LOCUS       pSIBR005                6937 bp ds-DNA     circular     06-OCT-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    49..70
                     /label="B1002 terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        77..106
                     /label="lacUV5 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    112..147
                     /label="Repeat"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    148..186
                     /label="NT spacer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    187..222
                     /label="Repeat"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      243..264
                     /label="B1002 terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    372..1187
                     /label="Kan Neo"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1261..1920)
                     /label="'rep"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1921..2692
                     /label="Ori pBBR1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2937..2966
                     /label="lacUV5 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3035..6937
                     /label="Cas12a "
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(3717..3747)
                     /label="primer for cas12 ecoppu no intron RV"
                     /note="sequence: CAAAGGTTAGCTCTTCTGCCAAATCTTTTTTAATTTGTTCATAGTTTATAG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 GCGGCCGCAT AATGATGTGT TATCATTGAT GCGAGGTCGC CTATACCTCC CCGCTTCGGC
       61 GGGGTTTTTT CCCGGGTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT GGTCTAAGAA
      121 CTTTAAATAA TTTCTACTGT TGTAGATATG TCTTCCTGAG TGACGAGCAA CGAGACTGGA
      181 AGACATGTCT AAGAACTTTA AATAATTTCT ACTGTTGTAG ATAGATACCG GACAACGTGT
      241 CTCCCCGCTT CGGCGGGGTT TTTTCCTAGG TTGTGTCTCA AAATCTCTGA TGTTACATTG
      301 CACAAGATAA AAATATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA
      361 CAAGGGGTGT TATGAGCCAT ATTCAGCGTG AAACGAGCTG TAGCCGTCCG CGTCTGAACA
      421 GCAACATGGA TGCGGATCTG TATGGCTATA AATGGGCGCG TGATAACGTG GGTCAGAGCG
      481 GCGCGACCAT TTATCGTCTG TATGGCAAAC CGGATGCGCC GGAACTGTTT CTGAAACATG
      541 GCAAAGGCAG CGTGGCGAAC GATGTGACCG ATGAAATGGT GCGTCTGAAC TGGCTGACCG
      601 AATTTATGCC GCTGCCGACC ATTAAACATT TTATTCGCAC CCCGGATGAT GCGTGGCTGC
      661 TGACCACCGC GATTCCGGGC AAAACCGCGT TTCAGGTGCT GGAAGAATAT CCGGATAGCG
      721 GCGAAAACAT TGTGGATGCG CTGGCCGTGT TTCTGCGTCG TCTGCATAGC ATTCCGGTGT
      781 GCAACTGCCC GTTTAACAGC GATCGTGTGT TTCGTCTGGC CCAGGCGCAG AGCCGTATGA
