LOCUS       pLenti-TO/CMV-pu        9679 bp    DNA     circular SYN  7-DEC-2010
DEFINITION  pLenti CMV/TO Puro DEST (670-1) Sequence.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|572960330|
COMMENT     VNTDBDATE|572960456|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLenti-TO/CMV-puro-Fv1E-RKD|
COMMENT     VNTAUTHORNAME|Xiaolin Nan|
COMMENT     VNTAUTHOREML|nan@ohsu.edu|
COMMENT     VNTAUTHORAD1|3303 SW Bond Ave|
COMMENT     VNTAUTHORAD2|Portland, OR 97239|
COMMENT     VNTREPLTYPE|Plasmid
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COMMENT                          "pLenti CMV/TO Puro DEST (670-1) Sequence" 1) 
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COMMENT                          "Foreign object. Author: Xiaolin Nan. Original author: Xiaolin Nan" 
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COMMENT                          "Created: 12/07/10 11:38AM" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="pLenti CMV/TO Puro DEST (670-1) Sequence"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        62..90
                     /vntifkey="30"
                     /label=AmpR_promoter
     CDS             132..992
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 3"
     gene            132..992
                     /gene="Ampicillin"
                     /vntifkey="60"
     rep_origin      1147..1766
                     /vntifkey="33"
                     /label=pBR322_origin
     promoter        2075..2104
                     /vntifkey="30"
                     /label=lac_promoter
     misc_feature    2118..2140
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     misc_feature    2139..2157
                     /vntifkey="21"
                     /label=M13_reverse_primer
     promoter        2174..2193
                     /vntifkey="30"
                     /label=T3_promoter
     misc_feature    2448..2628
                     /vntifkey="21"
                     /label=HIV-1_5_LTR
     misc_feature    2448..2628
                     /vntifkey="21"
                     /label=truncHIV-1_3_LTR
     misc_feature    2736..2780
                     /vntifkey="21"
                     /label=HIV-1_psi_pack
     misc_feature    3290..3523
                     /vntifkey="21"
                     /label=RRE
     misc_feature    4016..4031
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    4156..4443
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        4149..4653
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    4610..4630
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     misc_feature    4661..4700
                     /vntifkey="21"
                     /label=tetO
     misc_feature    6563..7138
                     /vntifkey="21"
                     /label=WPRE
     CDS             6651..7280
                     /vntifkey="4"
                     /label=ORF\frame\2
     misc_feature    complement(7198..7221)
                     /vntifkey="21"
                     /label=MSCV_rev_primer
     misc_feature    7595..7614
                     /vntifkey="21"
                     /label=mPGK_F_primer
     CDS             7443..8282
                     /gene="puro(variant)"
                     /vntifkey="4"
                     /label=puro(variant)
                     /note="ORF frame 2"
     CDS             complement(7608..8333)
                     /vntifkey="4"
                     /label=ORF\frame\2
     gene            7683..8282
                     /gene="puro(variant)"
                     /vntifkey="60"
     misc_feature    8403..8418
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    8403..8424
                     /vntifkey="21"
                     /label=U3PPT
     misc_feature    8473..8653
                     /vntifkey="21"
                     /label=HIV-1_5_LTR
     misc_feature    8473..8653
                     /vntifkey="21"
                     /label=truncHIV-1_3_LTR
     terminator      8728..8847
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    8816..8835
                     /vntifkey="21"
                     /label=EBV_rev_primer
     rep_origin      8865..8942
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    8927..8946
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     promoter        complement(9033..9051)
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(9061..9077)
                     /vntifkey="21"
                     /label=M13_forward20_primer
     misc_feature    complement(9070..9092)
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     misc_feature    9070..9213
                     /vntifkey="21"
                     /label=lacZ_a
     rep_origin      9239..9545
                     /vntifkey="33"
                     /label=f1_origin
     CDS             4786..5151
                     /vntifkey="4"
                     /label=Fv1E
     CDS             5182..5889
                     /vntifkey="4"
                     /label=PAmCherry1
     CDS             5914..6480
                     /vntifkey="4"
                     /label=KRas\4B\G12D
BASE COUNT     2430 a      2465 c      2556 g      2228 t 
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 
     4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 
     4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct 
     4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
     4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
     4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 
     4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
     4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
     4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
     4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
     4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
     4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat 
