LOCUS       Exported               13051 bp DNA     circular SYN 27-SEP-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
COMMENT     SECID/File created by Clone Manager, Scientific
FEATURES             Location/Qualifiers
     source          1..13051
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        104..155
                     /label=P mob
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /note="/SECDescr=mob promoter including oriT (Szpirer et al
                     2001, PMID: 11222611)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P mob"
                     /note="/SECStyleId=1"
     CDS             223..1242
                     /codon_start=1
                     /transl_table=11
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /label=mob
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID: 
                     19928068328)."
                     /note="/SECDescr=Mob protein necessary for mobilization"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=mob"
                     /note="/SECStyleId=1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     misc_feature    1243..1742
                     /label=LP-L
                     /note="left side of integron landing pad sequence"
                     /note="/SECDescr=left side of integron landing pad 
                     sequence"
                     /note="/SECDrawAs=Region"
                     /note="/SECName=LP-L"
                     /note="/SECStyleId=1"
     misc_feature    1743..1782
                     /label=Core_up
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /note="/SECDescr=upstream sequence of YT_core which can be 
                     extracted with I-PpoI."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=Core_up"
     misc_feature    1783..1797
                     /label=I-PpoI
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-PpoI, enabling extraction of YT_core."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-PpoI"
     misc_feature    1798..1837
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /note="/SECDescr=upstream sequence of promoter slot 
                     (AsiSI/PI-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=P_up"
     misc_feature    1838..1874
                     /label=PI-SceI
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     PI-SceI, enabling e.g. promoter insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=PI-SceI"
     misc_feature    1875..1882
                     /label=AsiSI
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /note="/SECDescr=recognition site of endonuclease AsiSI, 
                     enabling e.g. promoter insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=AsiSI"
     misc_feature    1883..2002
                     /label=CIS_up
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /note="/SECDescr=upstream sequence of Cluster Integration 
                     Site (I-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=CIS_up"
     misc_feature    1883..1922
                     /label=P_dn
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /note="/SECDescr=downstream sequence of promoter slot 
                     (AsiSI/PI-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=P_dn"
     misc_feature    2003..2020
                     /label=I-SceI
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-SceI, enabling target gene insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-SceI"
     misc_feature    2021..2140
                     /label=CIS_dn
                     /note="downstream sequence of Cluster Integration Site 
                     (I-SceI)."
                     /note="/SECDescr=downstream sequence of Cluster Integration
                     Site (I-SceI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=CIS_dn"
     misc_feature    2101..2140
                     /gene="R_up"
                     /label=R_up
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /note="/SECDescr=upstream sequence of reporter slot 
                     (MauBI/I-CeuI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=R_up"
     misc_feature    2141..2148
                     /label=MauBI
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /note="/SECDescr=recognition site of endonuclease MauBI, 
                     enabling e.g. reporter insertion/exchange."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=MauBI"
     CDS             2170..2889
                     /codon_start=1
                     /transl_table=11
                     /gene="eYFP"
                     /product="yellow fluorescent protein eYFP"
                     /label=eYFP
                     /note="yellow fluorescent protein from Aequorea victoria
                     (Ormo et al. 1996, PMID: 8703075; Spiess et al. 2005, 
                     PMID:15725123)."
                     /note="/SECDescr=yellow fluorescent protein eYFP"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=eYFP"
                     /note="/SECStyleId=1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    2890..2897
                     /label=MauBI
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /note="/SECDescr=recognition site of endonuclease MauBI, 
                     enabling e.g. reporter insertion/exchange."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=MauBI"
     misc_feature    2898..2923
                     /label=I-CeuI
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion/exchange."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-CeuI, enabling e.g. reporter insertion/exchange."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-CeuI"
     misc_feature    2924..2963
                     /label=R_dn
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /note="/SECDescr=downstream sequence of reporter slot 
                     (MauBI/I-CeuI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=R_dn"
     misc_feature    2963..3020
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /label=MCS Integration site
                     /note="/SECDrawAs=Info only"
     misc_feature    2964..3019
                     /label=MC
                     /note="region with Multiple Cutters 
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon 
                     Tn5 insertion site elucidation."