      841 ACAACGGCCT GGTGGATGCG AGCGATTTTG ATGATGAACG TAACGGCTGG CCGGTGGAAC
      901 AGGTGTGGAA AGAAATGCAT AAACTGCTGC CGTTTAGCCC GGATAGCGTG GTGACCCACG
      961 GCGATTTTAG CCTGGATAAC CTGATTTTCG ATGAAGGCAA ACTGATTGGC TGCATTGATG
     1021 TGGGCCGTGT GGGCATTGCG GATCGTTATC AGGATCTGGC CATTCTGTGG AACTGCCTGG
     1081 GCGAATTTAG CCCGAGCCTG CAAAAACGTC TGTTTCAGAA ATATGGCATT GATAATCCGG
     1141 ATATGAACAA ACTGCAATTT CATCTGATGC TGGATGAATT TTTCTAATAA TTAATTGGAC
     1201 CGCGGTCCGA GAGACGCATG GCAGTATCTC AGACTGTGTA TCGGCACGTC TCACTACTTA
     1261 CCGGCGCGGC AGCGTTACCC GTGTCGGCGG CTCCAACGGC TCGCCATCGT CCAGAAAACA
     1321 CGGCTCATCG GGCATCGGCA GGCGCTGCTG CCCGCGCCGT TCCCATTCCT CCGTTTCGGT
     1381 CAAGGCTGGC AGGTCTGGTT CCATGCCCGG AATGCCGGGC TGGCTGGGCG GCTCCTCGCC
     1441 GGGGCCGGTC GGTAGTTGCT GCTCGCCCGG ATACAGGGTC GGGATGCGGC GCAGGTCGCC
     1501 ATGCCCCAAC AGCGATTCGT CCTGGTCGTC GTGATCAACC ACCACGGCGG CACTGAACAC
     1561 CGACAGGCGC AACTGGTCGC GGGGCTGGCC CCACGCCACG CGGTCATTGA CCACGTAGGC
     1621 CGACACGGTG CCGGGGCCGT TGAGCTTCAC GACGGAGATC CAGCGCTCGG CCACCAAGTC
     1681 CTTGACTGCG TATTGGACCG TCCGCAAAGA ACGTCCGATG AGCTTGGAAA GTGTTTTCTG
     1741 GCTGACCACC ACGGCGTTCT GGTGGCCCAT CTGCGCCACG AGGTGATGCA GCAGCATTGC
     1801 CGCCGTGGGT TTCCTCGCAA TAAGCCCGGC CCACGCCTCA TGCGCTTTGC GTTCCGTTTG
     1861 CACCCAGTGA CCGGGCTTGT TCTTGGCTTG AATGCCGATT TCTCTGGACT GCGTGGCCAT
     1921 GCTTATCTCC ATGCGGTAGG GGTGCCGCAC GGTTGCGGCA CCATGCGCAA TCAGCTGCAA
     1981 CTTTTCGGCA GCGCGACAAC AATTATGCGT TGCGTAAAAG TGGCAGTCAA TTACAGATTT
     2041 TCTTTAACCT ACGCAATGAG CTATTGCGGG GGGTGCCGCA ATGAGCTGTT GCGTACCCCC
     2101 CTTTTTTAAG TTGTTGATTT TTAAGTCTTT CGCATTTCGC CCTATATCTA GTTCTTTGGT
     2161 GCCCAAAGAA GGGCACCCCT GCGGGGTTCC CCCACGCCTT CGGCGCGGCT CCCCCTCCGG
     2221 CAAAAAGTGG CCCCTCCGGG GCTTGTTGAT CGACTGCGCG GCCTTCGGCC TTGCCCAAGG
     2281 TGGCGCTGCC CCCTTGGAAC CCCCGCACTC GCCGCCGTGA GGCTCGGGGG GCAGGCGGGC
     2341 GGGCTTCGCC CTTCGACTGC CCCCACTCGC ATAGGCTTGG GTCGTTCCAG GCGCGTCAAG
     2401 GCCAAGCCGC TGCGCGGTCG CTGCGCGAGC CTTGACCCGC CTTCCACTTG GTGTCCAACC
     2461 GGCAAGCGAA GCGCGCAGGC CGCAGGCCGG AGGCTTTTCC CCAGAGAAAA TTAAAAAAAT
     2521 TGATGGGGCA AGGCCGCAGG CCGCGCAGTT GGAGCCGGTG GGTATGTGGT CGAAGGCTGG
     2581 GTAGCCGGTG GGCAATCCCT GTGGTCAAGC TCGTGGGCAG GCGCAGCCTG TCCATCAGCT
     2641 TGTCCAGCAG GGTTGTCCAC GGGCCGAGCG AAGCGAGCCA GCCGGTGGCC GCTCGCGGCC
     2701 ATCGTCCACA TATCCACGGG CTGGCAAGGG AGCGCAGCGA CCGCGCAGGG CGAAGCCCGG
     2761 AGAGCAAGCC CGTAGGGGGG GCGCGCCCAG CTGTCTAGGG CGGCGGATTT GTCCTACTCA
     2821 GGAGAGCGTT CACCGACAAA CAACAGATAA AACGAAAGGC CCAGTCTTTC GACTGAGCCT
