LOCUS       pBT009.J1.117.J306      3124 bp ds-DNA     circular     20-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(1..683)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(781..1440)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1987..2006
                     /label="Seq6-s"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2138..2144
                     /label="Ptar promoter"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2181..2350
                     /label="J1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2351..2385
                     /label="J23117"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2386..2405
                     /label="J306"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2387..2405
                     /label="J306-19"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2406..2447
                     /label="JAD designed 3' chimera "
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2453..2466
                     /label="Hairpin3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2467..2489
                     /label="MS2 +2Bp extension"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2490..2496
                     /label="SUP4"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      2497..2549
                     /label="ECK120033736 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2965..2982)
                     /label="Seq7-as"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(3017..3036)
                     /label="Seq8-as"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
       61 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
      121 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
      181 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC
      241 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
      301 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
      361 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
      421 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC
      481 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
      541 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
      601 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
      661 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
      721 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
      781 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
      841 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
      901 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
      961 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
     1021 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
     1081 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG
     1141 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
     1201 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
     1261 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
     1321 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
     1381 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT
     1441 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
     1501 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
     1561 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
     1621 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
     1681 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
     1741 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT
     1801 AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTT CAAGAATTCT GGCGAATCCT
     1861 CTGACCAGCC AGAAAACGAC CTTTCTGTGG TGAAACCGGA TGCTGCAATT CAGAGCGGCA
     1921 GCAAGTGGGG GACAGCAGAA GACCTGACCG CCGCAGAGTG GATGTTTGAC ATGGTGAAGA
     1981 CTATCGCACC ATCAGCCAGA AAACCGAATT TTGCTGGGTG GGCTAACGAT ATCCGCCTGA
     2041 TGCGTGAACG TGACGGACGT AACCACCGCG ACATGTGTGT GCTGTTCCGC TGGGCATGCC
     2101 AGGACAACTT CTGGTCCGGT AACGTGCTGA GCCCGGCCAA GCTTAGCccg aaaagtgcca
     2161 cctgTGGCAA TTCCGACGTC GCCTACGGTA TCCACCGGAG ACCTATGGCA GCCTCCGGCC
     2221 GCCATAGGAC ACCTTTGGTT GCCAAGGGTG ACCTATGGTG ACCATGGGCC ACCACGGGCG
     2281 ACCTCAGGTA TCCTGCGGTG TCCTGCGGTT ACCAAAGGCG TCCTTTGGGT TCCACCGGAT
     2341 ACCTCCGGAC ttgacagcta gctcagtcct agggattgtg ctagcTTGTG TCCAGAACGC
     2401 TCCGTGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA
     2461 AAAGTGGCAC ATGAGGATCA CCCATGTGCT TTTTTTaacg catgagAAAG CCCCCGGAAG
     2521 ATCACCTTCC GGGGGCTTTt ttattgcgcG GATCCTTACT CCGTCGACAA GCTTGCGGCC
     2581 GCACTCGAGG AATTCGACTC AATTAGTTCA GTCAGTTTCA GGATATTAGT CATCTCTACA
     2641 TTGATTATGA GTATTCAGAA ATTCCTTAAA TATTCTGACA AATGCTCTTT CCCTAAACTC
     2701 CCCCCATAAA AAAACCCGCC GAAGCGGGTT TTTACGTTAT TTGCGGATTA ACGATTACTC
     2761 GTTATCAGAA CCGCCCAGAC CTGCGTTCAG CAGTTCTGCC AGGCTGGCAG ATGCGTCTTC
     2821 CGAATTGATC CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG
     2881 GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT
     2941 AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG
     3001 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT
     3061 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA
     3121 AAAA
//