LOCUS       Exported                5612 bp DNA     circular SYN 07-OCT-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   john
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    21..270
                     /label=TBP-proximal-promoter
                     /note="color: #993366; direction: RIGHT"
     regulatory      297..306
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             303..1955
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /note="color: #ffcc00"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1999..2120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(2010..2029)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     promoter        2229..3355
                     /label=Chantico Promoter
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    3043..3050
                     /label=TBX5consensus
                     /note="color: #000000"
     rep_origin      complement(3662..4250)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(4450..5310)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4450 .. 5241 / #ccffcc
                        2: 5242 .. 5310 / #ccffcc / signal sequence
                     Cleavage site after base 5243"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5415..5463
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    5477..5568
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
                     /note="color: #a6acb3"
     misc_feature    5477..5550
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
                     /note="color: #a6acb3"
     primer_bind     5517..5536
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
                     /note="color: black; sequence: CTAGCAAAATAGGCTGTCCC; added:
                     2019-04-20"
ORIGIN
        1 tcgctagcct cgaggatatc ctacacagag aaactctttc tcacaaaaac aaatcatgga
       61 agactgagga tgtagttcag tggtagaacg cttgcttggg cttgatcgcc attgccacat
      121 ccccaactgt ttccgctaca cactcaatag atctctcttt gacagctaga aagggccgtc
      181 ggaggccccg ccccggaact gctggtaccc ggcccgctcg tttccttccc agtgtctcac
      241 agcttaagca caacaaatcg gactgcagtc cttggcaatc cggtactgtt ggtaaagcca
      301 ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg
      361 ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca
      421 tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga
      481 gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg
      541 tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg
      601 tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca
      661 tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc
      721 aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg
      781 gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg
      841 acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg
      901 gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca
      961 gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg
     1021 tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct
     1081 ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact
     1141 ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc
     1201 tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca
     1261 gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct
     1321 acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg
     1381 gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta
     1441 agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg
     1501 gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca
     1561 gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga
     1621 gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc
     1681 aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc
     1741 tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg
     1801 actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg
     1861 acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca
     1921 ttaaggccaa gaagggcggc aagatcgccg tgtaataatt ctagagtcgg ggcggccggc
     1981 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     2041 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     2101 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     2161 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
     2221 aggatccggt cattcagtaa atataaatgc agatagccta cgttctgaaa gtggggttct
     2281 agggtccctt ggttagtttt aaaaattcaa agtcactttg agttttgttt tgttttgttt
     2341 tgttttaaag cttgagagtg gccgtgcaga gcaaagttgt ccaattaagt ttatatcagt
     2401 gacagatggg cttatcagac cttcgctctc agacagtagc catggaacac ttagctgctg
     2461 cctcccgaaa gtgcaagcag accagttcag gatctgaagc tttgttttgg tttgacttaa
     2521 atgtaagtgc aaacagtcgc tgtggggtgt gtgtgtgtgt gtgtgtgtga tactggccga
     2581 tgcaggctga gagtaggggt gtctcactcc tgactgcgta ctccccctct gttttacaca
     2641 ggcttattta ctgttacacg gagacttttc ttcgaactgt taaactcttg gctgagtttg
     2701 tgtggagtat gaggcaaatc tctaaccgca agcatgcagt ggaaagatta ggcatacttt
     2761 tgaaacgagt ttgtgtacca gatcatattc ttggctcaag aaagaaaaag aaagggggct
     2821 ggggaaaggc ttgtggttgc tactctggga actaaagaat tcaaaaggct tgttctccat
     2881 gtttcgtaga cacagacatc tggaattgtt gttttaaaat tacaagcaat tgtgtatgct
     2941 ttgttactgc aacacttatt cagaggcaag gacataggtg ccactgttgt cagaaagcca
     3001 gatgtccttt ccttaaattg gcctgcctgg ctgctggcgc ctgggtgtca gcagacactt
     3061 tgggaatctc agttagcggt actgcatgga tatagtgcat ctgcttctca cacttctgcc
     3121 aaccttgtgt attggtttgc ttctctccat ggtgttaaaa caatgactga aagcgtcttg
     3181 ggtagggcaa gggtatattt agcttatagt tgcacatgac agtccatcgt caagggagag
     3241 catggcggga actcaaggac tttgaggagg aactgaagca caggcataga ggctgttcct
     3301 cactggcttg ctccccaggg cttgctcagc tagctgtctt acagagccca gaaccccgat
     3361 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     3421 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     3481 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3541 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3601 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3661 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3721 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3781 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3841 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3901 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3961 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4021 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4081 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4141 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4201 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4261 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4321 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4381 aatcaatcta aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca
     4441 ctgcagtggt taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg
     4501 ttcgtccata gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc
     4561 atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc
     4621 agcaatgaac cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc
     4681 ctccatccag tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag
     4741 tttccgaaga gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat
     4801 ggcttcgttc aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg
     4861 aagaaatgca gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt
     4921 gttgtcgctc atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag
     4981 atgcttttcc gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg
     5041 accaagctgc tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt
     5101 gaaagtgctc atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct
     5161 attgagatcc agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac
     5221 tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat
     5281 gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat
     5341 ttatcagggt tactagtacg tctctcaagg ataagtaagt aatattaagg tacgggaggt
     5401 attggacagg ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     5461 gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag
     5521 caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctgg cctaactggc
     5581 cggtacctga gctcgctagc ctcgaggata tc
//
