LOCUS       Collins_T7-sensor_1_[sT 3956 bp ds-DNA     circular     04-AUG-2021
DEFINITION  .
KEYWORDS    "creator:MK"                                                       
FEATURES             Location/Qualifiers
     terminator      complement(1..67)
                     /label="T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        97..115
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          116..151
                     /label="lin_toehold_engi_P1_add_FW"
                     /note="sequence: gggtgagctaacaccgtgcgtgttgacaattttacctctggcggtgataatggttgcaagcaGGGTCTTATCTTATCTATCTCGTTTATCCCTG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          120..195
                     /label="P1-loop-rbs-56"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaacagaggagatATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     misc_feature    123..152
                     /label="Trigger Binding region"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    156..170
                     /label="LOOP with RBS"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          163..195
                     /label="P1-loop-rbs-56-c-to-g"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagagaggagatATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          165..195
                     /label="P1-J61106"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagataggagatATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          166..195
                     /label="P1-J61109"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagactggagatATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          170..195
                     /label="P1-J61108"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagacgagatatATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          170..195
                     /label="P1-J61103"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagattagacatATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          170..195
                     /label="P1-J61104"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagaagggacatATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          170..195
                     /label="P1-J61100"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagaggggacatATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          171..195
                     /label="P1-J61102"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagatccgatgtATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          171..195
                     /label="P1-J61105"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagacatgacgtATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          171..195
                     /label="P1-J61107"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagaagagacttATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          171..195
                     /label="P1-J61101"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagacaggacctATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          171..195
                     /label="P1-J61110"
                     /note="sequence: GGGTCTTATCTTATCTATCTCGTTTATCCCTGCAtacagaaagaggcgaattATGCAATGATAAACGAGAACCTGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     misc_feature    177..179
                     /label="START CODON"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    189..209
                     /label="21 nt linker region"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             210..213
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#d20ff7
                     /ApEinfo_fwdcolor=#d20ff7
     misc_feature    210..213
                     /label="Sequenced CIJ510-13 Clone +1,+2#"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             214..965
                     /label="(-Met) sfGFP (+ASV)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             214..965
                     /label="Translation 214-965"
     misc_feature    930..962
                     /label="ASV degradation tag"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     terminator      969..1016
                     /label="T7 terminator SBa_000587"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer          complement(997..1016)
                     /label="SBa_000587_RV"
                     /note="sequence: acaaaaaacccctagccgcc"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer_bind     1031..1054
                     /label="F24"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     terminator      complement(1061..1163)
                     /label="T0 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1395..2186
                     /label="spectinomycin/streptomycin resistance gene"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    complement(2215..2419)
                     /label="OriT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      2451..3924
