LOCUS       pYTRW07K_0G5           10451 bp    DNA     circular SYN 27-SEP-2021
DEFINITION  
ACCESSION   pYTRW07K_0G5
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     promoter        104..155
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /label=P mob
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P mob"
                     /SECDescr="mob promoter including oriT (Szpirer et al 2001, PMID: 11222611)."
     CDS             223..1242
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID:
                     19928068328)."
                     /transl_table=11
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mob"
                     /SECDescr="Mob protein necessary for mobilization"
     misc_feature    1243..1261
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-L
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-L"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     misc_feature    1262..1301
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /label=Core_up
                     /SECDrawAs="Label"
                     /SECName="Core_up"
                     /SECDescr="upstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    1302..1316
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    1317..1356
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /SECDrawAs="Label"
                     /SECName="P_up"
                     /SECDescr="upstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1357..1393
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /label=PI-SceI
                     /SECDrawAs="Label"
                     /SECName="PI-SceI"
                     /SECDescr="recognition site of homing endonuclease PI-SceI, enabling e.g. promoter insertion."
     misc_feature    1394..1401
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /label=AsiSI
                     /SECDrawAs="Label"
                     /SECName="AsiSI"
                     /SECDescr="recognition site of endonuclease AsiSI, enabling e.g. promoter insertion."
     misc_feature    1402..1441
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /label=P_dn
                     /SECDrawAs="Label"
                     /SECName="P_dn"
                     /SECDescr="downstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1402..1521
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_up
                     /SECDrawAs="Label"
                     /SECName="CIS_up"
                     /SECDescr="upstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1522..1539
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /label=I-SceI
                     /SECDrawAs="Label"
                     /SECName="I-SceI"
                     /SECDescr="recognition site of homing endonuclease I-SceI, enabling target gene insertion."
     misc_feature    1540..1659
                     /note="downstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_dn
                     /SECDrawAs="Label"
                     /SECName="CIS_dn"
                     /SECDescr="downstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1620..1659
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /gene="R_up"
                     /SECDrawAs="Label"
                     /SECName="R_up"
                     /SECDescr="upstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    1660..1667
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion."
                     /label=MauBI
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion."
     misc_feature    1668..1693
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion."
                     /label=I-CeuI
                     /SECDrawAs="Label"
                     /SECName="I-CeuI"
                     /SECDescr="recognition site of homing endonuclease I-CeuI, enabling e.g. reporter insertion."
     misc_feature    1694..1733
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /label=R_dn
                     /SECDrawAs="Label"
                     /SECName="R_dn"
                     /SECDescr="downstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    1733..1790
                     /label=MCS Integration site
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /SECDrawAs="Info only"
     misc_feature    1734..1789
                     /note="region with Multiple Cutters
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon
                     Tn5 insertion site elucidation."
                     /label=MC
                     /SECDrawAs="Label"
                     /SECName="MC"
                     /SECDescr="region with Multiple Cutters EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon Tn5 insertion site elucidation."
     misc_feature    1790..1829
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_up
                     /SECDrawAs="Label"
                     /SECName="M_up"
                     /SECDescr="upstream sequence of marker slot (SalI/PI-PspI)."
     mobile_element  complement(1830..2581)
                     /mobile_element_type="transposon:mini-Tn7"
                     /SECDrawAs="Info only"
                     /SECName="'mobile_element'"
     source          complement(1830..2581)
                     /organism="Cloning vector pUC18-mini-Tn7-Gm"
                     /mol_type="other DNA"
                     /db_xref="taxon:295022"
                     /SECDrawAs="Info only"
                     /SECName="'source'"
     promoter        1831..1860
                     /note="aacC1 promoter region (Wohlleben et al 1989, PMID:
                     2549372)."
                     /label=P aacC1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aacC1"
                     /SECDescr="aacC1 promoter region (Wohlleben et al 1989, PMID: 2549372)."
     CDS             2048..2581
                     /gene="aacC1"
                     /transl_table=11
                     /product="gentamicin acetyl transferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL
                     DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQP
                     RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL
                     GIREEVMHFDIDPSTAT"
                     /note="aacC1 from pUCGM (Schweizer 1993, PMID: 8267974)."
