LOCUS       pYTRW20K_0Ti1          10418 bp    DNA     circular SYN 27-SEP-2021
DEFINITION  
ACCESSION   pYTRW20K_0Ti1
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     promoter        104..155
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /label=P mob
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P mob"
                     /SECDescr="mob promoter including oriT (Szpirer et al 2001, PMID: 11222611)."
     CDS             223..1242
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID:
                     19928068328)."
                     /transl_table=11
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mob"
                     /SECDescr="Mob protein necessary for mobilization"
     misc_feature    1243..1742
                     /label=LP-L
                     /note="left side of integron landing pad sequence"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LP-L"
                     /SECDescr="left side of integron landing pad sequence"
     misc_feature    1743..1782
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /label=Core_up
                     /SECDrawAs="Label"
                     /SECName="Core_up"
                     /SECDescr="upstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    1783..1797
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    1798..1837
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /SECDrawAs="Label"
                     /SECName="P_up"
                     /SECDescr="upstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1838..1874
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /label=PI-SceI
                     /SECDrawAs="Label"
                     /SECName="PI-SceI"
                     /SECDescr="recognition site of homing endonuclease PI-SceI, enabling e.g. promoter insertion."
     misc_feature    1875..1882
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /label=AsiSI
                     /SECDrawAs="Label"
                     /SECName="AsiSI"
                     /SECDescr="recognition site of endonuclease AsiSI, enabling e.g. promoter insertion."
     misc_feature    1883..1922
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /label=P_dn
                     /SECDrawAs="Label"
                     /SECName="P_dn"
                     /SECDescr="downstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1883..2002
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_up
                     /SECDrawAs="Label"
                     /SECName="CIS_up"
                     /SECDescr="upstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    2003..2020
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /label=I-SceI
                     /SECDrawAs="Label"
                     /SECName="I-SceI"
                     /SECDescr="recognition site of homing endonuclease I-SceI, enabling target gene insertion."
     misc_feature    2021..2140
                     /note="downstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_dn
                     /SECDrawAs="Label"
                     /SECName="CIS_dn"
                     /SECDescr="downstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    2101..2140
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /gene="R_up"
                     /SECDrawAs="Label"
                     /SECName="R_up"
                     /SECDescr="upstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    2141..2148
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion."
                     /label=MauBI
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion."
     misc_feature    2149..2174
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion."
                     /label=I-CeuI
                     /SECDrawAs="Label"
                     /SECName="I-CeuI"
                     /SECDescr="recognition site of homing endonuclease I-CeuI, enabling e.g. reporter insertion."
     misc_feature    2175..2214
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /label=R_dn
                     /SECDrawAs="Label"
                     /SECName="R_dn"
                     /SECDescr="downstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    2214..2271
                     /label=MCS Integration site
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /SECDrawAs="Info only"
     misc_feature    2215..2270
                     /note="region with Multiple Cutters
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon
                     Tn5 insertion site elucidation."
                     /label=MC
                     /SECDrawAs="Label"
                     /SECName="MC"
                     /SECDescr="region with Multiple Cutters EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon Tn5 insertion site elucidation."
     misc_feature    2271..2310
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_up
                     /SECDrawAs="Label"
                     /SECName="M_up"
                     /SECDescr="upstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    2311..2370
                     /label=P tet
                     /note="tet promoter region (Harley et al 1988, PMID:
                     3045754)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tet"
                     /SECDescr="tet promoter region (Harley et al 1988, PMID: 3045754)."
     CDS             2393..3583
                     /gene="tet"
                     /product="tetracycline effux pump"
                     /transl_table=11
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
                     /note="tet gene, TcR from pBR322 (Bolivar 1977, PMID:
                     344137; Sutcliffe 1979, PMID: 383387)."
