LOCUS       Exported               11200 bp DNA     circular SYN 27-SEP-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
COMMENT     SECID/File created by Clone Manager, Scientific
FEATURES             Location/Qualifiers
     source          1..11200
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2579..3330
                     /organism="Cloning vector pUC18-mini-Tn7-Gm"
                     /mol_type="other DNA"
                     /note="/SECDrawAs=Info only"
                     /note="/SECName='source'"
                     /db_xref="taxon:295022"
     promoter        104..155
                     /label=P mob
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /note="/SECDescr=mob promoter including oriT (Szpirer et al
                     2001, PMID: 11222611)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P mob"
                     /note="/SECStyleId=1"
     CDS             223..1242
                     /codon_start=1
                     /transl_table=11
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /label=mob
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID: 
                     19928068328)."
                     /note="/SECDescr=Mob protein necessary for mobilization"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=mob"
                     /note="/SECStyleId=1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     misc_feature    1243..1261
                     /label=OE-L
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /note="/SECDescr=outside end sequence of transposon Tn5 
                     (Reznikoff, 2008, PMID:18680433)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=OE-L"
                     /note="/SECStyleId=1"
     misc_feature    1262..1301
                     /label=Core_up
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /note="/SECDescr=upstream sequence of YT_core which can be 
                     extracted with I-PpoI."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=Core_up"
     misc_feature    1302..1316
                     /label=I-PpoI
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-PpoI, enabling extraction of YT_core."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-PpoI"
     misc_feature    1317..1356
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /note="/SECDescr=upstream sequence of promoter slot 
                     (AsiSI/PI-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=P_up"
     misc_feature    1357..1393
                     /label=PI-SceI
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     PI-SceI, enabling e.g. promoter insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=PI-SceI"
     misc_feature    1394..1401
                     /label=AsiSI
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /note="/SECDescr=recognition site of endonuclease AsiSI, 
                     enabling e.g. promoter insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=AsiSI"
     misc_feature    1402..1521
                     /label=CIS_up
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /note="/SECDescr=upstream sequence of Cluster Integration 
                     Site (I-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=CIS_up"
     misc_feature    1402..1441
                     /label=P_dn
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /note="/SECDescr=downstream sequence of promoter slot 
                     (AsiSI/PI-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=P_dn"
     misc_feature    1522..1539
                     /label=I-SceI
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-SceI, enabling target gene insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-SceI"
     misc_feature    1540..1659
                     /label=CIS_dn
                     /note="downstream sequence of Cluster Integration Site 
                     (I-SceI)."
                     /note="/SECDescr=downstream sequence of Cluster Integration
                     Site (I-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=CIS_dn"
     misc_feature    1620..1659
                     /gene="R_up"
                     /label=R_up
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /note="/SECDescr=upstream sequence of reporter slot 
                     (MauBI/I-CeuI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=R_up"
     misc_feature    1660..1667
                     /label=MauBI
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /note="/SECDescr=recognition site of endonuclease MauBI, 
                     enabling e.g. reporter insertion/exchange."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=MauBI"
     CDS             1689..2408
                     /codon_start=1
                     /transl_table=11
                     /gene="eYFP"
                     /product="yellow fluorescent protein eYFP"
                     /label=eYFP
                     /note="yellow fluorescent protein from Aequorea victoria
                     (Ormo et al. 1996, PMID: 8703075; Spiess et al. 2005, 
                     PMID:15725123)."
                     /note="/SECDescr=yellow fluorescent protein eYFP"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=eYFP"
                     /note="/SECStyleId=1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    2409..2416
                     /label=MauBI
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /note="/SECDescr=recognition site of endonuclease MauBI, 
                     enabling e.g. reporter insertion/exchange."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=MauBI"
     misc_feature    2417..2442
                     /label=I-CeuI
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion/exchange."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-CeuI, enabling e.g. reporter insertion/exchange."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-CeuI"
     misc_feature    2443..2482
                     /label=R_dn
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /note="/SECDescr=downstream sequence of reporter slot 
                     (MauBI/I-CeuI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=R_dn"
     misc_feature    2482..2539
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /label=MCS Integration site
                     /note="/SECDrawAs=Info only"
     misc_feature    2483..2538
                     /label=MC
                     /note="region with Multiple Cutters 
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon 
                     Tn5 insertion site elucidation."
                     /note="/SECDescr=region with Multiple Cutters 
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon 
                     Tn5 insertion site elucidation."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=MC"
     misc_feature    2539..2578
                     /label=M_up
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /note="/SECDescr=upstream sequence of marker slot 
                     (SalI/PI-PspI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=M_up"
     mobile_element  complement(2579..3330)
                     /mobile_element_type="transposon:mini-Tn7"
                     /label=Tn7
                     /note="/SECDrawAs=Info only"
                     /note="/SECName='mobile_element'"
     promoter        2580..2609
                     /label=P aacC1
                     /note="aacC1 promoter region (Wohlleben et al 1989, PMID: 
                     2549372)."