                     /label="PSC101"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(2631..3581)
                     /label="RepA"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
ORIGIN
        1 TCCTACTCAG GAGAGCGTTC ACCGACAAAC AACAGATAAA ACGAAAGGCC CAGTCTTTCG
       61 ACTGAGCCTT TCGTTTTATT TGATGCCTTT AATTAAtaat acgactcact ataggagcaG
      121 GGTCTTATCT TATCTATCTC GTTTATCCCT GCATACAGAA ACAGAGGAGA TATGCAATGA
      181 TAAACGAGAA CCTGGCGGCA GCGCAAAAGa tgcGTAAAGG CGAAGAACTG TTTACCGGTG
      241 TGGTTCCGAT TCTGGTGGAA CTGGACGGCG ATGTTAATGG TCATAAATTC AGTGTTCGCG
      301 GCGAAGGTGA AGGCGATGCG ACGAACGGCA AACTGACCCT GAAATTTATC TGCACCACGG
      361 GTAAACTGCC GGTCCCGTGG CCGACGCTGG TGACCACGCT GACCTATGGC GTTCAATGTT
      421 TTGCGCGTTA CCCGGATCAC ATGAAACAGC ACGACTTTTT CAAATCGGCC ATGCCGGAAG
      481 GCTATGTGCA GGAACGTACG ATTAGCTTTA AAGACGATGG TACGTATAAA ACCCGCGCGG
      541 AAGTGAAATT CGAAGGCGAT ACCCTGGTTA ACCGTATCGA ACTGAAAGGT ATCGATTTCA
      601 AAGAAGACGG CAATATTCTG GGTCATAAAC TGGAATATAA CTTCAATTCC CACAACGTGT
      661 ACATCACCGC GGATAAACAG AAAAACGGCA TTAAAGCCAA TTTCAAAATC CGCCATAATG
      721 TGGAAGATGG TAGCGTTCAG CTGGCCGACC ACTATCAGCA AAACACGCCG ATTGGTGATG
      781 GCCCGGTCCT GCTGCCGGAC AATCACTACC TGAGTACCCA GTCCGTGCTG TCAAAAGATC
      841 CGAACGAAAA ACGTGACCAC ATGGTCCTGC TGGAATTTGT GACGGCTGCG GGTATCACCC
      901 ACGGCATGGA CGAACTGTAT AAAAGGCCTg cagcaaacga cgaaaactac gctgcatcag
      961 ttTAATAAta ctcgaacccc tagcccgctc ttatcgggcg gctaggggtt ttttgtCCTA
     1021 GGGCGGCCGC GTCGTGACTG GGAAAACCCT GGCGACTAGT CTTGGACTCC TGTTGATAGA
     1081 TCCAGTAATG ACCTCAGAAC TCCATCTGGA TTTGTTCAGA ACGCTCGGTT GCCGCCGGGC
     1141 GTTTTTTATT GGTGAGAATC CAGGGGTCCC CAATAATTAC GATTTACGTA TTTAAATgaa
     1201 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1261 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     1321 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     1381 caaagttaaa catcATGAGG GAAGCGGTGA TCGCCGAAGT ATCGACTCAA CTATCAGAGG
     1441 TAGTTGGCGT CATCGAGCGC CATCTCGAAC CGACGTTGCT GGCCGTACAT TTGTACGGCT
     1501 CCGCAGTGGA TGGCGGCCTG AAGCCACACA GTGATATTGA TTTGCTGGTT ACGGTGACCG
     1561 TAAGGCTTGA TGAAACAACG CGGCGAGCTT TGATCAACGA CCTTTTGGAA ACTTCGGCTT
     1621 CCCCTGGAGA GAGCGAGATT CTCCGCGCTG TAGAAGTCAC CATTGTTGTG CACGACGACA
     1681 TCATTCCGTG GCGTTATCCA GCTAAGCGCG AACTGCAATT TGGAGAATGG CAGCGCAATG
     1741 ACATTCTTGC AGGTATCTTC GAGCCAGCCA CGATCGACAT TGATCTGGCT ATCTTGCTGA
     1801 CAAAAGCAAG AGAACATAGC GTTGCCTTGG TAGGTCCAGC GGCGGAGGAA CTCTTTGATC
     1861 CGGTTCCTGA ACAGGATCTA TTTGAGGCGC TAAATGAAAC CTTAACGCTA TGGAACTCGC
     1921 CGCCCGACTG GGCTGGCGAT GAGCGAAATG TAGTGCTTAC GTTGTCCCGC ATTTGGTACA
     1981 GCGCAGTAAC CGGCAAAATC GCGCCGAAGG ATGTCGCTGC CGACTGGGCA ATGGAGCGCC
     2041 TGCCGGCCCA GTATCAGCCC GTCATACTTG AAGCTAGACA GGCTTATCTT GGACAAGAAG
     2101 AAGATCGCTT GGCCTCGCGC GCAGATCAGT TGGAAGAATT TGTCCACTAC GTGAAAGGCG
     2161 AGATCACCAA GGTAGTCGGC AAATAAGACa aTTGTCCTTT TCCGCTGCAT AACCCTGCTT
     2221 CGGGGTCATT ATAGCGATTT TTTCGGTATA TCCATCCTTT TTCGCACGAT ATACAGGATT
     2281 TTGCCAAAGG GTTCGTGTAG ACTTTCCTTG GTGTATCCAA CGGCGTCAGC CGGGCAGGAT
     2341 AGGTGAAGTA GGCCCACCCG CGAGCGGGTG TTCCTTCTTC ACTGTCCCTT ATTCGCACCT
     2401 GGCGGTGCTC AACGGGAATC CTGCTCTGCG AGGCTGGCCG TAGGCCGGCC tggtgaacag
     2461 ctttgaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt
     2521 catctgtgca tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt
     2581 tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt
     2641 ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac
     2701 gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg
     2761 agctgaattt ttgctgttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag
     2821 gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca
     2881 agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc
     2941 gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt
     3001 attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt
     3061 aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt
     3121 gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta
     3181 acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg caatatctct
     3241 tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaag
     3301 atcttaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg
     3361 gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat
     3421 tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg
     3481 agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta
     3541 atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg
     3601 tgattttaat cactatacca attgagatgg gctagtcaat gataattact agtccttttc
     3661 ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct
     3721 gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg
     3781 gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct
     3841 gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg
     3901 tttgctcctc tacaaaacag acctGGCGCG CCCAGCTGTC TAGGGCGGCG GATTTG
//