LOCUS       pAAV-nEF-CoffFon-DREADD\Gi-mCherry-WPRE        7487 bp    DNA     circular      6-OCT-2021
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-14075299|
COMMENT     VNTDBDATE|-14075299|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-nEF-CoffFon-DREADD Gi-mCherry-WPRE|
COMMENT     VNTAUTHORNAME|Charu|
COMMENT     VNTAUTHOREML|charur@stanford.edu|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV-nEF-CoffFon-DREADD Gi-mCherry-WPRE" 0 7487 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AgeI" "AscI" "BamHI" "BsrGI" "EcoRI" "KpnI" "NheI" "SalI" 
COMMENT         "SpeI" "AarI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" "AclI" "AcuI" 
COMMENT         "AfeI" "AflII" "AflIII" "AhdI" "AleI" "AloI" "AluI" "AlwI" "AlwNI" 
COMMENT         "ApaBI" "ApaI" "ApaLI" "ApoI" "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" 
COMMENT         "BaeI" "BanI" "BanII" "BbeI" "Bbr7I" "BbsI" "BbvCI" "BbvI" "BccI" 
COMMENT         "BceAI" "BcefI" "BcgI" "BciVI" "BclI" "BfaI" "BfrBI" "BglI" "BglII" 
COMMENT         "BlpI" "Bme1580I" "BmgBI" "BmrI" "BmtI" "BplI" "BpmI" "Bpu10I" "BpuEI" 
COMMENT         "BsaAI" "BsaBI" "BsaHI" "BsaI" "BsaJI" "BsaWI" "BsaXI" "BscAI" "BseMII" 
COMMENT         "BseRI" "BseYI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI" "BslI" "BsmAI" "BsmBI" 
COMMENT         "BsmFI" "BsmI" "Bsp1286I" "Bsp24I" "BspCNI" "BspEI" "BspHI" "BspMI" 
COMMENT         "BsrBI" "BsrDI" "BsrFI" "BsrI" "BssHII" "BssSI" "BstAPI" "BstBI" 
COMMENT         "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" "BstXI" "BstYI" "BstZ17I" 
COMMENT         "Bsu36I" "BtgI" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "CjeI" "CjePI" 
COMMENT         "ClaI" "Csp6I" "CviAII" "CviJI" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" 
COMMENT         "EaeI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" "EcoNI" 
COMMENT         "EcoO109I" "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" "Fnu4HI" 
COMMENT         "FokI" "FseI" "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" "HaeIV" 
COMMENT         "HgaI" "HhaI" "Hin4I" "HincII" "HindIII" "HinfI" "HinP1I" "HpaI" 
COMMENT         "HpaII" "HphI" "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4III" "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "LpnI" "MaeIII" 
COMMENT         "MboI" "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NcoI" "NdeI" "NgoMIV" "NlaIII" 
COMMENT         "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" "PciI" "PflMI" 
COMMENT         "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" "PshAI" "PsiI" 
COMMENT         "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PstI" "PvuI" "PvuII" "RleAI" 
COMMENT         "RsaI" "RsrII" "SacI" "SacII" "SanDI" "SapI" "Sau96I" "SbfI" "ScaI" 
COMMENT         "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" "SfoI" "SgrAI" 
COMMENT         "SimI" "SmaI" "SmlI" "SnaBI" "SphI" "SrfI" "Sse232I" "Sse8647I" 
COMMENT         "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" "StyI" "SwaI" "TaiI" 
COMMENT         "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" "Tsp45I" "Tsp509I" "TspDTI" 
COMMENT         "TspGWI" "TspRI" "Tth111I" "Tth111II" "UnbI" "UthSI" "VpaK11AI" "XbaI" 
COMMENT         "XcmI" "XhoI" "XmaI" "XmnI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 7487 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 1768 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 828 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 703 0 1774 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 3 843 0 2573 0 3184 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3591 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 2733 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 2853 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 1275 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 1399 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AarI" "cacctgc" 12 16 1 1808 1 0 "") 
COMMENT        #10=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 4068 0 5676 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 2729 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AccI" "gtmkac" 3 0 3 1276 0 1379 0 2835 0 0 