     2881 TTCGTTTTAT TTGATGCCTT TAATTAAAGC GGATAACAAT TTCACACAGG AGGCCGTTTA
     2941 CACTTTATGC TTCCGGCTCG TATAATGTGT GGGGAAACCA CAACGGTTTC CCTCTAGAAA
     3001 TAATTTTGTT TAACTATAAG AAGGAGATAT ACATATGTCA ATTTATCAAG AATTTGTTAA
     3061 TAAATATAGT TTAAGTAAAA CTCTAAGATT TGAGTTAATC CCACAGGGTA AAACACTTGA
     3121 AAACATAAAA GCAAGAGGTT TGATTTTAGA TGATGAGAAA AGAGCTAAAG ACTACAAAAA
     3181 GGCTAAACAA ATAATTGATA AATATCATCA GTTTTTTATA GAGGAGATAT TAAGTTCGGT
     3241 TTGTATTAGC GAAGATTTAT TACAAAACTA TTCTGATGTT TATTTTAAAC TTAAAAAGAG
     3301 TGATGATGAT AATCTACAAA AAGATTTTAA AAGTGCAAAA GATACGATAA AGAAACAAAT
     3361 ATCTGAATAT ATAAAGGACT CAGAGAAATT TAAGAATTTG TTTAATCAAA ACCTTATCGA
     3421 TGCTAAAAAA GGGCAAGAGT CAGATTTAAT TCTATGGCTA AAGCAATCTA AGGATAATGG
     3481 TATAGAACTA TTTAAAGCCA ATAGTGATAT CACAGATATA GATGAGGCGT TAGAAATAAT
     3541 CAAATCTTTT AAAGGTTGGA CAACTTATTT TAAGGGTTTT CATGAAAATA GAAAAAATGT
     3601 TTATAGTAGC AATGATATTC CTACATCTAT TATTTATAGG ATAGTAGATG ATAATTTGCC
     3661 TAAATTTCTA GAAAATAAAG CTAAGTATGA GAGTTTAAAA GACAAAGCTC CAGAAGCTAT
     3721 AAACTATGAA CAAATTAAAA AAGATTTGGC AGAAGAGCTA ACCTTTGATA TTGACTACAA
     3781 AACATCTGAA GTTAATCAAA GAGTTTTTTC ACTTGATGAA GTTTTTGAGA TAGCAAACTT
     3841 TAATAATTAT CTAAATCAAA GTGGTATTAC TAAATTTAAT ACTATTATTG GTGGTAAATT
     3901 TGTAAATGGT GAAAATACAA AGAGAAAAGG TATAAATGAA TATATAAATC TATACTCACA
     3961 GCAAATAAAT GATAAAACAC TCAAAAAATA TAAAATGAGT GTTTTATTTA AGCAAATTTT
     4021 AAGTGATACA GAATCTAAAT CTTTTGTAAT TGATAAGTTA GAAGATGATA GTGATGTAGT
     4081 TACAACGATG CAAAGTTTTT ATGAGCAAAT AGCAGCTTTT AAAACAGTAG AAGAAAAATC
     4141 TATTAAAGAA ACACTATCTT TATTATTTGA TGATTTAAAA GCTCAAAAAC TTGATTTGAG
     4201 TAAAATTTAT TTTAAAAATG ATAAATCTCT TACTGATCTA TCACAACAAG TTTTTGATGA
     4261 TTATAGTGTT ATTGGTACAG CGGTACTAGA ATATATAACT CAACAAATAG CACCTAAAAA
     4321 TCTTGATAAC CCTAGTAAGA AAGAGCAAGA ATTAATAGCC AAAAAAACTG AAAAAGCAAA
     4381 ATACTTATCT CTAGAAACTA TAAAGCTTGC CTTAGAAGAA TTTAATAAGC ATAGAGATAT
     4441 AGATAAACAG TGTAGGTTTG AAGAAATACT TGCAAACTTT GCGGCTATTC CGATGATATT
     4501 TGATGAAATA GCTCAAAACA AAGACAATTT GGCACAGATA TCTATCAAAT ATCAAAATCA
     4561 AGGTAAAAAA GACCTACTTC AAGCTAGTGC GGAAGATGAT GTTAAAGCTA TCAAGGATCT
     4621 TTTAGATCAA ACTAATAATC TCTTACATAA ACTAAAAATA TTTCATATTA GTCAGTCAGA
     4681 AGATAAGGCA AATATTTTAG ACAAGGATGA GCATTTTTAT CTAGTATTTG AGGAGTGCTA
     4741 CTTTGAGCTA GCGAATATAG TGCCTCTTTA TAACAAAATT AGAAACTATA TAACTCAAAA
     4801 GCCATATAGT GATGAGAAAT TTAAGCTCAA TTTTGAGAAC TCGACTTTGG CTAATGGTTG
     4861 GGATAAAAAT AAAGAGCCTG ACAATACGGC AATTTTATTT ATCAAAGATG ATAAATATTA
