LOCUS       pSSa23                  8404 bp    DNA     circular UNA 01-OCT-2021
DEFINITION  length=8404 depth=1.00x circular=true.
ACCESSION   urn.local.dsanglar.4d-dx8lte1
VERSION     urn.local.dsanglar.4d-dx8lte1
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             62..586
                     /vntifkey=4
                     /modified_by="User"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSSa23"
                     /Transferred_Similarity="100.00%"
                     /Adjusted_Transferred_Similarity="99.43%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/-3
                     (adjusted to 99.43%; Adjusted-Interval-0:0/0); SAT1 (<a
                     href=""urn:local:dsanglar:k-dx83xvf"">pSSa23</a>)"
                     /label="SAT1"
     terminator      587..716
                     /created_by="dsanglar"
                     /modified_by="dsanglar"
                     /label="URA3t"
     rep_origin      1175..1774
                     /%_Identity=100
                     /Motif="GGGGATCTCTAATGATGCAATTTTTGTTTTGTACACTAAGATTCTATTTC
                     GGAACTCTTACCCATGTCATTATTTACTTTATTTTCATTATTGTAATTTTTTAAGCTT
                     TTATTATGAATTATTTAAATACAATTCTTTTTGATCATGTGACAGACATCACTGAAAG
                     ATGTGCTCTGCGTTATTAGTCAGATTGAAATATACATTTTCATGTAGATATGATGTTC
                     TTACTTTAAATGTTGTTCCATTTGCTAGCTTAAATAGACGATATTATAAATTTAAGTA
                     TTCACTAAAAATCTGACCCATTAATGTTAGAATTAGTATTATTTTTTGTGATATTACA
                     TTTTTATATTTTGGATTTCAACCAATGCTCTGCGATATGATTCAACAGAATAATATGT
                     ACAAGCTACTTTAATTCATATATACTAATAGATTCACAGATCCAGTACGACTGCAAGA
                     ATTTACGTTTTGTTTGAAATGGTAGTAGCTACACTAAATTAACAATGATCGATTAATC
                     CACATCCACAGTGTGTAATCATCCTAATGAAAACAAGAATAACTACAAAAATGAATAT
                     AAACTTTTTGCATGTTAGTCTAACCTGC"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSSa23"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     CgCEN8/ARS (<a
                     href=""urn:local:dsanglar:k-dx83xvf"">pSSa23</a>)"
                     /modified_by="dsanglar"
                     /label="CgCEN/ARS"
     CDS             1848..2708
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="TTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTC
                     GTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTT
                     ACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGAT
                     TTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTT
                     TATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCC
                     AGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCG
                     TCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGAT
                     CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAG
                     TAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACT
                     GTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCT
                     GAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATAC
                     CGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGA
                     AAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCAC
                     CCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGG
                     AAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATA
                     CTCTTCCTTTTT"
                     /modified_by="User"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSSa23"
                     /Transferred_Similarity="100.00%"
                     /Adjusted_Transferred_Similarity="98.51%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:-13/0
                     (adjusted to 98.51%; Adjusted-Interval-0:0/0); AmpR (<a
                     href=""urn:local:dsanglar:k-dx83xvf"">pSSa23</a>)"
                     /label="AmpR"
     promoter        3921..4455
                     /created_by="dsanglar"
                     /label="pTEF1"
     gene            4459..6111
                     /created_by="sschreve"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSSa23"
                     /Transferred_Similarity="99.70%"
                     /Primary_Match="99.70%; Adjusted-Interval-0:0/0;
                     CgLUCopt-mut2 (eurofins) (<a
                     href=""urn:local:dsanglar:k-dx83xvf"">pSSa23</a>)"
                     /modified_by="dsanglar"
                     /label="CgLUCopt-mut2"
     promoter        7267..61
                     /vntifkey=52
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSSa23"
                     /Transferred_Similarity="99.92%"