COMMENT             "") 
COMMENT        #13=(CRSite (CStringList) "AceIII" "cagctc" 14 18 10 859 0 1945 0 2292 0 
COMMENT             2314 1 2430 0 3417 1 3891 0 5352 1 5549 1 6291 1 0 "") 
COMMENT        #14=(CRSite (CStringList) "AciI" "ccgc" 2 4 84 38 0 143 1 147 0 270 1 
COMMENT             307 0 387 0 439 0 442 0 457 0 488 0 495 0 522 0 860 0 874 1 1022 0 
COMMENT             1087 0 1157 0 1438 0 1516 1 1615 1 1638 1 1779 0 2042 0 2052 0 2094 
COMMENT             1 2108 0 2226 0 2228 1 2245 1 2254 1 2366 1 2372 1 2387 1 2431 1 
COMMENT             2444 1 2927 0 3071 1 3924 1 3937 0 3950 0 4060 1 4101 1 4115 0 4117 
COMMENT             1 4136 1 4146 0 4192 0 4200 0 4428 1 4488 0 4734 1 4743 1 4747 0 
COMMENT             4852 1 4902 1 4928 1 4941 0 4978 1 4992 1 4995 1 5023 0 5050 0 5455 
COMMENT             0 5481 0 5491 0 5530 0 5704 1 5751 0 5850 1 5959 1 6036 1 6080 0 
COMMENT             6201 1 6247 0 6438 1 6529 1 6891 0 6900 1 7035 0 7145 1 7266 1 7285 
COMMENT             1 7412 1 7440 1 0 "") 
COMMENT        #15=(CRSite (CStringList) "AclI" "aacgtt" 3 0 4 363 0 5409 0 5993 0 6366 
COMMENT             0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 8 422 0 1015 1 1021 1 
COMMENT             1045 1 1590 1 3264 0 5929 0 6941 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AfeI" "agcgct" 4 0 2 2382 0 4216 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AflIII" "acrygt" 2 0 2 151 0 7483 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 5 71 0 1566 0 4348 0 
COMMENT             4822 0 6595 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 2 905 0 4292 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AloI" "gaacnnnnnntcc" 26 21 4 695 1 1204 0 
COMMENT             4710 0 5235 1 0 "") 
COMMENT        #23=(CRSite (CStringList) "AluI" "agct" 3 0 41 12 0 400 0 406 0 544 0 
COMMENT             685 0 754 0 765 0 849 0 969 0 1035 0 1164 0 1326 0 1337 0 1493 0 
COMMENT             1610 0 1935 0 2282 0 2328 0 2405 0 2420 0 2631 0 3080 0 3274 0 3431 
COMMENT             0 3469 0 3605 0 3881 0 4449 0 4566 0 4880 0 5109 0 5366 0 5544 0 
COMMENT             5563 0 6242 0 6305 0 6405 0 6926 0 7183 0 7229 0 7319 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "AlwI" "ggatc" 10 11 16 167 0 672 1 698 1 711 
COMMENT             0 1769 1 1782 0 2200 0 4185 0 5956 0 5960 1 6277 0 6740 1 6741 0 
COMMENT             6837 1 6839 0 6925 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 3 1044 0 2896 0 7074 0 
COMMENT             0 "") 
COMMENT        #26=(CRSite (CStringList) "ApaBI" "gcannnnntgc" 9 0 1 1821 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 2226 0 2522 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 5 1695 0 3824 0 5426 0 
COMMENT             5923 0 7169 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "ApoI" "raatty" 2 0 4 3591 0 3634 0 5379 0 
COMMENT             5390 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "AseI" "attaat" 3 0 2 2695 0 6420 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 1 169 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 16 41 0 52 0 215 0 317 0 
COMMENT             1075 0 1479 0 2036 0 2426 0 2439 0 2523 0 3426 0 4221 0 4526 0 4836 
COMMENT             0 4847 0 5303 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 7 1911 0 1958 0 2199 0 
COMMENT             4103 0 4736 0 6231 0 6453 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 17 176 0 251 0 503 0 694 0 
COMMENT             958 0 1066 0 1153 0 1623 0 1820 0 2729 0 2919 0 2964 0 3155 0 3212 
COMMENT             0 4892 0 5155 0 6642 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "BanII" "grgcyc" 6 0 8 418 0 1688 0 2226 0 
COMMENT             2480 0 2522 0 3471 0 3968 0 5125 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 7 698 0 962 0 1070 0 2968 
COMMENT             0 3159 0 3216 0 4896 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 4 1103 1 3371 0 4138 1 
COMMENT             4420 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BbsI" "gaagac" 9 13 4 1108 1 3366 0 4143 1 
