LOCUS       pYTRW15K_2G5           11188 bp    DNA     circular SYN 27-SEP-2021
DEFINITION  
ACCESSION   pYTRW15K_2G5
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     promoter        104..155
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /label=P mob
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P mob"
                     /SECDescr="mob promoter including oriT (Szpirer et al 2001, PMID: 11222611)."
     CDS             223..1242
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID:
                     19928068328)."
                     /transl_table=11
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mob"
                     /SECDescr="Mob protein necessary for mobilization"
     misc_feature    1243..1261
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-L
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-L"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     misc_feature    1262..1301
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /label=Core_up
                     /SECDrawAs="Label"
                     /SECName="Core_up"
                     /SECDescr="upstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    1302..1316
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    1317..1356
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /SECDrawAs="Label"
                     /SECName="P_up"
                     /SECDescr="upstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1357..1393
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /label=PI-SceI
                     /SECDrawAs="Label"
                     /SECName="PI-SceI"
                     /SECDescr="recognition site of homing endonuclease PI-SceI, enabling e.g. promoter insertion."
     misc_feature    1394..1401
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /label=AsiSI
                     /SECDrawAs="Label"
                     /SECName="AsiSI"
                     /SECDescr="recognition site of endonuclease AsiSI, enabling e.g. promoter insertion."
     misc_feature    1402..1441
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /label=P_dn
                     /SECDrawAs="Label"
                     /SECName="P_dn"
                     /SECDescr="downstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1402..1521
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_up
                     /SECDrawAs="Label"
                     /SECName="CIS_up"
                     /SECDescr="upstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1522..1539
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /label=I-SceI
                     /SECDrawAs="Label"
                     /SECName="I-SceI"
                     /SECDescr="recognition site of homing endonuclease I-SceI, enabling target gene insertion."
     misc_feature    1540..1659
                     /note="downstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_dn
                     /SECDrawAs="Label"
                     /SECName="CIS_dn"
                     /SECDescr="downstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1620..1659
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /gene="R_up"
                     /SECDrawAs="Label"
                     /SECName="R_up"
                     /SECDescr="upstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    1660..1667
                     /label=MauBI
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion/exchange."
     CDS             1689..2396
                     /gene="mTagBFP2"
                     /product="blue fluorescent protein mTagBFP2"
                     /transl_table=11
                     /note="blue fluorescent protein from Entacmaea quadricolor
                     (Subach et al., 2011; PMID: 22174863)."
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVL
                     TATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLNLE"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mTagBFP2"
                     /SECDescr="blue fluorescent protein mTagBFP2"
     misc_feature    2397..2404
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /label=MauBI
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion/exchange."
     misc_feature    2405..2430
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion/exchange."
                     /label=I-CeuI
                     /SECDrawAs="Label"
                     /SECName="I-CeuI"
                     /SECDescr="recognition site of homing endonuclease I-CeuI, enabling e.g. reporter insertion/exchange."
     misc_feature    2431..2470
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /label=R_dn
                     /SECDrawAs="Label"
                     /SECName="R_dn"
                     /SECDescr="downstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    2470..2527
                     /label=MCS Integration site
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /SECDrawAs="Info only"
     misc_feature    2471..2526
                     /note="region with Multiple Cutters
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon
                     Tn5 insertion site elucidation."
                     /label=MC
                     /SECDrawAs="Label"
                     /SECName="MC"
                     /SECDescr="region with Multiple Cutters EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon Tn5 insertion site elucidation."
     misc_feature    2527..2566
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_up
                     /SECDrawAs="Label"
                     /SECName="M_up"
                     /SECDescr="upstream sequence of marker slot (SalI/PI-PspI)."
     mobile_element  complement(2567..3318)
                     /mobile_element_type="transposon:mini-Tn7"
                     /SECDrawAs="Info only"
                     /SECName="'mobile_element'"
     source          complement(2567..3318)
                     /organism="Cloning vector pUC18-mini-Tn7-Gm"
                     /mol_type="other DNA"
                     /db_xref="taxon:295022"
                     /SECDrawAs="Info only"
                     /SECName="'source'"
     promoter        2568..2597
                     /note="aacC1 promoter region (Wohlleben et al 1989, PMID:
                     2549372)."
                     /label=P aacC1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aacC1"
                     /SECDescr="aacC1 promoter region (Wohlleben et al 1989, PMID: 2549372)."
