LOCUS       pYTRW27K_7Ti1          15406 bp    DNA     circular SYN 27-SEP-2021
DEFINITION  
ACCESSION   pYTRW27K_7Ti1
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     promoter        104..155
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /label=P mob
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P mob"
                     /SECDescr="mob promoter including oriT (Szpirer et al 2001, PMID: 11222611)."
     CDS             223..1242
                     /gene="mob"
                     /product="Mob protein necessary for mobilization"
                     /note="Mob as encoded in pBBR1MCS (Antoine & Locht PMID:
                     19928068328)."
                     /transl_table=11
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mob"
                     /SECDescr="Mob protein necessary for mobilization"
     misc_feature    1243..1742
                     /label=LP-L
                     /note="left side of integron landing pad sequence"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LP-L"
                     /SECDescr="left side of integron landing pad sequence"
     misc_feature    1743..1782
                     /note="upstream sequence of YT_core which can be extracted
                     with I-PpoI."
                     /label=Core_up
                     /SECDrawAs="Label"
                     /SECName="Core_up"
                     /SECDescr="upstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    1783..1797
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    1798..1837
                     /note="upstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /SECDrawAs="Label"
                     /SECName="P_up"
                     /SECDescr="upstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1838..1874
                     /note="recognition site of homing endonuclease PI-SceI,
                     enabling e.g. promoter insertion."
                     /label=PI-SceI
                     /SECDrawAs="Label"
                     /SECName="PI-SceI"
                     /SECDescr="recognition site of homing endonuclease PI-SceI, enabling e.g. promoter insertion."
     misc_feature    1875..1882
                     /note="recognition site of endonuclease AsiSI, enabling
                     e.g. promoter insertion."
                     /label=AsiSI
                     /SECDrawAs="Label"
                     /SECName="AsiSI"
                     /SECDescr="recognition site of endonuclease AsiSI, enabling e.g. promoter insertion."
     misc_feature    1883..1922
                     /note="downstream sequence of promoter slot
                     (AsiSI/PI-SceI)."
                     /label=P_dn
                     /SECDrawAs="Label"
                     /SECName="P_dn"
                     /SECDescr="downstream sequence of promoter slot (AsiSI/PI-SceI)."
     misc_feature    1883..2002
                     /note="upstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_up
                     /SECDrawAs="Label"
                     /SECName="CIS_up"
                     /SECDescr="upstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    2003..2020
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /label=I-SceI
                     /SECDrawAs="Label"
                     /SECName="I-SceI"
                     /SECDescr="recognition site of homing endonuclease I-SceI, enabling target gene insertion."
     misc_feature    2021..2140
                     /note="downstream sequence of Cluster Integration Site
                     (I-SceI)."
                     /label=CIS_dn
                     /SECDrawAs="Label"
                     /SECName="CIS_dn"
                     /SECDescr="downstream sequence of Cluster Integration Site (I-SceI)."
     misc_feature    2101..2140
                     /note="upstream sequence of reporter slot (MauBI/I-CeuI)."
                     /gene="R_up"
                     /SECDrawAs="Label"
                     /SECName="R_up"
                     /SECDescr="upstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    2141..2148
                     /label=MauBI
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion/exchange."
     CDS             2170..5244
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /transl_table=11
                     /note="lacZ from Escherichia coli K12 (Kalnins et al.
                     1983, PMID: 6313347)."
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="lacZ"
                     /SECDescr="beta-galactosidase"
     misc_feature    5245..5252
                     /note="recognition site of endonuclease MauBI, enabling
                     e.g. reporter insertion/exchange."
                     /label=MauBI
                     /SECDrawAs="Label"
                     /SECName="MauBI"
                     /SECDescr="recognition site of endonuclease MauBI, enabling e.g. reporter insertion/exchange."
     misc_feature    5253..5278
                     /note="recognition site of homing endonuclease I-CeuI,
                     enabling e.g. reporter insertion/exchange."