                     /Primary_Match="99.92%; Adjusted-Interval-0:0/0; ACT1p (<a
                     href=""urn:local:dsanglar:k-dx83xvf"">pSSa23</a>)"
                     /modified_by="dsanglar"
                     /label="ACT1p"
ORIGIN      
        1 gtcatactaa cccattttta tagaagaagt tgctgcttta gttatcgata acggttctca
       61 tatgaaaatt tcggtgatcc ctgagcaggt ggcggaaaca ttggatgctg agaaccattt
      121 cattgttcgt gaagtgttcg atgtgcacct atccgaccaa ggctttgaac tatctaccag
      181 aagtgtgagc ccctaccgga aggattacat ctcggatgat gactctgatg aagactctgc
      241 ttgctatggc gcattcatcg accaagagct tgtcgggaag attgaactca actcaacatg
      301 gaacgatcta gcctctatcg aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt
      361 cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta agcagacagc tccttggcat
      421 acgattagag acacaaacga acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt
      481 tactctcggc ggcattgacc tcttcacgta taaaactaga cctcaagtct cgaacgaaac
      541 agcgatgtac tggtactggt tctcgggagc acaggatgac gcctaacata tgtgaagtgt
      601 gaagggggag attttcactt tattagattt gtatatatgt ataataaata aataaataag
      661 ttaaataaat aattagataa gggtggtaat tattactatt tacaatcaaa ggtggtcctg
      721 caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca
      781 ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt
      841 tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa
      901 aaaaatgatg aattgaaaag gtggtatggt gcactctcag tacaatctgc tctgatgccg
      961 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     1021 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1081 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     1141 tataggttaa tgtcatgata ataatggttt cttagcaggt tagactaaca tgcaaaaagt
     1201 ttatattcat ttttgtagtt attcttgttt tcattaggat gattacacac tgtggatgtg
     1261 gattaatcga tcattgttaa tttagtgtag ctactaccat ttcaaacaaa acgtaaattc
     1321 ttgcagtcgt actggatctg tgaatctatt agtatatatg aattaaagta gcttgtacat
     1381 attattctgt tgaatcatat cgcagagcat tggttgaaat ccaaaatata aaaatgtaat
     1441 atcacaaaaa ataatactaa ttctaacatt aatgggtcag atttttagtg aatacttaaa
     1501 tttataatat cgtctattta agctagcaaa tggaacaaca tttaaagtaa gaacatcata
     1561 tctacatgaa aatgtatatt tcaatctgac taataacgca gagcacatct ttcagtgatg
     1621 tctgtcacat gatcaaaaag aattgtattt aaataattca taataaaagc ttaaaaaatt
     1681 acaataatga aaataaagta aataatgaca tgggtaagag ttccgaaata gaatcttagt
     1741 gtacaaaaca aaaattgcat cattagagat ccccacattc aaatatgtat ccgctcatga
     1801 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1861 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1921 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1981 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2041 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2101 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2161 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2221 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2281 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2341 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2401 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2581 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2761 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2821 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3001 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3481 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3541 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3601 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3661 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3721 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     3781 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     3841 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     3901 actaaaggga acaaaagctg gagctcctat accaacatgc caaaccttat gaagagtctt
     3961 caaccaaacc tacaatagat gactacagcg ctagcatcat aagtagatag gtcaccaacc
     4021 aagtgatgaa gtttgcgtta cggcactggt aaccagctct tgaattgctt ttccaggaaa
     4081 agaaacgtca tataatacag accatacaga caatacagat gggggaccct ctcataatgg
     4141 ccagcatcaa cattagtagt tgtaaggagt acccctgtaa gtgctgcttc ctagttggcc
     4201 accgttgaga gagtacctcc tccctcactt attgctatta ttgcttttta ttgtttcttt
     4261 tttcaactga aaaatttttt tttttcaatg cgatgagttt gaagaaaaaa tttgaaaaaa
     4321 attcatcgat ggaataagat atttaagcag cttgggtttt taaaacttct cttgaaaact
     4381 ttccacttct tgttaaattc ctttttgtag aaagttcagc acagataact aacttgaact
     4441 aatatagcag gatccaaaat ggaggatgct aagaacatta agaagggccc tgcccctttc
     4501 tatccattag aagatggtac agcaggtgaa caacttcaca aagctatgaa gagatacgct