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aacC1"
                     /SECDescr="gentamicin acetyl transferase"
     gene            2048..2581
                     /gene="aacC1"
                     /SECDrawAs="Info only"
                     /SECName="aacC1"
     misc_feature    2582..2587
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /label=SalI
                     /SECDrawAs="Label"
                     /SECName="SalI"
                     /SECDescr="recognition site of endonuclease SalI, enabling e.g. marker insertion."
     misc_feature    2588..2617
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /label=PI-PspI
                     /SECDrawAs="Label"
                     /SECName="PI-PspI"
                     /SECDescr="recognition site of homing endonuclease PI-PspI, enabling e.g. marker insertion."
     misc_feature    2618..2657
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_dn
                     /SECDrawAs="Label"
                     /SECName="M_dn"
                     /SECDescr="downstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    2658..2672
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    2673..2712
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /label=Core_dn
                     /SECDrawAs="Label"
                     /SECName="Core_dn"
                     /SECDescr="downstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    complement(2713..2731)
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-R
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-R"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     promoter        2742..2772
                     /note="transposon Tn5 transposase promoter (Yin et al
                     1988, PMID: 2451025)."
                     /label=P tnp
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tnp"
                     /SECDescr="transposon Tn5 transposase promoter (Yin et al 1988, PMID: 2451025)."
     CDS             2805..4235
                     /gene="tnp"
                     /product="transposon Tn5 transposase"
                     /transl_table=11
                     /note="transposon Tn5 transposase (Goryshin & Reznikoff
                     1998, PMID: 9516433)."
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGK
                     SITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLS
                     YRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEK
                     ESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDV
                     ESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGN
                     ITLNAVLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKT
                     GAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQS
                     AETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW
                     EGWEALQSKLDGFLAAKDLMAQGIKI"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tnp"
                     /SECDescr="transposon Tn5 transposase"
     misc_feature    4747..4857
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /label=CEN4
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="CEN4"
                     /SECDescr="CEN4 of Saccharomyces cerevisiae Chr IV (Saccharomyces Genome Database)."
     misc_feature    5389..6133
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /label=ARS1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ARS1 "
                     /SECDescr="ARS1 of yCP50 (Genbank: X70276.1)."
     rep_origin      6603..7191
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387);
                     annotated as New England Biolabs (2007)."
                     /label=pMB1 ori
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pMB1 ori"
                     /SECDescr="origin of replication from plasmid pBR322 (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); annotated as New England Biolabs (2007)."
     CDS             complement(7370..8164)
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /transl_table=11
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aphII"
                     /SECDescr="aminoglycoside 3'-phosphotransferase"
     promoter        8254..8302
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank
                     U00004.1)."
                     /label=P aphII
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aphII"
                     /SECDescr="promoter of aphII gene in Transposon Tn5 (Genbank U00004.1)."
     promoter        8602..8817
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /label=P URA3
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P URA3"
                     /SECDescr="URA3 promoter region (Roy et al 1990, PMID: 2204810)."
     CDS             8818..9621
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="URA3"
                     /SECDescr="orotidine 5'-phosphate decarboxylase"
ORIGIN      
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgtaa ctataacggt
     1681 cctaaggtag cgagcttact tgtgctgttg tgtaaggaca cgcttaaccc agagaattcc
     1741 agcgacctat tctacgacta cgcgtccatg gctcgaggag ctcggtaccg ccccactacc