                     /label=I-CeuI
                     /SECDrawAs="Label"
                     /SECName="I-CeuI"
                     /SECDescr="recognition site of homing endonuclease I-CeuI, enabling e.g. reporter insertion/exchange."
     misc_feature    5279..5318
                     /note="downstream sequence of reporter slot
                     (MauBI/I-CeuI)."
                     /label=R_dn
                     /SECDrawAs="Label"
                     /SECName="R_dn"
                     /SECDescr="downstream sequence of reporter slot (MauBI/I-CeuI)."
     misc_feature    5318..5375
                     /label=MCS Integration site
                     /product="6 Schnittstellen, 5 verschachtelt 1 etwas aussen
                     links 20 bp vor MCS"
                     /SECDrawAs="Info only"
     misc_feature    5319..5374
                     /note="region with Multiple Cutters
                     EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon
                     Tn5 insertion site elucidation."
                     /label=MC
                     /SECDrawAs="Label"
                     /SECName="MC"
                     /SECDescr="region with Multiple Cutters EcoRI,MluI,NcoI,XhoI,SacI,KpnI, enabling e.g. transposon Tn5 insertion site elucidation."
     misc_feature    5375..5414
                     /note="upstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_up
                     /SECDrawAs="Label"
                     /SECName="M_up"
                     /SECDescr="upstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    5415..5474
                     /label=P tet
                     /note="tet promoter region (Harley et al 1988, PMID:
                     3045754)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tet"
                     /SECDescr="tet promoter region (Harley et al 1988, PMID: 3045754)."
     CDS             5497..6687
                     /gene="tet"
                     /product="tetracycline effux pump"
                     /transl_table=11
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
                     /note="tet gene, TcR from pBR322 (Bolivar 1977, PMID:
                     344137; Sutcliffe 1979, PMID: 383387)."
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tet"
                     /SECDescr="tetracycline effux pump"
     misc_feature    6688..6693
                     /note="recognition site of endonuclease SalI, enabling
                     e.g. marker insertion."
                     /label=SalI
                     /SECDrawAs="Label"
                     /SECName="SalI"
                     /SECDescr="recognition site of endonuclease SalI, enabling e.g. marker insertion."
     misc_feature    6694..6723
                     /note="recognition site of homing endonuclease PI-PspI,
                     enabling e.g. marker insertion."
                     /label=PI-PspI
                     /SECDrawAs="Label"
                     /SECName="PI-PspI"
                     /SECDescr="recognition site of homing endonuclease PI-PspI, enabling e.g. marker insertion."
     misc_feature    6724..6763
                     /note="downstream sequence of marker slot (SalI/PI-PspI)."
                     /label=M_dn
                     /SECDrawAs="Label"
                     /SECName="M_dn"
                     /SECDescr="downstream sequence of marker slot (SalI/PI-PspI)."
     misc_feature    6764..6778
                     /note="recognition site of homing endonuclease I-PpoI,
                     enabling extraction of YT_core."
                     /label=I-PpoI
                     /SECDrawAs="Label"
                     /SECName="I-PpoI"
                     /SECDescr="recognition site of homing endonuclease I-PpoI, enabling extraction of YT_core."
     misc_feature    6779..6818
                     /note="downstream sequence of YT_core which can be
                     extracted with I-PpoI."
                     /label=Core_dn
                     /SECDrawAs="Label"
                     /SECName="Core_dn"
                     /SECDescr="downstream sequence of YT_core which can be extracted with I-PpoI."
     misc_feature    6819..7318
                     /label=LP-R
                     /note="right side of integron landing pad sequence"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LP-R"
                     /SECDescr="right side of integron landing pad sequence"
     misc_feature    7818..7928
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /label=CEN4
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="CEN4"
                     /SECDescr="CEN4 of Saccharomyces cerevisiae Chr IV (Saccharomyces Genome Database)."
     misc_feature    8460..9204
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /label=ARS1
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ARS1 "
                     /SECDescr="ARS1 of yCP50 (Genbank: X70276.1)."
     rep_origin      9674..10262
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387);
                     annotated as New England Biolabs (2007)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pMB1 ori"
                     /SECDescr="origin of replication from plasmid pBR322 (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); annotated as New England Biolabs (2007)."