                     /note="/SECDescr=aacC1 promoter region (Wohlleben et al 
                     1989, PMID: 2549372)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P aacC1"
                     /note="/SECStyleId=1"
     CDS             2797..3330
                     /codon_start=1
                     /transl_table=11
                     /gene="aacC1"
                     /product="gentamicin acetyl transferase"
                     /label=aacC1
                     /note="aacC1 from pUCGM (Schweizer 1993, PMID: 8267974)."
                     /note="/SECDescr=gentamicin acetyl transferase"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=aacC1"
                     /note="/SECStyleId=1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            2797..3330
                     /gene="aacC1"
                     /label=aacC1
                     /note="/SECDrawAs=Info only"
                     /note="/SECName=aacC1"
     misc_feature    3331..3336
                     /label=SalI
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /note="/SECDescr=recognition site of endonuclease SalI, 
                     enabling e.g. marker insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=SalI"
     misc_feature    3337..3366
                     /label=PI-PspI
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     PI-PspI, enabling e.g. marker insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=PI-PspI"
     misc_feature    3367..3406
                     /label=M_dn
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /note="/SECDescr=downstream sequence of marker slot 
                     (SalI/PI-PspI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=M_dn"
     misc_feature    3407..3421
                     /label=I-PpoI
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-PpoI, enabling extraction of YT_core."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-PpoI"
     misc_feature    3422..3461
                     /label=Core_dn
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /note="/SECDescr=downstream sequence of YT_core which can 
                     be extracted with I-PpoI."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=Core_dn"
     misc_feature    complement(3462..3480)
                     /label=OE-R
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /note="/SECDescr=outside end sequence of transposon Tn5 
                     (Reznikoff, 2008, PMID:18680433)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=OE-R"
                     /note="/SECStyleId=1"
     promoter        3491..3521
                     /label=P tnp
                     /note="transposon Tn5 transposase promoter (Yin et al
                     1988, PMID: 2451025)."
                     /note="/SECDescr=transposon Tn5 transposase promoter (Yin 
                     et al 1988, PMID: 2451025)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P tnp"
                     /note="/SECStyleId=1"
     CDS             3554..4984
                     /codon_start=1
                     /transl_table=11
                     /gene="tnp"
                     /product="transposon Tn5 transposase"
                     /label=tnp
                     /note="transposon Tn5 transposase (Goryshin & Reznikoff
                     1998, PMID: 9516433)."
                     /note="/SECDescr=transposon Tn5 transposase"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=tnp"
                     /note="/SECStyleId=1"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     misc_feature    5496..5606
                     /label=CEN4
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /note="/SECDescr=CEN4 of Saccharomyces cerevisiae Chr IV 
                     (Saccharomyces Genome Database)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=CEN4"
                     /note="/SECStyleId=1"
     misc_feature    6138..6882
                     /label=ARS1
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /note="/SECDescr=ARS1 of yCP50 (Genbank: X70276.1)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=ARS1 "
                     /note="/SECStyleId=1"
     rep_origin      7352..7940
                     /label=pMB1 ori
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); 
                     annotated as New England Biolabs (2007)."
                     /note="/SECDescr=origin of replication from plasmid pBR322 
                     (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 
                     383387); annotated as New England Biolabs (2007)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=pMB1 ori"
                     /note="/SECStyleId=1"
     CDS             complement(8119..8913)
                     /codon_start=1
                     /transl_table=11
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /label=aphII
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin 
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /note="/SECDescr=aminoglycoside 3'-phosphotransferase"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=aphII"
                     /note="/SECStyleId=1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        9003..9051
                     /label=P aphII
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank 
                     U00004.1)."
