LOCUS       Exported                8456 bp ds-DNA     circular SYN 06-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 6, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..138
                     /label=Spn promoter
                     /note="/ApEinfo_fwdcolor=pink 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=Spn 
                     promoter /vntifkey=30"
     CDS             139..1149
                     /codon_start=1
                     /gene="Streptomycin/Spectinomycin adenyltransferase"
                     /label=SpnR
                     /note="/ApEinfo_fwdcolor=pink 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=SpnR 
                     /invitrogen=2750000 /vntifkey=4"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1216..1889
                     /gene="ori"
                     /label=pUC origin
                     /note="/ApEinfo_fwdcolor=pink 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=pUC 
                     origin /invitrogen=2530000 /vntifkey=33"
     terminator      2319..2362
                     /label=rrnB T1 terminatior
                     /note="/ApEinfo_fwdcolor=pink 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=rrnB T1
                     terminatior /vntifkey=43"
     primer_bind     2429..2444
                     /label=M13 (-20) forward primer
     misc_recomb     2461..2543
                     /label=attL1
                     /note="/ApEinfo_fwdcolor=#00ff40 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=attL1 
                     /invitrogen=2670000 /vntifkey=86"
     protein_bind    2544..2560
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2567..2587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2594..3181
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             3260..7531
                     /codon_start=1
                     /label=zCas9 fusion protein
                     /label=zCas9
                     /note="/dnas_title=zCas9 /vntifkey=4"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAADKKYS
                     IGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAERTRLK
                     RTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVD
                     EVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDK
                     LFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI
                     ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL
                     LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYA
                     GYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGEL
                     HAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNF
                     EEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKP
                     AFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHD
                     LLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRR
                     YTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVS
                     GQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
                     QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINR
                     LSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKL
                     ITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI
                     REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFV
                     YGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGE
                     TGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIRPKRNSDKLIARKKDWD
                     PKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKG
                     YKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNELALPSKYVNFLYLASHYEKL
                     KGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE
                     QAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLDATLIHQSITGLYETRIDLS
                     QLGGDKRPAATKKAGQAKKKK"
     sig_peptide     3263..3328
                     /label=3xFLAG
     misc_feature    3335..3355
                     /label=SV40 NLS
     CDS             3380..7480
                     /codon_start=1
                     /label=zCas9
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    3404..3405
                     /label=D10A
     misc_feature    3523..3542
                     /label=A61R-R
     misc_feature    3530..3550
                     /label=A61R-R2
     misc_feature    3535..3553
                     /label=A61R-R3
     misc_feature    3543..3562
                     /label=A61R-F
     misc_feature    3551..3571
