LOCUS       pYTRW08K_0C5           10607 bp    DNA     circular SYN 27-SEP-2021
DEFINITION  
ACCESSION   pYTRW08K_0C5
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     promoter        104..155
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /label=P mob
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P mob"
                     /SECDescr="mob promoter including oriT (Szpirer et al 2001, PMID: 11222611)."
     CDS             223..1242
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID:
                     19928068328)."
                     /transl_table=11
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mob"
                     /SECDescr="Mob protein necessary for mobilization"
     misc_feature    1243..1261
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-L
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-L"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     misc_feature    1262..1301
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /label=Core_up
                     /SECDrawAs="Label"
                     /SECName="Core_up"
                     /SECDescr="upstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    1302..1316
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    1317..1356
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /SECDrawAs="Label"
                     /SECName="P_up"
                     /SECDescr="upstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1357..1393
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /label=PI-SceI
                     /SECDrawAs="Label"
                     /SECName="PI-SceI"
                     /SECDescr="recognition site of homing endonuclease PI-SceI, enabling e.g. promoter insertion."
     misc_feature    1394..1401
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /label=AsiSI
                     /SECDrawAs="Label"
                     /SECName="AsiSI"
                     /SECDescr="recognition site of endonuclease AsiSI, enabling e.g. promoter insertion."
     misc_feature    1402..1441
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /label=P_dn
                     /SECDrawAs="Label"
                     /SECName="P_dn"
                     /SECDescr="downstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1402..1521
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_up
                     /SECDrawAs="Label"
                     /SECName="CIS_up"
                     /SECDescr="upstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1522..1539
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /label=I-SceI
                     /SECDrawAs="Label"
                     /SECName="I-SceI"
                     /SECDescr="recognition site of homing endonuclease I-SceI, enabling target gene insertion."
     misc_feature    1540..1659
                     /note="downstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_dn
                     /SECDrawAs="Label"
                     /SECName="CIS_dn"
                     /SECDescr="downstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1620..1659
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /gene="R_up"
                     /SECDrawAs="Label"
                     /SECName="R_up"
                     /SECDescr="upstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    1660..1667
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion."
                     /label=MauBI
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion."
     misc_feature    1668..1693
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion."
                     /label=I-CeuI
                     /SECDrawAs="Label"
                     /SECName="I-CeuI"
                     /SECDescr="recognition site of homing endonuclease I-CeuI, enabling e.g. reporter insertion."
     misc_feature    1694..1733
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /label=R_dn
                     /SECDrawAs="Label"
                     /SECName="R_dn"
                     /SECDescr="downstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    1733..1790
                     /label=MCS Integration site
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /SECDrawAs="Info only"
     misc_feature    1734..1789
                     /note="region with Multiple Cutters
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon
                     Tn5 insertion site elucidation."
                     /label=MC
                     /SECDrawAs="Label"
                     /SECName="MC"
                     /SECDescr="region with Multiple Cutters EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon Tn5 insertion site elucidation."
     misc_feature    1790..1829
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_up
                     /SECDrawAs="Label"
                     /SECName="M_up"
                     /SECDescr="upstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    1849..2028
                     /label=P cat
                     /note="cat promoter region (Grice et al 1982, PMID:
                     7037748)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P cat"
                     /SECDescr="cat promoter region (Grice et al 1982, PMID: 7037748)."
     CDS             2057..2827
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /transl_table=11
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQFLCMRPIR
                     KPPLPARWPVDWQTAIMGIMGVYPERRILTQFGVALLR"
                     /note="CmR from plasmid pRhotHi-2 (Katzke et al 2010,
                     PMID: 19706327), native up to Q210 as in pBR325 (Bolivar
                     1978, PMID: 363519), and up to P227 as in pBBR1MCS (Kovach
                     et al 1994, PMID: 8068328)."
