LOCUS       mCherry-Cb5-IRES        9682 bp    DNA     circular SYN 28-SEP-2021
DEFINITION  Ligation of SpeI-mCherry-Cb5-BamHI into BB
ACCESSION   mCherry-Cb5-IRES
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  BB
               Fragment ends: BamHI and SpeI
               Fragment size: 8805
            Insert molecule:  SpeI-mCherry-Cb5-BamHI
               Fragment ends: SpeI and BamHI
               Fragment size: 877
FEATURES             Location/Qualifiers
     misc_feature    148..196
                     /label=WPRE
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=woodchuck hepatitis virus
                     posttranscriptional regulatory element \
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="WPRE"
     misc_feature    636..653
                     /label=PBS
                     /SerialCloner_Color=&hFF80C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=primer binding site \
                     /SECDrawAs="Label"
                     /SECName="PBS"
     misc_feature    685..822
                     /label=packaging signal
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="packaging signal"
     misc_feature    745..789
                     /label=HIV-1 psi pack
                     /SerialCloner_Color=&hFFA500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     /SECDrawAs="Label"
                     /SECName="HIV-1 psi pack"
     misc_feature    1303..1536
                     /label=RRE
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Rev-response element \
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="RRE"
     misc_feature    2185..3519
                     /label=human elongation factor 1alpha
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SECDrawAs="Label"
                     /SECName="human elongation factor 1alpha"
     misc_feature    3353..3372
                     /label=seq primer
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True 3552..4975
                     /label=insert from PCR(PSS1(human).txt.xdna)
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from
                     PCR(PSS1(human).txt.xdna) [1424  nt] : (SpeI[3] /
                     BamHI[1431]) \
                     /SECDrawAs="Label"
                     /SECName="seq primerinsert from PCR(PSS1(human).txt.xdna)"
     CDS             3553..4260
                     /gene="mCherry"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             4261..4281
                     /gene="linker"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             4282..4392
                     /gene="Cb5"
                     /product="Autotranslation of CYTB5"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    4431..4994
                     /label=IRES
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="IRES"
     misc_feature    5038..5625
                     /label=Puromycin
                     /SerialCloner_Color=&hFF8040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Puromycin"
     CDS             complement(6122..6133)
                     /label=FactorXa site
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="FactorXa site"
     misc_feature    6445..7081
                     /label=3' LTR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="3' LTR"
     misc_feature    6593..6641
                     /label=WPRE
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=woodchuck hepatitis virus
                     posttranscriptional regulatory element \
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="WPRE"
     CDS             complement(8371..9030)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="AmpR"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 ctcgagacta gtatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     3601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     3661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     3721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     3781 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     3841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     3901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     4021 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     4081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     4141 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     4201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     4261 tccggactca gatctggtaa gatcactacc gttgaatcga actctagttg gtggactaac
     4321 tgggttatcc ctgcgatctc tgctctggtt gtagcgctga tgtaccgtct gtatatggct
     4381 gaagattaat gagaattctg cagtcgacgg taccgcgggc ccgggatccc gcccctctcc
     4441 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     4501 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     4561 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     4621 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     4681 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     4741 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     4801 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     4861 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     4921 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     4981 tttcctttga aaaacacgat gataagcttg ccacaaccca caaggagacg accttccatg
     5041 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc
     5101 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc
     5161 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     5221 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     5281 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     5341 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5401 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     5461 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5521 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5581 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctagacg
     5641 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5701 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5761 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5821 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5881 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5941 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6001 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     6061 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6121 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6181 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     6241 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     6301 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     6361 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6421 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     6481 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     6541 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     6601 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     6661 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     6721 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     6781 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     6841 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     6901 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6961 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7021 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7081 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     7141 gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta
     7201 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7261 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7321 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7381 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7561 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7621 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7681 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7741 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7801 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7861 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7921 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7981 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8041 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8101 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8161 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8221 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8281 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8341 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8401 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8461 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8521 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8581 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8641 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8701 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8761 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8821 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8881 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8941 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9001 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9061 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9121 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9181 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9241 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9301 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9361 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
     9421 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9481 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     9541 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     9601 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     9661 tatgtactac aaacttagta gt
//