                     /label=A61R-F2
     misc_feature    3554..3573
                     /label=A61R-F3
     misc_feature    3557..3559
                     /label=A61R
     3'UTR           5894..5896
                     /label=H840A
     misc_feature    6707..6709
                     /label=L1111R
     misc_feature    6779..6781
                     /label=D1135L
     misc_feature    6782..6784
                     /label=S1136W
     misc_feature    7028..7030
                     /label=G1218K
     misc_feature    7031..7033
                     /label=E1219Q
     misc_feature    7325..7327
                     /label=N1317R
     misc_feature    7340..7342
                     /label=A1322R
     misc_feature    7373..7375
                     /label=R1333P
     misc_feature    7379..7381
                     /label=R1335Q
     misc_feature    7385..7387
                     /label=T1337R
     misc_feature    7481..7528
                     /label=nucleoplasmin NLS
     terminator      7536..8156
                     /label=rbcS-E9t
                     /note="/dnas_title=rbcS-E9t /vntifkey=43"
     misc_recomb     8203..8326
                     /label=attR5
                     /note="/ApEinfo_fwdcolor=#00ff00 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=attR5 
                     /invitrogen=2703000 /vntifkey=86"
     primer_bind     complement(8367..8383)
                     /label=M13 reverse primer
     promoter        8455..8456
                     /label=Spn promoter
                     /note="/ApEinfo_fwdcolor=pink 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=Spn 
                     promoter /vntifkey=30"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgatgccaa aaaaaaagcg
     2581 gaaggttagt tcacaactgg tgaagtctga acttgaggag aagaagagcg agttgaggca
     2641 caagttgaag tatgtccccc atgaatacat cgagttgatt gagattgctc gcaacagcac
     2701 ccaggaccgg attctcgaaa tgaaggtcat ggaatttttt atgaaagtgt acgggtaccg
     2761 cggaaagcat ctcgggggaa gtcgcaaacc tgacggggct atctatacgg tgggttctcc
     2821 tatagactac ggggtcattg tggatacgaa ggcctatagt ggcggataca atctgccaat
     2881 tggacaagct gatgaaatgc agaggtacgt tgaagagaat cagacccgca acaaacatat
     2941 aaacccaaat gaatggtgga aggtgtaccc cagcagtgtc accgaattca aatttctgtt
     3001 tgtgagtggg catttcaaag gaaactataa agcacaactt acgagactga accatattac
     3061 caattgtaac ggcgctgtgt tgagtgtcga ggagctgctc atagggggag aaatgatcaa
     3121 agcaggcaca ttgactctcg aggaggtgcg gcggaagttt aacaatgggg aaatcaactt
     3181 cggtggtggt ggttctggag gtggaggctc tggaggagga ggcagcggtg gaggcgggtc
     3241 tggaggtggt ggttctacca tggattacaa ggaccacgac ggggattaca aggaccacga
     3301 cattgattac aaggatgatg atgacaagat ggctccgaag aagaagagga aggttggcat
     3361 ccacggggtg ccagctgctg acaagaagta ctcgatcggc ctcgccattg ggactaactc
     3421 tgttggctgg gccgtgatca ccgacgagta caaggtgccc tcaaagaagt tcaaggtcct
     3481 gggcaacacc gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc tgttcgacag
     3541 cggcgagacg gctgagagga cgcggctcaa gcgcaccgcc cgcaggcggt acacgcgcag
     3601 gaagaatcgc atctgctacc tgcaggagat tttctccaac gagatggcga aggttgacga
     3661 ttctttcttc cacaggctgg aggagtcatt cctcgtggag gaggataaga agcacgagcg
     3721 gcatccaatc ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt accctacgat
     3781 ctaccatctg cggaagaagc tcgtggactc cacagataag gcggacctcc gcctgatcta
     3841 cctcgctctg gcccacatga ttaagttcag gggccatttc ctgatcgagg gggatctcaa
     3901 cccggacaat agcgatgttg acaagctgtt catccagctc gtgcagacgt acaaccagct
     3961 cttcgaggag aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc tgtccgctag
     4021 gctctcgaag tctcggcgcc tcgagaacct gatcgcccag ctgccgggcg agaagaagaa
     4081 cggcctgttc gggaatctca ttgcgctcag cctggggctc acgcccaact tcaagtcgaa
     4141 tttcgatctc gctgaggacg ccaagctgca gctctccaag gacacatacg acgatgacct
     4201 ggataacctc ctggcccaga tcggcgatca gtacgcggac ctgttcctcg ctgccaagaa
     4261 tctgtcggac gccatcctcc tgtctgatat tctcagggtg aacaccgaga ttacgaaggc
     4321 tccgctctca gcctccatga tcaagcgcta cgacgagcac catcaggatc tgaccctcct
     4381 gaaggcgctg gtcaggcagc agctccccga gaagtacaag gagatcttct tcgatcagtc
     4441 gaagaacggc tacgctgggt acattgacgg cggggcctct caggaggagt tctacaagtt
     4501 catcaagccg attctggaga agatggacgg cacggaggag ctgctggtga agctcaatcg
     4561 cgaggacctc ctgaggaagc agcggacatt cgataacggc agcatcccac accagattca
     4621 tctcggggag ctgcacgcta tcctgaggag gcaggaggac ttctaccctt tcctcaagga
     4681 taaccgcgag aagatcgaga agattctgac tttcaggatc ccgtactacg tcggcccact
     4741 cgctaggggc aactcccgct tcgcttggat gacccgcaag tcagaggaga cgatcacgcc
