LOCUS       Exported                9803 bp ds-DNA     circular SYN 21-JAN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 21, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9803
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    533..1240
                     /label=mCherry
                     /note="color: #c0000c; direction: RIGHT"
     misc_feature    1283..1804
                     /label=SYNAPTOZIP
                     /note="color: #84a4c0; direction: RIGHT"
     misc_feature    1682..1771
                     /label=ZIP
                     /note="color: #ff00ff; direction: RIGHT"
     CDS             1772..1801
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /note="color: #cc99b2"
                     /translation="EQKLISEEDL"
     misc_feature    1805..3230
                     /label=3'UTR VAMP2
                     /note="color: #339966"
     misc_feature    3244..3832
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(3715..3726)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3714"
                     /translation="IEGR"
     LTR             3919..4152
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    4224..4345
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      4364..4499
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(4532..4550)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(4560..4576)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      4721..5176
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5205..5309
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5310..6170
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5310 .. 5378 / #ccffcc / signal sequence
                        2: 5379 .. 6170 / #ccffcc
                     Cleavage site after base 5378"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6341..6929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    7217..7238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        7253..7283
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 7253 .. 7258 / #ffffff / -35
                        2: 7259 .. 7276 / #ffffff
                        3: 7277 .. 7283 / #ffffff / -10"
     protein_bind    7291..7307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     7315..7331
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        7352..7370
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     enhancer        7471..7850
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        7851..8054
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             8069..8249
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    8293..8418
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    8911..9144
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    9640..9757
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcggggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtcgcca ccatggtgag
      541 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
      601 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      661 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
      721 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
      781 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
      841 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
      901 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
      961 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     1021 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     1081 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     1141 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     1201 gggccgccac tccaccggag gcatggacga gctgtacaag ggcaccggcg cgcctcagat
     1261 ctcgagctca agcttcgaat tctcggctac cgctgccacc gtcccgcctg ccgccccggc
     1321 cggcgagggt ggcccccctg cacctcctcc aaatcttacc agtaacagga gactgcagca
     1381 gacccaggcc caggtggatg aggtggtgga catcatgagg gtgaatgtgg acaaggtcct
     1441 ggagcgagac cagaagctat cggaactgga tgatcgcgca gatgccctcc aggcaggggc
     1501 ctcccagttt gaaacaagtg cagccaagct caagcgcaaa tactggtgga aaaacctcaa
     1561 gatgatgatc atcttgggag tgatttgcgc catcatcctc atcatcatca tcgtttactt
     1621 cagcacttct gtccctgagg gtaagggcgt ggagccaaag acctactgct actacagcag
     1681 cgctcagctg gagaaggagc tgcaggccct ggagaaggag aacgcacaac tagaatggga
     1741 acttcaagcg ctcgaaaaag aactggctca ggaacaaaaa ctgatcagcg aggaggacct
     1801 gtgacctgag gttcccatca cctctctctc tcaccctctg ccccctttcc tctttcctgt
     1861 tcagccccaa gtcccttcat ttgcatctgc tatgcaatag cccctctcct ccttcccttg
     1921 gatttaacca atccttcccc tatcttccct gtacaattcc agaccctccc caaacagagc
     1981 aaaccccaca gaaacaaaca aaacacccca cctgtctagg cttccatagg ttgcatcttt
     2041 gtatcccttg ggagcctcta agactggtcc tacttggtcc taagaatccc aaggttttct
     2101 gggagcttcc aacatgttga ttagcatatc atttgcatac actgtctttt ttttttccct
     2161 gggtttcttc ctttctgttc cattactctt cactcttctg tgtttttttt ttttctttct
     2221 ttcttttcgg aagagttagt ttccattggt cctcttatca cacttttatt tgcacgacat
     2281 tatctgcagg tggtggggag cctgggcttt tggggaacta ggaccccaaa gttgcctcct
     2341 cccagcccct ctagtctagt ttgcttccct taccccattt tccacacctc ttgtaccctc
     2401 ctctccctcc cccagctggt gtgtaagtgt cttggagttc agtgtgttat gatggaccaa
     2461 taattctgcc acttggggtc tctccctaca ttcctgctcc ccagttttcg tgtggggcat
     2521 tcagctgaca ttcccggggg tctccctccc tcccatctgc ctgatctgct gcctcctccc
     2581 accaggagaa ctggaggctg accacaatct ggttctttga ggaggggtgg ctctagtgtg
     2641 tgtgggggtc atcactgcct tggggaggag tgggacaaaa cagaaccccc cctaattcct
     2701 gcctgaaatc tctggcctca tccttgctag aggttggact gaaaactttc ctccccaatc
     2761 ctgggggggg gtattacccc ccatcactgc ccagctcctc tgactgcccc cctgtattca
     2821 gggtgggggt actagtcact gccaatatgt gtatgggact tgctggagga tggggatcct
     2881 tgtccttctc tagggctgct gagccctgag agagagagag agagagagag agagagagag
     2941 agagagagag agagagagag ggaagcaagc tatttccctg ttgggtacaa tgatagagaa
     3001 aggatctaat ctttaaaaat ggcacagttt taggggtctg agggtacagc cccttaacct
     3061 gcctcttggg gggggtgttc cccaaactct tcccccacac acaccaggtt ttctgtgtgg
     3121 aggggaacca aggagatcta aactgtggtg tgaaagggta ggagagatgc tgggggtggg
     3181 ggtgcttgtg ttttagaccc ccaatattat cccagtgtcc cctgcctgaa ttctgcagtc
     3241 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3301 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3361 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3421 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3481 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3541 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3601 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     3661 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3721 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3781 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     3841 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3901 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3961 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     4021 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4081 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4141 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     4201 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     4261 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4321 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     4381 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4441 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4501 aggcttttgc gtcgagacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     4561 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4621 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4681 ccttcccaac agttgcgcag cctgaatggc gaatggcgcg acgcgccctg tagcggcgca
     4741 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4801 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4861 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4921 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4981 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5041 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5101 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5161 ttaacgttta caatttccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5221 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5281 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5341 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5401 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5461 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5521 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5581 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5641 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5701 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5761 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5821 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5881 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5941 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6001 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6061 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6121 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6181 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6241 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6301 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6361 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6421 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6481 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6541 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6601 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6661 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6721 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6781 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6841 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6901 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6961 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7021 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7081 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7141 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7201 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7261 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7321 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     7381 tggagctgca agcttggcca ttgcatacgt tgtatccata tcataatatg tacatttata
     7441 ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt tattaatagt
     7501 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     7561 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     7621 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     7681 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     7741 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     7801 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     7861 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     7921 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     7981 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     8041 atataagcag agctcgttta gtgaaccggg gtctctctgg ttagaccaga tctgagcctg
     8101 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8161 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8221 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggacct gaaagcgaaa
     8281 gggaaaccag agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     8341 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     8401 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     8461 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     8521 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     8581 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     8641 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     8701 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     8761 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     8821 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     8881 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     8941 agcaggaagc actatgggcg cagcctcaat gacgctgacg gtacaggcca gacaattatt
     9001 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     9061 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     9121 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     9181 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     9241 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     9301 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     9361 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     9421 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     9481 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     9541 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     9601 cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat
     9661 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     9721 agaattacaa aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga
     9781 gaagcttgat atcgaattcc cac
//
