LOCUS       yTREX                  10548 bp    DNA     circular SYN 27-SEP-2021
DEFINITION  
ACCESSION   yTREX
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10548)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     promoter        145..196
                     /label=P mob
                     /note="mob promoter including oriT (Szpirer et al 2001,
                     PMID: 11222611)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P mob"
                     /SECDescr="mob promoter including oriT (Szpirer et al 2001, PMID: 11222611)."
     CDS             264..1337
                     /gene="mob"
                     /product="Mob protein for mobilization"
                     /transl_table=11
                     /note="mob protein for mobilization, as encoded in
                     pBBR1MCS (Antoine & Locht PMID: 19928068328) up to E327."
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPELTLIHKC
                     SFLRYPLILWITVNSVSRLESGQ"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mob"
                     /SECDescr="Mob protein for mobilization"
     misc_feature    1245..1263
                     /label=OE-L
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-L"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     promoter        1350..1371
                     /label=P T7
                     /note="bacteriophage T7 promoter (Dunn & Studier 1983,
                     PMID: 6864790)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P T7"
                     /SECDescr="bacteriophage T7 promoter (Dunn & Studier 1983, PMID: 6864790)."
     misc_feature    1412..1429
                     /label=I-SceI
                     /note="recognition site of homing endonuclease I-SceI,
                     enabling target gene insertion."
                     /SECDrawAs="Label"
                     /SECName="I-SceI"
                     /SECDescr="recognition site of homing endonuclease I-SceI, enabling target gene insertion."
     promoter        complement(1470..1491)
                     /label=P T7
                     /note="bacteriophage T7 promoter (Dunn & Studier 1983,
                     PMID: 6864790)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P T7"
                     /SECDescr="bacteriophage T7 promoter (Dunn & Studier 1983, PMID: 6864790)."
     misc_feature    1538..1597
                     /label=P tet
                     /note="tet promoter region (Harley et al 1988, PMID:
                     3045754)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tet"
                     /SECDescr="tet promoter region (Harley et al 1988, PMID: 3045754)."
     CDS             1620..2810
                     /gene="tet"
                     /product="tetracycline efflux pump"
                     /transl_table=11
                     /note="tet gene, TcR from pBR322 (Bolivar 1977, PMID:
                     344137; Sutcliffe 1979, PMID: 383387)."
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tet"
                     /SECDescr="tetracycline efflux pump"
     misc_feature    complement(2851..2869)
                     /label=OE-R
                     /note="outside end sequence of transposon Tn5 (Reznikoff,
                     2008, PMID:18680433)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OE-R"
                     /SECDescr="outside end sequence of transposon Tn5 (Reznikoff, 2008, PMID:18680433)."
     promoter        2880..2910
                     /label=P tnp
                     /note="transposon Tn5 transposase promoter (Yin et al
                     1988, PMID: 2451025)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P tnp"
                     /SECDescr="transposon Tn5 transposase promoter (Yin et al 1988, PMID: 2451025)."
     CDS             2943..4373
                     /gene="tnp"
                     /product="Transposon Tn5 transposase"
                     /transl_table=11
                     /note="transposon Tn5 transposase (Goryshin & Reznikoff
                     1998, PMID: 9516433)."
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGK
                     SITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLS
                     YRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEK
                     ESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDV
                     ESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGN
                     ITLNAVLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKT
                     GAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQS
                     AETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW
                     EGWEALQSKLDGFLAAKDLMAQGIKI"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tnp"
                     /SECDescr="Transposon Tn5 transposase"
     misc_feature    4885..4995
                     /label=CEN4
                     /note="CEN4 of Saccharomyces cerevisiae Chr IV
                     (Saccharomyces Genome Database)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="CEN4"
                     /SECDescr="CEN4 of S.cerevisiae Chr IV (Saccharomyces Genome Database)"
     misc_feature    5527..6271
                     /label=ARS1
                     /note="ARS1 of yCP50 (Genbank: X70276.1)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ARS1"
                     /SECDescr="ARS1 of yCP50 (Genbank: X70276.1)."
     rep_origin      6741..7329
                     /label=ori pMB1
                     /note="origin of replication from plasmid pBR322 (Bolivar
                     et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387);
                     annotated as New England Biolabs (2007)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pMB1 ori"
                     /SECDescr="origin of replication from plasmid pBR322 (Bolivar et al 1977, PMID: 344137; Sutcliffe 1979, PMID: 383387); annotated as New England Biolabs (2007)."
