LOCUS       Venus-Bik-GG153A        5225 bp    DNA     circular SYN 26-SEP-2021
DEFINITION  
ACCESSION   Venus-Bik-GG153A
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    10..562
                     /gene="CMV_immearly_promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    65..352
                     /gene="CAG_enhancer"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    520..589
                     /gene="CMV promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             613..1329
                     /gene="Venus"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1330..1353
                     /gene="8aa Linker"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1354..1836
                     /gene="Bik"
                     /translation="MSEVRPLSRDILMETLLYEQLLEPPTMEVLGMTDSEEDLDPMED
                     FDSLECMEGSDALALRLACIGDEMDVSLRAPRLAQLSEVAMHSLGLAFIYDQTEDIRD
                     VLRSFMDGFTTLKENIMRFWRSPNPGSWVSCEQVLLALLLLLALLLPLLSAALHLLLK
                     "
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_signal     1504..1563
                     /label=BH3
                     /SECDrawAs="Label"
     misc_signal     1811..1815
                     /label=GG153AA
                     /SECDrawAs="Label"
     misc_feature    1837..1889
                     /gene="MCS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1898..1908
                     /gene="StopCodons"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1906..2134
                     /gene="SV40_PA_terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    complement(2273..2579)
                     /gene="f1 ori"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2658..2686
                     /gene="AmpR Promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2770..3038
                     /gene="SV40 Promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2937..3014
                     /gene="SV40 ori"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3121..3912
                     /gene="NeoR/KanR"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    4090..4359
                     /gene="TK_PA_terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4507..5126
                     /gene="pB322_ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga acgcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ctggactcag atcccgagga ggcatgtctg aagtaagacc cctctccaga
     1381 gacatcttga tggagaccct cctgtatgag cagctcctgg aacccccgac catggaggtt
     1441 cttggcatga ctgactctga agaggacctg gaccctatgg aggacttcga ttctttggaa
     1501 tgcatggagg gcagtgacgc attggccctg cggctggcct gcatcgggga cgagatggac
     1561 gtgagcctca gggccccgcg cctggcccag ctctccgagg tggccatgca cagcctgggt
     1621 ctggctttca tctacgacca gactgaggac atcagggatg ttcttagaag tttcatggac
     1681 ggtttcacca cacttaagga gaacataatg aggttctgga gatccccgaa ccccgggtcc
     1741 tgggtgtcct gcgaacaggt gctgctggcg ctgctgctgc tgctggcgct gctgctgccg
     1801 ctgctcagcg ctgctctgca cctgctgctc aagtgactcg agctcaagct tcgaattctg
     1861 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
     1921 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     1981 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2041 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2101 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt
     2161 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2221 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2281 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2341 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     2401 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     2461 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     2521 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     2581 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2641 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2701 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     2761 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2821 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     2881 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     2941 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3001 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3061 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     3121 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3181 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3241 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3301 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3361 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     3421 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     3481 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     3541 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     3601 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     3661 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     3721 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     3781 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     3841 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     3901 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     3961 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     4021 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     4081 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     4141 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     4201 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     4261 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     4321 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     4381 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4441 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4501 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4561 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4621 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4681 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4741 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4801 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4861 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4921 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4981 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5041 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5101 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5161 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     5221 tgcat
//