COMMENT             4415 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 2 1006 0 4518 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BbvI" "gcagc" 14 18 33 21 0 752 1 956 1 978 0 
COMMENT             1173 0 1324 1 1826 0 1944 0 2218 1 2390 0 2429 0 2534 1 3006 0 3009 
COMMENT             0 3261 1 3283 0 3418 1 3683 1 3939 1 4015 1 4111 1 4204 1 4867 1 
COMMENT             4889 0 5026 0 5550 1 6161 1 6552 0 6855 1 7061 1 7064 1 7154 0 7486 
COMMENT             1 0 "") 
COMMENT        #43=(CRSite (CStringList) "BccI" "ccatc" 10 11 16 133 0 468 0 898 1 1719 
COMMENT             1 1869 1 2053 0 2210 0 2951 1 3024 0 3284 1 4758 1 5181 1 5213 0 
COMMENT             6138 1 6425 1 6549 1 0 "") 
COMMENT        #44=(CRSite (CStringList) "BceAI" "acggc" 18 20 14 341 1 509 1 654 1 
COMMENT             1009 1 1054 1 1120 1 1171 1 1425 1 1527 1 1659 1 3169 0 3937 0 5169 
COMMENT             0 6983 1 0 "") 
COMMENT        #45=(CRSite (CStringList) "BcefI" "acggc" 18 19 14 342 1 510 1 655 1 
COMMENT             1010 1 1055 1 1121 1 1172 1 1426 1 1528 1 1660 1 3169 0 3937 0 5169 
COMMENT             0 6984 1 0 "") 
COMMENT        #46=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 2 4529 0 6057 1 0 
COMMENT             "") 
COMMENT        #47=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 5 1288 1 2761 0 3684 1 
COMMENT             5758 0 7285 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BfaI" "ctag" 2 0 12 131 0 256 0 529 0 591 0 
COMMENT             1400 0 2854 0 4345 0 4759 0 5043 0 6402 0 6737 0 6990 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 1 4551 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 6 43 0 2104 0 2131 0 
COMMENT             3462 0 4852 0 6477 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "BglII" "agatct" 2 0 1 4227 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 1 4562 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "Bme1580I" "gkgcmc" 6 0 13 181 0 1628 0 1699 0 
COMMENT             2226 0 2522 0 2726 0 2922 0 3828 0 3981 0 4299 0 5430 0 5927 0 7173 
COMMENT             0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 1 3401 1 0 "") 
COMMENT        #56=(CRSite (CStringList) "BmrI" "actggg" 12 11 3 289 0 4253 1 6555 0 0 
COMMENT             "") #57=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 2857 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BplI" "gagnnnnnctc" 25 0 3 1117 0 2349 0 4236 
COMMENT             0 0 "") 
COMMENT        #59=(CRSite (CStringList) "BpmI" "ctggag" 23 21 6 1255 1 2227 0 2385 1 
COMMENT             4275 1 4471 0 6526 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 5 545 1 1006 0 2324 0 
COMMENT             3333 1 4518 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 5 2502 0 5991 0 6859 0 
COMMENT             7100 1 7398 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 3 1529 0 4731 0 5196 0 0 
COMMENT             "") #63=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 13 57 0 695 0 959 0 1067 
COMMENT             0 1976 0 2965 0 3156 0 3213 0 4065 0 4833 0 4893 0 5673 0 6055 0 0 
COMMENT             "") 
COMMENT        #65=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 3 547 1 4624 0 6529 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 38 41 0 52 0 318 0 578 0 
COMMENT             879 0 1074 0 1110 0 1418 0 1478 0 1535 0 1571 0 1595 0 1757 0 2036 
COMMENT             0 2226 0 2300 0 2357 0 2426 0 2438 0 2439 0 2522 0 2558 0 2989 0 
COMMENT             3127 0 3151 0 3159 0 3160 0 3457 0 3919 0 3991 0 4023 0 4115 0 4275 
COMMENT             0 4494 0 4652 0 4836 0 4847 0 7323 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 5 173 0 1768 0 6299 0 
COMMENT             7130 0 7277 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 6 917 1 1133 1 
COMMENT             1419 0 2200 1 4708 0 5235 1 0 "") 
COMMENT        #69=(CRSite (CStringList) "BscAI" "gcatc" 10 12 15 428 0 1922 1 2072 1 
COMMENT             3306 0 4210 0 4248 0 4337 0 4892 1 4928 0 5444 1 5534 0 5897 1 6142 
COMMENT             0 6337 1 7389 1 0 "") 
COMMENT        #70=(CRSite (CStringList) "BseMII" "ctcag" 16 14 20 23 1 98 0 442 1 559 
COMMENT             0 610 0 1020 0 1545 1 2315 1 3347 0 4179 0 4532 0 4576 0 4649 0 