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tet"
                     /SECDescr="tetracycline effux pump"
     misc_feature    3584..3589
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /label=SalI
                     /SECDrawAs="Label"
                     /SECName="SalI"
                     /SECDescr="recognition site of endonuclease SalI, enabling e.g. marker insertion."
     misc_feature    3590..3619
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /label=PI-PspI
                     /SECDrawAs="Label"
                     /SECName="PI-PspI"
                     /SECDescr="recognition site of homing endonuclease PI-PspI, enabling e.g. marker insertion."
     misc_feature    3620..3659
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_dn
                     /SECDrawAs="Label"
                     /SECName="M_dn"
                     /SECDescr="downstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    3660..3674
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    3675..3714
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /label=Core_dn
                     /SECDrawAs="Label"
                     /SECName="Core_dn"
                     /SECDescr="downstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    3715..4214
                     /label=LP-R
                     /note="right side of integron landing pad sequence"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LP-R"
                     /SECDescr="right side of integron landing pad sequence"
     misc_feature    4714..4824
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /label=CEN4
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="CEN4"
                     /SECDescr="CEN4 of Saccharomyces cerevisiae Chr IV (Saccharomyces Genome Database)."
     misc_feature    5356..6100
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /label=ARS1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ARS1 "
                     /SECDescr="ARS1 of yCP50 (Genbank: X70276.1)."
     rep_origin      6570..7158
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387);
                     annotated as New England Biolabs (2007)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pMB1 ori"
                     /SECDescr="origin of replication from plasmid pBR322 (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); annotated as New England Biolabs (2007)."
     CDS             complement(7337..8131)
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /transl_table=11
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aphII"
                     /SECDescr="aminoglycoside 3'-phosphotransferase"
     promoter        8221..8269
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank
                     U00004.1)."
                     /label=P aphII
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aphII"
                     /SECDescr="promoter of aphII gene in Transposon Tn5 (Genbank U00004.1)."
     promoter        8569..8784
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /label=P URA3
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P URA3"
                     /SECDescr="URA3 promoter region (Roy et al 1990, PMID: 2204810)."
     CDS             8785..9588
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="URA3"
                     /SECDescr="orotidine 5'-phosphate decarboxylase"
ORIGIN      
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gagacgccac gcgatcgaac
     1261 gtcgttaact ctggcaggca attaaaggga acgtgtatct gacgcaaaga aaactggaaa
     1321 ttggcgagag aatcttctct ctgtctatcg aagactgacc acgcggaggc gtgcgtcttg
     1381 ttagcgtgcg gggtactctt gctatccatt tgagtcacac gacactcgct gttctcgact
     1441 ttacacttgt aagcgacggt attgaacgac gcttctgccc accatcgtta caaccagact
     1501 gatactagat ctatactgtc agccattcag acgaaaccag ccggagatta ccgagcactc
     1561 tattacgtcg gcgaccacta ctgagctagt ggagccgtgg gtaacgatca taccactaag
     1621 aacctctctc gacgcaagcg attacactgc tgtcacatca taatcgtctt gctattcacg
     1681 agttgaccag acactggata gcttgtcact tgaagtattc tgcacgacag ggtgcgtgta
     1741 cctatctgca gcactcaaca gatctccgtc tacttgcgac acctctctta aggtagctgg
     1801 accggattcg ggtctagcct atatcgtcaa tccgttgatc tatgtcgggt gcggagaaag
     1861 aggtaatgaa atgggcgatc gcccacagct tacacagacc taatcgtaga gttcctagta
     1921 atacttagtg gctcgttacc tcgttcttcc aggccagggg gccgtacgcg ctcggcgaac
     1981 agtataagca gattcgactc actagggata acagggtaat ctctgggtat cgactcaatc
     2041 tcgcgctatt gtgctatatc cgtggacggt tgcggtcaat tctgtcggaa aatcaacgga
     2101 tacgaacaat tactcgcgac ttcctcaata cgtgctgcaa cgcgcgcgta actataacgg
     2161 tcctaaggta gcgagcttac ttgtgctgtt gtgtaaggac acgcttaacc cagagaattc
     2221 cagcgaccta ttctacgact acgcgtccat ggctcgagga gctcggtacc gccccactac
