LOCUS       pBT009.J3_117_RR2       3115 bp ds-DNA     circular     20-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(451..1110)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1657..1676
                     /label="Seq6-s"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1808..1814
                     /label="Ptar promoter"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1851..2020
                     /label="J3"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2021..2055
                     /label="J23117"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2056..2075
                     /label="RR2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2056..2075
                     /label="RR2gRNA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2056..2075)
                     /label="RR2"
                     /ApEinfo_revcolor=#e0413b
                     /ApEinfo_fwdcolor=#e0413b
     misc_feature    2076..2117
                     /label="JAD designed chimera RNA 3'UTR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2122..2157
                     /label="Streptococcus pyogenes tracrRNA terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      2158..2210
                     /label="ECK120033736 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2626..2643)
                     /label="Seq7-as"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2678..2697)
                     /label="Seq8-as"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(join(2786..3115,1..353))
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
       61 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
      121 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
      181 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
      241 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC
      301 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
      361 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
      421 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
      481 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
      541 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC
      601 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
      661 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
      721 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
      781 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
      841 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
      901 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
      961 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     1021 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
     1081 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     1141 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     1201 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     1261 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     1321 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     1381 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA
     1441 CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC
     1501 CTTTCGTCTT CAAGAATTCT GGCGAATCCT CTGACCAGCC AGAAAACGAC CTTTCTGTGG
     1561 TGAAACCGGA TGCTGCAATT CAGAGCGGCA GCAAGTGGGG GACAGCAGAA GACCTGACCG
     1621 CCGCAGAGTG GATGTTTGAC ATGGTGAAGA CTATCGCACC ATCAGCCAGA AAACCGAATT
     1681 TTGCTGGGTG GGCTAACGAT ATCCGCCTGA TGCGTGAACG TGACGGACGT AACCACCGCG
     1741 ACATGTGTGT GCTGTTCCGC TGGGCATGCC AGGACAACTT CTGGTCCGGT AACGTGCTGA
     1801 GCCCGGCCAA GCTTAGCccg aaaagtgcca cctgTGGCAA TTCCGACGTC AGCATTTGCG
     1861 ATCATTCACG CAGCGCTTAT TCAGTTGCTC ACTGCGATGT CATAATCATC GCTACGAGCT
     1921 GTGAAAGATG CATAAAGCTC GTACGACGCG TTCGCTCGTC TCCTCACTTC TCCTACGGAG
     1981 CGTTCTGGAC ACAACGTCGT CTTGAAGTTG CGATTATAGA ttgacagcta gctcagtcct
     2041 agggattgtg ctagcTGGAA CCGTACTGGA ACTGCGTTTT AGAGCTAGAA ATAGCAAGTT
     2101 AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTaac
     2161 gcatgagAAA GCCCCCGGAA GATCACCTTC CGGGGGCTTT tttattgcgc GGATCCTTAC
     2221 TCCGTCGACA AGCTTGCGGC CGCACTCGAG GAATTCGACT CAATTAGTTC AGTCAGTTTC
     2281 AGGATATTAG TCATCTCTAC ATTGATTATG AGTATTCAGA AATTCCTTAA ATATTCTGAC
     2341 AAATGCTCTT TCCCTAAACT CCCCCCATAA AAAAACCCGC CGAAGCGGGT TTTTACGTTA
     2401 TTTGCGGATT AACGATTACT CGTTATCAGA ACCGCCCAGA CCTGCGTTCA GCAGTTCTGC
     2461 CAGGCTGGCA GATGCGTCTT CCGAATTGAT CCGTCGACCG ATGCCCTTGA GAGCCTTCAA
     2521 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT
     2581 CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA
     2641 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
     2701 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
     2761 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
     2821 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
     2881 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
     2941 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT
     3001 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
     3061 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGA
//