LOCUS       pYTRW13K_3G5           11191 bp    DNA     circular SYN 27-SEP-2021
DEFINITION  
ACCESSION   pYTRW13K_3G5
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     promoter        104..155
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /label=P mob
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P mob"
                     /SECDescr="mob promoter including oriT (Szpirer et al 2001, PMID: 11222611)."
     CDS             223..1242
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID:
                     19928068328)."
                     /transl_table=11
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mob"
                     /SECDescr="Mob protein necessary for mobilization"
     misc_feature    1243..1261
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-L
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-L"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     misc_feature    1262..1301
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /label=Core_up
                     /SECDrawAs="Label"
                     /SECName="Core_up"
                     /SECDescr="upstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    1302..1316
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    1317..1356
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /SECDrawAs="Label"
                     /SECName="P_up"
                     /SECDescr="upstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1357..1393
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /label=PI-SceI
                     /SECDrawAs="Label"
                     /SECName="PI-SceI"
                     /SECDescr="recognition site of homing endonuclease PI-SceI, enabling e.g. promoter insertion."
     misc_feature    1394..1401
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /label=AsiSI
                     /SECDrawAs="Label"
                     /SECName="AsiSI"
                     /SECDescr="recognition site of endonuclease AsiSI, enabling e.g. promoter insertion."
     misc_feature    1402..1441
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /label=P_dn
                     /SECDrawAs="Label"
                     /SECName="P_dn"
                     /SECDescr="downstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1402..1521
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_up
                     /SECDrawAs="Label"
                     /SECName="CIS_up"
                     /SECDescr="upstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1522..1539
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /label=I-SceI
                     /SECDrawAs="Label"
                     /SECName="I-SceI"
                     /SECDescr="recognition site of homing endonuclease I-SceI, enabling target gene insertion."
     misc_feature    1540..1659
                     /note="downstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_dn
                     /SECDrawAs="Label"
                     /SECName="CIS_dn"
                     /SECDescr="downstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1620..1659
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /gene="R_up"
                     /SECDrawAs="Label"
                     /SECName="R_up"
                     /SECDescr="upstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    1660..1667
                     /label=MauBI
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion/exchange."
     CDS             1689..2399
                     /gene="mCherry"
                     /product="red fluorescent protein mCherry"
                     /transl_table=11
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
                     /note="red fluorescent protein from Discosoma sp. (Shaner
                     et al. 2004, PMID:15558047)."
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mCherry"
                     /SECDescr="red fluorescent protein mCherry"
     misc_feature    2400..2407
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /label=MauBI
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion/exchange."
     misc_feature    2408..2433
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion/exchange."
                     /label=I-CeuI
                     /SECDrawAs="Label"
                     /SECName="I-CeuI"
                     /SECDescr="recognition site of homing endonuclease I-CeuI, enabling e.g. reporter insertion/exchange."
     misc_feature    2434..2473
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /label=R_dn
                     /SECDrawAs="Label"
                     /SECName="R_dn"
                     /SECDescr="downstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    2473..2530
                     /label=MCS Integration site
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /SECDrawAs="Info only"
     misc_feature    2474..2529
                     /note="region with Multiple Cutters
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon
                     Tn5 insertion site elucidation."
                     /label=MC
                     /SECDrawAs="Label"
                     /SECName="MC"
                     /SECDescr="region with Multiple Cutters EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon Tn5 insertion site elucidation."
     misc_feature    2530..2569
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_up
                     /SECDrawAs="Label"
                     /SECName="M_up"
                     /SECDescr="upstream sequence of marker slot (SalI/PI-PspI)."
     mobile_element  complement(2570..3321)
                     /mobile_element_type="transposon:mini-Tn7"
                     /SECDrawAs="Info only"
                     /SECName="'mobile_element'"
     source          complement(2570..3321)
                     /organism="Cloning vector pUC18-mini-Tn7-Gm"
                     /mol_type="other DNA"
                     /db_xref="taxon:295022"
                     /SECDrawAs="Info only"
                     /SECName="'source'"
     promoter        2571..2600
                     /note="aacC1 promoter region (Wohlleben et al 1989, PMID:
                     2549372)."