     CDS             complement(7508..8302)
                     /gene="aphII"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /transl_table=11
                     /note="aminoglycoside 3'-phosphotransferase, kanamycin
                     resistance gene, KmR (Pridmore 1987, PMID: 3315864)."
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="aphII"
                     /SECDescr="aminoglycoside 3'-phosphotransferase"
     promoter        complement(8392..8440)
                     /label=P aphII
                     /note="promoter of aphII gene in Transposon Tn5 (Genbank
                     U00004.1)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P aphII"
                     /SECDescr="promoter of aphII gene in Transposon Tn5 (Genbank U00004.1)."
     promoter        8740..8955
                     /label=P URA3
                     /note="URA3 promoter region (Roy et al 1990, PMID:
                     2204810)."
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P URA3"
                     /SECDescr="URA3 promoter region (Roy et al 1990, PMID: 2204810)."
     CDS             8956..9759
                     /gene="URA3"
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /note="orotidine 5'-phosphate decarboxylase, part of
                     yCP50-poly backbone (Wang & Stillman, PMID: 8441414)."
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="URA3"
                     /SECDescr="orotidine 5'-phosphate decarboxylase"
ORIGIN      
        1 gccattctca tgaagaatat cttgaattta ttgtcatatt actagtcccg ggtccacata
       61 tccacgggct ggcaagggag cgcagcgacc gcgcagggcg aagcccggag agcaagcccg
      121 tagggcgccg cagccgccgt aggcggtcac gactttgcga agcaaagtct agtgagtata
      181 ctcaagcatt gagtggcccg ccggaggcac cgccttgcgc tgcccccgtc gagccggttg
      241 gacaccaaaa gggaggggca ggcatggcgg catacgcgat catgcgatgc aagaagctgg
      301 cgaaaatggg caacgtggcg gccagtctca agcacgccta ccgcgagcgc gagacgccca
      361 acgctgacgc cagcaggacg ccagagaacg agcactgggc ggccagcagc accgatgaag
      421 cgatgggccg actgcgcgag ttgctgccag agaagcggcg caaggacgct gtgttggcgg
      481 tcgagtacgt catgacggcc agcccggaat ggtggaagtc ggccagccaa gaacagcagg
      541 cggcgttctt cgagaaggcg cacaagtggc tggcggacaa gtacggggcg gatcgcatcg
      601 tgacggccag catccaccgt gacgaaacca gcccgcacat gaccgcgttc gtggtgccgc
      661 tgacgcagga cggcaggctg tcggccaagg agttcatcgg caacaaagcg cagatgaccc
      721 gcgaccagac cacgtttgcg gccgctgtgg ccgatctagg gctgcaacgg ggcatcgagg
      781 gcagcaaggc acgtcacacg cgcattcagg cgttctacga ggccctggag cggccaccag
      841 tgggccacgt caccatcagc ccgcaagcgg tcgagccacg cgcctatgca ccgcagggat
      901 tggccgaaaa gctgggaatc tcaaagcgcg ttgagacgcc ggaagccgtg gccgaccggc
      961 tgacaaaagc ggttcggcag gggtatgagc ctgccctaca ggccgccgca ggagcgcgtg
     1021 agatgcgcaa gaaggccgat caagcccaag agacggcccg agaccttcgg gagcgcctga
     1081 agcccgttct ggacgccctg gggccgttga atcgggatat gcaggccaag gccgccgcga
     1141 tcatcaaggc cgtgggcgaa aagctgctga cggaacagcg ggaagtccag cgccagaaac
     1201 aggcccagcg ccagcaggaa cgcgggcgcg cacatttccc cgaactgact cttatacaca
     1261 agtgttcttt cctgcgttat cccctgattc tgtggataac cgtgaattcg gtttcccgac
     1321 tggaaagcgg gcagtgagcg caacgcaata attaatacga ctcactatag gcctccaaac
     1381 tagaaatatt agctaattta atctctcaac ctagggataa cagggtaatt tggcgtaatc
     1441 atggtcatag ctgtttcctg tgtgaaattc ctatagtgag tcgtattaat taggttgggc
     1501 ttcggaatcg ttttccggga cgccggctgg agaattctca tgtttgacag cttatcatcg
     1561 ataagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta
     1621 tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca
     1681 taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca
     1741 tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac
     1801 ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac
     1861 ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atcctctacg
     1921 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     1981 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     2041 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     2101 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     2161 tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca
