LOCUS       MS2_SC2_S_C1.2          6264 bp ds-DNA     circular     17-SEP-2021
DEFINITION  .
KEYWORDS    "Antibiotic Resistance"                                            
FEATURES             Location/Qualifiers
     promoter        4..33
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     RBS             53..75
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             132..1313
                     /label="Maturation Protein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    167..167
                     /label="g->a (remove BsaI)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    716..716
                     /label="g->a (remove BspMI)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    962..962
                     /label="t->c (remove BsmBI)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1337..1726
                     /label="Coat Protein"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1337..2137
                     /label="coat protein dimer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1672..1672
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1730..1732
                     /label="Start of second coat dimer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1769..1786
                     /label="His Tag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2080..2080
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2151..2212
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2213..2257
                     /label="Spacer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2277..3930
                     /label="insert"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2667..2669
                     /label="Y449H"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2772..2774
                     /label="E484K"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2823..2825
                     /label="N501Y"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3162..3164
                     /label="D614G"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3285..3287
                     /label="H655Y"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3357..3359
                     /label="N679K"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3468..3470
                     /label="T716I"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3897..3899
                     /label="T859N"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      3949..4010
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(4200..4882)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(4977..5774)
                     /label="KanR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        6216..6234
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    6235..6259
                     /label="lac operator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 agatttacac tttatgcttc cggctcgtat gtttctagag aattcaaata attttgttta
       61 actttaagaa ggagatttaa atTGGCTATC GCTGTAGGTA GCCGGAATTC CATTCCTAGG
      121 AGGTTTGACC Tgtgcgagct tttagtaccc ttgataggga gaacgaaacc ttcgtcccct
      181 ccgttcgcgt ttacgcggac ggtgagactg aagataactc attctcttta aaatatcgtt
      241 cgaactggac tcccggtcgt tttaactcga ctggggccaa aacgaaacag tggcactacc
      301 cctctccgta ttcacggggg gcgttaagtg tcacatcgat agatcaaggt gcctacaagc
      361 gaagtgggtc atcgtggggt cgcccgtacg aggagaaagc cggtttcggc ttctccctcg
      421 acgcacgctc ctgctacagc ctcttccctg taagccaaaa cttgacttac atcgaagtgc
      481 cgcagaacgt tgcgaaccgg gcgtcgaccg aagtcctgca aaaggtcacc cagggtaatt