                     /note="/SECDescr=promoter of aphII gene in Transposon Tn5 
                     (Genbank U00004.1)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P aphII"
                     /note="/SECStyleId=1"
     promoter        9351..9566
                     /label=P URA3
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /note="/SECDescr=URA3 promoter region (Roy et al 1990, 
                     PMID: 2204810)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P URA3"
                     /note="/SECStyleId=1"
     CDS             9567..10370
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /label=URA3
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /note="/SECDescr=orotidine 5'-phosphate decarboxylase"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=URA3"
                     /note="/SECStyleId=1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgaac tttagaagga
     1681 gatataccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1741 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1801 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1861 ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc
     1921 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1981 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2041 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2101 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2161 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2221 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2281 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2341 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2401 acaagtaacg cgcgcgtaac tataacggtc ctaaggtagc gagcttactt gtgctgttgt
     2461 gtaaggacac gcttaaccca gagaattcca gcgacctatt ctacgactac gcgtccatgg
     2521 ctcgaggagc tcggtaccgc cccactaccc gatttatcaa gatttggcgg agagtcccat
     2581 tgacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact
     2641 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2701 gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2761 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag caacgatgtt
     2821 acgcagcagg gcagtcgccc taaaacaaag ttaggtggct caagtatggg catcattcgc
     2881 acatgtaggc tcggccctga ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt
     2941 cgtgagttcg gagacgtagc cacctactcc caacatcagc cggactccga ttacctcggg
     3001 aacttgctcc gtagtaagac attcatcgcg cttgctgcct tcgaccaaga agcggttgtt
     3061 ggcgctctcg cggcttacgt tctgcccaag tttgagcagc cgcgtagtga gatctatatc
     3121 tatgatctcg cagtctccgg cgagcaccgg aggcagggca ttgccaccgc gctcatcaat
     3181 ctcctcaagc atgaggccaa cgcgcttggt gcttatgtga tctacgtgca agcagattac
     3241 ggtgacgatc ccgcagtggc tctctataca aagttgggca tacgggaaga agtgatgcac
     3301 tttgatatcg acccaagtac cgccacctaa gtcgactggc aaacagctat tatgggtatt
     3361 atgggtgtgt atccagaacg acggatcttg acgcaatttg gcgtggctct cttaaggtag
     3421 ccctccgacc aattgaggtg cgctctgacc gatcagttta gacttgtgta taagagtcag
     3481 agcgtcctga acggaacctt tcccgttttc caggatctga tcttccatgt gacctcctaa
     3541 catggtaacg ttcatgataa cttctgctct tcatcgtgcg gccgactggg ctaaatctgt
     3601 gttctcttcg gcggcgctgg gtgatcctcg ccgtactgcc cgcttggtta acgtcgccgc
     3661 ccaattggca aaatattctg gtaaatcaat aaccatctca tcagagggta gtgaagccat
     3721 gcaggaaggc gcttaccgat ttatccgcaa tcccaacgtt tctgccgagg cgatcagaaa
     3781 ggctggcgcc atgcaaacag tcaagttggc tcaggagttt cccgaactgc tggccattga
     3841 ggacaccacc tctttgagtt atcgccacca ggtcgccgaa gagcttggca agctgggctc
     3901 tattcaggat aaatcccgcg gatggtgggt tcactccgtt ctcttgctcg aggccaccac
     3961 attccgcacc gtaggattac tgcatcagga gtggtggatg cgcccggatg accctgccga
     4021 tgcggatgaa aaggagagtg gcaaatggct ggcagcggcc gcaactagcc ggttacgcat
     4081 gggcagcatg atgagcaacg tgattgcggt ctgtgaccgc gaagccgata ttcatgctta
     4141 tctgcaggac aaactggcgc ataacgagcg cttcgtggtg cgctccaagc acccacgcaa
     4201 ggacgtagag tctgggttgt atctgtacga ccatctgaag aaccaaccgg agttgggtgg
     4261 ctatcagatc agcattccgc aaaagggcgt ggtggataaa cgcggtaaac gtaaaaatcg
     4321 accagcccgc aaggcgagct tgagcctgcg cagtgggcgc atcacgctaa aacaggggaa
     4381 tatcacgctc aacgcggtgc tggccgagga gattaacccg cccaagggtg agaccccgtt
     4441 gaaatggttg ttgctgacca gcgaaccggt cgagtcgcta gcccaagcct tgcgcgtcat
     4501 cgacatttat acccatcgct ggcggatcga ggagttccat aaggcatgga aaaccggagc
     4561 aggagccgag aggcaacgca tggaggagcc ggataatctg gagcggatgg tctcgatcct
     4621 ctcgtttgtt gcggtcaggc tgttacagct cagagaaagc ttcacgctgc cgcaagcact
     4681 cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt
     4741 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     4801 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     4861 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tgggaaggtt gggaagccct
     4921 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     4981 ctgacccggg actagttggt gtggaagtcc atatatcggt gatcaatata gtggttgaca
     5041 tgctggctag tcaacattga gccttttgat catgcaaata tattacggta ttttacaatc
     5101 aaatatcaaa cttaactatt gactttataa cttatttagg tggtaacatt cttataaaaa
     5161 agaaaaaaat tactgcaaaa cagtactagc ttttaacttg tatcctaggt tatctatgct
     5221 gtctcaccat agagaatatt acctatttca gaatgtatgt ccatgattcg ccgggtaaat
     5281 acatataata cacaaatctg gcttaataaa gtctataata tatctcataa agaagtgcta
     5341 aattggctag tgctatatat ttttaagaaa atttcttttg actaagtcca tatcgacttt
     5401 gtaaaagttc actttagcat acatatatta cacgagccag aaattgtaac ttttgcctaa
     5461 aatcacaaat tgcaaaattt aattgcttgc aaaaggtcac atgcttataa tcaacttttt
     5521 taaaaattta aaatactttt ttatttttta tttttaaaca taaatgaaat aatttattta
     5581 ttgtttatga ttaccgaaac ataaaacctg ctcaagaaaa agaaactgtt ttgtccttgg
     5641 aaaaaaagca ctacctagga gcggccaaaa tgccgaggct ttcatagctt aaactcttta
     5701 cagaaaatag gcattataga tcagttcgag ttttcttatt cttccttccg gttttatcgt
     5761 cacagtttta cagtaaataa gtatcacctc ttagagttaa ctatgagata agcaagtatc
     5821 atctcatttc atttacctga agtcgagtaa acagaaaatc caattgttga tgaacctcaa
