LOCUS       pYTRW09K_0T5           10972 bp    DNA     circular SYN 27-SEP-2021
DEFINITION  
ACCESSION   pYTRW09K_0T5
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     promoter        104..155
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /label=P mob
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P mob"
                     /SECDescr="mob promoter including oriT (Szpirer et al 2001, PMID: 11222611)."
     CDS             223..1242
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID:
                     19928068328)."
                     /transl_table=11
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mob"
                     /SECDescr="Mob protein necessary for mobilization"
     misc_feature    1243..1261
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-L
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-L"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     misc_feature    1262..1301
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /label=Core_up
                     /SECDrawAs="Label"
                     /SECName="Core_up"
                     /SECDescr="upstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    1302..1316
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    1317..1356
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /SECDrawAs="Label"
                     /SECName="P_up"
                     /SECDescr="upstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1357..1393
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /label=PI-SceI
                     /SECDrawAs="Label"
                     /SECName="PI-SceI"
                     /SECDescr="recognition site of homing endonuclease PI-SceI, enabling e.g. promoter insertion."
     misc_feature    1394..1401
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /label=AsiSI
                     /SECDrawAs="Label"
                     /SECName="AsiSI"
                     /SECDescr="recognition site of endonuclease AsiSI, enabling e.g. promoter insertion."
     misc_feature    1402..1441
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /label=P_dn
                     /SECDrawAs="Label"
                     /SECName="P_dn"
                     /SECDescr="downstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1402..1521
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_up
                     /SECDrawAs="Label"
                     /SECName="CIS_up"
                     /SECDescr="upstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1522..1539
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /label=I-SceI
                     /SECDrawAs="Label"
                     /SECName="I-SceI"
                     /SECDescr="recognition site of homing endonuclease I-SceI, enabling target gene insertion."
     misc_feature    1540..1659
                     /note="downstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_dn
                     /SECDrawAs="Label"
                     /SECName="CIS_dn"
                     /SECDescr="downstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1620..1659
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /gene="R_up"
                     /SECDrawAs="Label"
                     /SECName="R_up"
                     /SECDescr="upstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    1660..1667
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion."
                     /label=MauBI
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion."
     misc_feature    1668..1693
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion."
                     /label=I-CeuI
                     /SECDrawAs="Label"
                     /SECName="I-CeuI"
                     /SECDescr="recognition site of homing endonuclease I-CeuI, enabling e.g. reporter insertion."
     misc_feature    1694..1733
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /label=R_dn
                     /SECDrawAs="Label"
                     /SECName="R_dn"
                     /SECDescr="downstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    1733..1790
                     /label=MCS Integration site
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /SECDrawAs="Info only"
     misc_feature    1734..1789
                     /note="region with Multiple Cutters
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon
                     Tn5 insertion site elucidation."
                     /label=MC
                     /SECDrawAs="Label"
                     /SECName="MC"
                     /SECDescr="region with Multiple Cutters EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon Tn5 insertion site elucidation."
     misc_feature    1790..1829
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_up
                     /SECDrawAs="Label"
                     /SECName="M_up"
                     /SECDescr="upstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    1830..1889
                     /label=P tet
                     /note="tet promoter region (Harley et al 1988, PMID:
                     3045754)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tet"
                     /SECDescr="tet promoter region (Harley et al 1988, PMID: 3045754)."
     CDS             1912..3102
                     /gene="tet"
                     /product="tetracycline effux pump"
                     /transl_table=11
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
                     /note="tet gene, TcR from pBR322 (Bolivar 1977, PMID:
                     344137; Sutcliffe 1979, PMID: 383387)."
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tet"
                     /SECDescr="tetracycline effux pump"
     misc_feature    3103..3108
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /label=SalI
                     /SECDrawAs="Label"
                     /SECName="SalI"
                     /SECDescr="recognition site of endonuclease SalI, enabling e.g. marker insertion."
     misc_feature    3109..3138
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /label=PI-PspI
                     /SECDrawAs="Label"
                     /SECName="PI-PspI"
                     /SECDescr="recognition site of homing endonuclease PI-PspI, enabling e.g. marker insertion."
     misc_feature    3139..3178
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_dn
                     /SECDrawAs="Label"
                     /SECName="M_dn"
                     /SECDescr="downstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    3179..3193
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    3194..3233
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /label=Core_dn
                     /SECDrawAs="Label"
                     /SECName="Core_dn"
                     /SECDescr="downstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    complement(3234..3252)
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-R
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-R"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     promoter        3263..3293
                     /note="transposon Tn5 transposase promoter (Yin et al
                     1988, PMID: 2451025)."
                     /label=P tnp
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tnp"
                     /SECDescr="transposon Tn5 transposase promoter (Yin et al 1988, PMID: 2451025)."
     CDS             3326..4756
                     /gene="tnp"
                     /product="transposon Tn5 transposase"
                     /transl_table=11
                     /note="transposon Tn5 transposase (Goryshin & Reznikoff
                     1998, PMID: 9516433)."
