LOCUS       Exported                4469 bp DNA     circular SYN 07-OCT-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   john
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..250
                     /label=TBP-proximal-promoter
                     /note="color: #993366; direction: RIGHT"
     regulatory      277..286
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             283..1935
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /note="color: #ffcc00"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1979..2100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(1990..2009)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     rep_origin      complement(2519..3107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(3307..4167)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3307 .. 4098 / #ccffcc
                        2: 4099 .. 4167 / #ccffcc / signal sequence
                     Cleavage site after base 4099"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4272..4320
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    4334..4425
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
                     /note="color: #a6acb3"
     misc_feature    4334..4407
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
                     /note="color: #a6acb3"
     primer_bind     4374..4393
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
                     /note="color: black; sequence: CTAGCAAAATAGGCTGTCCC; added:
                     2019-04-20"
ORIGIN
        1 ctacacagag aaactctttc tcacaaaaac aaatcatgga agactgagga tgtagttcag
       61 tggtagaacg cttgcttggg cttgatcgcc attgccacat ccccaactgt ttccgctaca
      121 cactcaatag atctctcttt gacagctaga aagggccgtc ggaggccccg ccccggaact
      181 gctggtaccc ggcccgctcg tttccttccc agtgtctcac agcttaagca caacaaatcg
      241 gactgcagtc cttggcaatc cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac
      301 attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg
      361 cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat
      421 atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct
      481 atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg
      541 cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac
      601 gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta
      661 ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata
      721 caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc
      781 ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc
      841 gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag
      901 ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc
      961 ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt tcaccacggc
     1021 ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac
     1081 cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg
     1141 ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta
     1201 agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc
     1261 gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc
     1321 agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg
     1381 cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag
     1441 cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag
     1501 gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg
     1561 gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc
     1621 taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac
     1681 gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg
     1741 ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt
     1801 acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg
     1861 accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc
     1921 aagatcgccg tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     1981 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2041 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2101 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2161 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc
     2221 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     2281 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     2341 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2401 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2461 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2701 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2941 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3241 caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg
     3301 cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc
     3361 gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc
     3421 aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc
     3481 aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc
     3541 catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt
     3601 ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc
     3661 ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag
     3721 aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt
     3781 gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg
     3841 cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc
     3901 aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa
     3961 agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt
     4021 gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt
     4081 caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag
     4141 tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta
     4201 tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt
     4261 ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     4321 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
     4381 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctggcct aactggccgg
     4441 tacctgagct cgctagcctc gaggatatc
//
