LOCUS       pYTRW18K_3T5           11712 bp    DNA     circular SYN 27-SEP-2021
DEFINITION  
ACCESSION   pYTRW18K_3T5
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     promoter        104..155
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /label=P mob
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P mob"
                     /SECDescr="mob promoter including oriT (Szpirer et al 2001, PMID: 11222611)."
     CDS             223..1242
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID:
                     19928068328)."
                     /transl_table=11
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mob"
                     /SECDescr="Mob protein necessary for mobilization"
     misc_feature    1243..1261
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-L
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-L"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     misc_feature    1262..1301
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /label=Core_up
                     /SECDrawAs="Label"
                     /SECName="Core_up"
                     /SECDescr="upstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    1302..1316
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    1317..1356
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /SECDrawAs="Label"
                     /SECName="P_up"
                     /SECDescr="upstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1357..1393
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /label=PI-SceI
                     /SECDrawAs="Label"
                     /SECName="PI-SceI"
                     /SECDescr="recognition site of homing endonuclease PI-SceI, enabling e.g. promoter insertion."
     misc_feature    1394..1401
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /label=AsiSI
                     /SECDrawAs="Label"
                     /SECName="AsiSI"
                     /SECDescr="recognition site of endonuclease AsiSI, enabling e.g. promoter insertion."
     misc_feature    1402..1441
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /label=P_dn
                     /SECDrawAs="Label"
                     /SECName="P_dn"
                     /SECDescr="downstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1402..1521
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_up
                     /SECDrawAs="Label"
                     /SECName="CIS_up"
                     /SECDescr="upstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1522..1539
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /label=I-SceI
                     /SECDrawAs="Label"
                     /SECName="I-SceI"
                     /SECDescr="recognition site of homing endonuclease I-SceI, enabling target gene insertion."
     misc_feature    1540..1659
                     /note="downstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_dn
                     /SECDrawAs="Label"
                     /SECName="CIS_dn"
                     /SECDescr="downstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    1620..1659
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /gene="R_up"
                     /SECDrawAs="Label"
                     /SECName="R_up"
                     /SECDescr="upstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    1660..1667
                     /label=MauBI
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion/exchange."
     CDS             1689..2399
                     /gene="mCherry"
                     /transl_table=11
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
                     /product="red fluorescent protein mCherry"
                     /note="red fluorescent protein from Discosoma sp. (Shaner
                     et al. 2004, PMID:15558047)."
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mCherry"
                     /SECDescr="red fluorescent protein mCherry"
     misc_feature    2400..2407
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /label=MauBI
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion/exchange."
     misc_feature    2408..2433
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion/exchange."
                     /label=I-CeuI
                     /SECDrawAs="Label"
                     /SECName="I-CeuI"
                     /SECDescr="recognition site of homing endonuclease I-CeuI, enabling e.g. reporter insertion/exchange."
     misc_feature    2434..2473
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /label=R_dn
                     /SECDrawAs="Label"
                     /SECName="R_dn"
                     /SECDescr="downstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    2473..2530
                     /label=MCS Integration site
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /SECDrawAs="Info only"
     misc_feature    2474..2529
                     /note="region with Multiple Cutters
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon
                     Tn5 insertion site elucidation."
                     /label=MC
                     /SECDrawAs="Label"
                     /SECName="MC"
                     /SECDescr="region with Multiple Cutters EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon Tn5 insertion site elucidation."
     misc_feature    2530..2569
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_up
                     /SECDrawAs="Label"
                     /SECName="M_up"
                     /SECDescr="upstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    2570..2629
                     /label=P tet
                     /note="tet promoter region (Harley et al 1988, PMID:
                     3045754)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tet"
                     /SECDescr="tet promoter region (Harley et al 1988, PMID: 3045754)."
     CDS             2652..3842
                     /gene="tet"
                     /product="tetracycline effux pump"
                     /transl_table=11
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
                     /note="tet gene, TcR from pBR322 (Bolivar 1977, PMID:
                     344137; Sutcliffe 1979, PMID: 383387)."