                     /label=P aacC1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aacC1"
                     /SECDescr="aacC1 promoter region (Wohlleben et al 1989, PMID: 2549372)."
     CDS             2788..3321
                     /gene="aacC1"
                     /transl_table=11
                     /product="gentamicin acetyl transferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL
                     DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQP
                     RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL
                     GIREEVMHFDIDPSTAT"
                     /note="aacC1 from pUCGM (Schweizer 1993, PMID: 8267974)."
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aacC1"
                     /SECDescr="gentamicin acetyl transferase"
     gene            2788..3321
                     /gene="aacC1"
                     /SECDrawAs="Info only"
                     /SECName="aacC1"
     misc_feature    3322..3327
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /label=SalI
                     /SECDrawAs="Label"
                     /SECName="SalI"
                     /SECDescr="recognition site of endonuclease SalI, enabling e.g. marker insertion."
     misc_feature    3328..3357
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /label=PI-PspI
                     /SECDrawAs="Label"
                     /SECName="PI-PspI"
                     /SECDescr="recognition site of homing endonuclease PI-PspI, enabling e.g. marker insertion."
     misc_feature    3358..3397
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_dn
                     /SECDrawAs="Label"
                     /SECName="M_dn"
                     /SECDescr="downstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    3398..3412
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    3413..3452
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /label=Core_dn
                     /SECDrawAs="Label"
                     /SECName="Core_dn"
                     /SECDescr="downstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    complement(3453..3471)
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-R
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-R"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     promoter        3482..3512
                     /note="transposon Tn5 transposase promoter (Yin et al
                     1988, PMID: 2451025)."
                     /label=P tnp
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tnp"
                     /SECDescr="transposon Tn5 transposase promoter (Yin et al 1988, PMID: 2451025)."
     CDS             3545..4975
                     /gene="tnp"
                     /product="transposon Tn5 transposase"
                     /transl_table=11
                     /note="transposon Tn5 transposase (Goryshin & Reznikoff
                     1998, PMID: 9516433)."
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGK
                     SITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLS
                     YRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEK
                     ESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDV
                     ESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGN
                     ITLNAVLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKT
                     GAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQS
                     AETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW
                     EGWEALQSKLDGFLAAKDLMAQGIKI"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tnp"
                     /SECDescr="transposon Tn5 transposase"
     misc_feature    5487..5597
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /label=CEN4
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="CEN4"
                     /SECDescr="CEN4 of Saccharomyces cerevisiae Chr IV (Saccharomyces Genome Database)."
     misc_feature    6129..6873
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /label=ARS1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ARS1 "
                     /SECDescr="ARS1 of yCP50 (Genbank: X70276.1)."
     rep_origin      7343..7931
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387);
                     annotated as New England Biolabs (2007)."
                     /label=pMB1 ori
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pMB1 ori"
                     /SECDescr="origin of replication from plasmid pBR322 (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); annotated as New England Biolabs (2007)."
     CDS             complement(8110..8904)
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /transl_table=11
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aphII"
                     /SECDescr="aminoglycoside 3'-phosphotransferase"
     promoter        8994..9042
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank
                     U00004.1)."
                     /label=P aphII
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aphII"
                     /SECDescr="promoter of aphII gene in Transposon Tn5 (Genbank U00004.1)."
     promoter        9342..9557
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /label=P URA3
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P URA3"
                     /SECDescr="URA3 promoter region (Roy et al 1990, PMID: 2204810)."
