LOCUS       Exported               10791 bp DNA     circular SYN 27-SEP-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
COMMENT     SECID/File created by Clone Manager, Scientific
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        104..155
                     /label=P mob
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /note="/SECDescr=mob promoter including oriT (Szpirer et al
                     2001, PMID: 11222611)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P mob"
                     /note="/SECStyleId=1"
     CDS             223..1242
                     /codon_start=1
                     /transl_table=11
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /label=mob
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID: 
                     19928068328)."
                     /note="/SECDescr=Mob protein necessary for mobilization"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=mob"
                     /note="/SECStyleId=1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     misc_feature    1243..1261
                     /label=OE-L
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /note="/SECDescr=outside end sequence of transposon Tn5 
                     (Reznikoff, 2008, PMID:18680433)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=OE-L"
                     /note="/SECStyleId=1"
     misc_feature    1262..1301
                     /label=Core_up
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /note="/SECDescr=upstream sequence of YT_core which can be 
                     extracted with I-PpoI."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=Core_up"
     misc_feature    1302..1316
                     /label=I-PpoI
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-PpoI, enabling extraction of YT_core."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-PpoI"
     misc_feature    1317..1356
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /note="/SECDescr=upstream sequence of promoter slot 
                     (AsiSI/PI-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=P_up"
     misc_feature    1357..1393
                     /label=PI-SceI
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     PI-SceI, enabling e.g. promoter insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=PI-SceI"
     misc_feature    1394..1401
                     /label=AsiSI
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /note="/SECDescr=recognition site of endonuclease AsiSI, 
                     enabling e.g. promoter insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=AsiSI"
     misc_feature    1402..1521
                     /label=CIS_up
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /note="/SECDescr=upstream sequence of Cluster Integration 
                     Site (I-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=CIS_up"
     misc_feature    1402..1441
                     /label=P_dn
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /note="/SECDescr=downstream sequence of promoter slot 
                     (AsiSI/PI-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=P_dn"
     misc_feature    1522..1539
                     /label=I-SceI
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-SceI, enabling target gene insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-SceI"
     misc_feature    1540..1659
                     /label=CIS_dn
                     /note="downstream sequence of Cluster Integration Site 
                     (I-SceI)."
                     /note="/SECDescr=downstream sequence of Cluster Integration
                     Site (I-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=CIS_dn"
     misc_feature    1620..1659
                     /gene="R_up"
                     /label=R_up
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /note="/SECDescr=upstream sequence of reporter slot 
                     (MauBI/I-CeuI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=R_up"
     misc_feature    1660..1667
                     /label=MauBI
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion."
                     /note="/SECDescr=recognition site of endonuclease MauBI, 
                     enabling e.g. reporter insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=MauBI"
     misc_feature    1668..1693
                     /label=I-CeuI
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-CeuI, enabling e.g. reporter insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-CeuI"
     misc_feature    1694..1733
                     /label=R_dn
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /note="/SECDescr=downstream sequence of reporter slot 
                     (MauBI/I-CeuI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=R_dn"
     misc_feature    1733..1790
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /label=MCS Integration site
                     /note="/SECDrawAs=Info only"
     misc_feature    1734..1789
                     /label=MC
                     /note="region with Multiple Cutters 
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon 
                     Tn5 insertion site elucidation."
                     /note="/SECDescr=region with Multiple Cutters 
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon 
                     Tn5 insertion site elucidation."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=MC"
     misc_feature    1790..1829
                     /label=M_up
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /note="/SECDescr=upstream sequence of marker slot 
                     (SalI/PI-PspI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=M_up"
     misc_feature    1830..2129
                     /label=P aadA
                     /note="5'-UTR of aadA gene incl. promoter and RBS as in
                     pHP45Ω ( Prentki and Kirsch 1984, PMID: 6237955)."
                     /note="/SECDescr=5'-UTR of aadA gene incl. promoter and RBS
                     as in pHP45Ω ( Prentki and Kirsch 1984, PMID: 6237955)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P aadA"
                     /note="/SECStyleId=1"
     CDS             2130..2921
                     /codon_start=1
                     /transl_table=11
                     /gene="aadA"
                     /product="aminoglycoside (3'') (9) adenylyltransferase"
                     /label=aadA
                     /note="SmR from pHP45Ω (Prentki and Kirsch 1984, PMID: 
                     6237955)."