     CDS             complement(10441..11235)
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /transl_table=11
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aphII"
                     /SECDescr="aminoglycoside 3'-phosphotransferase"
     promoter        11325..11373
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank
                     U00004.1)."
                     /label=P aphII
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aphII"
                     /SECDescr="promoter of aphII gene in Transposon Tn5 (Genbank U00004.1)."
     promoter        11673..11888
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /label=P URA3
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P URA3"
                     /SECDescr="URA3 promoter region (Roy et al 1990, PMID: 2204810)."
     CDS             11889..12692
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="URA3"
                     /SECDescr="orotidine 5'-phosphate decarboxylase"
     misc_feature    13533..13983
                     /label=P sacB
                     /note="5'UTR of sacB incl. promoter and RBS (Steinmetz et
                     al 1985, PMID: 2993818)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P sacB"
                     /SECDescr="5'UTR of sacB incl. promoter and RBS (Steinmetz et al 1985, PMID: 2993818)."
     CDS             13984..15405
                     /gene="sacB"
                     /product="levan sucrase"
                     /transl_table=11
                     /note="levan sucrase from Bacillus subtilis (Steinmetz et
                     al 1985, PMID: 2993818)"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGIS
                     HITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHG
                     YHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQT
                     QEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIF
                     DGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEES
                     LFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIAS
                     NTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLN
                     KTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLN
                     IKGKKTSVVKDSILEQGQLTVNK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="sacB"
                     /SECDescr="levan sucrase"
ORIGIN      
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gagacgccac gcgatcgaac
     1261 gtcgttaact ctggcaggca attaaaggga acgtgtatct gacgcaaaga aaactggaaa
     1321 ttggcgagag aatcttctct ctgtctatcg aagactgacc acgcggaggc gtgcgtcttg
     1381 ttagcgtgcg gggtactctt gctatccatt tgagtcacac gacactcgct gttctcgact
     1441 ttacacttgt aagcgacggt attgaacgac gcttctgccc accatcgtta caaccagact
     1501 gatactagat ctatactgtc agccattcag acgaaaccag ccggagatta ccgagcactc
     1561 tattacgtcg gcgaccacta ctgagctagt ggagccgtgg gtaacgatca taccactaag
     1621 aacctctctc gacgcaagcg attacactgc tgtcacatca taatcgtctt gctattcacg
     1681 agttgaccag acactggata gcttgtcact tgaagtattc tgcacgacag ggtgcgtgta
     1741 cctatctgca gcactcaaca gatctccgtc tacttgcgac acctctctta aggtagctgg
     1801 accggattcg ggtctagcct atatcgtcaa tccgttgatc tatgtcgggt gcggagaaag
     1861 aggtaatgaa atgggcgatc gcccacagct tacacagacc taatcgtaga gttcctagta
     1921 atacttagtg gctcgttacc tcgttcttcc aggccagggg gccgtacgcg ctcggcgaac
     1981 agtataagca gattcgactc actagggata acagggtaat ctctgggtat cgactcaatc
     2041 tcgcgctatt gtgctatatc cgtggacggt tgcggtcaat tctgtcggaa aatcaacgga
     2101 tacgaacaat tactcgcgac ttcctcaata cgtgctgcaa cgcgcgcgaa ctttagaagg
     2161 agatatacca tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
     2221 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     2281 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2341 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc
     2401 gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat
     2461 gcgcccatct acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg
     2521 gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa
     2581 ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg
     2641 cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt
     2701 ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat
     2761 ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat
     2821 aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc
     2881 cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta
     2941 acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt
     3001 gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa
     3061 aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac
     3121 accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg
     3181 attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt
     3241 cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc
     3301 ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg
     3361 ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa
     3421 acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg
     3481 agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg
     3541 tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa
     3601 tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc
     3661 gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg
     3721 aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc
     3781 gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt
     3841 cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt
     3901 aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg
     3961 aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg
     4021 ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc
     4081 gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg
     4141 gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt
     4201 aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc
     4261 acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc
     4321 tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc
     4381 atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg
     4441 caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg
     4501 ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt
     4561 gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac
     4621 caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt
     4681 acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac
     4741 cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca
     4801 ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac
     4861 tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac
     4921 cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt
     4981 ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag
     5041 ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg
     5101 cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac
     5161 gactcctgga gcccgtcagt atcggcggaa ttacagctga gcgccggtcg ctaccattac
     5221 cagttggtct ggtgtcaaaa ataacgcgcg cgtaactata acggtcctaa ggtagcgagc
     5281 ttacttgtgc tgttgtgtaa ggacacgctt aacccagaga attccagcga cctattctac
     5341 gactacgcgt ccatggctcg aggagctcgg taccgcccca ctacccgatt tatcaagatt
     5401 tggcggagag tccctcatgt ttgacagctt atcatcgata agctttaatg cggtagttta
     5461 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
     5521 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
     5581 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
     5641 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
     5701 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
     5761 atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc
     5821 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     5881 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     5941 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     6001 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     6061 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     6121 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     6181 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     6241 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     6301 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     6361 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     6421 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     6481 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     6541 atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     6601 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     6661 gcatggagcc gggccacctc gacctgagtc gactggcaaa cagctattat gggtattatg
     6721 ggtgtgtatc cagaacgacg gatcttgacg caatttggcg tggctctctt aaggtagccc
     6781 tccgaccaat tgaggtgcgc tctgaccgat cagtttaggg tgagccaacc cggccgatcc
     6841 ggagagacgg gcctcaaagc cgcgtgacga cggctgtggg cccgtaacaa aatccccgca
     6901 ataagctccc gtgagcgtcg gttgaacagc cctggtcggc cccatcagta gcccgaatac
     6961 ttcgctttac gggtcctggg ccggggtgcg ataccttgca gaaatcgagg ccgttcgtta
     7021 attcctgttg cattcgtacc gcctatattt gtctctttgc cggcttatca tcacaagcat
     7081 agcatagcca tttatcggag cgcctccgta cacggtatga tcggacgcct cgtgagatca
     7141 atacgtatac caggtgtcct gtgagcagcg aaagcctata cgcgagatac actgccaaaa
     7201 atccgcgtga ttacgagtcg tggcaaattt ggtctggctg tggtctagac attccaggcg
     7261 gtgcgtctgc tctcgggtgc ctctagtggc tggctagata gactagccgc tggtaaactg
     7321 gtgtggaagt ccatatatcg gtgatcaata tagtggttga catgctggct agtcaacatt