     CDS             9558..10361
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="URA3"
                     /SECDescr="orotidine 5'-phosphate decarboxylase"
ORIGIN      
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgaac tttagaagga
     1681 gatataccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     1741 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     1801 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     1861 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     1921 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     1981 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctcct
     2041 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2101 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2161 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2221 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     2281 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     2341 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaac
     2401 gcgcgcgtaa ctataacggt cctaaggtag cgagcttact tgtgctgttg tgtaaggaca
     2461 cgcttaaccc agagaattcc agcgacctat tctacgacta cgcgtccatg gctcgaggag
     2521 ctcggtaccg ccccactacc cgatttatca agatttggcg gagagtccca ttgacataag
     2581 cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2641 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2701 gtttttttgt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2761 gatgtttgat gttatggagc agcaacgatg ttacgcagca gcaacgatgt tacgcagcag
     2821 ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg cacatgtagg
     2881 ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg tcgtgagttc
     2941 ggagacgtag ccacctactc ccaacatcag ccggactccg attacctcgg gaacttgctc
     3001 cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc
     3061 gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg agatctatat ctatgatctc
     3121 gcagtctccg gcgagcaccg gaggcagggc attgccaccg cgctcatcaa tctcctcaag
     3181 catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta cggtgacgat
     3241 cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca ctttgatatc
     3301 gacccaagta ccgccaccta agtcgactgg caaacagcta ttatgggtat tatgggtgtg
     3361 tatccagaac gacggatctt gacgcaattt ggcgtggctc tcttaaggta gccctccgac
     3421 caattgaggt gcgctctgac cgatcagttt agacttgtgt ataagagtca gagcgtcctg
     3481 aacggaacct ttcccgtttt ccaggatctg atcttccatg tgacctccta acatggtaac
     3541 gttcatgata acttctgctc ttcatcgtgc ggccgactgg gctaaatctg tgttctcttc
     3601 ggcggcgctg ggtgatcctc gccgtactgc ccgcttggtt aacgtcgccg cccaattggc
     3661 aaaatattct ggtaaatcaa taaccatctc atcagagggt agtgaagcca tgcaggaagg
     3721 cgcttaccga tttatccgca atcccaacgt ttctgccgag gcgatcagaa aggctggcgc
     3781 catgcaaaca gtcaagttgg ctcaggagtt tcccgaactg ctggccattg aggacaccac
     3841 ctctttgagt tatcgccacc aggtcgccga agagcttggc aagctgggct ctattcagga
     3901 taaatcccgc ggatggtggg ttcactccgt tctcttgctc gaggccacca cattccgcac
     3961 cgtaggatta ctgcatcagg agtggtggat gcgcccggat gaccctgccg atgcggatga
     4021 aaaggagagt ggcaaatggc tggcagcggc cgcaactagc cggttacgca tgggcagcat
     4081 gatgagcaac gtgattgcgg tctgtgaccg cgaagccgat attcatgctt atctgcagga
     4141 caaactggcg cataacgagc gcttcgtggt gcgctccaag cacccacgca aggacgtaga
     4201 gtctgggttg tatctgtacg accatctgaa gaaccaaccg gagttgggtg gctatcagat
     4261 cagcattccg caaaagggcg tggtggataa acgcggtaaa cgtaaaaatc gaccagcccg
     4321 caaggcgagc ttgagcctgc gcagtgggcg catcacgcta aaacagggga atatcacgct
     4381 caacgcggtg ctggccgagg agattaaccc gcccaagggt gagaccccgt tgaaatggtt
     4441 gttgctgacc agcgaaccgg tcgagtcgct agcccaagcc ttgcgcgtca tcgacattta
     4501 tacccatcgc tggcggatcg aggagttcca taaggcatgg aaaaccggag caggagccga
     4561 gaggcaacgc atggaggagc cggataatct ggagcggatg gtctcgatcc tctcgtttgt
     4621 tgcggtcagg ctgttacagc tcagagaaag cttcacgctg ccgcaagcac tcagggcgca
     4681 agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc
     4741 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc
     4801 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
     4861 gcgaaccgga attgccagct ggggcgccct ctgggaaggt tgggaagccc tgcaaagtaa
     4921 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgacccgg
     4981 gactagttgg tgtggaagtc catatatcgg tgatcaatat agtggttgac atgctggcta
     5041 gtcaacattg agccttttga tcatgcaaat atattacggt attttacaat caaatatcaa
     5101 acttaactat tgactttata acttatttag gtggtaacat tcttataaaa aagaaaaaaa
     5161 ttactgcaaa acagtactag cttttaactt gtatcctagg ttatctatgc tgtctcacca
     5221 tagagaatat tacctatttc agaatgtatg tccatgattc gccgggtaaa tacatataat
     5281 acacaaatct ggcttaataa agtctataat atatctcata aagaagtgct aaattggcta
     5341 gtgctatata tttttaagaa aatttctttt gactaagtcc atatcgactt tgtaaaagtt
     5401 cactttagca tacatatatt acacgagcca gaaattgtaa cttttgccta aaatcacaaa
     5461 ttgcaaaatt taattgcttg caaaaggtca catgcttata atcaactttt ttaaaaattt
     5521 aaaatacttt tttatttttt atttttaaac ataaatgaaa taatttattt attgtttatg
     5581 attaccgaaa cataaaacct gctcaagaaa aagaaactgt tttgtccttg gaaaaaaagc
     5641 actacctagg agcggccaaa atgccgaggc tttcatagct taaactcttt acagaaaata
     5701 ggcattatag atcagttcga gttttcttat tcttccttcc ggttttatcg tcacagtttt
     5761 acagtaaata agtatcacct cttagagtta actatgagat aagcaagtat catctcattt