     4801 gtggaacttc gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca tcgagaggat
     4861 gacgaatttc gacaagaacc tgccaaatga gaaggtgctc cctaagcact cgctcctgta
     4921 cgagtacttc acagtctaca acgagctgac taaggtgaag tatgtgaccg agggcatgag
     4981 gaagccggct ttcctgtctg gggagcagaa gaaggccatc gtggacctcc tgttcaagac
     5041 caaccggaag gtcacggtta agcagctcaa ggaggactac ttcaagaaga ttgagtgctt
     5101 cgattcggtc gagatctctg gcgttgagga ccgcttcaac gcctccctgg ggacctacca
     5161 cgatctcctg aagatcatta aggataagga cttcctggac aacgaggaga atgaggatat
     5221 cctcgaggac attgtgctga cactcactct gttcgaggac cgggagatga tcgaggagcg
     5281 cctgaagact tacgcccatc tcttcgatga caaggtcatg aagcagctca agaggaggag
     5341 gtacaccggc tgggggaggc tgagcaggaa gctcatcaac ggcattcggg acaagcagtc
     5401 cgggaagacg atcctcgact tcctgaagag cgatggcttc gcgaaccgca atttcatgca
     5461 gctgattcac gatgacagcc tcacattcaa ggaggatatc cagaaggctc aggtgagcgg
     5521 ccagggggac tcgctgcacg agcatatcgc gaacctcgct ggctcgccag ctatcaagaa
     5581 ggggattctg cagaccgtga aggttgtgga cgagctggtg aaggtcatgg gcaggcacaa
     5641 gcctgagaac atcgtcattg agatggcccg ggagaatcag accacgcaga agggccagaa
     5701 gaactcacgc gagaggatga agaggatcga ggagggcatt aaggagctgg ggtcccagat
     5761 cctcaaggag cacccggtgg agaacacgca gctgcagaat gagaagctct acctgtacta
     5821 cctccagaat ggccgcgata tgtatgtgga ccaggagctg gatattaaca ggctcagcga
     5881 ttacgacgtc gatgctatcg ttccacagtc attcctgaag gatgactcca ttgacaacaa
     5941 ggtcctcacc aggtcggaca agaaccgggg caagtctgat aatgttcctt cagaggaggt
     6001 cgttaagaag atgaagaact actggcgcca gctcctgaat gccaagctga tcacgcagcg
     6061 gaagttcgat aacctcacaa aggctgagag gggcgggctc tctgagctgg acaaggcggg
     6121 cttcatcaag aggcagctgg tcgagacacg gcagatcact aagcacgttg cgcagattct
     6181 cgactcacgg atgaacacta agtacgatga gaatgacaag ctgatccgcg aggtgaaggt
     6241 catcaccctg aagtcaaagc tcgtctccga cttcaggaag gatttccagt tctacaaggt
     6301 tcgggagatc aacaattacc accatgccca tgacgcgtac ctgaacgcgg tggtcggcac
     6361 agctctgatc aagaagtacc caaagctcga gagcgagttc gtgtacgggg actacaaggt
     6421 ttacgatgtg aggaagatga tcgccaagtc ggagcaggag attggcaagg ctaccgccaa
     6481 gtacttcttc tactctaaca ttatgaattt cttcaagaca gagatcactc tggccaatgg
     6541 cgagatccgg aagcgccccc tcatcgagac gaacggcgag acgggggaga tcgtgtggga
     6601 caagggcagg gatttcgcga ccgtcaggaa ggttctctcc atgccacaag tgaatatcgt
     6661 caagaagaca gaggtccaga ctggcgggtt ctctaaggag tcaattaggc ctaagcggaa
     6721 cagcgacaag ctcatcgccc gcaagaagga ctgggatccg aagaagtacg gcgggttcct
     6781 gtggcccact gtggcctact cggtcctggt tgtggcgaag gttgagaagg gcaagtccaa
     6841 gaagctcaag agcgtgaagg agctgctggg gatcacgatt atggagcgct ccagcttcga
     6901 gaagaacccg atcgatttcc tggaggcgaa gggctacaag gaggtgaaga aggacctgat
     6961 cattaagctc cccaagtact cactcttcga gctggagaac ggcaggaagc ggatgctggc
     7021 ttccgctaag cagctgcaga aggggaacga gctggctctg ccgtccaagt atgtgaactt
     7081 cctctacctg gcctcccact acgagaagct caagggcagc cccgaggaca acgagcagaa
     7141 gcagctgttc gtcgagcagc acaagcatta cctcgacgag atcattgagc agatttccga
     7201 gttctccaag cgcgtgatcc tggccgacgc gaatctggat aaggtcctct ccgcgtacaa
     7261 caagcaccgc gacaagccaa tcagggagca ggctgagaat atcattcatc tcttcaccct
     7321 gacgaggctc ggcgccccta gggctttcaa gtacttcgac acaactatcg atccgaagca
     7381 gtacaggagc actaaggagg tcctggacgc gaccctcatc caccagtcga ttaccggcct
     7441 ctacgagacg cgcatcgacc tgtctcagct cgggggcgac aagcggccag cggcgacgaa
     7501 gaaggcgggg caggcgaaga agaagaagtg agctcagagc tttcgttcgt atcatcggtt
     7561 tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc gcacacacca gaatcctact
     7621 gagtttgagt attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta
     7681 atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga
     7741 agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc
     7801 tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag
     7861 taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt
     7921 gtagttgtac cattatgctt attcactagg caacaaatat attttcagac ctagaaaagc
     7981 tgcaaatgtt actgaataca agtatgtcct cttgtgtttt agacatttat gaactttcct
     8041 ttatgtaatt ttccagaatc cttgtcagat tctaatcatt gctttataat tatagttata
     8101 ctcatggatt tgtagttgag tatgaaaata ttttttaatg cattttatga cttgccaatt
     8161 gttccggaac tctagataag cttaccggaa agggcgaatt cgcaactttg tatacaaaag
     8221 ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     8281 aacagactac ataatactgt aaaacacaac atatccagtc actatgccat ccagctgata
     8341 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     8401 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctc
//
