LOCUS       pF1_CDK1/cyclin_B1      7524 bp ds-DNA     circular     30-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             885..1544
                     /label="AmpR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      1642..2324
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2611..2835
                     /label="TN7R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2902..3435
                     /label="Genta"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4169..4217
                     /label="HSV TK polyA signal"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4362..4364
                     /label="STOP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    4362..4451
                     /label="Strep-tag II"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(4362..4484)
                     /label="Translation 4484-4362"
     misc_feature    complement(4452..4469)
                     /label="TEV cut"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(4485..5783)
                     /label="Cyclin B1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(4485..5783)
                     /label="Translation 5783-4485"
     misc_feature    complement(5784..5894)
                     /label="p10 promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    5938..6027
                     /label="polh promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6030..6920
                     /label="CDK1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             6030..6920
                     /label="Translation 6030-6920"
     misc_feature    6921..6935
                     /label="end"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             6921..6983
                     /label="Translation 6921-6983"
     misc_feature    6936..6959
                     /label="Precission site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    6960..6983
                     /label="8HISTAG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     polyA_signal    complement(7053..7244)
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    7277..7442
                     /label="TN7L"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 TTCTCTGTCA CAGAATGAAA ATTTTTCTGT CATCTCTTCG TTATTAATGT TTGTAATTGA
       61 CTGAATATCA ACGCTTATTT GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC
      121 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT
      181 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG
      241 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA
      301 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT
      361 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTAATAGTG GACTCTTGTT CCAAACTGGA
      421 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG
      481 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA
      541 TTAACGCTTA CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT
      601 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC
      661 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC
      721 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA
      781 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG
      841 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG
      901 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC
      961 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA
     1021 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG
     1081 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA
     1141 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC
     1201 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT
     1261 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG
     1321 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA
     1381 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA
     1441 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA
     1501 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG
     1561 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG
     1621 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT
     1681 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG
     1741 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC
     1801 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA
     1861 CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA
     1921 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC
     1981 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG
     2041 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC
     2101 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG
     2161 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT
     2221 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT
     2281 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
     2341 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
     2401 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
     2461 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC
     2521 TGTGCGGTAT TTCACACCGC ATAGACCAGC CGCGTAACCT GGCAAAATCG GTTACGGTTG
     2581 AGTAATAAAT GGATGCCCTG CGTAAGCGGG TGTGGGCGGA CAATAAAGTC TTAAACTGAA
     2641 CAAAATAGAT CTAAACTATG ACAATAAAGT CTTAAACTAG ACAGAATAGT TGTAAACTGA
     2701 AATCAGTCCA GTTATGCTGT GAAAAAGCAT ACTGGACTTT TGTTATGGCT AAAGCAAACT
     2761 CTTCATTTTC TGAAGTGCAA ATTGCCCGTC GTATTAAAGA GGGGCGTGGC CAAGGGCATG
     2821 GTAAAGACTA TATTCGCGGC GTTGTGACAA TTTACCGAAC AACTCCGCGG CCGGGAAGCC
     2881 GATCTCGGCT TGAACGAATT GTTAGGTGGC GGTACTTGGG TCGATATCAA AGTGCATCAC
     2941 TTCTTCCCGT ATGCCCAACT TTGTATAGAG AGCCACTGCG GGATCGTCAC CGTAATCTGC
     3001 TTGCACGTAG ATCACATAAG CACCAAGCGC GTTGGCCTCA TGCTTGAGGA GATTGATGAG
     3061 CGCGGTGGCA ATGCCCTGCC TCCGGTGCTC GCCGGAGACT GCGAGATCAT AGATATAGAT
     3121 CTCACTACGC GGCTGCTCAA ACTTGGGCAG AACGTAAGCC GCGAGAGCGC CAACAACCGC
     3181 TTCTTGGTCG AAGGCAGCAA GCGCGATGAA TGTCTTACTA CGGAGCAAGT TCCCGAGGTA
     3241 ATCGGAGTCC GGCTGATGTT GGGAGTAGGT GGCTACGTCT CCGAACTCAC GACCGAAAAG
     3301 ATCAAGAGCA GCCCGCATGG ATTTGACTTG GTCAGGGCCG AGCCTACATG TGCGAATGAT
     3361 GCCCATACTT GAGCCACCTA ACTTTGTTTT AGGGCGACTG CCCTGCTGCG TAACATCGTT
     3421 GCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCAAACA TCGACCCACG GCGTAACGCG
     3481 CTTGCTGCTT GGATGCCCGA GGCATAGACT GTACAAAAAA ACAGTCATAA CAAGCCATGA
     3541 AAACCGCCAC TGCGCCGTTA CCACCGCTGC GTTCGGTCAA GGTTCTGGAC CAGTTGCGTG
     3601 AGCGCATACG CTACTTGCAT TACAGTTTAC GAACCGAACA GGCTTATGTC AACTGGGTTC
     3661 GTGCCTTCAT CCGTTTCCAC GGTGTGCGTC ACCCGGCAAC CTTGGGCAGC AGCGAAGTCG
     3721 AGGCATTTCT GTCCTGGCTG GCGAACGAGC GCAAGGTTTC GGTCTCCACG CATCGTCAGG
     3781 CATTGGCGGC CTTGCTGTTC TTCTACGGCA AGGTGCTGTG CACGGATCTG CCCTTGCTTC
     3841 AGGAGATCGG TAGACCTCGG CCGTCGCGGC GCTTGCCGGT GGTGCTGACC CCGGATGAAG
     3901 TGGTTCGCAT CCTCGGTTTT CTGGAAGGCG AGCATCGTTT GTTCGCCCAG GACTCTAGCT
     3961 ATAGTTCTAG TGGTTGGCTA CAGCTTTGTT TAAACAAAGC TGGCTATGGC AGGGCTTGCC
     4021 GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGTTG GGGTGGGGAA
     4081 AAGGAAGAAA CGCGGGCGTA TTGGTCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC
     4141 CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCCGT GCGTTTTATT
     4201 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC
     4261 AGTTAGCCTC CCCCATCTCC CGGTACCGCA TGCTTTATTT TTCAAATTGC GGATGGCTCC
     4321 ATGGAATGCT GGATTTGGTA CCGCATGCTA TGCATCAGCT GTTACTTCTC GAACTGAGGG
     4381 TGAGACCAAG AACCTCCGCC GCTACCTCCG CCGGATCCGC CACCTTTTTC AAATTGCGGA
     4441 TGGCTCCATG ACTGGAAGTA CAGGTTTTcc tcagctaGCG ATGACACCTT AGCCACAGCC