COMMENT             4796 1 4871 0 5447 0 6108 0 6625 1 6791 1 7200 1 0 "") 
COMMENT        #71=(CRSite (CStringList) "BseRI" "gaggag" 17 15 7 1109 0 1214 0 1731 1 
COMMENT             2388 1 2412 1 3740 1 3801 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BseYI" "cccagc" 2 6 6 1104 0 2212 1 2290 1 
COMMENT             2880 1 4669 1 7179 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 3 366 0 955 1 4476 0 0 
COMMENT             "") 
COMMENT        #74=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 7 147 0 169 0 1769 0 4747 
COMMENT             0 6077 0 6226 0 7149 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 8 1699 0 2976 0 3471 0 
COMMENT             3828 0 5430 0 5927 0 6012 0 7173 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 46 59 0 131 0 186 0 
COMMENT             187 0 261 0 323 0 324 0 452 0 500 0 568 0 921 0 1076 0 1093 0 1111 
COMMENT             0 1142 0 1578 0 1633 0 1634 0 1925 0 2208 0 2444 0 2445 0 2446 0 
COMMENT             2528 0 2600 0 3139 0 3334 0 3441 0 3805 0 3856 0 3955 0 4024 0 4116 
COMMENT             0 4127 0 4276 0 4494 0 4764 0 4836 0 4977 0 5303 0 5304 0 5557 0 
COMMENT             7005 0 7284 0 7450 0 7468 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 9 547 1 3380 0 3781 0 
COMMENT             4582 1 4624 0 5558 0 5600 1 5753 1 6529 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 4 3380 0 4582 1 5558 0 
COMMENT             5600 1 0 "") 
COMMENT        #80=(CRSite (CStringList) "BsmFI" "gggac" 16 20 7 197 1 1225 0 1554 1 
COMMENT             1573 0 3905 0 4065 1 4076 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 21 181 0 418 0 1628 0 
COMMENT             1688 0 1699 0 2226 0 2480 0 2522 0 2726 0 2922 0 2976 0 3471 0 3828 
COMMENT             0 3968 0 3981 0 4299 0 5125 0 5430 0 5927 0 6012 0 7173 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Bsp24I" "gacnnnnnntgg" 25 20 7 1583 1 1906 1 
COMMENT             2912 0 3860 1 5695 0 6789 1 6967 1 0 "") 
COMMENT        #84=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 20 24 1 97 0 443 1 558 
COMMENT             0 609 0 1019 0 1546 1 2316 1 3346 0 4178 0 4531 0 4575 0 4648 0 
COMMENT             4797 1 4870 0 5446 0 6107 0 6626 1 6792 1 7201 1 0 "") 
COMMENT        #85=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "BspHI" "tcatga" 2 0 6 2585 0 3004 0 3097 0 
COMMENT             5650 0 5755 0 6763 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "BspMI" "acctgc" 11 15 2 1808 1 2591 1 0 "") 
COMMENT        #88=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 4 1089 0 4743 1 5052 0 
COMMENT             5753 0 0 "") 
COMMENT        #89=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 7 2164 1 2410 1 2621 0 
COMMENT             3361 0 3862 1 6361 0 6535 1 0 "") 
COMMENT        #90=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 10 468 0 600 0 691 0 862 
COMMENT             0 1768 0 2125 0 2528 0 4127 0 5091 0 6510 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "BsrI" "actgg" 7 5 23 284 0 305 0 1228 1 1272 
COMMENT             1 1810 0 1899 1 2558 0 2901 0 2913 0 3167 1 3656 0 3857 0 4259 1 
COMMENT             4292 1 5285 0 5950 0 6120 1 6389 0 6432 0 6550 0 6956 0 7068 1 7081 
COMMENT             1 0 "") 
COMMENT        #92=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 828 0 4788 
COMMENT             0 4873 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "BssSI" "cacgag" 2 6 4 1668 1 5619 1 5926 0 
COMMENT             7310 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 1 1820 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 3589 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 3 1428 0 1599 0 4723 0 
COMMENT             0 "") 
COMMENT        #97=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 15 460 1 1083 1 1514 0 
COMMENT             1571 0 1934 0 1994 0 2576 0 2905 0 3313 0 3398 0 4240 1 5526 1 6149 
COMMENT             0 6436 0 6617 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "BstKTI" "gatc" 4 0 26 162 0 169 0 680 0 706 0 
COMMENT             1053 0 1661 0 1777 0 2015 0 2195 0 3228 0 4180 0 4230 0 4644 0 5915 
COMMENT             0 5951 0 5968 0 6226 0 6272 0 6290 0 6631 0 6736 0 6748 0 6826 0 
