LOCUS       pmax_GFP_C289T.xdna    3526 bp      DNA     circular   7/18/17
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pmaxcloning.xdna [2775  nt] : (SacI[1043] / Kp
            nI[947]) to GFP_PCR_Product_for_Pmax [743  nt] : (KpnI[5] / 
            SacI[752])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1..798
                     /label=PCMV IE
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= immediate early promoter of cytomegalovirus (PCMV IE)  \ 
     misc_feature    811..947
                     /label=Chimeric intron
                     /SerialCloner_Color=&hB3B3B3
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
                     /SerialCloner_Desc=chimeric intron for enhanced gene expression \ 
     misc_feature    1702..1902
                     /label=SV40 poly A
                     /SerialCloner_Color=&hB3B3B3
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
                     /SerialCloner_Desc=SV40 late mRNA polyadenylation signal \ 
     misc_feature    1976..2617
                     /label=pUC ori
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
                     /SerialCloner_Desc=pUC plasmid replication origin \ 
     misc_feature    complement(2679..3470)
                     /label=KANr
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Kanamycin resistance gene \ 
     misc_feature    complement(3471..3502)
                     /label=P
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Bacterial promoter for expression of Kanr gene \ 
                     952..1255
                     /label=insert from GFP_PCR_Product_for_Pmax [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from GFP_PCR_Product_for_Pmax [743  nt] : (KpnI[5] / SacI[752]) \ 
                     1256..1256
                     /label=insert from GFP_PCR_Product_for_Pmax [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from GFP_PCR_Product_for_Pmax [743  nt] : (KpnI[5] / SacI[752]) \ 
                     1258..1694
                     /label=insert from GFP_PCR_Product_for_Pmax [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from GFP_PCR_Product_for_Pmax [743  nt] : (KpnI[5] / SacI[752]) \ 
     misc_feature    968..1687
                     /label=GFP [*]
                     /SerialCloner_Color=&h08C019
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1570..1576
                     /label=5'Nb.Bpu10I
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1588..1594
                     /label=3'Nb.Bpu10I
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1256..1256
                     /label=C289T
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg 
      721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat 
      781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt 
      841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg 
      901 cacctattgg tcttactgac atccactttg cctttctctc cacaggggta cCGCTAGCGC 
      961 TGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA 
     1021 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC 
     1081 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG 
     1141 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA 
     1201 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGTGCAC 
     1261 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA 
     1321 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT 
     1381 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA 
     1441 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA 
     1501 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA 
     1561 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA 
     1621 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA 
     1681 CAAGTGAAAG CTTGagctcg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa 
     1741 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa 
     1801 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg 
     1861 ggaggttttt taaagcaagt aaaacctcta caaatgtggt acttaagagg gggagaccaa 
     1921 agggcgagac gttaaggcct cacgtgacat gtgagcaaaa ggccagcaaa aggccaggaa 
     1981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     2041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     2101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     2161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     2221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     2281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     2341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     2401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     2461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     2521 caaacaaacc acgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 
     2581 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 
     2641 gaaaactcac gttaagggat tttggtcatg ccgtctcaga agaactcgtc aagaaggcga 
     2701 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca 
     2761 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag 
     2821 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc 
     2881 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg 
     2941 ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga 
     3001 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc 
     3061 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca 
     3121 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc 
     3181 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg 
     3241 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc 
     3301 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg 
     3361 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc 
     3421 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat aatattattg 
     3481 aagcatttat cagggttcgt ctcgtcccgg tctcctccca tgcatg
//