     2281 ccgatttatc aagatttggc ggagagtccc tcatgtttga cagcttatca tcgataagct
     2341 ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc
     2401 taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt
     2461 ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag
     2521 tcactatggc gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct
     2581 cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc
     2641 cactatcgac tacgcgatca tggcgaccac acccgtcctg tggatcctct acgccggacg
     2701 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     2761 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     2821 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     2881 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     2941 gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     3001 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     3061 actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg
     3121 gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca
     3181 agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg
     3241 catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc
     3301 cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat
     3361 gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct
     3421 taccagccta acttcgatca ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc
     3481 gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc
     3541 cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgagtcgact ggcaaacagc
     3601 tattatgggt attatgggtg tgtatccaga acgacggatc ttgacgcaat ttggcgtggc
     3661 tctcttaagg tagccctccg accaattgag gtgcgctctg accgatcagt ttagggtgag
     3721 ccaacccggc cgatccggag agacgggcct caaagccgcg tgacgacggc tgtgggcccg
     3781 taacaaaatc cccgcaataa gctcccgtga gcgtcggttg aacagccctg gtcggcccca
     3841 tcagtagccc gaatacttcg ctttacgggt cctgggccgg ggtgcgatac cttgcagaaa
     3901 tcgaggccgt tcgttaattc ctgttgcatt cgtaccgcct atatttgtct ctttgccggc
     3961 ttatcatcac aagcatagca tagccattta tcggagcgcc tccgtacacg gtatgatcgg
     4021 acgcctcgtg agatcaatac gtataccagg tgtcctgtga gcagcgaaag cctatacgcg
     4081 agatacactg ccaaaaatcc gcgtgattac gagtcgtggc aaatttggtc tggctgtggt
     4141 ctagacattc caggcggtgc gtctgctctc gggtgcctct agtggctggc tagatagact
     4201 agccgctggt aaactggtgt ggaagtccat atatcggtga tcaatatagt ggttgacatg
     4261 ctggctagtc aacattgagc cttttgatca tgcaaatata ttacggtatt ttacaatcaa
     4321 atatcaaact taactattga ctttataact tatttaggtg gtaacattct tataaaaaag
     4381 aaaaaaatta ctgcaaaaca gtactagctt ttaacttgta tcctaggtta tctatgctgt
     4441 ctcaccatag agaatattac ctatttcaga atgtatgtcc atgattcgcc gggtaaatac
     4501 atataataca caaatctggc ttaataaagt ctataatata tctcataaag aagtgctaaa
     4561 ttggctagtg ctatatattt ttaagaaaat ttcttttgac taagtccata tcgactttgt
     4621 aaaagttcac tttagcatac atatattaca cgagccagaa attgtaactt ttgcctaaaa
     4681 tcacaaattg caaaatttaa ttgcttgcaa aaggtcacat gcttataatc aactttttta
     4741 aaaatttaaa atactttttt attttttatt tttaaacata aatgaaataa tttatttatt
     4801 gtttatgatt accgaaacat aaaacctgct caagaaaaag aaactgtttt gtccttggaa
     4861 aaaaagcact acctaggagc ggccaaaatg ccgaggcttt catagcttaa actctttaca
     4921 gaaaataggc attatagatc agttcgagtt ttcttattct tccttccggt tttatcgtca
     4981 cagttttaca gtaaataagt atcacctctt agagttaact atgagataag caagtatcat
     5041 ctcatttcat ttacctgaag tcgagtaaac agaaaatcca attgttgatg aacctcaatg
     5101 acttagaact atctatcggc agatcatata aagaggattt aggtactcga gaacgctccg
     5161 tacatgcagc taaacctcca agcatctcta acttgggtag tgctttctcc accattttct
     5221 gtgcttgctc cttcgtggca agtccagccc ataatgccca gaatgtagtt gcggattcgt
     5281 atgacgttct gtgcttgatt tttgtgttgt agtcaaagaa aaaccccgac tcgtcatccc
     5341 acatatattt ggtaattgat gaggcaacgc taattatcaa catatagatt gttatctatc
     5401 tgcatgaaca cgaaatcttt acttgacgac ttgaggctga tggtgtttat gcaaagaaac
     5461 cactgtgttt aatatgtgtc actgtttgat attactgtca gcgtagaaga taatagtaaa
     5521 agcggttaat aagtgtattt gagataagtg tgataaagtt tttacagcga aaagacgata
     5581 aatacaagaa aatgattacg aggatacgga gagaggtatg tacatgtgta tttatatact
     5641 aagctgccgg cggttgtttg caagaccgag aaaaggctag caagaatcgg gtcattgtag
     5701 cgtatgcgcc tgtgaacatt ctcttcaaca agtttgattc cattgcggtg aaatggtaaa
     5761 agtcaacccc ctgcgatgta tattttcctg tacaatcaat caaaaagcca aatgatttag
     5821 cattatcttt acatcttgtt attttacaga ttttatgttt agatctttta tgcttgcttt
     5881 tcaaaaggcc tgcaggcaag tgcacaaaca atacttaaat aaatactact cagtaataac
     5941 ctatttctta gcatttttga cgaaatttgc tattttgtta gagtctttta caccatttgt
     6001 ctccacacct ccgcttacat caacaccaat aacgccattt aatctaagcg catcaccaac
     6061 attttctggc gtcagtccac cagctaacat aaaatgtaag ctctgcctcg cgcgtttcgg
