataat caactttttt
     4021 aaaaatttaa aatacttttt tattttttat ttttaaacat aaatgaaata atttatttat
     4081 tgtttatgat taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt tgtccttgga
     4141 aaaaaagcac tacctaggag cggccaaaat gccgaggctt tcatagctta aactctttac
     4201 agaaaatagg cattatagat cagttcgagt tttcttattc ttccttccgg ttttatcgtc
     4261 acagttttac agtaaataag tatcacctct tagagttaac tatgagataa gcaagtatca
     4321 tctcatttca tttacctgaa gtcgagtaaa cagaaaatcc aattgttgat gaacctcaat
     4381 gacttagaac tatctatcgg cagatcatat aaagaggatt taggtactcg agaacgctcc
     4441 gtacatgcag ctaaacctcc aagcatctct aacttgggta gtgctttctc caccattttc
     4501 tgtgcttgct ccttcgtggc aagtccagcc cataatgccc agaatgtagt tgcggattcg
     4561 tatgacgttc tgtgcttgat ttttgtgttg tagtcaaaga aaaaccccga ctcgtcatcc
     4621 cacatatatt tggtaattga tgaggcaacg ctaattatca acatatagat tgttatctat
     4681 ctgcatgaac acgaaatctt tacttgacga cttgaggctg atggtgttta tgcaaagaaa
     4741 ccactgtgtt taatatgtgt cactgtttga tattactgtc agcgtagaag ataatagtaa
     4801 aagcggttaa taagtgtatt tgagataagt gtgataaagt ttttacagcg aaaagacgat
     4861 aaatacaaga aaatgattac gaggatacgg agagaggtat gtacatgtgt atttatatac
     4921 taagctgccg gcggttgttt gcaagaccga gaaaaggcta gcaagaatcg ggtcattgta
     4981 gcgtatgcgc ctgtgaacat tctcttcaac aagtttgatt ccattgcggt gaaatggtaa
     5041 aagtcaaccc cctgcgatgt atattttcct gtacaatcaa tcaaaaagcc aaatgattta
     5101 gcattatctt tacatcttgt tattttacag attttatgtt tagatctttt atgcttgctt
     5161 ttcaaaaggc ctgcaggcaa gtgcacaaac aatacttaaa taaatactac tcagtaataa
     5221 cctatttctt agcatttttg acgaaatttg ctattttgtt agagtctttt acaccatttg
     5281 tctccacacc tccgcttaca tcaacaccaa taacgccatt taatctaagc gcatcaccaa
     5341 cattttctgg cgtcagtcca ccagctaaca taaaatgtaa gctctgcctc gcgcgtttcg
     5401 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     5461 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     5521 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     5581 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     5641 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5701 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5761 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5821 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5881 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5941 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6001 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6061 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6121 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6181 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6241 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6301 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6361 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6421 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6481 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6541 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6601 gtctgacagg agctcgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     6661 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     6721 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     6781 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     6841 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt gagcctggcg
     6901 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     6961 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     7021 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     7081 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     7141 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     7201 gccagccacg atagccgcgc tgcctcgtct tggagttcat tcagggcacc ggacaggtcg
     7261 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     7321 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     7381 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     7441 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     7501 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     7561 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     7621 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccgggcg
     7681 atcgatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat
     7741 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg
     7801 tccgccatct ccagcagccg cacgcggcgc atcggggggg gggtttcaat tcaattcatc
     7861 attttttttt tattcttttt tttgatttcg gtttctttga aatttttttg attcggtaat
     7921 ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat
     7981 atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa
     8041 aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac
     8101 tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt
     8161 gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg
     8221 tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg
     8281 cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag
     8341 aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat
     8401 agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg
     8461 tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga
     8521 attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc
     8581 gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga
     8641 tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc
     8701 attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat
     8761 tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag
     8821 aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt
     8881 ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt
     8941 attacccggg aatctcggtc gtaatgattt ttataatgac gaaaaaaaaa aaattggaaa
     9001 gaaaaccccc cccccgcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
     9061 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     9121 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     9181 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
     9241 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
     9301 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
     9361 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
     9421 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatt
     9481 cccccttaca cggaggcatc aagtgaccaa acaggaaaaa accgccctta acatggcccg
     9541 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
     9601 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcaggtgggc
     9661 cattctcatg aagaatatct tgaatttatt gtcatatta
//
