LOCUS       Exported                8429 bp ds-DNA     circular SYN 06-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 6, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..138
                     /label=Spn promoter
                     /note="/ApEinfo_fwdcolor=pink 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=Spn 
                     promoter /vntifkey=30"
     CDS             139..1149
                     /codon_start=1
                     /gene="Streptomycin/Spectinomycin adenyltransferase"
                     /label=SpnR
                     /note="/ApEinfo_fwdcolor=pink 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=SpnR 
                     /invitrogen=2750000 /vntifkey=4"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1216..1889
                     /gene="ori"
                     /label=pUC origin
                     /note="/ApEinfo_fwdcolor=pink 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=pUC 
                     origin /invitrogen=2530000 /vntifkey=33"
     terminator      2319..2362
                     /label=rrnB T1 terminatior
                     /note="/ApEinfo_fwdcolor=pink 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=rrnB T1
                     terminatior /vntifkey=43"
     primer_bind     2429..2444
                     /label=M13 (-20) forward primer
     misc_recomb     2461..2543
                     /label=attL1
                     /note="/ApEinfo_fwdcolor=#00ff40 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=attL1 
                     /invitrogen=2670000 /vntifkey=86"
     protein_bind    2544..2560
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2567..2587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2594..3181
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             3233..7504
                     /codon_start=1
                     /label=zCas9 fusion protein
                     /label=zCas9
                     /note="/dnas_title=zCas9 /vntifkey=4"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAADKKYS
                     IGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAERTRLK
                     RTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVD
                     EVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDK
                     LFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI
                     ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL
                     LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYA
                     GYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGEL
                     HAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNF
                     EEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKP
                     AFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHD
                     LLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRR
                     YTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVS
                     GQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
                     QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINR
                     LSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKL
                     ITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI
                     REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFV
                     YGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGE
                     TGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIRPKRNSDKLIARKKDWD
                     PKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKG
                     YKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNELALPSKYVNFLYLASHYEKL
                     KGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE
                     QAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLDATLIHQSITGLYETRIDLS
                     QLGGDKRPAATKKAGQAKKKK"
     sig_peptide     3236..3301
                     /label=3xFLAG
     misc_feature    3308..3328
                     /label=SV40 NLS
     CDS             3353..7453
                     /codon_start=1
                     /label=zCas9
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     5'UTR           3377..3378
                     /label=D10A
     misc_feature    3496..3515
                     /label=A61R-R
     misc_feature    3503..3523
                     /label=A61R-R2
     misc_feature    3508..3526
                     /label=A61R-R3
     misc_feature    3516..3535
                     /label=A61R-F
     misc_feature    3524..3544
