LOCUS       mCerulean3-7aa-B        5437 bp    DNA     circular SYN 27-SEP-2021
DEFINITION  
ACCESSION   mCerulean3-7aa-B
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|submitted to Addgene*
FEATURES             Location/Qualifiers
     misc_feature    10..562
                     /gene="CMV_immearly_promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    65..352
                     /gene="CAG_enhancer"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    520..589
                     /gene="CMV promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             613..1329
                     /gene="mCerulean3"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1330..1350
                     /gene="7aa Linker"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1351..2070
                     /gene="Bcl-2"
                     /translation="MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAP
                     APGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGD
                     DFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVICVE
                     SVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLK
                     TLLSLALVGACITLGAYLSHK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    complement(2485..2791)
                     /gene="f1 ori"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2870..2898
                     /gene="AmpR Promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2982..3250
                     /gene="SV40 Promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3149..3226
                     /gene="SV40 ori"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3333..4124
                     /gene="NeoR/KanR"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    4302..4571
                     /gene="TK_PA_terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4719..5338
                     /gene="pB322_ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgagc tggggcgtgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaacgcc atccacggca acgtctatat caccgccgac
     1081 aagcagaaga acggcatcaa ggccaacttc ggcctcaact gcaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atccacgcgt atggcgcacg ctgggagaac agggtacgat
     1381 aaccgggaga tagtgatgaa gtacatccat tataagctgt cgcagagggg ctacgagtgg
     1441 gatgcgggag atgtgggcgc cgcgcccccg ggggccgccc ccgcaccggg catcttctcc
     1501 tcccagcccg ggcacacgcc ccatccagcc gcatcccggg acccggtcgc caggacctcg
     1561 ccactgcaga ccccggctgc ccccggcgcc gccgcggggc ctgcgctcag cccggtgcca
     1621 cctgtggtcc acctgaccct ccgccaggcc ggcgacgact tctcccgccg ctaccgccgc
     1681 gacttcgccg agatgtccag ccagctgcac ctgacgccct tcaccgcgcg gggacgcttt
     1741 gccacggtgg tggaggagct cttcagggac ggggtgaact gggggaggat tgtggccttc
     1801 tttgagttcg gtggggtcat atgtgtggag agcgtcaacc gggagatgtc gcccctggtg
     1861 gacaacatcg ccctgtggat gactgagtac ctgaaccggc acctgcacac ctggatccag
     1921 gataacggag gctgggatgc ctttgtggag ctctacggcc ccagcatgcg gcctctgttt
     1981 gatttctcct ggctgtctct taagactctg ctctcgctag cactagtggg agcatgcatc
     2041 accctgggtg cctatctgtc ccacaagtga ctcggtcgac ggtaccgcgg gcccgggatc
     2101 caccggatct agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc
     2161 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     2221 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2341 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2401 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     2461 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     2521 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     2581 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     2641 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     2701 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     2761 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     2821 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2881 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     2941 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3001 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     3061 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3121 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3181 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3241 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     3301 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     3361 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3421 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3481 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     3541 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3601 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3661 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3721 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     3781 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     3841 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3901 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3961 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4021 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4081 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     4141 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     4201 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4261 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     4321 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     4381 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     4441 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     4501 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     4561 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     4621 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4681 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4741 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4801 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4861 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4921 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4981 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5041 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5101 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5161 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5221 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5281 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5341 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5401 ccctgattct gtggataacc gtattaccgc catgcat
//