COMMENT             6834 0 6845 0 6920 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 25 1419 0 1573 0 1783 0 
COMMENT             1956 0 1981 0 2067 0 2559 0 2991 0 3128 0 3161 0 3239 0 3305 0 3329 
COMMENT             0 3407 0 3767 0 4049 0 4271 0 4277 0 4495 0 4544 0 4558 0 4612 0 
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COMMENT                               (CObjectList)) "CjeI: 16 sites") 26 20 
COMMENT                             "CCANNNNNNGTNNNNNNNNNNNNNNNN" 
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COMMENT                             "CCANNNNNNNTCNNNNNNNNNNNNNNN" 
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COMMENT                             "GGCGGANNNNNNNNNNNN" "CCGCCTNNNNNNNNNNNN") 
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COMMENT                             "GGTGANNNNNNNNN" "CCACTNNNNNNNNN") 
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COMMENT                             "GAACNNNNNCTCNNNNNNNNNNNNNN" 
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FEATURES             Location/Qualifiers
     LTR             1..141
                     /vntifkey="19"
                     /label=L-ITR
     misc_feature    3604..4212
                     /vntifkey="21"
                     /label=WPRE
     polyA_signal    4232..4710
                     /vntifkey="25"
                     /label=hGH\polyA
     LTR             4750..4890
                     /vntifkey="19"
                     /label=R-ITR
     rep_origin      4982..5288
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    5807..6664
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      6815..7482
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    708..741
                     /vntifkey="21"
                     /label=F3
     misc_feature    complement(3472..3505)
                     /vntifkey="21"
                     /label=F3\Rev
     misc_feature    792..825
                     /vntifkey="21"
                     /label=F5
     misc_feature    complement(3556..3589)
                     /vntifkey="21"
                     /label=F5\Rev
     misc_feature    complement(2873..3459)
                     /vntifkey="21"
                     /label=hM4DGi\Ex1
     misc_feature    complement(837..1205)
                     /vntifkey="21"
                     /label=mCherry\Exon2
     misc_feature    complement(1206..1214)
                     /vntifkey="21"
                     /label=CE\acceptor
     misc_feature    complement(1215..1273)
                     /vntifkey="21"
                     /label=CE\bp
     misc_feature    1280..1313
                     /vntifkey="21"
                     /label=lox\2722\Rev
     misc_feature    complement(2734..2767)
                     /vntifkey="21"
                     /label=lox\2722
     misc_feature    1364..1397
                     /vntifkey="21"
                     /label=lox\N\Rev
     misc_feature    complement(2818..2851)
                     /vntifkey="21"
                     /label=lox\N
     misc_feature    complement(2858..2872)
                     /vntifkey="21"
                     /label=CMV\intron\donor
     misc_feature    complement(1404..1417)
                     /vntifkey="21"
                     /label=CE\intron\donor
     misc_feature    complement(2631..2646)
                     /vntifkey="21"
                     /label=CMV\intron\acceptor
     misc_feature    complement(1418..1756)
                     /vntifkey="21"
                     /label=Mcherry\Exon1
     misc_feature    complement(1778..2630)
                     /vntifkey="21"
                     /label=hMD4Gi\Ex2
     promoter        156..701
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /vntifkey="29"
                     /label=EF1\promoter
     misc_feature    complement(2647..2727)
                     /vntifkey="21"
                     /label=CMV\intron\branch\point
BASE COUNT     1565 a      2006 c      2063 g      1853 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc 
      181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 
      241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 