     CDS             2785..3318
                     /gene="aacC1"
                     /transl_table=11
                     /product="gentamicin acetyl transferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL
                     DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQP
                     RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL
                     GIREEVMHFDIDPSTAT"
                     /note="aacC1 from pUCGM (Schweizer 1993, PMID: 8267974)."
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aacC1"
                     /SECDescr="gentamicin acetyl transferase"
     gene            2785..3318
                     /gene="aacC1"
                     /SECDrawAs="Info only"
                     /SECName="aacC1"
     misc_feature    3319..3324
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /label=SalI
                     /SECDrawAs="Label"
                     /SECName="SalI"
                     /SECDescr="recognition site of endonuclease SalI, enabling e.g. marker insertion."
     misc_feature    3325..3354
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /label=PI-PspI
                     /SECDrawAs="Label"
                     /SECName="PI-PspI"
                     /SECDescr="recognition site of homing endonuclease PI-PspI, enabling e.g. marker insertion."
     misc_feature    3355..3394
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_dn
                     /SECDrawAs="Label"
                     /SECName="M_dn"
                     /SECDescr="downstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    3395..3409
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    3410..3449
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /label=Core_dn
                     /SECDrawAs="Label"
                     /SECName="Core_dn"
                     /SECDescr="downstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    complement(3450..3468)
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-R
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-R"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     promoter        3479..3509
                     /note="transposon Tn5 transposase promoter (Yin et al
                     1988, PMID: 2451025)."
                     /label=P tnp
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tnp"
                     /SECDescr="transposon Tn5 transposase promoter (Yin et al 1988, PMID: 2451025)."
     CDS             3542..4972
                     /gene="tnp"
                     /product="transposon Tn5 transposase"
                     /transl_table=11
                     /note="transposon Tn5 transposase (Goryshin & Reznikoff
                     1998, PMID: 9516433)."
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGK
                     SITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLS
                     YRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEK
                     ESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDV
                     ESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGN
                     ITLNAVLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKT
                     GAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQS
                     AETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW
                     EGWEALQSKLDGFLAAKDLMAQGIKI"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tnp"
                     /SECDescr="transposon Tn5 transposase"
     misc_feature    5484..5594
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /label=CEN4
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="CEN4"
                     /SECDescr="CEN4 of Saccharomyces cerevisiae Chr IV (Saccharomyces Genome Database)."
     misc_feature    6126..6870
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /label=ARS1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ARS1 "
                     /SECDescr="ARS1 of yCP50 (Genbank: X70276.1)."
     rep_origin      7340..7928
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387);
                     annotated as New England Biolabs (2007)."
                     /label=pMB1 ori
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pMB1 ori"
                     /SECDescr="origin of replication from plasmid pBR322 (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); annotated as New England Biolabs (2007)."
     CDS             complement(8107..8901)
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /transl_table=11
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aphII"
                     /SECDescr="aminoglycoside 3'-phosphotransferase"
     promoter        8991..9039
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank
                     U00004.1)."
                     /label=P aphII
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aphII"
                     /SECDescr="promoter of aphII gene in Transposon Tn5 (Genbank U00004.1)."
     promoter        9339..9554
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /label=P URA3
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P URA3"
                     /SECDescr="URA3 promoter region (Roy et al 1990, PMID: 2204810)."