     1801 cgatttatca agatttggcg gagagtccca ttgacataag cctgttcggt tcgtaaactg
     1861 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     1921 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg
     1981 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     2041 agcaacgatg ttacgcagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     2101 gttaggtggc tcaagtatgg gcatcattcg cacatgtagg ctcggccctg accaagtcaa
     2161 atccatgcgg gctgctcttg atcttttcgg tcgtgagttc ggagacgtag ccacctactc
     2221 ccaacatcag ccggactccg attacctcgg gaacttgctc cgtagtaaga cattcatcgc
     2281 gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc gcggcttacg ttctgcccaa
     2341 gtttgagcag ccgcgtagtg agatctatat ctatgatctc gcagtctccg gcgagcaccg
     2401 gaggcagggc attgccaccg cgctcatcaa tctcctcaag catgaggcca acgcgcttgg
     2461 tgcttatgtg atctacgtgc aagcagatta cggtgacgat cccgcagtgg ctctctatac
     2521 aaagttgggc atacgggaag aagtgatgca ctttgatatc gacccaagta ccgccaccta
     2581 agtcgactgg caaacagcta ttatgggtat tatgggtgtg tatccagaac gacggatctt
     2641 gacgcaattt ggcgtggctc tcttaaggta gccctccgac caattgaggt gcgctctgac
     2701 cgatcagttt agacttgtgt ataagagtca gagcgtcctg aacggaacct ttcccgtttt
     2761 ccaggatctg atcttccatg tgacctccta acatggtaac gttcatgata acttctgctc
     2821 ttcatcgtgc ggccgactgg gctaaatctg tgttctcttc ggcggcgctg ggtgatcctc
     2881 gccgtactgc ccgcttggtt aacgtcgccg cccaattggc aaaatattct ggtaaatcaa
     2941 taaccatctc atcagagggt agtgaagcca tgcaggaagg cgcttaccga tttatccgca
     3001 atcccaacgt ttctgccgag gcgatcagaa aggctggcgc catgcaaaca gtcaagttgg
     3061 ctcaggagtt tcccgaactg ctggccattg aggacaccac ctctttgagt tatcgccacc
     3121 aggtcgccga agagcttggc aagctgggct ctattcagga taaatcccgc ggatggtggg
     3181 ttcactccgt tctcttgctc gaggccacca cattccgcac cgtaggatta ctgcatcagg
     3241 agtggtggat gcgcccggat gaccctgccg atgcggatga aaaggagagt ggcaaatggc
     3301 tggcagcggc cgcaactagc cggttacgca tgggcagcat gatgagcaac gtgattgcgg
     3361 tctgtgaccg cgaagccgat attcatgctt atctgcagga caaactggcg cataacgagc
     3421 gcttcgtggt gcgctccaag cacccacgca aggacgtaga gtctgggttg tatctgtacg
     3481 accatctgaa gaaccaaccg gagttgggtg gctatcagat cagcattccg caaaagggcg
     3541 tggtggataa acgcggtaaa cgtaaaaatc gaccagcccg caaggcgagc ttgagcctgc
     3601 gcagtgggcg catcacgcta aaacagggga atatcacgct caacgcggtg ctggccgagg
     3661 agattaaccc gcccaagggt gagaccccgt tgaaatggtt gttgctgacc agcgaaccgg
     3721 tcgagtcgct agcccaagcc ttgcgcgtca tcgacattta tacccatcgc tggcggatcg
     3781 aggagttcca taaggcatgg aaaaccggag caggagccga gaggcaacgc atggaggagc
     3841 cggataatct ggagcggatg gtctcgatcc tctcgtttgt tgcggtcagg ctgttacagc
     3901 tcagagaaag cttcacgctg ccgcaagcac tcagggcgca agggctgcta aaggaagcgg
     3961 aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg
     4021 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt
     4081 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct
     4141 ggggcgccct ctgggaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
     4201 ccaaggatct gatggcgcag gggatcaaga tctgacccgg gactagttgg tgtggaagtc
     4261 catatatcgg tgatcaatat agtggttgac atgctggcta gtcaacattg agccttttga
     4321 tcatgcaaat atattacggt attttacaat caaatatcaa acttaactat tgactttata
     4381 acttatttag gtggtaacat tcttataaaa aagaaaaaaa ttactgcaaa acagtactag
     4441 cttttaactt gtatcctagg ttatctatgc tgtctcacca tagagaatat tacctatttc
     4501 agaatgtatg tccatgattc gccgggtaaa tacatataat acacaaatct ggcttaataa
     4561 agtctataat atatctcata aagaagtgct aaattggcta gtgctatata tttttaagaa
     4621 aatttctttt gactaagtcc atatcgactt tgtaaaagtt cactttagca tacatatatt
     4681 acacgagcca gaaattgtaa cttttgccta aaatcacaaa ttgcaaaatt taattgcttg
     4741 caaaaggtca catgcttata atcaactttt ttaaaaattt aaaatacttt tttatttttt
     4801 atttttaaac ataaatgaaa taatttattt attgtttatg attaccgaaa cataaaacct
     4861 gctcaagaaa aagaaactgt tttgtccttg gaaaaaaagc actacctagg agcggccaaa
     4921 atgccgaggc tttcatagct taaactcttt acagaaaata ggcattatag atcagttcga
     4981 gttttcttat tcttccttcc ggttttatcg tcacagtttt acagtaaata agtatcacct
     5041 cttagagtta actatgagat aagcaagtat catctcattt catttacctg aagtcgagta
     5101 aacagaaaat ccaattgttg atgaacctca atgacttaga actatctatc ggcagatcat
     5161 ataaagagga tttaggtact cgagaacgct ccgtacatgc agctaaacct ccaagcatct
     5221 ctaacttggg tagtgctttc tccaccattt tctgtgcttg ctccttcgtg gcaagtccag
     5281 cccataatgc ccagaatgta gttgcggatt cgtatgacgt tctgtgcttg atttttgtgt
     5341 tgtagtcaaa gaaaaacccc gactcgtcat cccacatata tttggtaatt gatgaggcaa
     5401 cgctaattat caacatatag attgttatct atctgcatga acacgaaatc tttacttgac
     5461 gacttgaggc tgatggtgtt tatgcaaaga aaccactgtg tttaatatgt gtcactgttt
     5521 gatattactg tcagcgtaga agataatagt aaaagcggtt aataagtgta tttgagataa
     5581 gtgtgataaa gtttttacag cgaaaagacg ataaatacaa gaaaatgatt acgaggatac
     5641 ggagagaggt atgtacatgt gtatttatat actaagctgc cggcggttgt ttgcaagacc
     5701 gagaaaaggc tagcaagaat cgggtcattg tagcgtatgc gcctgtgaac attctcttca
     5761 acaagtttga ttccattgcg gtgaaatggt aaaagtcaac cccctgcgat gtatattttc
     5821 ctgtacaatc aatcaaaaag ccaaatgatt tagcattatc tttacatctt gttattttac