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tet"
                     /SECDescr="tetracycline effux pump"
     misc_feature    3843..3848
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /label=SalI
                     /SECDrawAs="Label"
                     /SECName="SalI"
                     /SECDescr="recognition site of endonuclease SalI, enabling e.g. marker insertion."
     misc_feature    3849..3878
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /label=PI-PspI
                     /SECDrawAs="Label"
                     /SECName="PI-PspI"
                     /SECDescr="recognition site of homing endonuclease PI-PspI, enabling e.g. marker insertion."
     misc_feature    3879..3918
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_dn
                     /SECDrawAs="Label"
                     /SECName="M_dn"
                     /SECDescr="downstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    3919..3933
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    3934..3973
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /label=Core_dn
                     /SECDrawAs="Label"
                     /SECName="Core_dn"
                     /SECDescr="downstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    complement(3974..3992)
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /label=OE-R
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-R"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     promoter        4003..4033
                     /note="transposon Tn5 transposase promoter (Yin et al
                     1988, PMID: 2451025)."
                     /label=P tnp
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tnp"
                     /SECDescr="transposon Tn5 transposase promoter (Yin et al 1988, PMID: 2451025)."
     CDS             4066..5496
                     /gene="tnp"
                     /product="transposon Tn5 transposase"
                     /transl_table=11
                     /note="transposon Tn5 transposase (Goryshin & Reznikoff
                     1998, PMID: 9516433)."
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGK
                     SITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLS
                     YRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEK
                     ESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDV
                     ESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGN
                     ITLNAVLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKT
                     GAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQS
                     AETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW
                     EGWEALQSKLDGFLAAKDLMAQGIKI"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tnp"
                     /SECDescr="transposon Tn5 transposase"
     misc_feature    6008..6118
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /label=CEN4
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="CEN4"
                     /SECDescr="CEN4 of Saccharomyces cerevisiae Chr IV (Saccharomyces Genome Database)."
     misc_feature    6650..7394
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /label=ARS1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ARS1 "
                     /SECDescr="ARS1 of yCP50 (Genbank: X70276.1)."
     rep_origin      7864..8452
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387);
                     annotated as New England Biolabs (2007)."
                     /label=pMB1 ori
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pMB1 ori"
                     /SECDescr="origin of replication from plasmid pBR322 (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); annotated as New England Biolabs (2007)."
     CDS             complement(8631..9425)
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /transl_table=11
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aphII"
                     /SECDescr="aminoglycoside 3'-phosphotransferase"
     promoter        9515..9563
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank
                     U00004.1)."
                     /label=P aphII
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aphII"
                     /SECDescr="promoter of aphII gene in Transposon Tn5 (Genbank U00004.1)."
     promoter        9863..10078
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /label=P URA3
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P URA3"
                     /SECDescr="URA3 promoter region (Roy et al 1990, PMID: 2204810)."
     CDS             10079..10882
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="URA3"
                     /SECDescr="orotidine 5'-phosphate decarboxylase"
ORIGIN      
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgaac tttagaagga
     1681 gatataccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     1741 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     1801 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     1861 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     1921 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     1981 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctcct
     2041 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2101 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2161 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2221 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     2281 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     2341 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaac
     2401 gcgcgcgtaa ctataacggt cctaaggtag cgagcttact tgtgctgttg tgtaaggaca
     2461 cgcttaaccc agagaattcc agcgacctat tctacgacta cgcgtccatg gctcgaggag
     2521 ctcggtaccg ccccactacc cgatttatca agatttggcg gagagtccct catgtttgac
     2581 agcttatcat cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc
     2641 aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg
     2701 atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc
     2761 attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat
     2821 ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc
     2881 tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt
     2941 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     3001 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     3061 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     3121 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     3181 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct
     3241 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     3301 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     3361 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     3421 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     3481 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     3541 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     3601 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     3661 aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg
     3721 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
     3781 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
     3841 gagtcgactg gcaaacagct attatgggta ttatgggtgt gtatccagaa cgacggatct
     3901 tgacgcaatt tggcgtggct ctcttaaggt agccctccga ccaattgagg tgcgctctga
     3961 ccgatcagtt tagacttgtg tataagagtc agagcgtcct gaacggaacc tttcccgttt
     4021 tccaggatct gatcttccat gtgacctcct aacatggtaa cgttcatgat aacttctgct
     4081 cttcatcgtg cggccgactg ggctaaatct gtgttctctt cggcggcgct gggtgatcct
     4141 cgccgtactg cccgcttggt taacgtcgcc gcccaattgg caaaatattc tggtaaatca
     4201 ataaccatct catcagaggg tagtgaagcc atgcaggaag gcgcttaccg atttatccgc
     4261 aatcccaacg tttctgccga ggcgatcaga aaggctggcg ccatgcaaac agtcaagttg
     4321 gctcaggagt ttcccgaact gctggccatt gaggacacca cctctttgag ttatcgccac
     4381 caggtcgccg aagagcttgg caagctgggc tctattcagg ataaatcccg cggatggtgg
     4441 gttcactccg ttctcttgct cgaggccacc acattccgca ccgtaggatt actgcatcag
     4501 gagtggtgga tgcgcccgga tgaccctgcc gatgcggatg aaaaggagag tggcaaatgg
     4561 ctggcagcgg ccgcaactag ccggttacgc atgggcagca tgatgagcaa cgtgattgcg
     4621 gtctgtgacc gcgaagccga tattcatgct tatctgcagg acaaactggc gcataacgag
     4681 cgcttcgtgg tgcgctccaa gcacccacgc aaggacgtag agtctgggtt gtatctgtac
     4741 gaccatctga agaaccaacc ggagttgggt ggctatcaga tcagcattcc gcaaaagggc
     4801 gtggtggata aacgcggtaa acgtaaaaat cgaccagccc gcaaggcgag cttgagcctg
     4861 cgcagtgggc gcatcacgct aaaacagggg aatatcacgc tcaacgcggt gctggccgag
     4921 gagattaacc cgcccaaggg tgagaccccg ttgaaatggt tgttgctgac cagcgaaccg
     4981 gtcgagtcgc tagcccaagc cttgcgcgtc atcgacattt atacccatcg ctggcggatc
     5041 gaggagttcc ataaggcatg gaaaaccgga gcaggagccg agaggcaacg catggaggag
     5101 ccggataatc tggagcggat ggtctcgatc ctctcgtttg ttgcggtcag gctgttacag
     5161 ctcagagaaa gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg
     5221 gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
     5281 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
     5341 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
     5401 tggggcgccc tctgggaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
     5461 gccaaggatc tgatggcgca ggggatcaag atctgacccg ggactagttg gtgtggaagt
     5521 ccatatatcg gtgatcaata tagtggttga catgctggct agtcaacatt gagccttttg
     5581 atcatgcaaa tatattacgg tattttacaa tcaaatatca aacttaacta ttgactttat
     5641 aacttattta ggtggtaaca ttcttataaa aaagaaaaaa attactgcaa aacagtacta
     5701 gcttttaact tgtatcctag gttatctatg ctgtctcacc atagagaata ttacctattt
     5761 cagaatgtat gtccatgatt cgccgggtaa atacatataa tacacaaatc tggcttaata
     5821 aagtctataa tatatctcat aaagaagtgc taaattggct agtgctatat atttttaaga
     5881 aaatttcttt tgactaagtc catatcgact ttgtaaaagt tcactttagc atacatatat
     5941 tacacgagcc agaaattgta acttttgcct aaaatcacaa attgcaaaat ttaattgctt
     6001 gcaaaaggtc acatgcttat aatcaacttt tttaaaaatt taaaatactt ttttattttt
     6061 tatttttaaa cataaatgaa ataatttatt tattgtttat gattaccgaa acataaaacc
     6121 tgctcaagaa aaagaaactg ttttgtcctt ggaaaaaaag cactacctag gagcggccaa
     6181 aatgccgagg ctttcatagc ttaaactctt tacagaaaat aggcattata gatcagttcg
     6241 agttttctta ttcttccttc cggttttatc gtcacagttt tacagtaaat aagtatcacc
     6301 tcttagagtt aactatgaga taagcaagta tcatctcatt tcatttacct gaagtcgagt
     6361 aaacagaaaa tccaattgtt gatgaacctc aatgacttag aactatctat cggcagatca
     6421 tataaagagg atttaggtac tcgagaacgc tccgtacatg cagctaaacc tccaagcatc
     6481 tctaacttgg gtagtgcttt ctccaccatt ttctgtgctt gctccttcgt ggcaagtcca
     6541 gcccataatg cccagaatgt agttgcggat tcgtatgacg ttctgtgctt gatttttgtg
     6601 ttgtagtcaa agaaaaaccc cgactcgtca tcccacatat atttggtaat tgatgaggca
     6661 acgctaatta tcaacatata gattgttatc tatctgcatg aacacgaaat ctttacttga
     6721 cgacttgagg ctgatggtgt ttatgcaaag aaaccactgt gtttaatatg tgtcactgtt