     CDS             9555..10358
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="URA3"
                     /SECDescr="orotidine 5'-phosphate decarboxylase"
ORIGIN      
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgaac tttagaagga
     1681 gatataccat gagcgagctg attaaggaga acatgcacat gaagctgtac atggagggca
     1741 ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     1801 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     1861 tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg
     1921 acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag
     1981 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     2041 acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac
     2101 tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa
     2161 acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc aagaccacat
     2221 atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca
     2281 gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag
     2341 tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaatctc gagtaacgcg
     2401 cgcgtaacta taacggtcct aaggtagcga gcttacttgt gctgttgtgt aaggacacgc
     2461 ttaacccaga gaattccagc gacctattct acgactacgc gtccatggct cgaggagctc
     2521 ggtaccgccc cactacccga tttatcaaga tttggcggag agtcccattg acataagcct
     2581 gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2641 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2701 tttttgtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     2761 gtttgatgtt atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc
     2821 agtcgcccta aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc
     2881 ggccctgacc aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga
     2941 gacgtagcca cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt
     3001 agtaagacat tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg
     3061 gcttacgttc tgcccaagtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca
     3121 gtctccggcg agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat
     3181 gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc
     3241 gcagtggctc tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac
     3301 ccaagtaccg ccacctaagt cgactggcaa acagctatta tgggtattat gggtgtgtat
     3361 ccagaacgac ggatcttgac gcaatttggc gtggctctct taaggtagcc ctccgaccaa
     3421 ttgaggtgcg ctctgaccga tcagtttaga cttgtgtata agagtcagag cgtcctgaac
     3481 ggaacctttc ccgttttcca ggatctgatc ttccatgtga cctcctaaca tggtaacgtt
     3541 catgataact tctgctcttc atcgtgcggc cgactgggct aaatctgtgt tctcttcggc
     3601 ggcgctgggt gatcctcgcc gtactgcccg cttggttaac gtcgccgccc aattggcaaa
     3661 atattctggt aaatcaataa ccatctcatc agagggtagt gaagccatgc aggaaggcgc
     3721 ttaccgattt atccgcaatc ccaacgtttc tgccgaggcg atcagaaagg ctggcgccat
     3781 gcaaacagtc aagttggctc aggagtttcc cgaactgctg gccattgagg acaccacctc
     3841 tttgagttat cgccaccagg tcgccgaaga gcttggcaag ctgggctcta ttcaggataa
     3901 atcccgcgga tggtgggttc actccgttct cttgctcgag gccaccacat tccgcaccgt
     3961 aggattactg catcaggagt ggtggatgcg cccggatgac cctgccgatg cggatgaaaa
     4021 ggagagtggc aaatggctgg cagcggccgc aactagccgg ttacgcatgg gcagcatgat
     4081 gagcaacgtg attgcggtct gtgaccgcga agccgatatt catgcttatc tgcaggacaa
     4141 actggcgcat aacgagcgct tcgtggtgcg ctccaagcac ccacgcaagg acgtagagtc
     4201 tgggttgtat ctgtacgacc atctgaagaa ccaaccggag ttgggtggct atcagatcag
     4261 cattccgcaa aagggcgtgg tggataaacg cggtaaacgt aaaaatcgac cagcccgcaa
     4321 ggcgagcttg agcctgcgca gtgggcgcat cacgctaaaa caggggaata tcacgctcaa
     4381 cgcggtgctg gccgaggaga ttaacccgcc caagggtgag accccgttga aatggttgtt
     4441 gctgaccagc gaaccggtcg agtcgctagc ccaagccttg cgcgtcatcg acatttatac
     4501 ccatcgctgg cggatcgagg agttccataa ggcatggaaa accggagcag gagccgagag
     4561 gcaacgcatg gaggagccgg ataatctgga gcggatggtc tcgatcctct cgtttgttgc
     4621 ggtcaggctg ttacagctca gagaaagctt cacgctgccg caagcactca gggcgcaagg
     4681 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     4741 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     4801 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     4861 aaccggaatt gccagctggg gcgccctctg ggaaggttgg gaagccctgc aaagtaaact
     4921 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gacccgggac
     4981 tagttggtgt ggaagtccat atatcggtga tcaatatagt ggttgacatg ctggctagtc
     5041 aacattgagc cttttgatca tgcaaatata ttacggtatt ttacaatcaa atatcaaact
     5101 taactattga ctttataact tatttaggtg gtaacattct tataaaaaag aaaaaaatta
     5161 ctgcaaaaca gtactagctt ttaacttgta tcctaggtta tctatgctgt ctcaccatag
     5221 agaatattac ctatttcaga atgtatgtcc atgattcgcc gggtaaatac atataataca
     5281 caaatctggc ttaataaagt ctataatata tctcataaag aagtgctaaa ttggctagtg
     5341 ctatatattt ttaagaaaat ttcttttgac taagtccata tcgactttgt aaaagttcac
     5401 tttagcatac atatattaca cgagccagaa attgtaactt ttgcctaaaa tcacaaattg
     5461 caaaatttaa ttgcttgcaa aaggtcacat gcttataatc aactttttta aaaatttaaa
     5521 atactttttt attttttatt tttaaacata aatgaaataa tttatttatt gtttatgatt
     5581 accgaaacat aaaacctgct caagaaaaag aaactgtttt gtccttggaa aaaaagcact
     5641 acctaggagc ggccaaaatg ccgaggcttt catagcttaa actctttaca gaaaataggc
     5701 attatagatc agttcgagtt ttcttattct tccttccggt tttatcgtca cagttttaca
     5761 gtaaataagt atcacctctt agagttaact atgagataag caagtatcat ctcatttcat
     5821 ttacctgaag tcgagtaaac agaaaatcca attgttgatg aacctcaatg acttagaact
     5881 atctatcggc agatcatata aagaggattt aggtactcga gaacgctccg tacatgcagc
     5941 taaacctcca agcatctcta acttgggtag tgctttctcc accattttct gtgcttgctc
     6001 cttcgtggca agtccagccc ataatgccca gaatgtagtt gcggattcgt atgacgttct
     6061 gtgcttgatt tttgtgttgt agtcaaagaa aaaccccgac tcgtcatccc acatatattt
     6121 ggtaattgat gaggcaacgc taattatcaa catatagatt gttatctatc tgcatgaaca