                     /note="/SECDescr=region with Multiple Cutters 
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon 
                     Tn5 insertion site elucidation."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=MC"
     misc_feature    3020..3059
                     /label=M_up
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /note="/SECDescr=upstream sequence of marker slot 
                     (SalI/PI-PspI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=M_up"
     misc_feature    3060..3119
                     /label=P tet
                     /note="tet promoter region (Harley et al 1988, PMID:
                     3045754)."
                     /note="/SECDescr=tet promoter region (Harley et al 1988, 
                     PMID: 3045754)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P tet"
                     /note="/SECStyleId=1"
     CDS             3142..4332
                     /codon_start=1
                     /transl_table=11
                     /gene="tet"
                     /product="tetracycline effux pump"
                     /label=tet
                     /note="tet gene, TcR from pBR322 (Bolivar 1977, PMID:
                     344137; Sutcliffe 1979, PMID: 383387)."
                     /note="/SECDescr=tetracycline effux pump"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=tet"
                     /note="/SECStyleId=1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     misc_feature    4333..4338
                     /label=SalI
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /note="/SECDescr=recognition site of endonuclease SalI, 
                     enabling e.g. marker insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=SalI"
     misc_feature    4339..4368
                     /label=PI-PspI
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     PI-PspI, enabling e.g. marker insertion."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=PI-PspI"
     misc_feature    4369..4408
                     /label=M_dn
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /note="/SECDescr=downstream sequence of marker slot 
                     (SalI/PI-PspI)."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=M_dn"
     misc_feature    4409..4423
                     /label=I-PpoI
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /note="/SECDescr=recognition site of homing endonuclease 
                     I-PpoI, enabling extraction of YT_core."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=I-PpoI"
     misc_feature    4424..4463
                     /label=Core_dn
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /note="/SECDescr=downstream sequence of YT_core which can 
                     be extracted with I-PpoI."
                     /note="/SECDrawAs=Label"
                     /note="/SECName=Core_dn"
     misc_feature    4464..4963
                     /label=LP-R
                     /note="right side of integron landing pad sequence"
                     /note="/SECDescr=right side of integron landing pad 
                     sequence"
                     /note="/SECDrawAs=Region"
                     /note="/SECName=LP-R"
                     /note="/SECStyleId=1"
     misc_feature    5463..5573
                     /label=CEN4
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /note="/SECDescr=CEN4 of Saccharomyces cerevisiae Chr IV 
                     (Saccharomyces Genome Database)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=CEN4"
                     /note="/SECStyleId=1"
     misc_feature    6105..6849
                     /label=ARS1
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /note="/SECDescr=ARS1 of yCP50 (Genbank: X70276.1)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=ARS1 "
                     /note="/SECStyleId=1"
     rep_origin      7319..7907
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); 
                     annotated as New England Biolabs (2007)."
                     /note="/SECDescr=origin of replication from plasmid pBR322 
                     (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 
                     383387); annotated as New England Biolabs (2007)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=pMB1 ori"
                     /note="/SECStyleId=1"
     CDS             complement(8086..8880)
                     /codon_start=1
                     /transl_table=11
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /label=aphII
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin 
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /note="/SECDescr=aminoglycoside 3'-phosphotransferase"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=aphII"
                     /note="/SECStyleId=1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        8970..9018
                     /label=P aphII
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank 
                     U00004.1)."
                     /note="/SECDescr=promoter of aphII gene in Transposon Tn5 
                     (Genbank U00004.1)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P aphII"
                     /note="/SECStyleId=1"
     promoter        9318..9533
                     /label=P URA3
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /note="/SECDescr=URA3 promoter region (Roy et al 1990, 
                     PMID: 2204810)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P URA3"
                     /note="/SECStyleId=1"
     CDS             9534..10337
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /label=URA3
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /note="/SECDescr=orotidine 5'-phosphate decarboxylase"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=URA3"
                     /note="/SECStyleId=1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    11178..11628
                     /label=P sacB
                     /note="5'UTR of sacB incl. promoter and RBS (Steinmetz et
                     al 1985, PMID: 2993818)."
