LOCUS       Exported                8474 bp ds-DNA     circular SYN 07-OCT-2021
DEFINITION  Ligation of Fragment 2 into Fragment 2.
ACCESSION   .
VERSION     .
KEYWORDS    pubc-mcherry-FSE-copGFP (-1 PRF)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 7, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
COMMENT     Vector molecule:  Fragment 2
               Fragment ends: ClaI and AgeI
               Fragment size: 6988
            Insert molecule:  Fragment 2
               Fragment ends: AgeI and ClaI
               Fragment size: 1355
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..636
                     /label=5'LTR
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    637..1155
                     /label=PSI
                     /note="PSI packaging sequence"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    1030..1536
                     /label=RRE
                     /note="minimal RRE"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    2034..2211
                     /label=TAR
                     /note="Central Poly Purine Tract"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    2218..2615
                     /label=UbC
                     /note="Ubiquitin C promoter"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    2631..3338
                     /label=mCherry
                     /note="color: #ff0000; direction: RIGHT"
     CDS             3339..3362
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     misc_feature    3363..3374
                     /label=ORF1a
                     /note="color: #ff9900"
     misc_feature    3368..3374
                     /label=Slippery site
                     /note="color: #3366ff"
     misc_feature    3374..3454
                     /label=ORF1b
                     /note="color: #cc99ff"
     misc_feature    3375..3380
                     /label=Spacer region
                     /note="color: #666699"
     misc_feature    3381..3405
                     /label=Three stemmed pseudoknot
                     /note="color: #00ffff"
     misc_feature    3403..3405
                     /label=TAC deletion
                     /note="color: #ff00ff"
     misc_feature    3406..3447
                     /label=Three stemmed pseudoknot
                     /note="color: #00ffff"
     misc_feature    3456..4208
                     /label=copGFP
                     /note="/vntifkey=21"
                     /note="color: #00ff00"
     misc_feature    4209..4262
                     /label=T2A
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    4263..4862
                     /label=Puro
                     /note="/vntifkey=21"
                     /note="color: #ffff00"
     misc_feature    4863..5454
                     /label=WPRE
                     /note="Woodchuck Hepatitis Virus Posttranscriptional 
                     Regulatory Element"
                     /note="color: #cc0000"
     misc_feature    5525..5760
                     /label=dU3 LTR
                     /note="Deleted U3 Long-Terminal Repeat"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    6088..6927
                     /label=AMPr
                     /note="beta-lactamase (mature form)"
                     /note="color: #cc0000; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccgccacc atggtgagca
     2641 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     2701 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     2761 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     2821 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     2881 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     2941 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     3001 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     3061 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     3121 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     3181 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     3241 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     3301 gccgccactc caccggcggc atggacgagc tgtacaagga ctacaaggat gacgacgata
     3361 agtcgttttt aaacgggttt gcggtgtaag tgcagcccgt cttacaccgt gcggcacagg
     3421 cactagtact gatgtcgtat acagggcttt tgaccgagag cgacgagagc ggcctgcccg
     3481 ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg
     3541 gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag
     3601 gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact
     3661 tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct
     3721 acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct
     3781 accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg
     3841 aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc
     3901 accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg
     3961 gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca
     4021 gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca
     4081 acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca
     4141 gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg
     4201 gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     4261 ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     4321 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     4381 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     4441 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     4501 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     4561 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     4621 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     4681 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     4741 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     4801 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     4861 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4921 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4981 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5041 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5101 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5161 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5221 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     5281 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5341 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5401 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc
     5461 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5521 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     5581 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5641 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5701 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5761 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     5821 agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc
     5881 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5941 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6001 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6061 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6121 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6181 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6241 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6301 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6361 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6421 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6481 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6541 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6601 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6661 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6721 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6781 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6841 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6901 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6961 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7021 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7741 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7801 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7861 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7921 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7981 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     8041 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     8101 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     8161 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
     8221 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8281 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     8341 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     8401 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     8461 acaaacttag tagt
//