     4561 ttagtcccag gtactattgc cttcactgat gcccatatag aggttgacat aacatacgcg
     4621 gagtacttcg agatgtcagt gagattagcg gaagccatga agagatatgg cttgaatact
     4681 aatcacagga ttgtggtttg ctctgaaaac tcattgcagt tctttatgcc agttttgggt
     4741 gcattattca ttggtgtcgc tgttgctcca gcaaatgata tatacaacga aagagaatta
     4801 ttgaactcta tggggatctc tcaaccaact gtcgtgtttg tcagcaagaa gggattgcaa
     4861 aagattctta acgttcagaa gaaacttccc atcattcaga agatcatcat catggacagc
     4921 aaaaccgact atcaaggctt ccaatcaatg tatacctttg ttacatctca cctaccacct
     4981 gggtttaacg agtatgactt tgtaccagag tctttcgata gagataagac cattgctctg
     5041 atcatgaatt cctctggtag tactggtcta ccaaagggtg ttgctctgcc acatagagca
     5101 ttatgtgtca gatttagcca tgctagagat cccatatttg gtaatcaaat tattccagac
     5161 actgccattc tgtccgtcgt tcctttccac catggttttg ggatgttcac aaccttgggt
     5221 tacctaatct gtggttttag agttatgttg atgtatcgtt ttgaagaaga gttgttcttg
     5281 agatcattgc aagactacaa aatacagaca gccctgttgg tcccaacact gttctctttc
     5341 ttggctaaga gtaccctaat agacaagtac gatttgagta acctacacga aatcgcatct
     5401 ggaggtgcac cactttccaa agaggttggt gaagctgtcg ccaaaagatt tcacttacct
     5461 ggcattagac aaggttatgg cctgacagag actactagtg ctatactgat tacccctgaa
     5521 ggagatgata aacccggtgc agttggcaaa gtggtcccat tctttgaagc aaaagttgta
     5581 gacttagata ctggcaaaac tcttggagtg aatcaacgtg gtgagttatg tgtacgtggt
     5641 ccaatgatta tgtccggcta cgtcaacaat ccagaagcaa caaatgcctt aatagacaaa
     5701 gatggatggc tacattccgg tgatatagcc tattgggatg aggacgaaca cttcttcatc
     5761 gtagataggc ttaagtcttt gatcaaatac aagggttatc aagttgctcc tgctgaattg
     5821 gagtccattc tactacagca tcctaacatc tttgatgctg gtgttgcagg tttaccagac
     5881 gacgatgctg gtgaacttcc agcggctgtg gttgtgttgg aacatggtaa aaccatgact
     5941 gagaaggaaa tcgttgacta cgtagcttca caagtaacta ccgcaaagaa actaagaggt
     6001 ggagtagtct ttgtagacga agttccaaag ggtttgacag gcaaattgga tgccagaaag
     6061 attagggaaa tcttgattaa agctaagaaa ggtggcaaga ttgctgttta aggtacccaa
     6121 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     6181 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6241 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     6301 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6361 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6421 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6481 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6541 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6601 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6661 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6721 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
     6781 ttctccttac gcatctgtgc ggtatttcac accgcatagg gtaataactg atataattaa
     6841 attgaagctc taatttgtga gtttagtata catgcattta cttataatac agttttttag
     6901 ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc
     6961 tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat
     7021 cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca
     7081 tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg
     7141 tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta
     7201 cccttagtat attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa
     7261 aggcctgtca aaactagaga ataataaaga aaacgatctt ttcaaaaaga aaaaaccttt
     7321 tagttttcct ttgttgttgt tgtgggtgtg tgctatttat attatatagt ttactcataa
     7381 taccataaaa tattcggttt gattaggtta ttttaataag ctaatttgtt tctaatcgtg
     7441 taatttatgc tgtgtatatt aagtagtgtg tgcactgccc aaaaatgttt gttgtttata
     7501 gtcggttaaa gagaaaaaag aaaaaaagat ccatacacac acgttaatta gttgttcaac
     7561 gtaatacact catattttgt tcttatttgc tttcggtcgc tgttctcacc aagatttatt
     7621 gccaacgaaa caattttttt ttatatattt tcagattttt ctttttttcc tttcctttcc
     7681 ttttctaatt ttcactcctg gttttctttc tttcttagaa acattatctc gatattaata
     7741 ttaaaaaaat ataatcattc aaaatggacg gtggtatgtt ttagtttagc ttcaattcta
     7801 attgattgat taatcagttg attggtttca atatgacaaa tgggtagggt gggaaaactt
     7861 cattttcaat tcagatcaaa cttttttgtt gtcgacataa tatttctcgt ttgggatgtt
     7921 actgtcacat taataataca cacacatcag cttataattt tgaaagtaat ttatcagata
     7981 tgttgtgacg atcagtggaa atggctaact tcaatgtatc tgttcttccc ctttttcaaa
     8041 gttcacgttt tttgattgat tgattgatct gtcggcagtg gtttcaaaac cattcggtga
     8101 gtaatcctat caatcaatgt tacgacaaaa ggctcaatat tcaaaattgc aatgttttat
     8161 gttttcctac gtgtacttgt gcaaggcaat tgattcaaca ttgcttttgg tgtttgacga
     8221 gtttctagtt tggacttgtg ttgttatctg ggctatacag atttcccggc tcactatgaa
     8281 tttttttttt cgacgctcag tgcacacaac tataaacaac acaaacacaa acacagcaag
     8341 aaaaaaaaaa aacgaacatt gaattgaaac caagccaact gaaaaattcc ttatttaaat
     8401 gact
//