     6121 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6181 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     6241 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     6301 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     6361 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6541 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6601 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6661 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6721 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6781 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6841 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6901 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6961 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     7021 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7081 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7141 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7201 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7261 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7321 tctgacagga gctcgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     7381 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     7441 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     7501 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     7561 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg agcctggcga
     7621 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     7681 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     7741 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     7801 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     7861 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     7921 ccagccacga tagccgcgct gcctcgtctt ggagttcatt cagggcaccg gacaggtcgg
     7981 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     8041 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     8101 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     8161 cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     8221 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     8281 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
     8341 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccgggcga
     8401 tcgatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc
     8461 aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt
     8521 ccgccatctc cagcagccgc acgcggcgca tcgggggggg ggtttcaatt caattcatca
     8581 tttttttttt attctttttt ttgatttcgg tttctttgaa atttttttga ttcggtaatc
     8641 tccgaacaga aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata
     8701 tgtagtgttg aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa
     8761 acctgcagga aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact
     8821 catcctagtc ctgttgctgc caagctattt aatatcatgc acgaaaagca aacaaacttg
     8881 tgtgcttcat tggatgttcg taccaccaag gaattactgg agttagttga agcattaggt
     8941 cccaaaattt gtttactaaa aacacatgtg gatatcttga ctgatttttc catggagggc
     9001 acagttaagc cgctaaaggc attatccgcc aagtacaatt ttttactctt cgaagacaga
     9061 aaatttgctg acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt atacagaata
     9121 gcagaatggg cagacattac gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt
     9181 ttgaagcagg cggcagaaga agtaacaaag gaacctagag gccttttgat gttagcagaa
     9241 ttgtcatgca agggctccct atctactgga gaatatacta agggtactgt tgacattgcg
     9301 aagagcgaca aagattttgt tatcggcttt attgctcaaa gagacatggg tggaagagat
     9361 gaaggttacg attggttgat tatgacaccc ggtgtgggtt tagatgacaa gggagacgca
     9421 ttgggtcaac agtatagaac cgtggatgat gtggtctcta caggatctga cattattatt
     9481 gttggaagag gactatttgc aaagggaagg gatgctaagg tagagggtga acgttacaga
     9541 aaagcaggct gggaagcata tttgagaaga tgcggccagc aaaactaaaa aactgtatta
     9601 taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt atatcagtta
     9661 ttacccggga atctcggtcg taatgatttt tataatgacg aaaaaaaaaa aattggaaag
     9721 aaaacccccc ccccgcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
     9781 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
     9841 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
     9901 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
     9961 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
    10021 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
    10081 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac
    10141 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaattc
    10201 ccccttacac ggaggcatca agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
    10261 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
    10321 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg caggtgggcc
    10381 attctcatga agaatatctt gaatttattg tcatatta
//