      541 ttaaccttgg tgttgcttta gcagaggcca ggtcgacagc ctcacaactc gcgacgcaaa
      601 ccattgcgct cgtgaaggcg tacactgccg ctcgtcgcgg taattggcgc caggcgctcc
      661 gctaccttgc cctaaacgaa gatcgaaagt ttcgatcaaa acacgtggcc ggcagatggt
      721 tggagttgca gttcggttgg ttaccactaa tgagtgatat ccagggtgca tatgagatgc
      781 ttacgaaggt tcaccttcaa gagtttcttc ctatgagagc cgtacgtcag gtcggtacta
      841 acatcaagtt agatggccgt ctgtcgtatc cagctgcaaa cttccagaca acgtgcaaca
      901 tatcgcgacg tatcgtgata tggttttaca taaacgatgc acgtttggca tggttgtcgt
      961 ccctaggtat cttgaaccca ctaggtatag tgtgggaaaa ggtgcctttc tcattcgttg
     1021 tcgactggct cctacctgta ggtaacatgc tcgagggcct tacggccccc gtgggatgct
     1081 cctacatgtc aggaacagtt actgacgtaa taacgggtga gtccatcata agcgttgacg
     1141 ctccctacgg gtggactgtg gagagacagg gcactgctaa ggcccaaatc tcagccatgc
     1201 atcgaggggt acaatccgta tggccaacaa ctggcgcgta cgtaaagtct cctttctcga
     1261 tggtccatac cttagatgcg ttagcattaa tcaggcaacg gctctctaga tagAGCCCTC
     1321 AACCGGAGTT TGAAGCATGG CTTCTAACTT TACTCAGTTC GTTCTCGTCG ACAATGGCGG
     1381 AACTGGCGAC GTGACTGTCG CCCCAAGCAA CTTCGCTAAC GGGGTCGCTG AATGGATCAG
     1441 CTCTAACTCG CGTTCACAGG CTTACAAAGT AACCTGTAGC GTTCGTCAGA GCTCTGCGCA
     1501 GAATCGCAAA TACACCATCA AAGTCGAGGT GCCTAAAGTG GCAACCCAGA CTGTTGGTGG
     1561 TGTAGAGCTT CCTGTAGCCG CATGGCGTTC GTACTTAAAT ATGGAACTAA CCATTCCAAT
     1621 TTTCGCTACG AATTCCGACT GCGAGCTTAT TGTTAAGGCA ATGCAAGGTC TTCTAAAAGA
     1681 TGGAAACCCG ATTCCCTCAG CAATCGCAGC AAACTCCGGC ATCTACGCTA ACTTTACTCA
     1741 GTTCGTTCTC GTCGACAATG GCGGTACCCA TCACCATCAC CATCACGGTA CCGGCGACGT
     1801 GACTGTCGCC CCAAGCAACT TCGCTAACGG GGTCGCTGAA TGGATCAGCT CTAACTCGCG
     1861 TTCACAGGCT TACAAAGTAA CCTGTAGCGT tCGTCAGAGC TCTGCGCAGA ATCGCAAATA
     1921 CACCATCAAA GTCGAGGTGC CTAAAGTGGC AACCCAGACT GTTGGTGGTG TAGAGCTTCC
     1981 TGTAGCCGCA TGGCGTTCGT ACTTAAATAT GGAACTAACC ATTCCAATTT TCGCTACGAA
     2041 TTCCGACTGC GAGCTTATTG TTAAGGCAAT GCAAGGTCTT CTAAAAGATG GAAACCCGAT
     2101 TCCCTCAGCA ATCGCAGCAA ACTCCGGCAT CTACTAATAG ACGCCGGCCA ccgctgagca
     2161 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgTCGAACAG
     2221 AAAGTAATCG TATTGTACAC GGCCGCATAA TCGAAATTAA TACGACTCAC TATAGGagag
     2281 tccaaccaac agaatctatt gttagatttc ctaatattac aaacttgtgc ccttttggtg
     2341 aagtttttaa cgccaccaga tttgcatctg tttatgcttg gaacaggaag agaatcagca
     2401 actgtgttgc tgattattct gtcctatata attccgcatc attttccact tttaagtgtt
     2461 atggagtgtc tcctactaaa ttaaatgatc tctgctttac taatgtctat gcagattcat
     2521 ttgtaattag aggtgatgaa gtcagacaaa tcgctccagg gcaaactgga aagattgctg
     2581 attataatta taaattacca gatgatttta caggctgcgt tatagcttgg aattctaaca
     2641 atcttgattc taaggttggt ggtaatcata attacctgta tagattgttt aggaagtcta
     2701 atctcaaacc ttttgagaga gatatttcaa ctgaaatcta tcaggccggt agcacacctt
     2761 gtaatggtgt taaaggtttt aattgttact ttcctttaca atcatatggt ttccaaccca
     2821 cttatggtgt tggttaccaa ccatacagag tagtagtact ttcttttgaa cttctacatg
     2881 caccagcaac tgtttgtgga cctaaaaagt ctactaattt ggttaaaaac aaatgtgtca
     2941 atttcaactt caatggttta acaggcacag gtgttcttac tgagtctaac aaaaagtttc
     3001 tgcctttcca acaatttggc agagacattg ctgacactac tgatgctgtc cgtgatccac
     3061 agacacttga gattcttgac attacaccat gttcttttgg tggtgtcagt gttataacac
     3121 caggaacaaa tacttctaac caggttgctg ttctttatca gggtgttaac tgcacagaag
     3181 tccctgttgc tattcatgca gatcaactta ctcctacttg gcgtgtttat tctacaggtt
     3241 ctaatgtttt tcaaacacgt gcaggctgtt taataggggc tgaatatgtc aacaactcat
     3301 atgagtgtga catacccatt ggtgcaggta tatgcgctag ttatcagact cagactaaat