     5881 tgacttagaa ctatctatcg gcagatcata taaagaggat ttaggtactc gagaacgctc
     5941 cgtacatgca gctaaacctc caagcatctc taacttgggt agtgctttct ccaccatttt
     6001 ctgtgcttgc tccttcgtgg caagtccagc ccataatgcc cagaatgtag ttgcggattc
     6061 gtatgacgtt ctgtgcttga tttttgtgtt gtagtcaaag aaaaaccccg actcgtcatc
     6121 ccacatatat ttggtaattg atgaggcaac gctaattatc aacatataga ttgttatcta
     6181 tctgcatgaa cacgaaatct ttacttgacg acttgaggct gatggtgttt atgcaaagaa
     6241 accactgtgt ttaatatgtg tcactgtttg atattactgt cagcgtagaa gataatagta
     6301 aaagcggtta ataagtgtat ttgagataag tgtgataaag tttttacagc gaaaagacga
     6361 taaatacaag aaaatgatta cgaggatacg gagagaggta tgtacatgtg tatttatata
     6421 ctaagctgcc ggcggttgtt tgcaagaccg agaaaaggct agcaagaatc gggtcattgt
     6481 agcgtatgcg cctgtgaaca ttctcttcaa caagtttgat tccattgcgg tgaaatggta
     6541 aaagtcaacc ccctgcgatg tatattttcc tgtacaatca atcaaaaagc caaatgattt
     6601 agcattatct ttacatcttg ttattttaca gattttatgt ttagatcttt tatgcttgct
     6661 tttcaaaagg cctgcaggca agtgcacaaa caatacttaa ataaatacta ctcagtaata
     6721 acctatttct tagcattttt gacgaaattt gctattttgt tagagtcttt tacaccattt
     6781 gtctccacac ctccgcttac atcaacacca ataacgccat ttaatctaag cgcatcacca
     6841 acattttctg gcgtcagtcc accagctaac ataaaatgta agctctgcct cgcgcgtttc
     6901 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     6961 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     7021 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     7081 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     7141 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     7201 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7261 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7321 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7381 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7441 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7501 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7561 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7621 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7681 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7741 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7801 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7861 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7921 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7981 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8041 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8101 ggtctgacag gagctcgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     8161 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     8221 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     8281 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     8341 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atccgcgcct tgagcctggc
     8401 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     8461 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     8521 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     8581 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     8641 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     8701 ggccagccac gatagccgcg ctgcctcgtc ttggagttca ttcagggcac cggacaggtc
     8761 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     8821 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     8881 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     8941 atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     9001 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     9061 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     9121 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggc
     9181 gatcgatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
     9241 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
     9301 gtccgccatc tccagcagcc gcacgcggcg catcgggggg ggggtttcaa ttcaattcat
     9361 catttttttt ttattctttt ttttgatttc ggtttctttg aaattttttt gattcggtaa
     9421 tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca
     9481 tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa
     9541 aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta
     9601 ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact
     9661 tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag
     9721 gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg
     9781 gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca
     9841 gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa
     9901 tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg
     9961 gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag
    10021 aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact gttgacattg
    10081 cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag
    10141 atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg
    10201 cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct gacattatta
    10261 ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca
    10321 gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat
    10381 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt
    10441 tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa aaaattggaa
    10501 agaaaacccc ccccccgcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg
    10561 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg
    10621 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca
    10681 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc
    10741 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
    10801 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
    10861 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
    10921 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
    10981 tcccccttac acggaggcat caagtgacca aacaggaaaa aaccgccctt aacatggccc
    11041 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
    11101 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcaggtggg
    11161 ccattctcat gaagaatatc ttgaatttat tgtcatatta
//
