LOCUS       pCMV(MinDis)-iCyt_BrEt_ 6655 bp ds-DNA     circular     06-OCT-2021
DEFINITION  .
ACCESSION   urn:local:.:1506620526970.23                                       
FEATURES             Location/Qualifiers
     misc_feature    2..597
                     /label="hCMV promoter/enhancer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    545..549
                     /label="CAAAT"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    578..584
                     /label="TATA box"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     637..656
                     /label="T7 promoter/priming site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     sig_peptide     736..786
                     /label="sig_peptide"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    739..785
                     /label="Geneious type: Editing History Replacement"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    787..792
                     /label="Geneious type: restriction site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    788..791
                     /label="ligation"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    788..792
                     /label="misc_feature"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             793..2358
                     /label="Translation 793-2358"
     misc_feature    2359..2360
                     /label="ligation"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2359..2361
                     /label="Geneious type: restriction site"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2361..2401
                     /label="motif"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     sig_peptide     2362..2382
                     /label="sig_peptide"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2362..2385
                     /label="Geneious type: Editing History Replacement"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     modified_base   2383..2385
                     /label="modified_base"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(2404..2421)
                     /label="r.0.401"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(2469..2485)
                     /label="motif"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      complement(2748..3430)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(5208..5867)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            complement(5208..5867)
                     /label="Translation 5867-5208"
     rep_origin      complement(6197..6653)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 cgcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagaa ctctctggct
      601 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      661 ccaagcttgg taccgaactc ggatccacta gtaacggccg ccagtgtgct ggaattcggc
      721 ttggggatat ccaccatgct gctatccgtg ccgctgctgc tcggcctcct cggcctggcc
      781 atcgccagat ctGCGAACGA CACCGTAGTT GTGGGCTCGA TCATCTTTAC AGAAGGGATT
      841 ATCGTCGCAA ACATGGTGGC AGAGATGATT GAGGCGCATA CAGACCTTAA GGTGGTTCGC
      901 AAACTGAACC TTGGCGGGCA GGGTGTTAAC TTTGAAGCCA TTAAACGCGG AGGTGCGAAT
      961 AATGGTATTG ACATTTACGT GGAGTACACT GGGCACGGTC TTGTGGATAT TCTGGGGTTC
     1021 CCGGAGCCGA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCGAACTTC
     1081 AAGATCCGCC ACAACGTGGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
     1141 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC
     1201 GTGCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
     1261 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG GCGGTACCGG AGGGAGCATG
     1321 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
     1381 GTAAACGGCC ACAAGTTCAG CGTGCGCGGC GAGGGCGAGG GCGATGCCAC CAACGGCAAG
     1441 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
     1501 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
     1561 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CAGCTTCAAG
     1621 GACGACGGCA CCTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
     1681 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
     1741 GAGTACAACT TTCCGCCGCC CGCGACTACT GATCCAGAAG GTGCATACGA AACCGTGAAG
     1801 AAGGAGTACA AACGTAAATG GAATATTGTA TGGCTCAAAC CACTGGGATT CAACAATACG
     1861 TATACGCTTA CCGTTAAAGA CGAACTGGCG AAACAGTATA ACCTTAAAAC CTTCAGTGAC
     1921 TTAGCGAAAA TCTCGGATAA GCTGATTCTG GGTGCAACGA TGTGGTTTTT AGAAGGGCCC
     1981 GATGGTTACC CAGGCCTGCA AAAACTGTAC AATTTCAAAT TCAAGCACAC CAAAAGCATG
     2041 GACATGGGTA TTCGCTATAC CGCCATTGAT AATAACGAAG TTCAGGTAAT TGATGCCTGG
     2101 GCCACTGATG GCTTGCTGGT GAGCCACAAA TTAAAAATTC TGGAGGATGA TAAAGCGTTC
     2161 TTCCCGCCGT ATTATGCTGC CCCCATCATC CGTCAGGATG TCTTAGATAA GCATCCTGAA
     2221 CTGAAGGACG TGCTGAACAA ACTCGCGAAT CAAATTTCAC TGGAAGAAAT GCAGAAACTG
     2281 AATTACAAGG TGGACGGTGA GGGTCAGGAC CCAGCGAAAG TAGCTAAGGA GTTTTTGAAA
     2341 GAGAAAGGTt taattctgca gacagcagaa aaagatgagt tgtgagcggc cgctcgagat
     2401 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     2461 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     2521 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     2581 gggaggattg ggaagacaat agcaggcgtg ctggggatgc ggtgggctct atggcttctg
     2641 aggcggaaag aaccagtggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2701 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2761 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2821 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2881 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2941 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3001 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3061 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3121 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3181 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     3241 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3301 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3361 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3421 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3481 tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc tgccgccccg
     3541 acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa
     3601 gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc
     3661 tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt attctgtctt
     3721 tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc
     3781 ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag
     3841 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg
     3901 aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg
     3961 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     4021 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     4081 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
     4141 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     4201 cgacgagatc ctcgccgtcg ggcttgctcg ccttgagcct ggcgaacagt tcggctggcg
     4261 cgagcccctg atgctcttga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     4321 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     4381 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     4441 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     4501 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     4561 cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     4621 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     4681 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     4741 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc gatctttgca aaagcctagg
     4801 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgagga ggcggcctcg
     4861 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
     4921 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
     4981 tgcttgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct
     5041 gactaattga gatgcttgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     5101 caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc ttcctttttc
     5161 aataaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5521 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5821 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6181 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6241 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6301 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6361 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6421 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6481 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6541 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6601 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacg
//