                     /note="/SECDescr=5'UTR of sacB incl. promoter and RBS 
                     (Steinmetz et al 1985, PMID: 2993818)."
                     /note="/SECDrawAs=Region"
                     /note="/SECName=P sacB"
                     /note="/SECStyleId=1"
     CDS             11629..13050
                     /codon_start=1
                     /transl_table=11
                     /gene="sacB"
                     /product="levan sucrase"
                     /label=sacB
                     /note="levan sucrase from Bacillus subtilis (Steinmetz et
                     al 1985, PMID: 2993818)"
                     /note="/SECDescr=levan sucrase"
                     /note="/SECDrawAs=Gene"
                     /note="/SECName=sacB"
                     /note="/SECStyleId=1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gagacgccac gcgatcgaac
     1261 gtcgttaact ctggcaggca attaaaggga acgtgtatct gacgcaaaga aaactggaaa
     1321 ttggcgagag aatcttctct ctgtctatcg aagactgacc acgcggaggc gtgcgtcttg
     1381 ttagcgtgcg gggtactctt gctatccatt tgagtcacac gacactcgct gttctcgact
     1441 ttacacttgt aagcgacggt attgaacgac gcttctgccc accatcgtta caaccagact
     1501 gatactagat ctatactgtc agccattcag acgaaaccag ccggagatta ccgagcactc
     1561 tattacgtcg gcgaccacta ctgagctagt ggagccgtgg gtaacgatca taccactaag
     1621 aacctctctc gacgcaagcg attacactgc tgtcacatca taatcgtctt gctattcacg
     1681 agttgaccag acactggata gcttgtcact tgaagtattc tgcacgacag ggtgcgtgta
     1741 cctatctgca gcactcaaca gatctccgtc tacttgcgac acctctctta aggtagctgg
     1801 accggattcg ggtctagcct atatcgtcaa tccgttgatc tatgtcgggt gcggagaaag
     1861 aggtaatgaa atgggcgatc gcccacagct tacacagacc taatcgtaga gttcctagta
     1921 atacttagtg gctcgttacc tcgttcttcc aggccagggg gccgtacgcg ctcggcgaac
     1981 agtataagca gattcgactc actagggata acagggtaat ctctgggtat cgactcaatc
     2041 tcgcgctatt gtgctatatc cgtggacggt tgcggtcaat tctgtcggaa aatcaacgga
     2101 tacgaacaat tactcgcgac ttcctcaata cgtgctgcaa cgcgcgcgaa ctttagaagg
     2161 agatatacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2221 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2281 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2341 tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac
     2401 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2461 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2521 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2581 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2641 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2701 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2761 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2821 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2881 tacaagtaac gcgcgcgtaa ctataacggt cctaaggtag cgagcttact tgtgctgttg
     2941 tgtaaggaca cgcttaaccc agagaattcc agcgacctat tctacgacta cgcgtccatg
     3001 gctcgaggag ctcggtaccg ccccactacc cgatttatca agatttggcg gagagtccct
     3061 catgtttgac agcttatcat cgataagctt taatgcggta gtttatcaca gttaaattgc
     3121 taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
     3181 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
     3241 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
     3301 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
     3361 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
     3421 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     3481 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     3541 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     3601 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     3661 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
     3721 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     3781 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     3841 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     3901 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     3961 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     4021 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     4081 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     4141 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     4201 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     4261 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     4321 acctcgacct gagtcgactg gcaaacagct attatgggta ttatgggtgt gtatccagaa
     4381 cgacggatct tgacgcaatt tggcgtggct ctcttaaggt agccctccga ccaattgagg
     4441 tgcgctctga ccgatcagtt tagggtgagc caacccggcc gatccggaga gacgggcctc
     4501 aaagccgcgt gacgacggct gtgggcccgt aacaaaatcc ccgcaataag ctcccgtgag
     4561 cgtcggttga acagccctgg tcggccccat cagtagcccg aatacttcgc tttacgggtc