                     /label=A61R-F2
     misc_feature    3527..3546
                     /label=A61R-F3
     misc_feature    3530..3532
                     /label=A61R
     misc_feature    5867..5869
                     /label=H840A
     misc_feature    6680..6682
                     /label=L1111R
     misc_feature    6752..6754
                     /label=D1135L
     misc_feature    6755..6757
                     /label=S1136W
     misc_feature    7001..7003
                     /label=G1218K
     misc_feature    7004..7006
                     /label=E1219Q
     misc_feature    7298..7300
                     /label=N1317R
     misc_feature    7313..7315
                     /label=A1322R
     misc_feature    7346..7348
                     /label=R1333P
     misc_feature    7352..7354
                     /label=R1335Q
     misc_feature    7358..7360
                     /label=T1337R
     misc_feature    7383
                     /label=T
     misc_feature    7454..7501
                     /label=nucleoplasmin NLS
     terminator      7509..8129
                     /label=rbcS-E9t
                     /note="/dnas_title=rbcS-E9t /vntifkey=43"
     misc_recomb     8176..8299
                     /label=attR5
                     /note="/ApEinfo_fwdcolor=#00ff00 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=attR5 
                     /invitrogen=2703000 /vntifkey=86"
     primer_bind     complement(8340..8356)
                     /label=M13 reverse primer
     primer_bind     complement(8357..8379)
                     /label=M13-R1
     promoter        8428..8429
                     /label=Spn promoter
                     /note="/ApEinfo_fwdcolor=pink 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=Spn 
                     promoter /vntifkey=30"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgatgccaa aaaaaaagcg
     2581 gaaggttagt tcacaactgg tgaagtctga acttgaggag aagaagagcg agttgaggca
     2641 caagttgaag tatgtccccc atgaatacat cgagttgatt gagattgctc gcaacagcac
     2701 ccaggaccgg attctcgaaa tgaaggtcat ggaatttttt atgaaagtgt acgggtaccg
     2761 cggaaagcat ctcgggggaa gtcgcaaacc tgacggggct atctatacgg tgggttctcc
     2821 tatagactac ggggtcattg tggatacgaa ggcctatagt ggcggataca atctgccaat
     2881 tggacaagct gatgaaatgc agaggtacgt tgaagagaat cagacccgca acaaacatat
     2941 aaacccaaat gaatggtgga aggtgtaccc cagcagtgtc accgaattca aatttctgtt
     3001 tgtgagtggg catttcaaag gaaactataa agcacaactt acgagactga accatattac
     3061 caattgtaac ggcgctgtgt tgagtgtcga ggagctgctc atagggggag aaatgatcaa
     3121 agcaggcaca ttgactctcg aggaggtgcg gcggaagttt aacaatgggg aaatcaactt
     3181 ctcagggagt gagacgccag gaacatcaga aagcgcaacc ccggagtcga ccatggatta
     3241 caaggaccac gacggggatt acaaggacca cgacattgat tacaaggatg atgatgacaa
     3301 gatggctccg aagaagaaga ggaaggttgg catccacggg gtgccagctg ctgacaagaa
     3361 gtactcgatc ggcctcgcca ttgggactaa ctctgttggc tgggccgtga tcaccgacga
     3421 gtacaaggtg ccctcaaaga agttcaaggt cctgggcaac accgatcggc attccatcaa
     3481 gaagaatctc attggcgctc tcctgttcga cagcggcgag acggctgaga ggacgcggct
     3541 caagcgcacc gcccgcaggc ggtacacgcg caggaagaat cgcatctgct acctgcagga
     3601 gattttctcc aacgagatgg cgaaggttga cgattctttc ttccacaggc tggaggagtc
     3661 attcctcgtg gaggaggata agaagcacga gcggcatcca atcttcggca acattgtcga
     3721 cgaggttgcc taccacgaga agtaccctac gatctaccat ctgcggaaga agctcgtgga
     3781 ctccacagat aaggcggacc tccgcctgat ctacctcgct ctggcccaca tgattaagtt
     3841 caggggccat ttcctgatcg agggggatct caacccggac aatagcgatg ttgacaagct
     3901 gttcatccag ctcgtgcaga cgtacaacca gctcttcgag gagaacccca ttaatgcgtc
     3961 aggcgtcgac gcgaaggcta tcctgtccgc taggctctcg aagtctcggc gcctcgagaa
     4021 cctgatcgcc cagctgccgg gcgagaagaa gaacggcctg ttcgggaatc tcattgcgct
     4081 cagcctgggg ctcacgccca acttcaagtc gaatttcgat ctcgctgagg acgccaagct
     4141 gcagctctcc aaggacacat acgacgatga cctggataac ctcctggccc agatcggcga
     4201 tcagtacgcg gacctgttcc tcgctgccaa gaatctgtcg gacgccatcc tcctgtctga
     4261 tattctcagg gtgaacaccg agattacgaa ggctccgctc tcagcctcca tgatcaagcg
     4321 ctacgacgag caccatcagg atctgaccct cctgaaggcg ctggtcaggc agcagctccc
     4381 cgagaagtac aaggagatct tcttcgatca gtcgaagaac ggctacgctg ggtacattga
     4441 cggcggggcc tctcaggagg agttctacaa gttcatcaag ccgattctgg agaagatgga
     4501 cggcacggag gagctgctgg tgaagctcaa tcgcgaggac ctcctgagga agcagcggac
     4561 attcgataac ggcagcatcc cacaccagat tcatctcggg gagctgcacg ctatcctgag
     4621 gaggcaggag gacttctacc ctttcctcaa ggataaccgc gagaagatcg agaagattct
     4681 gactttcagg atcccgtact acgtcggccc actcgctagg ggcaactccc gcttcgcttg