     6781 tgatattact gtcagcgtag aagataatag taaaagcggt taataagtgt atttgagata
     6841 agtgtgataa agtttttaca gcgaaaagac gataaataca agaaaatgat tacgaggata
     6901 cggagagagg tatgtacatg tgtatttata tactaagctg ccggcggttg tttgcaagac
     6961 cgagaaaagg ctagcaagaa tcgggtcatt gtagcgtatg cgcctgtgaa cattctcttc
     7021 aacaagtttg attccattgc ggtgaaatgg taaaagtcaa ccccctgcga tgtatatttt
     7081 cctgtacaat caatcaaaaa gccaaatgat ttagcattat ctttacatct tgttatttta
     7141 cagattttat gtttagatct tttatgcttg cttttcaaaa ggcctgcagg caagtgcaca
     7201 aacaatactt aaataaatac tactcagtaa taacctattt cttagcattt ttgacgaaat
     7261 ttgctatttt gttagagtct tttacaccat ttgtctccac acctccgctt acatcaacac
     7321 caataacgcc atttaatcta agcgcatcac caacattttc tggcgtcagt ccaccagcta
     7381 acataaaatg taagctctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     7441 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     7501 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     7561 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     7621 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     7681 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7741 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7801 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7861 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7921 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7981 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8041 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8101 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8161 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8221 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8281 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8341 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8401 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8461 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8521 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8581 taaatcaatc taaagtatat atgagtaaac ttggtctgac aggagctcgc tcagaagaac
     8641 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     8701 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     8761 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     8821 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     8881 tcgccgtcgg gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     8941 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     9001 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     9061 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     9121 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     9181 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     9241 tcttggagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     9301 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     9361 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     9421 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
     9481 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
     9541 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
     9601 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
     9661 cagatagccc agtagctgac attcatccgg gcgatcgatg gaagccggcg gcacctcgct
     9721 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
     9781 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
     9841 cgcatcgggg ggggggtttc aattcaattc atcatttttt ttttattctt ttttttgatt
     9901 tcggtttctt tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa
     9961 ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg
    10021 cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat
    10081 gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct
    10141 atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac
    10201 caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca
    10261 tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc
    10321 cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt
    10381 caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc
    10441 acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac
    10501 aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac
    10561 tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg
    10621 ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac
    10681 acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga
    10741 tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg
    10801 aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag
    10861 aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc
    10921 acaaattaga gcttcaattt aattatatca gttattaccc gggaatctcg gtcgtaatga
    10981 tttttataat gacgaaaaaa aaaaaattgg aaagaaaacc ccccccccgc agcgttgggt
    11041 cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg
    11101 gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct
    11161 gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt
    11221 aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag
    11281 gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac
    11341 cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac
    11401 gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt
    11461 tcatcggtat cattaccccc atgaacagaa attccccctt acacggaggc atcaagtgac
    11521 caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct
    11581 tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca
    11641 cgaccacgct gatgagcttt accgcaggtg ggccattctc atgaagaata tcttgaattt
    11701 attgtcatat ta
//