     7381 gagccttttg atcatgcaaa tatattacgg tattttacaa tcaaatatca aacttaacta
     7441 ttgactttat aacttattta ggtggtaaca ttcttataaa aaagaaaaaa attactgcaa
     7501 aacagtacta gcttttaact tgtatcctag gttatctatg ctgtctcacc atagagaata
     7561 ttacctattt cagaatgtat gtccatgatt cgccgggtaa atacatataa tacacaaatc
     7621 tggcttaata aagtctataa tatatctcat aaagaagtgc taaattggct agtgctatat
     7681 atttttaaga aaatttcttt tgactaagtc catatcgact ttgtaaaagt tcactttagc
     7741 atacatatat tacacgagcc agaaattgta acttttgcct aaaatcacaa attgcaaaat
     7801 ttaattgctt gcaaaaggtc acatgcttat aatcaacttt tttaaaaatt taaaatactt
     7861 ttttattttt tatttttaaa cataaatgaa ataatttatt tattgtttat gattaccgaa
     7921 acataaaacc tgctcaagaa aaagaaactg ttttgtcctt ggaaaaaaag cactacctag
     7981 gagcggccaa aatgccgagg ctttcatagc ttaaactctt tacagaaaat aggcattata
     8041 gatcagttcg agttttctta ttcttccttc cggttttatc gtcacagttt tacagtaaat
     8101 aagtatcacc tcttagagtt aactatgaga taagcaagta tcatctcatt tcatttacct
     8161 gaagtcgagt aaacagaaaa tccaattgtt gatgaacctc aatgacttag aactatctat
     8221 cggcagatca tataaagagg atttaggtac tcgagaacgc tccgtacatg cagctaaacc
     8281 tccaagcatc tctaacttgg gtagtgcttt ctccaccatt ttctgtgctt gctccttcgt
     8341 ggcaagtcca gcccataatg cccagaatgt agttgcggat tcgtatgacg ttctgtgctt
     8401 gatttttgtg ttgtagtcaa agaaaaaccc cgactcgtca tcccacatat atttggtaat
     8461 tgatgaggca acgctaatta tcaacatata gattgttatc tatctgcatg aacacgaaat
     8521 ctttacttga cgacttgagg ctgatggtgt ttatgcaaag aaaccactgt gtttaatatg
     8581 tgtcactgtt tgatattact gtcagcgtag aagataatag taaaagcggt taataagtgt
     8641 atttgagata agtgtgataa agtttttaca gcgaaaagac gataaataca agaaaatgat
     8701 tacgaggata cggagagagg tatgtacatg tgtatttata tactaagctg ccggcggttg
     8761 tttgcaagac cgagaaaagg ctagcaagaa tcgggtcatt gtagcgtatg cgcctgtgaa
     8821 cattctcttc aacaagtttg attccattgc ggtgaaatgg taaaagtcaa ccccctgcga
     8881 tgtatatttt cctgtacaat caatcaaaaa gccaaatgat ttagcattat ctttacatct
     8941 tgttatttta cagattttat gtttagatct tttatgcttg cttttcaaaa ggcctgcagg
     9001 caagtgcaca aacaatactt aaataaatac tactcagtaa taacctattt cttagcattt
     9061 ttgacgaaat ttgctatttt gttagagtct tttacaccat ttgtctccac acctccgctt
     9121 acatcaacac caataacgcc atttaatcta agcgcatcac caacattttc tggcgtcagt
     9181 ccaccagcta acataaaatg taagctctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     9241 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     9301 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     9361 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     9421 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     9481 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9541 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9601 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9661 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9721 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9781 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9841 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9901 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9961 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10021 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10081 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    10141 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10201 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10261 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10321 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10381 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac aggagctcgc
    10441 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
    10501 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
    10561 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
    10621 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
    10681 gacgagatcc tcgccgtcgg gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc
    10741 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
    10801 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
    10861 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
    10921 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
    10981 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
    11041 cgctgcctcg tcttggagtt cattcagggc accggacagg tcggtcttga caaaaagaac
    11101 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
    11161 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
    11221 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct
    11281 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
    11341 cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca
    11401 gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt
    11461 ttcccttgtc cagatagccc agtagctgac attcatccgg gcgatcgatg gaagccggcg
    11521 gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac
    11581 tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag
    11641 ccgcacgcgg cgcatcgggg ggggggtttc aattcaattc atcatttttt ttttattctt
    11701 ttttttgatt tcggtttctt tgaaattttt ttgattcggt aatctccgaa cagaaggaag
    11761 aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa
    11821 catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa
    11881 gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg
    11941 ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg
    12001 ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac
    12061 taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa
    12121 aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg
    12181 gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca
    12241 ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag
    12301 aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct
    12361 ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt
    12421 ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt
    12481 tgattatgac acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata
    12541 gaaccgtgga tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat
    12601 ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag
    12661 catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta
    12721 tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc gggaatctcg
    12781 gtcgtaatga tttttataat gacgaaaaaa aaaaaattgg aaagaaaacc ccccccccgc
    12841 agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta
    12901 ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga
    12961 agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc
    13021 cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc
    13081 tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc
    13141 tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta
    13201 ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc
    13261 ctctctcgtt tcatcggtat cattaccccc atgaacagaa attccccctt acacggaggc
    13321 atcaagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
    13381 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
    13441 aatcgcttca cgaccacgct gatgagcttt accgcaggtg ggccattctc atgaagaata
    13501 tcttgaattt attgtcatat tactagcacg tgcccatcac atatacctgc cgttcactat
    13561 tatttagtga aatgagatat tatgatattt tctgaattgt gattaaaaag gcaactttat
    13621 gcccatgcaa cagaaactat aaaaaataca gagaatgaaa agaaacagat agatttttta
    13681 gttctttagg cccgtagtct gcaaatcctt ttatgatttt ctatcaaaca aaagaggaaa
    13741 atagaccagt tgcaatccaa acgagagtct aatagaatga ggtcgaaaag taaatcgcgc
    13801 gggtttgtta ctgataaagc aggcaagacc taaaatgtgt aaagggcaaa gtgtatactt
    13861 tggcgtcacc ccttacatat tttaggtctt tttttattgt gcgtaactaa cttgccatct
    13921 tcaaacagga gggctggaag aagcagaccg ctaacacagt acataaaaaa ggagacatga
    13981 acgatgaaca tcaaaaagtt tgcaaaacaa gcaacagtat taacctttac taccgcactg
    14041 ctggcaggag gcgcaactca agcgtttgcg aaagaaacga accaaaagcc atataaggaa
    14101 acatacggca tttcccatat tacacgccat gatatgctgc aaatccctga acagcaaaaa
    14161 aatgaaaaat atcaagtttc tgaatttgat tcgtccacaa ttaaaaatat ctcttctgca
    14221 aaaggcctgg acgtttggga cagctggcca ttacaaaacg ctgacggcac tgtcgcaaac
    14281 tatcacggct accacatcgt ctttgcatta gccggagatc ctaaaaatgc ggatgacaca
    14341 tcgatttaca tgttctatca aaaagtcggc gaaacttcta ttgacagctg gaaaaacgct
    14401 ggccgcgtct ttaaagacag cgacaaattc gatgcaaatg attctatcct aaaagaccaa
    14461 acacaagaat ggtcaggttc agccacattt acatctgacg gaaaaatccg tttattctac
    14521 actgatttct ccggtaaaca ttacggcaaa caaacactga caactgcaca agttaacgta
    14581 tcagcatcag acagctcttt gaacatcaac ggtgtagagg attataaatc aatctttgac
    14641 ggtgacggaa aaacgtatca aaatgtacag cagttcatcg atgaaggcaa ctacagctca
    14701 ggcgacaacc atacgctgag agatcctcac tacgtagaag ataaaggcca caaatactta
    14761 gtatttgaag caaacactgg aactgaagat ggctaccaag gcgaagaatc tttatttaac
    14821 aaagcatact atggcaaaag cacatcattc ttccgtcaag aaagtcaaaa acttctgcaa
    14881 agcgataaaa aacgcacggc tgagttagca aacggcgctc tcggtatgat tgagctaaac
    14941 gatgattaca cactgaaaaa agtgatgaaa ccgctgattg catctaacac agtaacagat
    15001 gaaattgaac gcgcgaacgt ctttaaaatg aacggcaaat ggtacctgtt cactgactcc
    15061 cgcggatcaa aaatgacgat tgacggcatt acgtctaacg atatttacat gcttggttat
    15121 gtttctaatt ctttaactgg cccatacaag ccgctgaaca aaactggcct tgtgttaaaa
    15181 atggatcttg atcctaacga tgtaaccttt acttactcac acttcgctgt acctcaagcg
    15241 aaaggaaaca atgtcgtgat tacaagctat atgacaaaca gaggattcta cgcagacaaa
    15301 caatcaacgt ttgcgccgag cttcctgctg aacatcaaag gcaagaaaac atctgttgtc
    15361 aaagacagca tccttgaaca aggacaatta acagttaaca aataaa
//