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="cat"
                     /SECDescr="chloramphenicol acetyltransferase"
     misc_feature    2738..2743
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /label=SalI
                     /SECDrawAs="Label"
                     /SECName="SalI"
                     /SECDescr="recognition site of endonuclease SalI, enabling e.g. marker insertion."
     misc_feature    2744..2773
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /label=PI-PspI
                     /SECDrawAs="Label"
                     /SECName="PI-PspI"
                     /SECDescr="recognition site of homing endonuclease PI-PspI, enabling e.g. marker insertion."
     misc_feature    2774..2813
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_dn
                     /SECDrawAs="Label"
                     /SECName="M_dn"
                     /SECDescr="downstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    2814..2828
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    2829..2868
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /label=Core_dn
                     /SECDrawAs="Label"
                     /SECName="Core_dn"
                     /SECDescr="downstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    complement(2869..2887)
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-R
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-R"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     promoter        2898..2928
                     /note="transposon Tn5 transposase promoter (Yin et al
                     1988, PMID: 2451025)."
                     /label=P tnp
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tnp"
                     /SECDescr="transposon Tn5 transposase promoter (Yin et al 1988, PMID: 2451025)."
     CDS             2961..4391
                     /gene="tnp"
                     /product="transposon Tn5 transposase"
                     /transl_table=11
                     /note="transposon Tn5 transposase (Goryshin & Reznikoff
                     1998, PMID: 9516433)."
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGK
                     SITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLS
                     YRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEK
                     ESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDV
                     ESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGN
                     ITLNAVLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKT
                     GAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQS
                     AETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW
                     EGWEALQSKLDGFLAAKDLMAQGIKI"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tnp"
                     /SECDescr="transposon Tn5 transposase"
     misc_feature    4903..5013
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /label=CEN4
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="CEN4"
                     /SECDescr="CEN4 of Saccharomyces cerevisiae Chr IV (Saccharomyces Genome Database)."
     misc_feature    5545..6289
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /label=ARS1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ARS1 "
                     /SECDescr="ARS1 of yCP50 (Genbank: X70276.1)."
     rep_origin      6759..7347
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387);
                     annotated as New England Biolabs (2007)."
                     /label=pMB1 ori
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pMB1 ori"
                     /SECDescr="origin of replication from plasmid pBR322 (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); annotated as New England Biolabs (2007)."
     CDS             complement(7526..8320)
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /transl_table=11
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aphII"
                     /SECDescr="aminoglycoside 3'-phosphotransferase"
     promoter        8410..8458
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank
                     U00004.1)."
                     /label=P aphII
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aphII"
                     /SECDescr="promoter of aphII gene in Transposon Tn5 (Genbank U00004.1)."
     promoter        8758..8973
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /label=P URA3
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P URA3"
                     /SECDescr="URA3 promoter region (Roy et al 1990, PMID: 2204810)."