     3361 ctcctcggcg ggcacgtagt gtagctagtc aatccatcat tgcctacact atgtcacttg
     3421 gtgcagaaaa ttcagttgct tactctaata actctattgc catacccata aattttacta
     3481 ttagtgttac cacagaaatt ctaccagtgt ctatgaccaa gacatcagta gattgtacaa
     3541 tgtacatttg tggtgattca actgaatgca gcaatctttt gttgcaatat ggcagttttt
     3601 gtacacaatt aaaccgtgct ttaactggaa tagctgttga acaagacaaa aacacccaag
     3661 aagtttttgc acaagtcaaa caaatttaca aaacaccacc aattaaagat tttggtggtt
     3721 ttaatttttc acaaatatta ccagatccat caaaaccaag caagaggtca tttattgaag
     3781 atctactttt caacaaagtg acacttgcag atgctggctt catcaaacaa tatggtgatt
     3841 gccttggtga tattgctgct agagacctca tttgtgcaca aaagtttaac ggccttaatg
     3901 ttttgccacc tttgctcaca gatgaaatga CATGAGGATC ACCCATGTcc gctgagcaat
     3961 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg TAATTTCACT
     4021 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAACATGGTC ATAGCTGTTT
     4081 CCTTGCGTAT TGGGCGCTCT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG
     4141 GGTAAAGCCT GGGGTGCCTA ATGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
     4201 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
     4261 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
     4321 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
     4381 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
     4441 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
     4501 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
     4561 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
     4621 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
     4681 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
     4741 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
     4801 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     4861 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     4921 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAT
     4981 TAGAAAAATT CATCCAGCAG ACGATAAAAC GCAATACGCT GGCTATCCGG TGCCGCAATG
     5041 CCATACAGCA CCAGAAAACG ATCCGCCCAT TCGCCGCCCA GTTCTTCCGC AATATCACGG
     5101 GTGGCCAGCG CAATATCCTG ATAACGATCC GCCACGCCCA GACGGCCGCA ATCAATAAAG
     5161 CCGCTAAAAC GGCCATTTTC CACCATAATG TTCGGCAGGC ACGCATCACC ATGGGTCACC
     5221 ACCAGATCTT CGCCATCCGG CATGCTCGCT TTCAGACGCG CAAACAGCTC TGCCGGTGCC
     5281 AGGCCCTGAT GTTCTTCATC CAGATCATCC TGATCCACCA GGCCCGCTTC CATACGGGTA
     5341 CGCGCACGTT CAATACGATG TTTCGCCTGA TGATCAAACG GACAGGTCGC CGGGTCCAGG
     5401 GTATGCAGAC GACGCATGGC ATCCGCCATA ATGCTCACTT TTTCTGCCGG CGCCAGATGG
     5461 CTAGACAGCA GATCCTGACC CGGCACTTCG CCCAGCAGCA GCCAATCACG GCCCGCTTCG
     5521 GTCACCACAT CCAGCACCGC CGCACACGGA ACACCGGTGG TGGCCAGCCA GCTCAGACGC
     5581 GCCGCTTCAT CCTGCAGCTC GTTCAGCGCA CCGCTCAGAT CGGTTTTCAC AAACAGCACC
     5641 GGACGACCCT GCGCGCTCAG ACGAAACACC GCCGCATCAG AGCAGCCAAT GGTCTGCTGC
     5701 GCCCAATCAT AGCCAAACAG ACGTTCCACC CACGCTGCCG GGCTACCCGC ATGCAGGCCA
     5761 TCCTGTTCAA TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     5821 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     5881 ACATTTCCCC GAAAAGTGCC ACCTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT
     5941 TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT
     6001 ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGGCCG CTACAGGGCG CTCCCATTCG
     6061 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGTTTCGGTG CGGGCCTCTT CGCTATTACG
     6121 CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC
     6181 CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAGGtaata cgactcacta taggggaatt
     6241 gtgagcggat aacaattccc ctct
//