                     /note="/SECDescr=aminoglycoside (3'') (9) 
                     adenylyltransferase"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=aadA"
                     /note="/SECStyleId=1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2922..2927
                     /label=SalI
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /note="/SECDescr=recognition site of endonuclease SalI, 
                     enabling e.g. marker insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=SalI"
     misc_feature    2928..2957
                     /label=PI-PspI
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     PI-PspI, enabling e.g. marker insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=PI-PspI"
     misc_feature    2958..2997
                     /label=M_dn
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /note="/SECDescr=downstream sequence of marker slot 
                     (SalI/PI-PspI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=M_dn"
     misc_feature    2998..3012
                     /label=I-PpoI
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-PpoI, enabling extraction of YT_core."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-PpoI"
     misc_feature    3013..3052
                     /label=Core_dn
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /note="/SECDescr=downstream sequence of YT_core which can 
                     be extracted with I-PpoI."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=Core_dn"
     misc_feature    complement(3053..3071)
                     /label=OE-R
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /note="/SECDescr=outside end sequence of transposon Tn5 
                     (Reznikoff, 2008, PMID:18680433)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=OE-R"
                     /note="/SECStyleId=1"
     promoter        3082..3112
                     /label=P tnp
                     /note="transposon Tn5 transposase promoter (Yin et al
                     1988, PMID: 2451025)."
                     /note="/SECDescr=transposon Tn5 transposase promoter (Yin 
                     et al 1988, PMID: 2451025)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P tnp"
                     /note="/SECStyleId=1"
     CDS             3145..4575
                     /codon_start=1
                     /transl_table=11
                     /gene="tnp"
                     /product="transposon Tn5 transposase"
                     /label=tnp
                     /note="transposon Tn5 transposase (Goryshin & Reznikoff
                     1998, PMID: 9516433)."
                     /note="/SECDescr=transposon Tn5 transposase"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=tnp"
                     /note="/SECStyleId=1"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     misc_feature    5087..5197
                     /label=CEN4
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /note="/SECDescr=CEN4 of Saccharomyces cerevisiae Chr IV 
                     (Saccharomyces Genome Database)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=CEN4"
                     /note="/SECStyleId=1"
     misc_feature    5729..6473
                     /label=ARS1
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /note="/SECDescr=ARS1 of yCP50 (Genbank: X70276.1)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=ARS1 "
                     /note="/SECStyleId=1"
     rep_origin      6943..7531
                     /label=pMB1 ori
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); 
                     annotated as New England Biolabs (2007)."
                     /note="/SECDescr=origin of replication from plasmid pBR322 
                     (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 
                     383387); annotated as New England Biolabs (2007)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=pMB1 ori"
                     /note="/SECStyleId=1"
     CDS             complement(7710..8504)
                     /codon_start=1
                     /transl_table=11
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /label=aphII
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin 
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /note="/SECDescr=aminoglycoside 3'-phosphotransferase"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=aphII"
                     /note="/SECStyleId=1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        8594..8642
                     /label=P aphII
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank 
                     U00004.1)."
                     /note="/SECDescr=promoter of aphII gene in Transposon Tn5 
                     (Genbank U00004.1)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P aphII"
                     /note="/SECStyleId=1"
     promoter        8942..9157
                     /label=P URA3
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /note="/SECDescr=URA3 promoter region (Roy et al 1990, 
                     PMID: 2204810)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P URA3"
                     /note="/SECStyleId=1"
     CDS             9158..9961
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /label=URA3
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /note="/SECDescr=orotidine 5'-phosphate decarboxylase"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=URA3"