     4621 ctgggccggg gtgcgatacc ttgcagaaat cgaggccgtt cgttaattcc tgttgcattc
     4681 gtaccgccta tatttgtctc tttgccggct tatcatcaca agcatagcat agccatttat
     4741 cggagcgcct ccgtacacgg tatgatcgga cgcctcgtga gatcaatacg tataccaggt
     4801 gtcctgtgag cagcgaaagc ctatacgcga gatacactgc caaaaatccg cgtgattacg
     4861 agtcgtggca aatttggtct ggctgtggtc tagacattcc aggcggtgcg tctgctctcg
     4921 ggtgcctcta gtggctggct agatagacta gccgctggta aactggtgtg gaagtccata
     4981 tatcggtgat caatatagtg gttgacatgc tggctagtca acattgagcc ttttgatcat
     5041 gcaaatatat tacggtattt tacaatcaaa tatcaaactt aactattgac tttataactt
     5101 atttaggtgg taacattctt ataaaaaaga aaaaaattac tgcaaaacag tactagcttt
     5161 taacttgtat cctaggttat ctatgctgtc tcaccataga gaatattacc tatttcagaa
     5221 tgtatgtcca tgattcgccg ggtaaataca tataatacac aaatctggct taataaagtc
     5281 tataatatat ctcataaaga agtgctaaat tggctagtgc tatatatttt taagaaaatt
     5341 tcttttgact aagtccatat cgactttgta aaagttcact ttagcataca tatattacac
     5401 gagccagaaa ttgtaacttt tgcctaaaat cacaaattgc aaaatttaat tgcttgcaaa
     5461 aggtcacatg cttataatca acttttttaa aaatttaaaa tactttttta ttttttattt
     5521 ttaaacataa atgaaataat ttatttattg tttatgatta ccgaaacata aaacctgctc
     5581 aagaaaaaga aactgttttg tccttggaaa aaaagcacta cctaggagcg gccaaaatgc
     5641 cgaggctttc atagcttaaa ctctttacag aaaataggca ttatagatca gttcgagttt
     5701 tcttattctt ccttccggtt ttatcgtcac agttttacag taaataagta tcacctctta
     5761 gagttaacta tgagataagc aagtatcatc tcatttcatt tacctgaagt cgagtaaaca
     5821 gaaaatccaa ttgttgatga acctcaatga cttagaacta tctatcggca gatcatataa
     5881 agaggattta ggtactcgag aacgctccgt acatgcagct aaacctccaa gcatctctaa
     5941 cttgggtagt gctttctcca ccattttctg tgcttgctcc ttcgtggcaa gtccagccca
     6001 taatgcccag aatgtagttg cggattcgta tgacgttctg tgcttgattt ttgtgttgta
     6061 gtcaaagaaa aaccccgact cgtcatccca catatatttg gtaattgatg aggcaacgct
     6121 aattatcaac atatagattg ttatctatct gcatgaacac gaaatcttta cttgacgact
     6181 tgaggctgat ggtgtttatg caaagaaacc actgtgttta atatgtgtca ctgtttgata
     6241 ttactgtcag cgtagaagat aatagtaaaa gcggttaata agtgtatttg agataagtgt
     6301 gataaagttt ttacagcgaa aagacgataa atacaagaaa atgattacga ggatacggag
     6361 agaggtatgt acatgtgtat ttatatacta agctgccggc ggttgtttgc aagaccgaga
     6421 aaaggctagc aagaatcggg tcattgtagc gtatgcgcct gtgaacattc tcttcaacaa
     6481 gtttgattcc attgcggtga aatggtaaaa gtcaaccccc tgcgatgtat attttcctgt
     6541 acaatcaatc aaaaagccaa atgatttagc attatcttta catcttgtta ttttacagat
     6601 tttatgttta gatcttttat gcttgctttt caaaaggcct gcaggcaagt gcacaaacaa
     6661 tacttaaata aatactactc agtaataacc tatttcttag catttttgac gaaatttgct
     6721 attttgttag agtcttttac accatttgtc tccacacctc cgcttacatc aacaccaata
     6781 acgccattta atctaagcgc atcaccaaca ttttctggcg tcagtccacc agctaacata
     6841 aaatgtaagc tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     6901 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     6961 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     7021 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     7081 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     7141 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7201 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7741 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8041 caatctaaag tatatatgag taaacttggt ctgacaggag ctcgctcaga agaactcgtc
     8101 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     8161 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     8221 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     8281 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc
     8341 gtcgggcatc cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     8401 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     8461 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     8521 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     8581 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     8641 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg
     8701 gagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     8761 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     8821 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     8881 gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc atcagatcct
     8941 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
     9001 cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca
     9061 tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat
     9121 agcccagtag ctgacattca tccgggcgat cgatggaagc cggcggcacc tcgctaacgg
     9181 attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac
     9241 caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat
     9301 cggggggggg gtttcaattc aattcatcat ttttttttta ttcttttttt tgatttcggt
     9361 ttctttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc
     9421 acagacttag attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag
     9481 tattcttaac ccaactgcac agaacaaaaa cctgcaggaa acgaagataa atcatgtcga
     9541 aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta
     9601 atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg
     9661 aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg
     9721 atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca
     9781 agtacaattt tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat
     9841 tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg
     9901 gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg
     9961 aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag
    10021 aatatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta
    10081 ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg
    10141 gtgtgggttt agatgacaag ggagacgcat tgggtcaaca gtatagaacc gtggatgatg
    10201 tggtctctac aggatctgac attattattg ttggaagagg actatttgca aagggaaggg
    10261 atgctaaggt agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat
    10321 gcggccagca aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa
    10381 ttagagcttc aatttaatta tatcagttat tacccgggaa tctcggtcgt aatgattttt
    10441 ataatgacga aaaaaaaaaa attggaaaga aaaccccccc cccgcagcgt tgggtcctgg
    10501 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
    10561 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
    10621 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
    10681 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc
    10741 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga
    10801 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc
    10861 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc
    10921 ggtatcatta cccccatgaa cagaaattcc cccttacacg gaggcatcaa gtgaccaaac
    10981 aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg
    11041 agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc
    11101 acgctgatga gctttaccgc aggtgggcca ttctcatgaa gaatatcttg aatttattgt
    11161 catattacta gcacgtgccc atcacatata cctgccgttc actattattt agtgaaatga
    11221 gatattatga tattttctga attgtgatta aaaaggcaac tttatgccca tgcaacagaa
    11281 actataaaaa atacagagaa tgaaaagaaa cagatagatt ttttagttct ttaggcccgt
    11341 agtctgcaaa tccttttatg attttctatc aaacaaaaga ggaaaataga ccagttgcaa
    11401 tccaaacgag agtctaatag aatgaggtcg aaaagtaaat cgcgcgggtt tgttactgat
    11461 aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta tactttggcg tcacccctta
    11521 catattttag gtcttttttt attgtgcgta actaacttgc catcttcaaa caggagggct
    11581 ggaagaagca gaccgctaac acagtacata aaaaaggaga catgaacgat gaacatcaaa
    11641 aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc aggaggcgca
    11701 actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata cggcatttcc
    11761 catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga aaaatatcaa
    11821 gtttctgaat ttgattcgtc cacaattaaa aatatctctt ctgcaaaagg cctggacgtt
    11881 tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca cggctaccac
    11941 atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat ttacatgttc
    12001 tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg cgtctttaaa
    12061 gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca agaatggtca
    12121 ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga tttctccggt
    12181 aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc atcagacagc
    12241 tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga cggaaaaacg
    12301 tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga caaccatacg
    12361 ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt tgaagcaaac
    12421 actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc atactatggc
    12481 aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga taaaaaacgc
    12541 acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga ttacacactg
    12601 aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat tgaacgcgcg
    12661 aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg atcaaaaatg
    12721 acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc taattcttta
    12781 actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga tcttgatcct
    12841 aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg aaacaatgtc
    12901 gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc aacgtttgcg
    12961 ccgagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga cagcatcctt
    13021 gaacaaggac aattaacagt taacaaataa a
//
