LOCUS       p2B55                   7592 bp    DNA     circular UNA 31-AUG-2021
DEFINITION  Insertion of pTDH3-rtTA-tPGK1 by Gibson Assembly in pRS426 backbone
            digested with HindIII/EcoRI.
ACCESSION   urn.local...37-dfzgc5y
VERSION     urn.local...37-dfzgc5y
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        190..415
     misc_feature    417..1217
                     /note="Geneious type: marker"
     misc_feature    complement(1483..1789)
                     /note="Geneious type: origin of replication"
     misc_feature    complement(1810..1970)
                     /note="Geneious type: reporter"
     primer_bind     1975..1994
     promoter        1977..1995
     misc_feature    2016..2040
     primer_bind     2016..2067
     misc_feature    2016..2045
                     /note="Geneious type: Editing History Insertion"
     source          <2046..>2712
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        2046..2712
                     /gene="S. cerevisiae TDH3"
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase"
     misc_feature    2224
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(2691..2737)
     primer_bind     complement(2691..2712)
     CDS             2713..3726
     misc_feature    2713..2737
     primer_bind     2713..3776
     misc_feature    2713..2737
                     /note="Geneious type: Editing History Insertion"
     primer_bind     2789..2808
     primer_bind     complement(2983..3002)
     primer_bind     3566..3585
     terminator      3727..3917
     misc_feature    3748..3776
     primer_bind     3748..3947
     primer_bind     complement(3748..3947)
     misc_feature    3923..3947
     promoter        complement(3989..4008)
     primer_bind     complement(3990..4009)
     misc_feature    complement(4047..4074)
                     /note="Geneious type: regulatory sequence"
     promoter        complement(4078..4107)
     misc_feature    complement(4416..5035)
                     /note="Geneious type: origin of replication"
     misc_feature    complement(5190..6050)
                     /note="Geneious type: marker"
     promoter        complement(6092..6120)
     misc_feature    complement(6182..7342)
                     /note="Geneious type: origin of replication"
     gene            complement(7145..7192)
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
     2041 agctttcatt atcaatactg ccatttcaaa gaatacgtaa ataattaata gtagtgattt
     2101 tcctaacttt atttagtcaa aaaattagcc ttttaattct gctgtaaccc gtacatgccc
     2161 aaaatagggg gcgggttaca cagaatatat aacatcgtag gtgtctgggt gaacagttta
     2221 ttcctggcat ccactaaata taatggagcc cgctttttaa gctggcatcc agaaaaaaaa
     2281 agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag gtccattctc
     2341 ttagcgcaac tacagagaac aggggcacaa acaggcaaaa aacgggcaca acctcaatgg
     2401 agtgatgcaa cctgcctgga gtaaatgatg acacaaggca attgacccac gcatgtatct
     2461 atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa aaagctgaaa
     2521 aaaaaggttg aaaccagttc cctgaaatta ttcccctact tgactaataa gtatataaag
     2581 acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa attctacttt
     2641 tatagttagt ctttttttta gttttaaaac accaagaact tagtttcgaa taaacacaca
     2701 taaacaaaca aaatgtctag attagataaa agtaaaatta ttaacggcgc attagagctg
     2761 cttaatgggg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta
     2821 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttaccc
     2881 attgagatgt tagataggca ccatactcac tcttgccctt tagaacctga aagctggcaa
     2941 gattttttac gtaataacgc taaaagttat agatgtgctt tactaagtca tcgcgatgga
     3001 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa
     3061 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct
     3121 gtggggcact ttactttagg ttgcgtattg gaagaacaag agcatcaagt cgctaaagaa
     3181 gaaagggaaa cacctactac tgatagtatg ccgccattat taaaacaagc tatcgaatta
     3241 tttgatcgcc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga
     3301 ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca gccgcgcgcg tacgaaaaac
     3361 aattacgggt ctaccatcga gggcctgctc gatctcccgg acgacgacgc ccccgaagag
     3421 gcggggctgg cggctccgcg cctgtccttt ctccccgcgg gacacacgcg cagactgtcg
     3481 acggcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg cgaggacgtg
     3541 gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg ggacggggat
     3601 tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct ggatatggcc
     3661 gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta cggtgggact
     3721 agataaattg aattgaattg aaatcgatag atcaattttt ttcttttctc tttccccatc
     3781 ctttacgcta aaataatagt ttattttatt ttttgaatat tttttattta tatacgtata
     3841 tatagactat tatttatctt ttaatgatta ttaagatttt tattaaaaaa aaattcgctc
     3901 ctcttttaat gcctttagaa ttcctgcagc ccgggggatc cactagttct agagcggccg
     3961 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     4021 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4081 ataggagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca
     4141 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     4201 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4261 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4321 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4381 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4441 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4501 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4561 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4621 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4681 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4741 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4801 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4861 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4921 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4981 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5041 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5101 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5161 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5221 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5281 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5341 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5401 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5461 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5521 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5581 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5641 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5701 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5761 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5821 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5881 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5941 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6001 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6061 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6121 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6181 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
     6241 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
     6301 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
     6361 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
     6421 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
     6481 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
     6541 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
     6601 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
     6661 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
     6721 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
     6781 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
     6841 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
     6901 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
     6961 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
     7021 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
     7081 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
     7141 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
     7201 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
     7261 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
     7321 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
     7381 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
     7441 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
     7501 tatcatttcc tttgatattg gatcatacta agaaaccatt attatcatga cattaaccta
     7561 taaaaatagg cgtatcacga ggccctttcg tc
//
