LOCUS       MS2_SC2_S_Mu            6264 bp ds-DNA     circular     29-SEP-2021
DEFINITION  .
KEYWORDS    "Antibiotic Resistance"                                            
FEATURES             Location/Qualifiers
     terminator      16..77
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(267..949)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(1044..1841)
                     /label="KanR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        2283..2301
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    2302..2326
                     /label="lac operator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        2335..2364
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     RBS             2384..2406
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2463..3644
                     /label="Maturation Protein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2498..2498
                     /label="g->a (remove BsaI)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3047..3047
                     /label="g->a (remove BspMI)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3293..3293
                     /label="t->c (remove BsmBI)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3668..4057
                     /label="Coat Protein"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3668..4468
                     /label="coat protein dimer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4003..4003
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4061..4063
                     /label="Start of second coat dimer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4100..4117
                     /label="His Tag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4411..4411
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      4482..4543
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4544..4588
                     /label="Spacer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4608..6261
                     /label="insert"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4689..4691
                     /label="R346K"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4902..4904
                     /label="K417N"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5103..5105
                     /label="E484K"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5154..5156
                     /label="N501Y"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5493..5495
                     /label="D614G"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    5694..5696
                     /label="P681H"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 GAGGATCACC CATGTccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
       61 tcttgagggg ttttttgTAA TTTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA
      121 GCTGCATTAA CATGGTCATA GCTGTTTCCT TGCGTATTGG GCGCTCTCCG CTTCCTCGCT
      181 CACTGACTCG CTGCGCTCGG TCGTTCGGGT AAAGCCTGGG GTGCCTAATG AGCAAAAGGC
      241 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
      301 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
      361 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
      421 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
      481 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
      541 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
      601 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
      661 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