      301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 
      361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 
      421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 
      481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 
      541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 
      601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 
      661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccgaa gttcctattc 
      721 ttcaaatagt ataggaactt cgcagagtag tagctggatt gtagctgcta ttagcaatct 
      781 aaaacctctt agaagttcct attcttcaaa aggtatagga acttcggcgc gccctattac 
      841 ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc 
      901 acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg 
      961 ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg 
     1021 ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg 
     1081 tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg 
     1141 gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag 
     1201 gagtcctgtg ggacgacatg acttaaccag ttaaccatgt tctctgaaca tgagaagggc 
     1261 tggccactct ccagtcgaca taacttcgta taggatactt tatacgaagt tatgcagaat 
     1321 ggtagctgga ttgtagctgc tattagcaat atgaaacctc ttaataactt cgtatagtat 
     1381 accttatacg aagttatact agtcctcctg tactcacctg ggtcacggtc accacgccgc 
     1441 cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt 
     1501 agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg 
     1561 acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct 
     1621 gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca 
     1681 cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct 
     1741 cctcgccctt gctcaccatg gtagcgaccg gtggatcccg cctggcagtg ccgatgttcc 
     1801 gatactggca cagcagcagg tgccggaagg tctttttaaa ggtggcgttg cacagagcat 
     1861 agcaggcagg gttgatggtg ctgttgacgt agcagagcca gtagccaatg gaccacaccg 
     1921 tgtcagggat gcagctctgg cagaaggtgt tcaccaggac catgacgttg tagggcgtcc 
     1981 aggtgaggat gaaggccagc agaatggcaa agatcgttcg tgtcactttg cgctcccggg 
     2041 ccgccatctg ccgcttcttg cgcacctggt tgcgagcgat gctggcgaac ttgcgggcca 
     2101 cgttggccgc agggcgcatg ccagccggcg tggcaggcac aatctcaatg gctgtcacac 
     2161 actcattgcc tgtctgcttc gtcacaatct ggatcttgga ccatctggag gctgggttga 
     2221 gggcccgcgg ctgcagggga ggggcgggca tggcgggcgt ggtggcctct gtggtggaca 
     2281 gctctgtggc tgggcgttcc ttggtgttct gggtggcact gcctgagctg gactcattgg 
     2341 aagtgtcctt atcagccacg gggcgcggtg gcggtggcag cgctggcggg ggggcctcct 
     2401 ccagcttgcc attgcgcagc tcctcccggg cggcctcccc gggcgggggc ttcttgacgc 
     2461 tctgcttcat tagtgggctc ttgaggaagg ccagcgtctt ggctttcttc tccttcgggc 
     2521 cctcgggccg gtgcttgtgg actcggctgc gactggccag ggagatgtgg atgtacagca 
     2581 ccgtcatgat gaccacaggc aggtagaagc cagcaatggc tgtgccaaag ctaaacacat 
     2641 gagaaataga gtcataagca catgggtcac atacaggaga tatgtatata acattaatac 
     2701 aattttataa aaaaaagggg gggcacaggt accataactt cgtataaagt atcctatacg 
     2761 aagttatttg ccttaaccca gaaattatca ctgttattct ttagaatggt gcaaagaata 
     2821 acttcgtata aggtatacta tacgaagtta tgctagcaac cccgacactt acgtcactgc 
     2881 tgggttggac aggaactgga tgaagcactg gttgtcgggc accgtccgct tacccaccac 
     2941 aaactgccag aacaagatgg caggcgccca gagcacgaag gacagtaccc aggcagcagc 
     3001 aatcatgagg cctgccatct tggtggtgcg ccgggcaggg taggtgagag gcttggtgac 
     3061 gcagaagtag cggtcaaagc tgatgatgag aaggttcatg acggaggcgt tgctcaccac 
     3121 gcagtccagg gccagccaca ggtcgcagac cacggcgccc aggggccagt agcccttgat 
     3181 gatgtacacg gtgtagaggt tcatggagaa ggcgcctatg atgagatcag cacacgccag 
     3241 gctgaagagg aagtagttgt tgactgtctg cagctgcctg ttgaccttga tggacagcat 
     3301 caccaggatg ttgcccacga cagtcaccag gctcagggag cctgtcactg tggcaatgaa 
     3361 gaccatttcc accgtctcat agcgattgtg ggatgatgac gtgaccaggc gcacggactg 
     3421 attgcccgag ctgccattga caggtgtgaa gttggccatg gtggcgagct cgaagttcct 
     3481 atactatttg aagaatagga acttcttgcc ttaacccaga aattatcact gttattcttt 
     3541 agaatggtgc aaagagaagt tcctatacct tttgaagaat aggaacttcg aattcgatat 
     3601 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 
     3661 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 
     3721 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 
     3781 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 
     3841 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 
     3901 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 
     3961 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt 
     4021 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 
     4081 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 
     4141 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 
     4201 gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca 
     4261 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa 
     4321 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg 
     4381 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg 
     4441 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt 
     4501 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg 
     4561 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc 
     4621 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag 
     4681 gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg 
     4741 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc 
     4801 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag 
     4861 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc 
     4921 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg 
     4981 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 
     5041 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 
     5101 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 
     5161 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac 
     5221 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 
     5281 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat 
     5341 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 
     5401 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata 
     5461 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 
     5521 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 
     5581 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 
     5641 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 
     5701 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
     5761 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 
     5821 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 
     5881 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 
     5941 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 
     6001 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 
     6061 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 
     6121 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 
     6181 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 
     6241 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 
     6301 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 
     6361 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 
     6421 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 
     6481 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 
     6541 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 
     6601 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 
     6661 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 
     6721 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
     6781 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     6841 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     6901 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     6961 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 
     7021 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     7081 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     7141 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     7201 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     7261 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     7321 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     7381 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     7441 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt 
//