     4921 TCTGGGTGTG ATGAATAAGA AAAATAACAA AATATTTGAT GATAAAGCTA TCAAAGAAAA
     4981 TAAAGGCGAG GGTTATAAAA AAATTGTTTA TAAACTTTTA CCTGGCGCAA ATAAAATGTT
     5041 ACCTAAGGTT TTCTTTTCTG CTAAATCTAT AAAATTTTAT AATCCTAGTG AAGATATACT
     5101 TAGAATAAGA AATCATTCCA CACATACAAA AAATGGTAGT CCTCAAAAAG GATATGAAAA
     5161 ATTTGAGTTT AATATTGAAG ATTGCCGAAA ATTTATAGAT TTTTATAAAC AGTCTATAAG
     5221 TAAGCATCCG GAGTGGAAAG ATTTTGGATT TAGATTTTCT GATACTCAAA GATATAATTC
     5281 TATAGATGAA TTTTATAGAG AAGTTGAAAA TCAAGGCTAC AAACTAACTT TTGAAAATAT
     5341 ATCAGAGAGC TATATTGATA GCGTAGTTAA TCAGGGTAAA TTGTACCTAT TCCAAATCTA
     5401 TAATAAAGAT TTTTCAGCTT ATAGCAAAGG GCGACCAAAT CTACATACTT TATATTGGAA
     5461 AGCGCTGTTT GATGAGAGAA ATCTTCAAGA TGTGGTTTAT AAGCTAAATG GTGAGGCAGA
     5521 GCTTTTTTAT CGTAAACAAT CAATACCTAA AAAAATCACT CACCCAGCTA AAGAGGCAAT
     5581 AGCTAATAAA AACAAAGATA ATCCTAAAAA AGAGAGTGTT TTTGAATATG ATTTAATCAA
     5641 AGATAAACGC TTTACTGAAG ATAAGTTTTT CTTTCACTGT CCTATTACAA TCAATTTTAA
     5701 ATCTAGTGGA GCTAATAAGT TTAATGATGA AATCAATTTA TTGCTAAAAG AAAAAGCAAA
     5761 TGATGTTCAT ATATTAAGTA TAGATAGAGG TGAAAGACAT TTAGCTTACT ATACTTTGGT
     5821 AGATGGTAAA GGCAATATCA TCAAACAAGA TACTTTCAAC ATCATTGGTA ATGATAGAAT
     5881 GAAAACAAAC TACCATGATA AGCTTGCTGC AATAGAGAAA GATAGGGATT CAGCTAGGAA
     5941 AGACTGGAAA AAGATAAATA ACATCAAAGA GATGAAAGAG GGCTATCTAT CTCAGGTAGT
     6001 TCATGAAATA GCTAAGCTAG TTATAGAGTA TAATGCTATT GTGGTTTTTG AGGATTTAAA
     6061 TTTTGGATTT AAAAGAGGGC GTTTCAAGGT AGAGAAGCAG GTCTATCAAA AGTTAGAAAA
     6121 AATGCTAATT GAGAAACTAA ACTATCTAGT TTTCAAAGAT AATGAGTTTG ATAAAACTGG
     6181 GGGAGTGCTT AGAGCTTATC AGCTAACAGC ACCTTTTGAG ACTTTTAAAA AGATGGGTAA
     6241 ACAAACAGGT ATTATCTACT ATGTACCAGC TGGTTTTACT TCAAAAATTT GTCCTGTAAC
     6301 TGGTTTTGTA AATCAGTTAT ATCCTAAGTA TGAAAGTGTC AGCAAATCTC AAGAGTTCTT
     6361 TAGTAAGTTT GACAAGATTT GTTATAACCT TGATAAGGGC TATTTTGAGT TTAGTTTTGA
     6421 TTATAAAAAC TTTGGTGACA AGGCTGCCAA AGGCAAGTGG ACTATAGCTA GCTTTGGGAG
     6481 TAGATTGATT AACTTTAGAA ATTCAGATAA AAATCATAAT TGGGATACTC GAGAAGTTTA
     6541 TCCAACTAAA GAGTTGGAGA AATTGCTAAA AGATTATTCT ATCGAATATG GGCATGGCGA
     6601 ATGTATCAAA GCAGCTATTT GCGGTGAGAG CGACAAAAAG TTTTTTGCTA AGCTAACTAG
     6661 TGTCCTAAAT ACTATCTTAC AAATGCGTAA CTCAAAAACA GGTACTGAGT TAGATTATCT
     6721 AATTTCACCA GTAGCAGATG TAAATGGCAA TTTCTTTGAT TCGCGACAGG CGCCAAAAAA
     6781 TATGCCTCAA GATGCTGATG CCAATGGTGC TTATCATATT GGGCTAAAAG GTCTGATGCT
     6841 ACTAGGTAGG ATCAAAAATA ATCAAGAGGG CAAAAAACTC AATTTGGTTA TCAAAAATGA
     6901 AGAGTATTTT GAGTTCGTGC AGAATAGGAA TAACTAA
//