                     /note="/SECStyleId=1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgtaa ctataacggt
     1681 cctaaggtag cgagcttact tgtgctgttg tgtaaggaca cgcttaaccc agagaattcc
     1741 agcgacctat tctacgacta cgcgtccatg gctcgaggag ctcggtaccg ccccactacc
     1801 cgatttatca agatttggcg gagagtcccc gcacaccgtg gaaacggatg aaggcacgaa
     1861 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     1921 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     1981 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     2041 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     2101 gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga
     2161 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2221 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     2281 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     2341 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     2401 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     2461 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     2521 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     2581 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     2641 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     2701 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     2761 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     2821 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     2881 actacgtgaa aggcgagatc accaaggtag tcggcaaata agtcgactgg caaacagcta
     2941 ttatgggtat tatgggtgtg tatccagaac gacggatctt gacgcaattt ggcgtggctc
     3001 tcttaaggta gccctccgac caattgaggt gcgctctgac cgatcagttt agacttgtgt
     3061 ataagagtca gagcgtcctg aacggaacct ttcccgtttt ccaggatctg atcttccatg
     3121 tgacctccta acatggtaac gttcatgata acttctgctc ttcatcgtgc ggccgactgg
     3181 gctaaatctg tgttctcttc ggcggcgctg ggtgatcctc gccgtactgc ccgcttggtt
     3241 aacgtcgccg cccaattggc aaaatattct ggtaaatcaa taaccatctc atcagagggt
     3301 agtgaagcca tgcaggaagg cgcttaccga tttatccgca atcccaacgt ttctgccgag
     3361 gcgatcagaa aggctggcgc catgcaaaca gtcaagttgg ctcaggagtt tcccgaactg
     3421 ctggccattg aggacaccac ctctttgagt tatcgccacc aggtcgccga agagcttggc
     3481 aagctgggct ctattcagga taaatcccgc ggatggtggg ttcactccgt tctcttgctc
     3541 gaggccacca cattccgcac cgtaggatta ctgcatcagg agtggtggat gcgcccggat
     3601 gaccctgccg atgcggatga aaaggagagt ggcaaatggc tggcagcggc cgcaactagc
     3661 cggttacgca tgggcagcat gatgagcaac gtgattgcgg tctgtgaccg cgaagccgat
     3721 attcatgctt atctgcagga caaactggcg cataacgagc gcttcgtggt gcgctccaag
     3781 cacccacgca aggacgtaga gtctgggttg tatctgtacg accatctgaa gaaccaaccg
     3841 gagttgggtg gctatcagat cagcattccg caaaagggcg tggtggataa acgcggtaaa
     3901 cgtaaaaatc gaccagcccg caaggcgagc ttgagcctgc gcagtgggcg catcacgcta
     3961 aaacagggga atatcacgct caacgcggtg ctggccgagg agattaaccc gcccaagggt
     4021 gagaccccgt tgaaatggtt gttgctgacc agcgaaccgg tcgagtcgct agcccaagcc
     4081 ttgcgcgtca tcgacattta tacccatcgc tggcggatcg aggagttcca taaggcatgg
     4141 aaaaccggag caggagccga gaggcaacgc atggaggagc cggataatct ggagcggatg
     4201 gtctcgatcc tctcgtttgt tgcggtcagg ctgttacagc tcagagaaag cttcacgctg
     4261 ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc
     4321 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
     4381 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
     4441 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctgggaaggt
     4501 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag
     4561 gggatcaaga tctgacccgg gactagttgg tgtggaagtc catatatcgg tgatcaatat
     4621 agtggttgac atgctggcta gtcaacattg agccttttga tcatgcaaat atattacggt
     4681 attttacaat caaatatcaa acttaactat tgactttata acttatttag gtggtaacat
     4741 tcttataaaa aagaaaaaaa ttactgcaaa acagtactag cttttaactt gtatcctagg
     4801 ttatctatgc tgtctcacca tagagaatat tacctatttc agaatgtatg tccatgattc
     4861 gccgggtaaa tacatataat acacaaatct ggcttaataa agtctataat atatctcata
     4921 aagaagtgct aaattggcta gtgctatata tttttaagaa aatttctttt gactaagtcc
     4981 atatcgactt tgtaaaagtt cactttagca tacatatatt acacgagcca gaaattgtaa
     5041 cttttgccta aaatcacaaa ttgcaaaatt taattgcttg caaaaggtca catgcttata
     5101 atcaactttt ttaaaaattt aaaatacttt tttatttttt atttttaaac ataaatgaaa
     5161 taatttattt attgtttatg attaccgaaa cataaaacct gctcaagaaa aagaaactgt
     5221 tttgtccttg gaaaaaaagc actacctagg agcggccaaa atgccgaggc tttcatagct
     5281 taaactcttt acagaaaata ggcattatag atcagttcga gttttcttat tcttccttcc
     5341 ggttttatcg tcacagtttt acagtaaata agtatcacct cttagagtta actatgagat
     5401 aagcaagtat catctcattt catttacctg aagtcgagta aacagaaaat ccaattgttg
     5461 atgaacctca atgacttaga actatctatc ggcagatcat ataaagagga tttaggtact
     5521 cgagaacgct ccgtacatgc agctaaacct ccaagcatct ctaacttggg tagtgctttc
     5581 tccaccattt tctgtgcttg ctccttcgtg gcaagtccag cccataatgc ccagaatgta
     5641 gttgcggatt cgtatgacgt tctgtgcttg atttttgtgt tgtagtcaaa gaaaaacccc
     5701 gactcgtcat cccacatata tttggtaatt gatgaggcaa cgctaattat caacatatag
     5761 attgttatct atctgcatga acacgaaatc tttacttgac gacttgaggc tgatggtgtt
     5821 tatgcaaaga aaccactgtg tttaatatgt gtcactgttt gatattactg tcagcgtaga
     5881 agataatagt aaaagcggtt aataagtgta tttgagataa gtgtgataaa gtttttacag
     5941 cgaaaagacg ataaatacaa gaaaatgatt acgaggatac ggagagaggt atgtacatgt
     6001 gtatttatat actaagctgc cggcggttgt ttgcaagacc gagaaaaggc tagcaagaat