     4741 gatgacccgc aagtcagagg agacgatcac gccgtggaac ttcgaggagg tggtcgacaa
     4801 gggcgctagc gctcagtcgt tcatcgagag gatgacgaat ttcgacaaga acctgccaaa
     4861 tgagaaggtg ctccctaagc actcgctcct gtacgagtac ttcacagtct acaacgagct
     4921 gactaaggtg aagtatgtga ccgagggcat gaggaagccg gctttcctgt ctggggagca
     4981 gaagaaggcc atcgtggacc tcctgttcaa gaccaaccgg aaggtcacgg ttaagcagct
     5041 caaggaggac tacttcaaga agattgagtg cttcgattcg gtcgagatct ctggcgttga
     5101 ggaccgcttc aacgcctccc tggggaccta ccacgatctc ctgaagatca ttaaggataa
     5161 ggacttcctg gacaacgagg agaatgagga tatcctcgag gacattgtgc tgacactcac
     5221 tctgttcgag gaccgggaga tgatcgagga gcgcctgaag acttacgccc atctcttcga
     5281 tgacaaggtc atgaagcagc tcaagaggag gaggtacacc ggctggggga ggctgagcag
     5341 gaagctcatc aacggcattc gggacaagca gtccgggaag acgatcctcg acttcctgaa
     5401 gagcgatggc ttcgcgaacc gcaatttcat gcagctgatt cacgatgaca gcctcacatt
     5461 caaggaggat atccagaagg ctcaggtgag cggccagggg gactcgctgc acgagcatat
     5521 cgcgaacctc gctggctcgc cagctatcaa gaaggggatt ctgcagaccg tgaaggttgt
     5581 ggacgagctg gtgaaggtca tgggcaggca caagcctgag aacatcgtca ttgagatggc
     5641 ccgggagaat cagaccacgc agaagggcca gaagaactca cgcgagagga tgaagaggat
     5701 cgaggagggc attaaggagc tggggtccca gatcctcaag gagcacccgg tggagaacac
     5761 gcagctgcag aatgagaagc tctacctgta ctacctccag aatggccgcg atatgtatgt
     5821 ggaccaggag ctggatatta acaggctcag cgattacgac gtcgatgcta tcgttccaca
     5881 gtcattcctg aaggatgact ccattgacaa caaggtcctc accaggtcgg acaagaaccg
     5941 gggcaagtct gataatgttc cttcagagga ggtcgttaag aagatgaaga actactggcg
     6001 ccagctcctg aatgccaagc tgatcacgca gcggaagttc gataacctca caaaggctga
     6061 gaggggcggg ctctctgagc tggacaaggc gggcttcatc aagaggcagc tggtcgagac
     6121 acggcagatc actaagcacg ttgcgcagat tctcgactca cggatgaaca ctaagtacga
     6181 tgagaatgac aagctgatcc gcgaggtgaa ggtcatcacc ctgaagtcaa agctcgtctc
     6241 cgacttcagg aaggatttcc agttctacaa ggttcgggag atcaacaatt accaccatgc
     6301 ccatgacgcg tacctgaacg cggtggtcgg cacagctctg atcaagaagt acccaaagct
     6361 cgagagcgag ttcgtgtacg gggactacaa ggtttacgat gtgaggaaga tgatcgccaa
     6421 gtcggagcag gagattggca aggctaccgc caagtacttc ttctactcta acattatgaa
     6481 tttcttcaag acagagatca ctctggccaa tggcgagatc cggaagcgcc ccctcatcga
     6541 gacgaacggc gagacggggg agatcgtgtg ggacaagggc agggatttcg cgaccgtcag
     6601 gaaggttctc tccatgccac aagtgaatat cgtcaagaag acagaggtcc agactggcgg
     6661 gttctctaag gagtcaatta ggcctaagcg gaacagcgac aagctcatcg cccgcaagaa
     6721 ggactgggat ccgaagaagt acggcgggtt cctgtggccc actgtggcct actcggtcct
     6781 ggttgtggcg aaggttgaga agggcaagtc caagaagctc aagagcgtga aggagctgct
     6841 ggggatcacg attatggagc gctccagctt cgagaagaac ccgatcgatt tcctggaggc
     6901 gaagggctac aaggaggtga agaaggacct gatcattaag ctccccaagt actcactctt
     6961 cgagctggag aacggcagga agcggatgct ggcttccgct aagcagctgc agaaggggaa
     7021 cgagctggct ctgccgtcca agtatgtgaa cttcctctac ctggcctccc actacgagaa
     7081 gctcaagggc agccccgagg acaacgagca gaagcagctg ttcgtcgagc agcacaagca
     7141 ttacctcgac gagatcattg agcagatttc cgagttctcc aagcgcgtga tcctggccga
     7201 cgcgaatctg gataaggtcc tctccgcgta caacaagcac cgcgacaagc caatcaggga
     7261 gcaggctgag aatatcattc atctcttcac cctgacgagg ctcggcgccc ctagggcttt
     7321 caagtacttc gacacaacta tcgatccgaa gcagtacagg agcactaagg aggtcctgga
     7381 cgcgaccctc atccaccagt cgattaccgg cctctacgag acgcgcatcg acctgtctca
     7441 gctcgggggc gacaagcggc cagcggcgac gaagaaggcg gggcaggcga agaagaagaa
     7501 gtgagctcag agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca
     7561 tcagtttcat tgcgcacaca ccagaatcct actgagtttg agtattatgg cattgggaaa
     7621 actgtttttc ttgtaccatt tgttgtgctt gtaatttact gtgtttttta ttcggttttc
     7681 gctatcgaac tgtgaaatgg aaatggatgg agaagagtta atgaatgata tggtcctttt
     7741 gttcattctc aaattaatat tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa
     7801 attataagag atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta
     7861 atgaccgaag ttaatatgag gagtaaaaca cttgtagttg taccattatg cttattcact
     7921 aggcaacaaa tatattttca gacctagaaa agctgcaaat gttactgaat acaagtatgt
     7981 cctcttgtgt tttagacatt tatgaacttt cctttatgta attttccaga atccttgtca
     8041 gattctaatc attgctttat aattatagtt atactcatgg atttgtagtt gagtatgaaa
     8101 atatttttta atgcatttta tgacttgcca attgttccgg aactctagat aagcttaccg
     8161 gaaagggcga attcgcaact ttgtatacaa aagttgaacg agaaacgtaa aatgatataa
     8221 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
     8281 aacatatcca gtcactatgc catccagctg atatccccta tagtgagtcg tattacatgg
     8341 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     8401 aagataaaaa tatatcatca tgcctcctc
//