     6181 cgaaatcttt acttgacgac ttgaggctga tggtgtttat gcaaagaaac cactgtgttt
     6241 aatatgtgtc actgtttgat attactgtca gcgtagaaga taatagtaaa agcggttaat
     6301 aagtgtattt gagataagtg tgataaagtt tttacagcga aaagacgata aatacaagaa
     6361 aatgattacg aggatacgga gagaggtatg tacatgtgta tttatatact aagctgccgg
     6421 cggttgtttg caagaccgag aaaaggctag caagaatcgg gtcattgtag cgtatgcgcc
     6481 tgtgaacatt ctcttcaaca agtttgattc cattgcggtg aaatggtaaa agtcaacccc
     6541 ctgcgatgta tattttcctg tacaatcaat caaaaagcca aatgatttag cattatcttt
     6601 acatcttgtt attttacaga ttttatgttt agatctttta tgcttgcttt tcaaaaggcc
     6661 tgcaggcaag tgcacaaaca atacttaaat aaatactact cagtaataac ctatttctta
     6721 gcatttttga cgaaatttgc tattttgtta gagtctttta caccatttgt ctccacacct
     6781 ccgcttacat caacaccaat aacgccattt aatctaagcg catcaccaac attttctggc
     6841 gtcagtccac cagctaacat aaaatgtaag ctctgcctcg cgcgtttcgg tgatgacggt
     6901 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     6961 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     7021 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     7081 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     7141 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7201 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7261 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7321 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7381 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7441 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7501 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7561 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7621 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7681 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7741 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     7801 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7861 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7921 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7981 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8041 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagga
     8101 gctcgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     8161 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     8221 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     8281 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     8341 ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg agcctggcga acagttcggc
     8401 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     8461 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     8521 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     8581 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     8641 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     8701 tagccgcgct gcctcgtctt ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     8761 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     8821 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     8881 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcttga
     8941 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     9001 cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac
     9061 cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct
     9121 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccgggcga tcgatggaag
     9181 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg
     9241 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc
     9301 cagcagccgc acgcggcgca tcgggggggg ggtttcaatt caattcatca tttttttttt
     9361 attctttttt ttgatttcgg tttctttgaa atttttttga ttcggtaatc tccgaacaga
     9421 aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg
     9481 aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga
     9541 aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc
     9601 ctgttgctgc caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat
     9661 tggatgttcg taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt
     9721 gtttactaaa aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc
     9781 cgctaaaggc attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg
     9841 acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg
     9901 cagacattac gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg
     9961 cggcagaaga agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca
    10021 agggctccct atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca
    10081 aagattttgt tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg
    10141 attggttgat tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac
    10201 agtatagaac cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag
    10261 gactatttgc aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct
    10321 gggaagcata tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg
    10381 catgtatact aaactcacaa attagagctt caatttaatt atatcagtta ttacccggga
    10441 atctcggtcg taatgatttt tataatgacg aaaaaaaaaa aattggaaag aaaacccccc
    10501 ccccgcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
    10561 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
    10621 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
    10681 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
    10741 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
    10801 aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
    10861 tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg
    10921 agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaattc ccccttacac
    10981 ggaggcatca agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa
    11041 gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca
    11101 tctgtgaatc gcttcacgac cacgctgatg agctttaccg caggtgggcc attctcatga
    11161 agaatatctt gaatttattg tcatatta
//
