LOCUS       Exported                6234 bp DNA     circular SYN 17-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Oct 17, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1035..1365)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1049..1184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1398..1638
                     /label=U6 Promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    1647..1673
                     /label=27
     misc_feature    1680..1762
                     /label=Squash
     misc_feature    1764..1789
                     /label=Terminator stem-loop
                     /label=5S(1)
     misc_feature    1764..1788
                     /label=terminator
     polyA_signal    1873..1994
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2303..2731)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(2408..2714)
                     /direction=LEFT
                     /label=F1 ori
     promoter        2758..2862
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2863..3723
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             3061..3720
                     /label=AmpR
     polyA_signal    4925..5059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4929..5120
                     /label=SV40 late polyA
     polyA_signal    4938..5059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5484..5504)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5634..5699
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
ORIGIN
        1 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
       61 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
      121 gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct
      181 ggcgcgagcc cctgatgctc ttgtccagat catcctgatc gacaagaccg gcttccatcc
      241 gagtacgtgc tcgctcgatg cgatgttcgc ttggtggtcg aatgggcagg tagccggatc
      301 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
      361 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
      421 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
      481 ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
      541 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
      601 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
      661 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc
      721 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
      781 aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc
      841 ccagtctagc tatcggcatg taagcccact gcaagctacc tgctttctct ttgcgcttgc
      901 gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg
      961 cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg
     1021 gcacggtgaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     1081 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     1141 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     1201 gggactatgg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     1261 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     1321 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagccggat
     1381 ccaaggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat
     1441 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac
     1501 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     1561 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     1621 atatcttgtg gaaaggacga aacaccgtgc tcgcttcggc agcacatata ctagtcgacc
     1681 ctaggctaca aggtgagccc aataatacgg tttgggttag gataggaagt agagccgtaa
     1741 actctctaag cggtagtcta ggtctagagc ggacttcggt ccgcttttta ctaggacctg
     1801 caggcatgca agcttgacgt cggttaccga tatccatatg gcggccgcat cgatctcgag
     1861 ccgcggacta gtaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1921 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1981 atcaatgtat cttatcatgt cttacgtaga taagtagcat ggcgggttaa tcattaacta
     2041 caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     2101 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     2161 gcgagcgcgc agagagggag tggccaaaga tctctggcgt aatagcgaag aggcccgcac
     2221 cgatcgccct tcccaacagt tgcgcagcct gaatggctaa tgggaaattg taaacgttaa
     2281 tattttgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     2341 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     2401 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     2461 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     2521 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gatgccccga
     2581 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     2641 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     2701 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     2761 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2821 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2881 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2941 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3001 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3061 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3121 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3181 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3241 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3301 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3361 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3421 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3481 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3541 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3601 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3661 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3721 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3781 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3841 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3901 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3961 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4021 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4081 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4141 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4201 cggtcgggct gaacgggggg ttcgtgcaac acagccagct tggagcgaac gacctacacc
     4261 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     4321 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4381 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4441 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4501 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4561 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4621 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     4681 ccgcctctcc ccgcgcgttg gccgattcat taatgcagag atctttggcc actccctctc
     4741 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4801 cccgggcggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta
     4861 ggggttcctg gaggggtgga gtcgtgacgt gaattacgtc atagggttag ggaggtcctg
     4921 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     4981 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     5041 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     5101 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     5161 tgatctctag tcaaggcact atacatcaaa tattccttat taaccccttt acaaattaaa
     5221 aagctaaagg tacacaattt ttgagcatag ttattaatag cagacactct atgcctgtgt
     5281 ggagtaagaa aaaacagtat gttatgatta taactgttat gcctacttat aaaggttaca
     5341 gaatattttt ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata
     5401 gcaaagcaag caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct
     5461 gaaggaaagt ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga
     5521 gagtcagcag tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc
     5581 tcattaaagg cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat
     5641 acacaaacaa ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc
     5701 ttagagcttt aaatctctgt aggtagtttg tccaattatg tcacaccaca gaagtaaggt
     5761 tccttcacaa agatccggga ccaaagcggc catcgtgcct ccccactcct gcagttcggg
     5821 ggcatggatg cgcggatagc cgctgctggt ttcctggatg ccgacggatt tgcactgccg
     5881 gtagaactcc gcgaggtcgt ccagcctcag gcagcagctg aaccaactcg cgaggggatc
     5941 gagcccgggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
     6001 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
     6061 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     6121 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     6181 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aata
//