     CDS             8974..9777
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="URA3"
                     /SECDescr="orotidine 5'-phosphate decarboxylase"
ORIGIN      
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgtaa ctataacggt
     1681 cctaaggtag cgagcttact tgtgctgttg tgtaaggaca cgcttaaccc agagaattcc
     1741 agcgacctat tctacgacta cgcgtccatg gctcgaggag ctcggtaccg ccccactacc
     1801 cgatttatca agatttggcg gagagtcccc acgaggccct ttcgtcttcg aataaatacc
     1861 tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa
     1921 gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag gttccaactt
     1981 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
     2041 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     2101 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     2161 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     2221 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     2281 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     2341 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     2401 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     2461 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     2521 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     2581 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     2641 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtttt tatgcatgcg
     2701 cccaatacgc aaaccgcctc tccccgcgcg ttggccggtc gactggcaaa cagctattat
     2761 gggtattatg ggtgtgtatc cagaacgacg gatcttgacg caatttggcg tggctctctt
     2821 aaggtagccc tccgaccaat tgaggtgcgc tctgaccgat cagtttagac ttgtgtataa
     2881 gagtcagagc gtcctgaacg gaacctttcc cgttttccag gatctgatct tccatgtgac
     2941 ctcctaacat ggtaacgttc atgataactt ctgctcttca tcgtgcggcc gactgggcta
     3001 aatctgtgtt ctcttcggcg gcgctgggtg atcctcgccg tactgcccgc ttggttaacg
     3061 tcgccgccca attggcaaaa tattctggta aatcaataac catctcatca gagggtagtg
     3121 aagccatgca ggaaggcgct taccgattta tccgcaatcc caacgtttct gccgaggcga
     3181 tcagaaaggc tggcgccatg caaacagtca agttggctca ggagtttccc gaactgctgg
     3241 ccattgagga caccacctct ttgagttatc gccaccaggt cgccgaagag cttggcaagc
     3301 tgggctctat tcaggataaa tcccgcggat ggtgggttca ctccgttctc ttgctcgagg
     3361 ccaccacatt ccgcaccgta ggattactgc atcaggagtg gtggatgcgc ccggatgacc
     3421 ctgccgatgc ggatgaaaag gagagtggca aatggctggc agcggccgca actagccggt
     3481 tacgcatggg cagcatgatg agcaacgtga ttgcggtctg tgaccgcgaa gccgatattc
     3541 atgcttatct gcaggacaaa ctggcgcata acgagcgctt cgtggtgcgc tccaagcacc
     3601 cacgcaagga cgtagagtct gggttgtatc tgtacgacca tctgaagaac caaccggagt
     3661 tgggtggcta tcagatcagc attccgcaaa agggcgtggt ggataaacgc ggtaaacgta
     3721 aaaatcgacc agcccgcaag gcgagcttga gcctgcgcag tgggcgcatc acgctaaaac
     3781 aggggaatat cacgctcaac gcggtgctgg ccgaggagat taacccgccc aagggtgaga
     3841 ccccgttgaa atggttgttg ctgaccagcg aaccggtcga gtcgctagcc caagccttgc
     3901 gcgtcatcga catttatacc catcgctggc ggatcgagga gttccataag gcatggaaaa
     3961 ccggagcagg agccgagagg caacgcatgg aggagccgga taatctggag cggatggtct
     4021 cgatcctctc gtttgttgcg gtcaggctgt tacagctcag agaaagcttc acgctgccgc
     4081 aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag
     4141 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca
     4201 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg
     4261 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg gaaggttggg
     4321 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga
     4381 tcaagatctg acccgggact agttggtgtg gaagtccata tatcggtgat caatatagtg
     4441 gttgacatgc tggctagtca acattgagcc ttttgatcat gcaaatatat tacggtattt
     4501 tacaatcaaa tatcaaactt aactattgac tttataactt atttaggtgg taacattctt
     4561 ataaaaaaga aaaaaattac tgcaaaacag tactagcttt taacttgtat cctaggttat
     4621 ctatgctgtc tcaccataga gaatattacc tatttcagaa tgtatgtcca tgattcgccg
     4681 ggtaaataca tataatacac aaatctggct taataaagtc tataatatat ctcataaaga
     4741 agtgctaaat tggctagtgc tatatatttt taagaaaatt tcttttgact aagtccatat
     4801 cgactttgta aaagttcact ttagcataca tatattacac gagccagaaa ttgtaacttt
     4861 tgcctaaaat cacaaattgc aaaatttaat tgcttgcaaa aggtcacatg cttataatca
     4921 acttttttaa aaatttaaaa tactttttta ttttttattt ttaaacataa atgaaataat
     4981 ttatttattg tttatgatta ccgaaacata aaacctgctc aagaaaaaga aactgttttg
     5041 tccttggaaa aaaagcacta cctaggagcg gccaaaatgc cgaggctttc atagcttaaa
     5101 ctctttacag aaaataggca ttatagatca gttcgagttt tcttattctt ccttccggtt
     5161 ttatcgtcac agttttacag taaataagta tcacctctta gagttaacta tgagataagc
     5221 aagtatcatc tcatttcatt tacctgaagt cgagtaaaca gaaaatccaa ttgttgatga
     5281 acctcaatga cttagaacta tctatcggca gatcatataa agaggattta ggtactcgag
     5341 aacgctccgt acatgcagct aaacctccaa gcatctctaa cttgggtagt gctttctcca
     5401 ccattttctg tgcttgctcc ttcgtggcaa gtccagccca taatgcccag aatgtagttg
     5461 cggattcgta tgacgttctg tgcttgattt ttgtgttgta gtcaaagaaa aaccccgact