     2221 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     2281 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     2341 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     2401 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     2461 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     2521 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     2581 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     2641 cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg atttatgccg
     2701 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
     2761 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga agagcgccca
     2821 atacgcaaac cgcctctccc cgcgcgttgg acttgtgtat aagagtcaga gcgtcctgaa
     2881 cggaaccttt cccgttttcc aggatctgat cttccatgtg acctcctaac atggtaacgt
     2941 tcatgataac ttctgctctt catcgtgcgg ccgactgggc taaatctgtg ttctcttcgg
     3001 cggcgctggg tgatcctcgc cgtactgccc gcttggttaa cgtcgccgcc caattggcaa
     3061 aatattctgg taaatcaata accatctcat cagagggtag tgaagccatg caggaaggcg
     3121 cttaccgatt tatccgcaat cccaacgttt ctgccgaggc gatcagaaag gctggcgcca
     3181 tgcaaacagt caagttggct caggagtttc ccgaactgct ggccattgag gacaccacct
     3241 ctttgagtta tcgccaccag gtcgccgaag agcttggcaa gctgggctct attcaggata
     3301 aatcccgcgg atggtgggtt cactccgttc tcttgctcga ggccaccaca ttccgcaccg
     3361 taggattact gcatcaggag tggtggatgc gcccggatga ccctgccgat gcggatgaaa
     3421 aggagagtgg caaatggctg gcagcggccg caactagccg gttacgcatg ggcagcatga
     3481 tgagcaacgt gattgcggtc tgtgaccgcg aagccgatat tcatgcttat ctgcaggaca
     3541 aactggcgca taacgagcgc ttcgtggtgc gctccaagca cccacgcaag gacgtagagt
     3601 ctgggttgta tctgtacgac catctgaaga accaaccgga gttgggtggc tatcagatca
     3661 gcattccgca aaagggcgtg gtggataaac gcggtaaacg taaaaatcga ccagcccgca
     3721 aggcgagctt gagcctgcgc agtgggcgca tcacgctaaa acaggggaat atcacgctca
     3781 acgcggtgct ggccgaggag attaacccgc ccaagggtga gaccccgttg aaatggttgt
     3841 tgctgaccag cgaaccggtc gagtcgctag cccaagcctt gcgcgtcatc gacatttata
     3901 cccatcgctg gcggatcgag gagttccata aggcatggaa aaccggagca ggagccgaga
     3961 ggcaacgcat ggaggagccg gataatctgg agcggatggt ctcgatcctc tcgtttgttg
     4021 cggtcaggct gttacagctc agagaaagct tcacgctgcc gcaagcactc agggcgcaag
     4081 ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
     4141 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
     4201 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
     4261 gaaccggaat tgccagctgg ggcgccctct gggaaggttg ggaagccctg caaagtaaac
     4321 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgacccggga
     4381 ctagttggtg tggaagtcca tatatcggtg atcaatatag tggttgacat gctggctagt
     4441 caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca aatatcaaac
     4501 ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa gaaaaaaatt
     4561 actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg tctcaccata
     4621 gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata catataatac
     4681 acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa attggctagt
     4741 gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg taaaagttca
     4801 ctttagcata catatattac acgagccaga aattgtaact tttgcctaaa atcacaaatt
     4861 gcaaaattta attgcttgca aaaggtcaca tgcttataat caactttttt aaaaatttaa
     4921 aatacttttt tattttttat ttttaaacat aaatgaaata atttatttat tgtttatgat
     4981 taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt tgtccttgga aaaaaagcac
     5041 tacctaggag cggccaaaat gccgaggctt tcatagctta aactctttac agaaaatagg
     5101 cattatagat cagttcgagt tttcttattc ttccttccgg ttttatcgtc acagttttac
     5161 agtaaataag tatcacctct tagagttaac tatgagataa gcaagtatca tctcatttca
     5221 tttacctgaa gtcgagtaaa cagaaaatcc aattgttgat gaacctcaat gacttagaac
     5281 tatctatcgg cagatcatat aaagaggatt taggtactcg agaacgctcc gtacatgcag
     5341 ctaaacctcc aagcatctct aacttgggta gtgctttctc caccattttc tgtgcttgct
     5401 ccttcgtggc aagtccagcc cataatgccc agaatgtagt tgcggattcg tatgacgttc
     5461 tgtgcttgat ttttgtgttg tagtcaaaga aaaaccccga ctcgtcatcc cacatatatt
     5521 tggtaattga tgaggcaacg ctaattatca acatatagat tgttatctat ctgcatgaac
     5581 acgaaatctt tacttgacga cttgaggctg atggtgttta tgcaaagaaa ccactgtgtt
     5641 taatatgtgt cactgtttga tattactgtc agcgtagaag ataatagtaa aagcggttaa
     5701 taagtgtatt tgagataagt gtgataaagt ttttacagcg aaaagacgat aaatacaaga