     6061 cgggtcattg tagcgtatgc gcctgtgaac attctcttca acaagtttga ttccattgcg
     6121 gtgaaatggt aaaagtcaac cccctgcgat gtatattttc ctgtacaatc aatcaaaaag
     6181 ccaaatgatt tagcattatc tttacatctt gttattttac agattttatg tttagatctt
     6241 ttatgcttgc ttttcaaaag gcctgcaggc aagtgcacaa acaatactta aataaatact
     6301 actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt
     6361 ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa
     6421 gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctctgcc
     6481 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     6541 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     6601 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     6661 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     6721 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     6781 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6841 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6901 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6961 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7021 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7081 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7141 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7201 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7261 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7321 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7381 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7441 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7501 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7561 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7621 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7681 tgagtaaact tggtctgaca ggagctcgct cagaagaact cgtcaagaag gcgatagaag
     7741 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     7801 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     7861 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     7921 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catccgcgcc
     7981 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     8041 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     8101 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     8161 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     8221 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     8281 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca
     8341 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     8401 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     8461 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     8521 cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     8581 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     8641 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
     8701 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     8761 ttcatccggg cgatcgatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     8821 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     8881 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatcggggg gggggtttca
     8941 attcaattca tcattttttt tttattcttt tttttgattt cggtttcttt gaaatttttt
     9001 tgattcggta atctccgaac agaaggaaga acgaaggaag gagcacagac ttagattggt
     9061 atatatacgc atatgtagtg ttgaagaaac atgaaattgc ccagtattct taacccaact
     9121 gcacagaaca aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta catataagga
     9181 acgtgctgct actcatccta gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa
     9241 gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc aaggaattac tggagttagt
     9301 tgaagcatta ggtcccaaaa tttgtttact aaaaacacat gtggatatct tgactgattt
     9361 ttccatggag ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact
     9421 cttcgaagac agaaaatttg ctgacattgg taatacagtc aaattgcagt actctgcggg
     9481 tgtatacaga atagcagaat gggcagacat tacgaatgca cacggtgtgg tgggcccagg
     9541 tattgttagc ggtttgaagc aggcggcaga agaagtaaca aaggaaccta gaggcctttt
     9601 gatgttagca gaattgtcat gcaagggctc cctatctact ggagaatata ctaagggtac
     9661 tgttgacatt gcgaagagcg acaaagattt tgttatcggc tttattgctc aaagagacat
     9721 gggtggaaga gatgaaggtt acgattggtt gattatgaca cccggtgtgg gtttagatga
     9781 caagggagac gcattgggtc aacagtatag aaccgtggat gatgtggtct ctacaggatc
     9841 tgacattatt attgttggaa gaggactatt tgcaaaggga agggatgcta aggtagaggg
     9901 tgaacgttac agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta
     9961 aaaaactgta ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta
    10021 attatatcag ttattacccg ggaatctcgg tcgtaatgat ttttataatg acgaaaaaaa
    10081 aaaaattgga aagaaaaccc cccccccgca gcgttgggtc ctggccacgg gtgcgcatga
    10141 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
    10201 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
    10261 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
    10321 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
    10381 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt
    10441 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
    10501 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca
    10561 tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct
    10621 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
    10681 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
    10741 ccgcaggtgg gccattctca tgaagaatat cttgaattta ttgtcatatt a
//