     5521 cgtcatccca catatatttg gtaattgatg aggcaacgct aattatcaac atatagattg
     5581 ttatctatct gcatgaacac gaaatcttta cttgacgact tgaggctgat ggtgtttatg
     5641 caaagaaacc actgtgttta atatgtgtca ctgtttgata ttactgtcag cgtagaagat
     5701 aatagtaaaa gcggttaata agtgtatttg agataagtgt gataaagttt ttacagcgaa
     5761 aagacgataa atacaagaaa atgattacga ggatacggag agaggtatgt acatgtgtat
     5821 ttatatacta agctgccggc ggttgtttgc aagaccgaga aaaggctagc aagaatcggg
     5881 tcattgtagc gtatgcgcct gtgaacattc tcttcaacaa gtttgattcc attgcggtga
     5941 aatggtaaaa gtcaaccccc tgcgatgtat attttcctgt acaatcaatc aaaaagccaa
     6001 atgatttagc attatcttta catcttgtta ttttacagat tttatgttta gatcttttat
     6061 gcttgctttt caaaaggcct gcaggcaagt gcacaaacaa tacttaaata aatactactc
     6121 agtaataacc tatttcttag catttttgac gaaatttgct attttgttag agtcttttac
     6181 accatttgtc tccacacctc cgcttacatc aacaccaata acgccattta atctaagcgc
     6241 atcaccaaca ttttctggcg tcagtccacc agctaacata aaatgtaagc tctgcctcgc
     6301 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     6361 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     6421 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     6481 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     6541 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     6601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7501 taaacttggt ctgacaggag ctcgctcaga agaactcgtc aagaaggcga tagaaggcga
     7561 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     7621 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     7681 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     7741 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatc cgcgccttga
     7801 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     7861 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     7921 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     7981 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     8041 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     8101 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg gagttcattc agggcaccgg
     8161 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     8221 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     8281 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     8341 tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc
     8401 agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt
     8461 cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta
     8521 cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca
     8581 tccgggcgat cgatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg
     8641 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat
     8701 ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat cggggggggg gtttcaattc
     8761 aattcatcat ttttttttta ttcttttttt tgatttcggt ttctttgaaa tttttttgat
     8821 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
     8881 atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
     8941 agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt
     9001 gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa
     9061 acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa
     9121 gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc
     9181 atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc
     9241 gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta
     9301 tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt
     9361 gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg
     9421 ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt
     9481 gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt
     9541 ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag
     9601 ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac
     9661 attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa
     9721 cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa
     9781 actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta
     9841 tatcagttat tacccgggaa tctcggtcgt aatgattttt ataatgacga aaaaaaaaaa
     9901 attggaaaga aaaccccccc cccgcagcgt tgggtcctgg ccacgggtgc gcatgatcgt
     9961 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga
    10021 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc
    10081 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc
    10141 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
    10201 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg
    10261 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc
    10321 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa
    10381 cagaaattcc cccttacacg gaggcatcaa gtgaccaaac aggaaaaaac cgcccttaac
    10441 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac
    10501 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc
    10561 aggtgggcca ttctcatgaa gaatatcttg aatttattgt catatta
//
