LOCUS       ptia1l-hsp-GFP          6637 bp ds-DNA     circular     08-OCT-2021
DEFINITION  .
ACCESSION   urn.local...h-dya7qgj
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6344..3637
                     /standard_name="TOPO backbone"
     rep_origin      complement(164..846)
     CDS             complement(944..1603)
                     /standard_name="AmpR"
     rep_origin      complement(3062..3368)
     CDS             complement(3386..3454)
                     /standard_name="LacZ alpha"
     primer_bind     3525..3542
                     /standard_name="M13-fwd"
     primer_bind     3541..3568
                     /standard_name="T7"
     misc_feature    3638..3666
                     /standard_name="Tia1l CRISPR target"
     misc_feature    3829..5343
                     /standard_name="hsp70 minimal promoter"
     misc_feature    4338..4345
                     /standard_name="(Weaker) TATA box"
     misc_feature    4765..4772
                     /standard_name="TATA box"
     misc_feature    4795..4900
                     /standard_name="hsp70 clear UTR"
     misc_feature    5363..6079
                     /standard_name="eGFP"
     polyA_signal    6102..6293
     primer_bind     complement(6413..6433)
                     /standard_name="M13-rev"
     misc_binding    complement(6439..6461)
     promoter        complement(6465..6495)
                     /standard_name="lac promoter"
ORIGIN      
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaattc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     1861 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     1921 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     1981 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     2041 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     2101 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     2161 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     2221 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     2281 ctcggcagga gcaaggtggg atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     2341 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     2401 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     2461 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     2521 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     2581 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     2641 atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     2701 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     2761 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     2821 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     2881 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     2941 ccctgaattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3001 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3061 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3121 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3181 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3241 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3301 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3361 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     3421 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3481 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3541 gtgaattgta atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagcg
     3601 gccgccagtg tgatggatat ctgcagaatt cgcccttgaa ttcggtatgt cgggaacctc
     3661 tccagggaaa agcctctcac aggcagccga tgcgggaaga ggtgtattag tcttgataga
     3721 gaggctgcaa atagcaggaa acgtgagcag agactccctg gtgtctgaaa cacaggccag
     3781 ataggcccct tacgtacgct agcgggcccg atatcaccgg gggatccttc aggggtgtcg
     3841 cttggttatt tccaaaaatc aaattaattt tattaaacta ttagaacgag catgttttgt
     3901 ctatatgcta cagaagataa aaaataatag gagttaacag ttataaaaca acacactttg
     3961 tttctattga ttgttgacca cactggggtc tcattaagtt agattaaaga cacactaact
     4021 gggtcaaaag cagcagattg atttcatagc accagggtaa actttctaac acttttacgg
     4081 caatcatata cattaaaatt aaatacagac cacgactgaa caaggaggat gatctccaat
     4141 attaaacaaa gggacttgtg cctatttctc tgagggtaaa catgacctct caagttagca
     4201 agttgttttt aacactacaa aaatagttaa gactgcaatc ccagaataaa gtattggttt
     4261 taaccaatca atatagtaca gtaaacatcc atttgttttg ttgaaacgtt aaacaaatct
     4321 gaccaaagct attagcttat ataaaacagg tttgccttct atgtagctga aaacaccaca
     4381 ggcccgattt tgctactgtg taaaacattt cagcaagatt tttttattgc attttttttt
     4441 actgaatcgt tcaaacattt tatcatttta gtttgttcat tcattgcaac tggaaaaaca
     4501 acacatcaca caaccgcaca tatttcagca ataagtacaa taaaacactc aaataaaaaa
     4561 aacattttaa atctctttgt atttttgacc gctgtttcgc gtaatttcac ggtaaaactc
     4621 tggaaatctc cactacattc ctctcagcgg ctcctctcaa tgacagctga agaagtgacg
     4681 cggctgcctg ctgtgttttg attggtcgaa ttcactggag gcttccagaa cagtgtagag
     4741 tctgaacggg tgcgcgctct gctgtattta aagggcgaaa gagagaccgc agagaaactc
     4801 aaccgaagag aagcgacttg acaaagaaga aaagagcagc ctgacaggac tttttccccg
     4861 acgaggtgtt tattcgctct atttaagaat ctactgtaag gtaagtctca atatattgta
     4921 ctctattggc taatcagaat tatatagaga ttatatgtac ttaatgtcaa aaaatcaact
     4981 ttgtatatgt aatcttttta catgtggact gcctatgttc atcttatttt aggtctacta
     5041 gaaaattata tttcccgttt tcacaataag gatttttaaa aaaagcaatg aacagacggg
     5101 catttacttt atgttgctga cattatttta catgagcata ataaccataa atactagcaa
     5161 atgtcctaaa tgaatttgtg ttaatgttgt ctacaaaaga aaattagcgt tttacttgta
     5221 caactaataa taacttggtt attaagagaa tttcacttgt tgactagaaa aatcctttca
     5281 taatgaaaca attgcaccat aaattgtata aatataaaat taattctaat tgtttttttt
     5341 cctgcagtcg acggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     5401 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     5461 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5521 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     5581 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5641 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5701 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5761 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     5821 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     5881 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5941 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     6001 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     6061 catggacgag ctgtacaagt aaagcgacat cgatgatgat ccagacatga taagatacat
     6121 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     6181 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     6241 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     6301 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctaaagggcg aattccagca
     6361 cactggcggc cgttactagt ggatccgagc tcggtaccaa gcttggcgta atcatggtca
     6421 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6481 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6541 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6601 caacgcgcgg ggagaggcgg tttgcgtatt gggcgct
//