      721 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
      781 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
      841 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
      901 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
      961 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     1021 TATGAGTAAA CTTGGTCTGA CAGTTATTAG AAAAATTCAT CCAGCAGACG ATAAAACGCA
     1081 ATACGCTGGC TATCCGGTGC CGCAATGCCA TACAGCACCA GAAAACGATC CGCCCATTCG
     1141 CCGCCCAGTT CTTCCGCAAT ATCACGGGTG GCCAGCGCAA TATCCTGATA ACGATCCGCC
     1201 ACGCCCAGAC GGCCGCAATC AATAAAGCCG CTAAAACGGC CATTTTCCAC CATAATGTTC
     1261 GGCAGGCACG CATCACCATG GGTCACCACC AGATCTTCGC CATCCGGCAT GCTCGCTTTC
     1321 AGACGCGCAA ACAGCTCTGC CGGTGCCAGG CCCTGATGTT CTTCATCCAG ATCATCCTGA
     1381 TCCACCAGGC CCGCTTCCAT ACGGGTACGC GCACGTTCAA TACGATGTTT CGCCTGATGA
     1441 TCAAACGGAC AGGTCGCCGG GTCCAGGGTA TGCAGACGAC GCATGGCATC CGCCATAATG
     1501 CTCACTTTTT CTGCCGGCGC CAGATGGCTA GACAGCAGAT CCTGACCCGG CACTTCGCCC
     1561 AGCAGCAGCC AATCACGGCC CGCTTCGGTC ACCACATCCA GCACCGCCGC ACACGGAACA
     1621 CCGGTGGTGG CCAGCCAGCT CAGACGCGCC GCTTCATCCT GCAGCTCGTT CAGCGCACCG
     1681 CTCAGATCGG TTTTCACAAA CAGCACCGGA CGACCCTGCG CGCTCAGACG AAACACCGCC
     1741 GCATCAGAGC AGCCAATGGT CTGCTGCGCC CAATCATAGC CAAACAGACG TTCCACCCAC
     1801 GCTGCCGGGC TACCCGCATG CAGGCCATCC TGTTCAATCA TACTCTTCCT TTTTCAATAT
     1861 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     1921 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TAAATTGTAA
     1981 GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC
     2041 AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA
     2101 GTGGCCGCTA CAGGGCGCTC CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGT
     2161 TTCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG
     2221 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA
     2281 GGtaatacga ctcactatag gggaattgtg agcggataac aattcccctc tagatttaca
     2341 ctttatgctt ccggctcgta tgtttctaga gaattcaaat aattttgttt aactttaaga
     2401 aggagattta aatTGGCTAT CGCTGTAGGT AGCCGGAATT CCATTCCTAG GAGGTTTGAC
     2461 CTgtgcgagc ttttagtacc cttgataggg agaacgaaac cttcgtcccc tccgttcgcg
     2521 tttacgcgga cggtgagact gaagataact cattctcttt aaaatatcgt tcgaactgga
     2581 ctcccggtcg ttttaactcg actggggcca aaacgaaaca gtggcactac ccctctccgt
     2641 attcacgggg ggcgttaagt gtcacatcga tagatcaagg tgcctacaag cgaagtgggt
     2701 catcgtgggg tcgcccgtac gaggagaaag ccggtttcgg cttctccctc gacgcacgct
     2761 cctgctacag cctcttccct gtaagccaaa acttgactta catcgaagtg ccgcagaacg
     2821 ttgcgaaccg ggcgtcgacc gaagtcctgc aaaaggtcac ccagggtaat tttaaccttg
     2881 gtgttgcttt agcagaggcc aggtcgacag cctcacaact cgcgacgcaa accattgcgc
     2941 tcgtgaaggc gtacactgcc gctcgtcgcg gtaattggcg ccaggcgctc cgctaccttg
     3001 ccctaaacga agatcgaaag tttcgatcaa aacacgtggc cggcagatgg ttggagttgc
     3061 agttcggttg gttaccacta atgagtgata tccagggtgc atatgagatg cttacgaagg
     3121 ttcaccttca agagtttctt cctatgagag ccgtacgtca ggtcggtact aacatcaagt
     3181 tagatggccg tctgtcgtat ccagctgcaa acttccagac aacgtgcaac atatcgcgac
     3241 gtatcgtgat atggttttac ataaacgatg cacgtttggc atggttgtcg tccctaggta
     3301 tcttgaaccc actaggtata gtgtgggaaa aggtgccttt ctcattcgtt gtcgactggc
     3361 tcctacctgt aggtaacatg ctcgagggcc ttacggcccc cgtgggatgc tcctacatgt
     3421 caggaacagt tactgacgta ataacgggtg agtccatcat aagcgttgac gctccctacg
     3481 ggtggactgt ggagagacag ggcactgcta aggcccaaat ctcagccatg catcgagggg