     4681 ctccctatca gtgatagaga tcgtcgacta gtccagtgtg gtggaattct gcagatatca 
     4741 acaagtttgt acaaaaaagc aggctccgcg gccgccccct tcaccatggc ttctagagga 
     4801 gtgcaggtgg agactatctc cccaggagac gggcgcacct tccccaagcg cggccagacc 
     4861 tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga aagttgattc ctcccgggac 
     4921 agaaacaagc cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa 
     4981 ggggttgccc agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc 
     5041 tatggtgcca ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg 
     5101 gagcttctaa aactggaaac tagttatccg tacgacgtac cagactacgc aaagcttctc 
     5161 gagctgcagg agctcggtac catggtgagc aagggcgagg aggataacat ggccatcatc 
     5221 aaggagttca tgcgcttcaa ggtgcatatg gagggctccg tgaacggcca cgtgttcgag 
     5281 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 
     5341 accaagggtg gccccctgcc cttcacctgg gacatcctgt cccctcagtt catgtacggc 
     5401 tccaacgcct acgtgaagca ccccgccgac atccccgact acttcaagct gtccttcccc 
     5461 gagggcttca agtgggagcg cgtgatgaag ttcgaggacg gcggcgtggt gaccgtgacc 
     5521 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 
     5581 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc cctctccgag 
     5641 cggatgtacc ccgaggacgg cgccctgaag ggcgaggtca agccaagggt caagctgaag 
     5701 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag 
     5761 ctgcccggcg cctacaacgt caaccgcaag ttggacatca cctcccacaa cgaggactac 
     5821 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag 
     5881 ctgtacaaga gatctcatat gccatgggtc gacatgactg aatataaact tgtggtagtt 
     5941 ggagctgatg gcgtaggcaa gagtgccttg acgatacagc taattcagaa tcattttgtg 
     6001 gacgaatatg atccaacaat agaggattcc tacaggaagc aagtagtaat tgatggagaa 
     6061 acctgtctct tggatattct cgacacagca ggtcaagagg agtacagtgc aatgagggac 
     6121 cagtacatga ggactgggga gggctttctt tgtgtatttg ccataaataa tactaaatca 
     6181 tttgaagata ttcaccatta tagagaacaa attaaaagag ttaaggactc tgaagatgta 
     6241 cctatggtcc tagtaggaaa taaatgtgat ttgccttcta gaacagtaga cacaaaacag 
     6301 gctcaggact tagcaagaag ttatggaatt ccttttattg aaacatcagc aaagacaaga 
     6361 cagggtgttg atgatgcctt ctatacatta gttcgagaaa ttcgaaaaca taaagaaaag 
     6421 atgagcaaag atggtaaaaa gaagaaaaag aagtcaaaga caaagtgtgt aattatgtaa 
     6481 ggatcctaaa agggtgggcg cgccgaccca gctttcttgt acaaagtggt tgatatccag 
     6541 cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg 
     6601 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 
     6661 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 
     6721 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 
     6781 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 
     6841 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 
     6901 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga 
     6961 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 
     7021 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 
     7081 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 
     7141 cctccccgcc tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca 
     7201 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca 
     7261 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca 
     7321 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg 
     7381 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag 
     7441 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc 
     7501 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc 
     7561 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg 
     7621 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta 
     7681 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg 
     7741 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg 
     7801 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 
     7861 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 
     7921 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 
     7981 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 
     8041 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 
     8101 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 
     8161 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 
     8221 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct 
     8281 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc 
     8341 gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 
     8401 ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt 
     8461 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctgc 
     8521 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 
     8581 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 
     8641 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 
     8701 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 
     8761 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
     8821 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 
     8881 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     8941 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     9001 taggcttttg cgtcgagacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc 
     9061 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 
     9121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 
     9181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc 
     9241 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 
     9301 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 
     9361 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 
     9421 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 
     9481 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 
     9541 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 
     9601 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 
     9661 attaacgttt acaatttcc 
//
