LOCUS       Venus-30aa-Noxa-        4914 bp    DNA     circular SYN 11-FEB-2021
DEFINITION  
ACCESSION   Venus-30aa-Noxa-
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    10..562
                     /gene="CMV_immearly_promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    65..352
                     /gene="CAG_enhancer"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    520..589
                     /gene="CMV promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             613..1329
                     /gene="Venus"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKN
                     GIKVNFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLERVTAAGITHGMDELYK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1330..1419
                     /gene="30aa linker"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1420..1584
                     /gene="Noxa-4E"
                     /translation="MPGKKARKNAQPSPARAPAELEVEEATQERREGDKENFRQKLLN
                     LISKLFCSGT"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_signal     1474..1533
                     /label=BH3
                     /SECDrawAs="Label"
     misc_feature    1587..1597
                     /gene="StopCodons"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1595..1823
                     /gene="SV40_PA_terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    complement(1962..2268)
                     /gene="f1 ori"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2347..2375
                     /gene="AmpR Promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2459..2727
                     /gene="SV40 Promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2626..2703
                     /gene="SV40 ori"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             2810..3601
                     /gene="NeoR/KanR"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    3779..4048
                     /gene="TK_PA_terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4196..4815
                     /gene="pB322_ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     1081 aagcagaaga acggcatcaa ggtcaacttc aagatccgcc acaacatcga ggacggcggc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga acgcgtgacc gccgccggga tcactcacgg catggacgag
     1321 ctgtacaagt ccggactcac tagtggcgcc ctcgagacgc gtcccggggg taccgctgga
     1381 ccggtcgccg gcgagacgcg tggcagatct cgaggaggca tgcctgggaa gaaggcgcgc
     1441 aagaacgctc aaccgagccc cgcgcgggct ccagcagagc tggaagtcga ggaagctact
     1501 caagagagga gagaaggaga caaagagaac ttccggcaga aacttctgaa tctgatatcc
     1561 aaactcttct gctcaggaac ctgatctaga taactgatca taatcagcca taccacattt
     1621 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1681 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     1741 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1801 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt
     1861 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     1921 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     1981 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     2041 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     2101 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     2161 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     2221 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     2281 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     2341 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2401 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     2461 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     2521 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2581 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     2641 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     2701 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     2761 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     2821 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     2881 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     2941 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     3001 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     3061 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     3121 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     3181 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     3241 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     3301 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     3361 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     3421 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     3481 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     3541 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     3601 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     3661 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     3721 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     3781 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     3841 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     3901 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     3961 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     4021 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     4081 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4141 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4201 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4261 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4321 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     4381 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4441 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4501 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4561 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4621 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4681 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4741 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4801 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4861 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
