LOCUS       Soat2-Insert1-pTAL-Luc  5770 bp ds-DNA     circular     31-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      10..465
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      27..333
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(467..535)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Insert          794..1793
                     /label="Insert"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1029..1202
                     /label="Exon 1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1121..1123
                     /label="ATG Start"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1694..1749
                     /label="Exon 2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1764..1769
                     /label="AGGTCA Motif"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1764..1776
                     /label="DR1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1796..3448
                     /label="Firefly Luciferase"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    3480..3671
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      complement(4011..4693)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(4791..5450)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA
       61 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT
      121 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT
      181 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC
      241 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT
      301 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT
      361 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC
      421 AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTCCCAT TCGCCATTCA
      481 GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCCCA
      541 AGCTACCATG ATAAGTAAGT AATATTAAGG TACGGGAGGT ACTTGGAGCG GCCGCAATAA
      601 AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGTACTAAC
      661 ATACGCTCTC CATCAAAACA AAACGAAACA AAACAAACTA GCAAAATAGG CTGTCCCCAG
      721 TGCAAGTGCA GGTGCCAGAA CATTTCTCTA TCGATAGGTA CCGAGCTCTT ACGCGTGCTA
      781 GCCCGGGCTC GAGCCCAAGA TGTACCCAGC CACTATTCCT GTGTGTGTGT GGCGGGGTGG
      841 ATAGCACCCT TGGAGCTGGG CATCTGGATG GTGGTTGGGG TGTTAGGGGG ATGTGGCGAT
      901 CCCTGCGACA GACAGCAGAG TGAGGTCTGA ATCTGAGAAG CTCTGCCTCC AGATCAGATA
      961 ACCTATCGCA CTCCCAGAGG CCCCCTCCTC CTGAAAGGAC TTTAGTCTTT GGAGCTGTCA
     1021 CCTGAGCTGA GTGGGACAAG AGCTCTACAG GGCAGGCCAC ACTGCGAAGG AAGGAGGCAA
     1081 CACGGGCAAG GGCTGCCTGC TGCCCGCTGG AGACCGCACC ATGGAGCCAG GCGGGGCCCG
     1141 TCTGCGTCTG CAGAGGACAG AAGGGCTGGG AGGGGAGCGG GAGCGCCAAC CCTGTGGAGA
     1201 TGGTGAGCCG CCCTCGGGGG TGCAGAAGGC ACAGGCAAGT GGGGGGGAGG CGGGGAGAGA
     1261 TGCGCTATGG AGAGAAGGCT CCAACTGCCT GATGCCAATC CTTCCTGCTG TCCAGAGAGG
     1321 CCAAGGAAGG TAGACCCTGG CCTCAGCCCA GAGCCACCCA GCTGGAAGAT GGAACAGAAA
     1381 AGAGCTATGT CAGAGCTGTG GCCTGGCCTT ATCCTTCTGA CTGTTTAGCC CCAGATAGTG
     1441 AATGGAGTAG CTTCCCATTC CCTGAGTGCC CTGTTCCCAG TAATGTCACC AGGCCCCCTG
     1501 CTTGCCTATG GCCTCCTCAC CACTGGCAAC AGCAACTTCC CCTTCTAGTA GCCCCAACCA
     1561 TGATACTAGA TATTGGCTGG TTGGGGTGAG GGCAGCTGCT GAGCACACAA AGCATTTCCT
     1621 GACCACAGGA TGCCTCTGGG TCTGCAGAAC CCCAATTCCT CCTGTTGACT GTGCCTTTGA
     1681 TCCCTCCTCA CAGGAAACAC TGAGACGCAC AGAGCCCCGG ACTTGGTACA ATGGACCCGA
     1741 CACATGGAGG TGAGGGATGT CAGAGGTCAG AGGTCAAGTG GACAGATCCT TGGCCATGGA
     1801 AGACGCCAAA AACATAAAGA AAGGCCCGGC GCCATTCTAT CCGCTGGAAG ATGGAACCGC
     1861 TGGAGAGCAA CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT
     1921 TACAGATGCA CATATCGAGG TGGACATCAC TTACGCTGAG TACTTCGAAA TGTCCGTTCG
     1981 GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT CACAGAATCG TCGTATGCAG
     2041 TGAAAACTCT CTTCAATTCT TTATGCCGGT GTTGGGCGCG TTATTTATCG GAGTTGCAGT
     2101 TGCGCCCGCG AACGACATTT ATAATGAACG TGAATTGCTC AACAGTATGG GCATTTCGCA
     2161 GCCTACCGTG GTGTTCGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA
     2221 GCTCCCAATC ATCCAAAAAA TTATTATCAT GGATTCTAAA ACGGATTACC AGGGATTTCA