     5761 aaatgattac gaggatacgg agagaggtat gtacatgtgt atttatatac taagctgccg
     5821 gcggttgttt gcaagaccga gaaaaggcta gcaagaatcg ggtcattgta gcgtatgcgc
     5881 ctgtgaacat tctcttcaac aagtttgatt ccattgcggt gaaatggtaa aagtcaaccc
     5941 cctgcgatgt atattttcct gtacaatcaa tcaaaaagcc aaatgattta gcattatctt
     6001 tacatcttgt tattttacag attttatgtt tagatctttt atgcttgctt ttcaaaaggc
     6061 ctgcaggcaa gtgcacaaac aatacttaaa taaatactac tcagtaataa cctatttctt
     6121 agcatttttg acgaaatttg ctattttgtt agagtctttt acaccatttg tctccacacc
     6181 tccgcttaca tcaacaccaa taacgccatt taatctaagc gcatcaccaa cattttctgg
     6241 cgtcagtcca ccagctaaca taaaatgtaa gctctgcctc gcgcgtttcg gtgatgacgg
     6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7381 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7441 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagg
     7501 agctcgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
     7561 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
     7621 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
     7681 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
     7741 gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg
     7801 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
     7861 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     7921 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     7981 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     8041 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     8101 atagccgcgc tgcctcgtct tggagttcat tcagggcacc ggacaggtcg gtcttgacaa
     8161 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     8221 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     8281 gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg
     8341 atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct
     8401 tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa
     8461 ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc
     8521 ttgcgttttc ccttgtccag atagcccagt agctgacatt catccgggcg atcgatggaa
     8581 gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc
     8641 ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct
     8701 ccagcagccg cacgcggcgc atcggggggg gggtttcaat tcaattcatc attttttttt
     8761 tattcttttt tttgatttcg gtttctttga aatttttttg attcggtaat ctccgaacag
     8821 aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
     8881 gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg
     8941 aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
     9001 cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
     9061 ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
     9121 tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
     9181 ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct
     9241 gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
     9301 gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag
     9361 gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc
     9421 aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac
     9481 aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac
     9541 gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa
     9601 cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga
     9661 ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc
     9721 tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat
     9781 gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attacccggg
     9841 aatctcggtc gtaatgattt ttataatgac gaaaaaaaaa aaattggaaa gaaaaccccc
     9901 cccccgcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac
     9961 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
    10021 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
    10081 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt
    10141 ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac
    10201 gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag
    10261 ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
    10321 gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatt cccccttaca
    10381 cggaggcatc aagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga
    10441 agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac
    10501 atctgtgaat cgcttcacga ccacgctgat gagctttacc gcaggtgg
//