     3541 tacaatccgt atggccaaca actggcgcgt acgtaaagtc tcctttctcg atggtccata
     3601 ccttagatgc gttagcatta atcaggcaac ggctctctag atagAGCCCT CAACCGGAGT
     3661 TTGAAGCATG GCTTCTAACT TTACTCAGTT CGTTCTCGTC GACAATGGCG GAACTGGCGA
     3721 CGTGACTGTC GCCCCAAGCA ACTTCGCTAA CGGGGTCGCT GAATGGATCA GCTCTAACTC
     3781 GCGTTCACAG GCTTACAAAG TAACCTGTAG CGTTCGTCAG AGCTCTGCGC AGAATCGCAA
     3841 ATACACCATC AAAGTCGAGG TGCCTAAAGT GGCAACCCAG ACTGTTGGTG GTGTAGAGCT
     3901 TCCTGTAGCC GCATGGCGTT CGTACTTAAA TATGGAACTA ACCATTCCAA TTTTCGCTAC
     3961 GAATTCCGAC TGCGAGCTTA TTGTTAAGGC AATGCAAGGT CTTCTAAAAG ATGGAAACCC
     4021 GATTCCCTCA GCAATCGCAG CAAACTCCGG CATCTACGCT AACTTTACTC AGTTCGTTCT
     4081 CGTCGACAAT GGCGGTACCC ATCACCATCA CCATCACGGT ACCGGCGACG TGACTGTCGC
     4141 CCCAAGCAAC TTCGCTAACG GGGTCGCTGA ATGGATCAGC TCTAACTCGC GTTCACAGGC
     4201 TTACAAAGTA ACCTGTAGCG TtCGTCAGAG CTCTGCGCAG AATCGCAAAT ACACCATCAA
     4261 AGTCGAGGTG CCTAAAGTGG CAACCCAGAC TGTTGGTGGT GTAGAGCTTC CTGTAGCCGC
     4321 ATGGCGTTCG TACTTAAATA TGGAACTAAC CATTCCAATT TTCGCTACGA ATTCCGACTG
     4381 CGAGCTTATT GTTAAGGCAA TGCAAGGTCT TCTAAAAGAT GGAAACCCGA TTCCCTCAGC
     4441 AATCGCAGCA AACTCCGGCA TCTACTAATA GACGCCGGCC Accgctgagc aataactagc
     4501 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgTCGAACA GAAAGTAATC
     4561 GTATTGTACA CGGCCGCATA ATCGAAATTA ATACGACTCA CTATAGGaga gtccaaccaa
     4621 cagaatctat tgttagattt cctaatatta caaacttgtg cccttttggt gaagttttta
     4681 acgccaccAA Atttgcatct gtttatgctt ggaacaggaa gagaatcagc aactgtgttg
     4741 ctgattattc tgtcctatat aattccgcat cattttccac ttttaagtgt tatggagtgt
     4801 ctcctactaa attaaatgat ctctgcttta ctaatgtcta tgcagattca tttgtaatta
     4861 gaggtgatga agtcagacaa atcgctccag ggcaaactgg aaatattgct gattataatt
     4921 ataaattacc agatgatttt acaggctgcg ttatagcttg gaattctaac aatcttgatt
     4981 ctaaggttgg tggtaattat aattacctgt atagattgtt taggaagtct aatctcaaac
     5041 cttttgagag agatatttca actgaaatct atcaggccgg tagcacacct tgtaatggtg
     5101 ttAAAggttt taattgttac tttcctttac aatcatatgg tttccaaccc acttatggtg
     5161 ttggttacca accatacaga gtagtagtac tttcttttga acttctacat gcaccagcaa
     5221 ctgtttgtgg acctaaaaag tctactaatt tggttaaaaa caaatgtgtc aatttcaact
     5281 tcaatggttt aacaggcaca ggtgttctta ctgagtctaa caaaaagttt ctgcctttcc
     5341 aacaatttgg cagagacatt gctgacacta ctgatgctgt ccgtgatcca cagacacttg
     5401 agattcttga cattacacca tgttcttttg gtggtgtcag tgttataaca ccaggaacaa
     5461 atacttctaa ccaggttgct gttctttatc agggtgttaa ctgcacagaa gtccctgttg
     5521 ctattcatgc agatcaactt actcctactt ggcgtgttta ttctacaggt tctaatgttt
     5581 ttcaaacacg tgcaggctgt ttaatagggg ctgaacatgt caacaactca tatgagtgtg
     5641 acatacccat tggtgcaggt atatgcgcta gttatcagac tcagactaat tctcatcggc
     5701 gggcacgtag tgtagctagt caatccatca ttgcctacac tatgtcactt ggtgcagaaa
     5761 attcagttgc ttactctaat aactctattg ccatacccac aaattttact attagtgtta
     5821 ccacagaaat tctaccagtg tctatgacca agacatcagt agattgtaca atgtacattt
     5881 gtggtgattc aactgaatgc agcaatcttt tgttgcaata tggcagtttt tgtacacaat
     5941 taaaccgtgc tttaactgga atagctgttg aacaagacaa aaacacccaa gaagtttttg
     6001 cacaagtcaa acaaatttac aaaacaccac caattaaaga ttttggtggt tttaattttt
     6061 cacaaatatt accagatcca tcaaaaccaa gcaagaggtc atttattgaa gatctacttt
     6121 tcaacaaagt gacacttgca gatgctggct tcatcaaaca atatggtgat tgccttggtg
     6181 atattgctgc tagagacctc atttgtgcac aaaagtttaa cggccttact gttttgccac
     6241 ctttgctcac agatgaaatg aCAT
//