     5821 catttacctg aagtcgagta aacagaaaat ccaattgttg atgaacctca atgacttaga
     5881 actatctatc ggcagatcat ataaagagga tttaggtact cgagaacgct ccgtacatgc
     5941 agctaaacct ccaagcatct ctaacttggg tagtgctttc tccaccattt tctgtgcttg
     6001 ctccttcgtg gcaagtccag cccataatgc ccagaatgta gttgcggatt cgtatgacgt
     6061 tctgtgcttg atttttgtgt tgtagtcaaa gaaaaacccc gactcgtcat cccacatata
     6121 tttggtaatt gatgaggcaa cgctaattat caacatatag attgttatct atctgcatga
     6181 acacgaaatc tttacttgac gacttgaggc tgatggtgtt tatgcaaaga aaccactgtg
     6241 tttaatatgt gtcactgttt gatattactg tcagcgtaga agataatagt aaaagcggtt
     6301 aataagtgta tttgagataa gtgtgataaa gtttttacag cgaaaagacg ataaatacaa
     6361 gaaaatgatt acgaggatac ggagagaggt atgtacatgt gtatttatat actaagctgc
     6421 cggcggttgt ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg tagcgtatgc
     6481 gcctgtgaac attctcttca acaagtttga ttccattgcg gtgaaatggt aaaagtcaac
     6541 cccctgcgat gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt tagcattatc
     6601 tttacatctt gttattttac agattttatg tttagatctt ttatgcttgc ttttcaaaag
     6661 gcctgcaggc aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc
     6721 ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca
     6781 cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct
     6841 ggcgtcagtc caccagctaa cataaaatgt aagctctgcc tcgcgcgttt cggtgatgac
     6901 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     6961 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     7021 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     7081 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     7141 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7741 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8101 ggagctcgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     8161 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     8221 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     8281 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     8341 atgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg cgaacagttc
     8401 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     8461 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     8521 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     8581 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     8641 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     8701 cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt cggtcttgac
     8761 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     8821 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     8881 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatct
     8941 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     9001 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     9061 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     9121 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg cgatcgatgg
     9181 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
     9241 gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat
     9301 ctccagcagc cgcacgcggc gcatcggggg gggggtttca attcaattca tcattttttt
     9361 tttattcttt tttttgattt cggtttcttt gaaatttttt tgattcggta atctccgaac
     9421 agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg
     9481 ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca
     9541 ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta
     9601 gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt
     9661 cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa
     9721 tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta
     9781 agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg
     9841 ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat
     9901 gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc
     9961 aggcggcaga agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat
    10021 gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg
    10081 acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt
    10141 acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc
    10201 aacagtatag aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa
    10261 gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag
    10321 gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa
    10381 atgcatgtat actaaactca caaattagag cttcaattta attatatcag ttattacccg
    10441 ggaatctcgg tcgtaatgat ttttataatg acgaaaaaaa aaaaattgga aagaaaaccc
    10501 cccccccgca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg
    10561 acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag
    10621 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc
    10681 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg
    10741 ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta
    10801 acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc
    10861 agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc
    10921 gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa ttccccctta
    10981 cacggaggca tcaagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
    11041 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
    11101 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcaggtgg gccattctca
    11161 tgaagaatat cttgaattta ttgtcatatt a
//