     4501 TTAGCCAAGT CCTGCACCAG AGCAGAGTTC AGCTGGGGCA GAGTGGAGAT CTTGGCGTGC
     4561 TTGGAGGTGG CGTATTTGTT CTTCACAGTC ATGTGCTTGG TCAGGCCCTG GTTGACCATG
     4621 ACCACGTTCT TGGCCAGGTG CTGCATCACA GGCAGCAGGG ATTCCTCGGT GTAGGACAGG
     4681 TAGTGCTGCA GAGTAGGGGT CCACTCGCCG TTGTCCAGGA TCTTGAGAGC CAGGCAGAAA
     4741 GCACCAGCAG CGATCTGAGA AGGTGGGAAG TGCACCATGT CGTAGTCCAG CATGGTCAGT
     4801 TCCATCAGGT ACTTAGCCAG GGTGTGCTGT TCCACGTCCA CCTCTCCGAT CTTGGAAGCA
     4861 CGACGCAGGA AGTGCAGAGG CAAAGGTCTA CCCAGGCCGA AGTTCAGAGC GCGCAGGATC
     4921 TTCATTTCCA TCTGACGGAT CTGGTGCTTG GTGTAGGTGT TGTCGGTCAC GAAAGCGAAG
     4981 TCGCCGATCT CAGGAGGGTA CATTTCCTCG TACTTAGAGG CAATGAACAT AGCGGTGACA
     5041 CCCACGAGCT GCAGCATTTT CTTGGGCACG CAGTTGTTCT GCATGAAGCG GTCGATGATG
     5101 GACACGGTCA TGTACATAGT CTCTTGCAGC AGACGGAACT TCATCTGCAC CTGGACCAGC
     5161 CAGTCGATCA GGATAGCACG CATGTTGCCG GTCACTTCAC GACCCAGCAG GTACTTGGGA
     5221 CGCACAGCCT GTTCCTCTTC CAGTTGACGC AGGTAAGCGT AGATGTCCTT CACGTACTCG
     5281 GAGCACAGGT TAGGGTCAGC ACCGTCCTCA GCGTCCACGT CGTTAGCAGC CAGGATCACG
     5341 TCGGAGAAAG CCTGGCACAG GTCCTCTTCA GCTGGAGCGC AACCGGAGGT TTCCATAGGT
     5401 GAGGGTGAAG CGGTGTCCAC CAGGATAGGC TCAGGGGACA GCTTTTCCTC TTTCACAGGA
     5461 CCAGGTTCTG GCTCTGGTTC AGGCTCAGGC ACAGGTTCAG ACACAGGCAC GGGCACCAAC
     5521 ATGGGCACCT TCTCGAGAGG CTTGGGCAGC TTCTTGTCGA TCACTTTGCC GGTAGCGGAG
     5581 GGCTTAGCCT CTTTCTTCAT GGGCATCTTA GCCTGCAGCT GCTCGGACAC CTTGTTTCCG
     5641 ATGTCTCCCA GAGCGGTACG AGGACGCAGT CCAGGCTTAG AAGTAGCAGC AGGAGCGGTG
     5701 GGCACACGCT TAGCACCAGC CATGTTGATC TTCGCCTTGT TCTCAGCGTT GATCTTGGAG
     5761 TTACGGGTCA CACGCAGAGC CATGATTGTA AATAAAATGT AATTTACAGT ATAGTATTTT
     5821 AATTAATATA CAAATGATTT GATAATAATT CTTATTTAAC TATAATATAT TGTGTTGGGT
     5881 TGAATTAAAG GTCCGTATAC TAGTATCGAT TCGCGACCTA CTCCGGAATA TTAATAGATC
     5941 ATGGAGATAA TTAAAATGAT AACCATCTCG CAAATAAATA AGTATTTTAC TGTTTTCGTA
     6001 ACAGTTTTGT AATAAAAAAA CCTATAAATA TGGAAGATTA CACCAAGATC GAGAAGATCG
     6061 GCGAGGGCAC CTACGGTGTC GTGTACAAGG GTCGTCACAA GACCACCGGA CAGGTGGTGG
     6121 CTATGAAGAA GATCCGTCTG GAATCCGAGG AAGAGGGCGT CCCCTCTACC GCTATCCGTG
     6181 AAATCTCCCT GCTGAAAGAG CTGCGTCACC CCAACATCGT GTCCCTGCAA GACGTGCTGA
     6241 TGCAGGACTC CCGTCTGTAC CTGATCTTCG AGTTCCTGTC TATGGACCTG AAGAAGTACC
     6301 TGGACTCTAT CCCTCCTGGC CAGTACATGG ACTCCAGCCT GGTCAAGTCC TACCTGTACC
     6361 AGATCCTGCA GGGTATCGTG TTCTGCCACT CTCGTCGTGT GCTGCACCGT GACCTGAAGC
     6421 CTCAGAACCT GCTGATCGAC GACAAGGGCA CCATCAAGCT GGCTGACTTC GGACTGGCTC
     6481 GTGCTTTCGG TATCCCCATC CGTGTGTACA CCCACGAGGT GGTCACTCTG TGGTATCGTT
     6541 CTCCCGAGGT GCTGCTGGGT TCCGCTCGTT ACTCTACCCC TGTGGACATC TGGTCCATCG
     6601 GCACCATCTT CGCTGAGCTG GCTACCAAGA AACCCCTGTT CCACGGCGAC TCCGAGATCG
     6661 ACCAACTGTT CCGTATCTTC CGTGCTCTGG GCACCCCTAA CAACGAAGTG TGGCCTGAGG
     6721 TGGAATCTCT GCAGGACTAC AAGAACACTT TCCCCAAGTG GAAGCCCGGT TCTCTGGCTT
     6781 CCCACGTGAA GAACCTGGAC GAGAACGGAC TGGACCTGCT GTCCAAGATG CTCATCTACG
     6841 ACCCCGCTAA GCGCATCTCC GGCAAGATGG CTCTGAACCA TCCTTACTTC AACGACCTGG
     6901 ACAACCAGAT CAAGAAGATG ATCGCagctg aggctCTGGA AGTTCTGTTT CAGGGACCTC
     6961 AcCAtCATCA CCATCACCAT CACTAAGCTT GTCGAGAAGT ACTAGAGGAT CATAATCAGC
     7021 CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC
     7081 CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT
     7141 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT
     7201 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG ATCACTGCTT
     7261 GAGCCTAGGA GATCCGAACC AGATAAGTGA AATCTAGTTC CAAACTATTT TGTCATTTTT
     7321 AATTTTCGTA TTAGCTTACG ACGCTACACC CAGTTCCCAT CTATTTTGTC ACTCTTCCCT
     7381 AAATAATCCT TAAAAACTCC ATTTCCACCC CTCCCAGTTC CCAACTATTT TGTCCGCCCA
     7441 CAGCGGGGCA TTTTTCTTCC TGTTATGTTT TTAATCAAAC ATCCTGCCAA CTCCATGTGA
     7501 CAAACCGTCA TCTTCGGCTA CTTT
//