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGK
                     SITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLS
                     YRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEK
                     ESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDV
                     ESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGN
                     ITLNAVLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKT
                     GAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQS
                     AETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW
                     EGWEALQSKLDGFLAAKDLMAQGIKI"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tnp"
                     /SECDescr="transposon Tn5 transposase"
     misc_feature    5268..5378
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /label=CEN4
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="CEN4"
                     /SECDescr="CEN4 of Saccharomyces cerevisiae Chr IV (Saccharomyces Genome Database)."
     misc_feature    5910..6654
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /label=ARS1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ARS1 "
                     /SECDescr="ARS1 of yCP50 (Genbank: X70276.1)."
     rep_origin      7124..7712
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387);
                     annotated as New England Biolabs (2007)."
                     /label=ori pMB1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pMB1 ori"
                     /SECDescr="origin of replication from plasmid pBR322 (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); annotated as New England Biolabs (2007)."
     CDS             complement(7891..8685)
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /transl_table=11
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aphII"
                     /SECDescr="aminoglycoside 3'-phosphotransferase"
     promoter        8775..8823
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank
                     U00004.1)."
                     /label=P aphII
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aphII"
                     /SECDescr="promoter of aphII gene in Transposon Tn5 (Genbank U00004.1)."
     promoter        9123..9338
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /label=P URA3
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P URA3"
                     /SECDescr="URA3 promoter region (Roy et al 1990, PMID: 2204810)."
     CDS             9339..10142
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="URA3"
                     /SECDescr="orotidine 5'-phosphate decarboxylase"
ORIGIN      
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgtaa ctataacggt
     1681 cctaaggtag cgagcttact tgtgctgttg tgtaaggaca cgcttaaccc agagaattcc
     1741 agcgacctat tctacgacta cgcgtccatg gctcgaggag ctcggtaccg ccccactacc
     1801 cgatttatca agatttggcg gagagtccct catgtttgac agcttatcat cgataagctt
     1861 taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct
     1921 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
     1981 gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
     2041 cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
     2101 ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
     2161 actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc
     2221 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     2281 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     2341 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     2401 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     2461 tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     2521 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     2581 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     2641 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     2701 gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     2761 atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     2821 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     2881 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     2941 accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     3001 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     3061 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gagtcgactg gcaaacagct
     3121 attatgggta ttatgggtgt gtatccagaa cgacggatct tgacgcaatt tggcgtggct
     3181 ctcttaaggt agccctccga ccaattgagg tgcgctctga ccgatcagtt tagacttgtg
     3241 tataagagtc agagcgtcct gaacggaacc tttcccgttt tccaggatct gatcttccat
     3301 gtgacctcct aacatggtaa cgttcatgat aacttctgct cttcatcgtg cggccgactg
     3361 ggctaaatct gtgttctctt cggcggcgct gggtgatcct cgccgtactg cccgcttggt
     3421 taacgtcgcc gcccaattgg caaaatattc tggtaaatca ataaccatct catcagaggg
     3481 tagtgaagcc atgcaggaag gcgcttaccg atttatccgc aatcccaacg tttctgccga
     3541 ggcgatcaga aaggctggcg ccatgcaaac agtcaagttg gctcaggagt ttcccgaact
     3601 gctggccatt gaggacacca cctctttgag ttatcgccac caggtcgccg aagagcttgg
     3661 caagctgggc tctattcagg ataaatcccg cggatggtgg gttcactccg ttctcttgct
     3721 cgaggccacc acattccgca ccgtaggatt actgcatcag gagtggtgga tgcgcccgga
     3781 tgaccctgcc gatgcggatg aaaaggagag tggcaaatgg ctggcagcgg ccgcaactag
     3841 ccggttacgc atgggcagca tgatgagcaa cgtgattgcg gtctgtgacc gcgaagccga
     3901 tattcatgct tatctgcagg acaaactggc gcataacgag cgcttcgtgg tgcgctccaa
     3961 gcacccacgc aaggacgtag agtctgggtt gtatctgtac gaccatctga agaaccaacc
     4021 ggagttgggt ggctatcaga tcagcattcc gcaaaagggc gtggtggata aacgcggtaa
     4081 acgtaaaaat cgaccagccc gcaaggcgag cttgagcctg cgcagtgggc gcatcacgct
     4141 aaaacagggg aatatcacgc tcaacgcggt gctggccgag gagattaacc cgcccaaggg
     4201 tgagaccccg ttgaaatggt tgttgctgac cagcgaaccg gtcgagtcgc tagcccaagc
     4261 cttgcgcgtc atcgacattt atacccatcg ctggcggatc gaggagttcc ataaggcatg
     4321 gaaaaccgga gcaggagccg agaggcaacg catggaggag ccggataatc tggagcggat
     4381 ggtctcgatc ctctcgtttg ttgcggtcag gctgttacag ctcagagaaa gcttcacgct
     4441 gccgcaagca ctcagggcgc aagggctgct aaaggaagcg gaacacgtag aaagccagtc
     4501 cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa
     4561 acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact
     4621 gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc tctgggaagg
     4681 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
     4741 ggggatcaag atctgacccg ggactagttg gtgtggaagt ccatatatcg gtgatcaata
     4801 tagtggttga catgctggct agtcaacatt gagccttttg atcatgcaaa tatattacgg
     4861 tattttacaa tcaaatatca aacttaacta ttgactttat aacttattta ggtggtaaca
     4921 ttcttataaa aaagaaaaaa attactgcaa aacagtacta gcttttaact tgtatcctag