     5881 agattttatg tttagatctt ttatgcttgc ttttcaaaag gcctgcaggc aagtgcacaa
     5941 acaatactta aataaatact actcagtaat aacctatttc ttagcatttt tgacgaaatt
     6001 tgctattttg ttagagtctt ttacaccatt tgtctccaca cctccgctta catcaacacc
     6061 aataacgcca tttaatctaa gcgcatcacc aacattttct ggcgtcagtc caccagctaa
     6121 cataaaatgt aagctctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     6181 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     6241 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     6301 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     6361 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     6421 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6481 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6541 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6601 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6661 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6721 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6781 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6841 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6901 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6961 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7021 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7081 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7141 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7201 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7261 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7321 aaatcaatct aaagtatata tgagtaaact tggtctgaca ggagctcgct cagaagaact
     7381 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     7441 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     7501 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     7561 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
     7621 cgccgtcggg catccgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     7681 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     7741 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     7801 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     7861 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     7921 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     7981 cttggagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     8041 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     8101 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     8161 tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga
     8221 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
     8281 gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc
     8341 gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc
     8401 agatagccca gtagctgaca ttcatccggg cgatcgatgg aagccggcgg cacctcgcta
     8461 acggattcac cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc
     8521 aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc
     8581 gcatcggggg gggggtttca attcaattca tcattttttt tttattcttt tttttgattt
     8641 cggtttcttt gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag
     8701 gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac atgaaattgc
     8761 ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag ataaatcatg
     8821 tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc tgccaagcta
     8881 tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc
     8941 aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact aaaaacacat
     9001 gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa ggcattatcc
     9061 gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg taatacagtc
     9121 aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat tacgaatgca
     9181 cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga agaagtaaca
     9241 aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc cctatctact
     9301 ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt tgttatcggc
     9361 tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt gattatgaca
     9421 cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag aaccgtggat
     9481 gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt tgcaaaggga
     9541 agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc atatttgaga
     9601 agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat actaaactca
     9661 caaattagag cttcaattta attatatcag ttattacccg ggaatctcgg tcgtaatgat
     9721 ttttataatg acgaaaaaaa aaaaattgga aagaaaaccc cccccccgca gcgttgggtc
     9781 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     9841 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     9901 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     9961 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
    10021 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
    10081 ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
    10141 ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
    10201 catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
    10261 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
    10321 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
    10381 gaccacgctg atgagcttta ccgcaggtgg gccattctca tgaagaatat cttgaattta
    10441 ttgtcatatt a
//