     2281 GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT TTTAATGAAT ACGATTTTGT
     2341 GCCAGAGTCC TTCGATAGGG ACAAGACAAT TGCACTGATC ATGAACTCCT CTGGATCTAC
     2401 TGGTCTGCCT AAAGGTGTCG CTCTGCCTCA TAGAACTGCC TGCGTGAGAT TCTCGCATGC
     2461 CAGAGATCCT ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC
     2521 ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG GATTTCGAGT
     2581 CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTCTGAGG AGCCTTCAGG ATTACAAGAT
     2641 TCAAAGTGCG CTGCTGGTGC CAACCCTATT CTCCTTCTTC GCCAAAAGCA CTCTGATTGA
     2701 CAAATACGAT TTATCTAATT TACACGAAAT TGCTTCTGGT GGCGCTCCCC TCTCTAAGGA
     2761 AGTCGGGGAA GCGGTTGCCA AGAGGTTCCA TCTGCCAGGT ATCAGGCAAG GATATGGGCT
     2821 CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC CGGGCGCGGT
     2881 CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT CTGGATACCG GGAAAACGCT
     2941 GGGCGTTAAT CAAAGAGGCG AACTGTGTGT GAGAGGTCCT ATGATTATGT CCGGTTATGT
     3001 AAACAATCCG GAAGCGACCA ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA
     3061 CATAGCTTAC TGGGACGAAG ACGAACACTT CTTCATCGTT GACCGCCTGA AGTCTCTGAT
     3121 TAAGTACAAA GGCTATCAGG TGGCTCCCGC TGAATTGGAA TCCATCTTGC TCCAACACCC
     3181 CAACATCTTC GACGCAGGTG TCGCAGGTCT TCCCGACGAT GACGCCGGTG AACTTCCCGC
     3241 CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC GATGACGGAA AAAGAGATCG TGGATTACGT
     3301 CGCCAGTCAA GTAACAACCG CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT
     3361 ACCGAAAGGT CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC
     3421 CAAGAAGGGC GGAAAGATCG CCGTGTAATT CTAGAGTCGG GGCGGCCGGC CGCTTCGAGC
     3481 AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA
     3541 ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA
     3601 TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG
     3661 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT AAAATCGATA AGGATCCGTC
     3721 GACCGATGCC CTTGAGAGCC TTCAACCCAG TCAGCTCCTT CCGGTGGGCG CGGGGCATGA
     3781 CTATCGTCGC CGCACTTATG ACTGTCTTCT TTATCATGCA ACTCGTAGGA CAGGTGCCGG
     3841 CAGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA
     3901 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA
     3961 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
     4021 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
     4081 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
     4141 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
     4201 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
     4261 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
     4321 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
     4381 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
     4441 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG
     4501 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
     4561 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
     4621 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     4681 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     4741 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     4801 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     4861 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     4921 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     4981 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     5041 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     5101 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     5161 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     5221 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     5281 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     5341 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     5401 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     5461 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     5521 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     5581 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     5641 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     5701 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT
     5761 CCCCGAAAAG
//