     4981 gttatctatg ctgtctcacc atagagaata ttacctattt cagaatgtat gtccatgatt
     5041 cgccgggtaa atacatataa tacacaaatc tggcttaata aagtctataa tatatctcat
     5101 aaagaagtgc taaattggct agtgctatat atttttaaga aaatttcttt tgactaagtc
     5161 catatcgact ttgtaaaagt tcactttagc atacatatat tacacgagcc agaaattgta
     5221 acttttgcct aaaatcacaa attgcaaaat ttaattgctt gcaaaaggtc acatgcttat
     5281 aatcaacttt tttaaaaatt taaaatactt ttttattttt tatttttaaa cataaatgaa
     5341 ataatttatt tattgtttat gattaccgaa acataaaacc tgctcaagaa aaagaaactg
     5401 ttttgtcctt ggaaaaaaag cactacctag gagcggccaa aatgccgagg ctttcatagc
     5461 ttaaactctt tacagaaaat aggcattata gatcagttcg agttttctta ttcttccttc
     5521 cggttttatc gtcacagttt tacagtaaat aagtatcacc tcttagagtt aactatgaga
     5581 taagcaagta tcatctcatt tcatttacct gaagtcgagt aaacagaaaa tccaattgtt
     5641 gatgaacctc aatgacttag aactatctat cggcagatca tataaagagg atttaggtac
     5701 tcgagaacgc tccgtacatg cagctaaacc tccaagcatc tctaacttgg gtagtgcttt
     5761 ctccaccatt ttctgtgctt gctccttcgt ggcaagtcca gcccataatg cccagaatgt
     5821 agttgcggat tcgtatgacg ttctgtgctt gatttttgtg ttgtagtcaa agaaaaaccc
     5881 cgactcgtca tcccacatat atttggtaat tgatgaggca acgctaatta tcaacatata
     5941 gattgttatc tatctgcatg aacacgaaat ctttacttga cgacttgagg ctgatggtgt
     6001 ttatgcaaag aaaccactgt gtttaatatg tgtcactgtt tgatattact gtcagcgtag
     6061 aagataatag taaaagcggt taataagtgt atttgagata agtgtgataa agtttttaca
     6121 gcgaaaagac gataaataca agaaaatgat tacgaggata cggagagagg tatgtacatg
     6181 tgtatttata tactaagctg ccggcggttg tttgcaagac cgagaaaagg ctagcaagaa
     6241 tcgggtcatt gtagcgtatg cgcctgtgaa cattctcttc aacaagtttg attccattgc
     6301 ggtgaaatgg taaaagtcaa ccccctgcga tgtatatttt cctgtacaat caatcaaaaa
     6361 gccaaatgat ttagcattat ctttacatct tgttatttta cagattttat gtttagatct
     6421 tttatgcttg cttttcaaaa ggcctgcagg caagtgcaca aacaatactt aaataaatac
     6481 tactcagtaa taacctattt cttagcattt ttgacgaaat ttgctatttt gttagagtct
     6541 tttacaccat ttgtctccac acctccgctt acatcaacac caataacgcc atttaatcta
     6601 agcgcatcac caacattttc tggcgtcagt ccaccagcta acataaaatg taagctctgc
     6661 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     6721 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     6781 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     6841 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     6901 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     6961 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7021 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7081 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7141 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7201 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7261 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7321 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7381 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7441 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7501 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7561 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7741 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7801 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7861 atgagtaaac ttggtctgac aggagctcgc tcagaagaac tcgtcaagaa ggcgatagaa
     7921 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     7981 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     8041 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     8101 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatccgcgc
     8161 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     8221 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     8281 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     8341 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     8401 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     8461 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttggagtt cattcagggc
     8521 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     8581 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     8641 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
     8701 tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
     8761 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
     8821 cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca
     8881 agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     8941 attcatccgg gcgatcgatg gaagccggcg gcacctcgct aacggattca ccactccaag
     9001 aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa
     9061 catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatcgggg ggggggtttc
     9121 aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt
     9181 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
     9241 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
     9301 tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
     9361 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
     9421 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
     9481 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
     9541 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
     9601 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
     9661 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
     9721 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
     9781 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
     9841 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
     9901 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
     9961 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat
    10021 ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg
    10081 gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact
    10141 aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
    10201 aattatatca gttattaccc gggaatctcg gtcgtaatga tttttataat gacgaaaaaa
    10261 aaaaaattgg aaagaaaacc ccccccccgc agcgttgggt cctggccacg ggtgcgcatg
    10321 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
    10381 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
    10441 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
    10501 tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt
    10561 ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg
    10621 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
    10681 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc
    10741 atgaacagaa attccccctt acacggaggc atcaagtgac caaacaggaa aaaaccgccc
    10801 ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc
    10861 tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt
    10921 accgcaggtg ggccattctc atgaagaata tcttgaattt attgtcatat ta
//
