LOCUS       p2B56                   7425 bp    DNA     circular UNA 11-JUN-2021
DEFINITION  Insertion of pMYO2-rtTA-tPGK1 by Gibson Assembly in pRS426 backbone
            digested with HindIII/EcoRI.
ACCESSION   urn.local...40-dfzhfjz
VERSION     urn.local...40-dfzhfjz
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        190..415
     misc_feature    417..1217
                     /note="Geneious type: marker"
     misc_feature    complement(1483..1789)
                     /note="Geneious type: origin of replication"
     misc_feature    complement(1810..1970)
                     /note="Geneious type: reporter"
     primer_bind     1975..1994
     promoter        1977..1995
     misc_feature    2016..2040
     primer_bind     2016..2066
     source          <2046..>2545
                     /organism="Saccharomyces cerevisiae S288c"
                     /mol_type="genomic DNA"
                     /strain="S288c"
                     /db_xref="taxon:559292"
                     /chromosome="XV"
     promoter        2046..2545
     primer_bind     complement(2525..2570)
     CDS             2546..3559
     misc_feature    2546..2570
     primer_bind     2546..3609
     primer_bind     2622..2641
     primer_bind     complement(2816..2835)
     primer_bind     3399..3418
     terminator      3560..3750
     misc_feature    3581..3609
     primer_bind     3581..3780
     primer_bind     complement(3581..3780)
     misc_feature    3756..3780
     promoter        complement(3822..3841)
     primer_bind     complement(3823..3842)
     misc_feature    complement(3880..3907)
                     /note="Geneious type: regulatory sequence"
     promoter        complement(3911..3940)
     misc_feature    complement(4249..4868)
                     /note="Geneious type: origin of replication"
     misc_feature    complement(5023..5883)
                     /note="Geneious type: marker"
     promoter        complement(5925..5953)
     misc_feature    complement(6015..7175)
                     /note="Geneious type: origin of replication"
     gene            complement(6978..7025)
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
     2041 agcttccgtt tctcgatgct tatctggttt agtttacgct gttaaaacca aaaccccaac
     2101 agattttcga ccctaacgta tgtagggcta aaatagatat tgagtaggtt acaattaatt
     2161 attggcaatt gcacctagtg acacatttac gaaaacgtag ggcaaaaact attacccgac
     2221 ccagggctat tttgtgattt tttccttttt tttgtttatg atcgcgcttc tcgaaaagcc
     2281 aaatatcaga aatcccaaac acgccttcat ttgatacgat tcgtagcctg cgtttcagag
     2341 atctatcaac tttgcaaggc caatcagaga acaaaaaagt ctcgcaaagt catttcactt
     2401 ttctcgcttg aaattattcg ttcgatttct ggctgcttgc ttgttttttg ttttctaagg
     2461 tactattcga caccattcca ttggacagcg atacttatac cattgtacat ataggacata
     2521 aaaacagcag atattacagc gtataatgtc tagattagat aaaagtaaaa ttattaacgg
     2581 cgcattagag ctgcttaatg gggtcggaat cgaaggttta acaacccgta aactcgccca
     2641 gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct
     2701 cgacgcctta cccattgaga tgttagatag gcaccatact cactcttgcc ctttagaacc
     2761 tgaaagctgg caagattttt tacgtaataa cgctaaaagt tatagatgtg ctttactaag
     2821 tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac
     2881 tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata
     2941 tgcactcagc gctgtggggc actttacttt aggttgcgta ttggaagaac aagagcatca
     3001 agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat tattaaaaca
     3061 agctatcgaa ttatttgatc gccaaggtgc agagccagcc ttcttattcg gccttgaatt
     3121 gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgcgt acagccgcgc
     3181 gcgtacgaaa aacaattacg ggtctaccat cgagggcctg ctcgatctcc cggacgacga
     3241 cgcccccgaa gaggcggggc tggcggctcc gcgcctgtcc tttctccccg cgggacacac
     3301 gcgcagactg tcgacggccc ccccgaccga tgtcagcctg ggggacgagc tccacttaga
     3361 cggcgaggac gtggcgatgg cgcatgccga cgcgctagac gatttcgatc tggacatgtt
     3421 gggggacggg gattccccgg gtccgggatt taccccccac gactccgccc cctacggcgc
     3481 tctggatatg gccgacttcg agtttgagca gatgtttacc gatgcccttg gaattgacga
     3541 gtacggtggg actagataaa ttgaattgaa ttgaaatcga tagatcaatt tttttctttt
     3601 ctctttcccc atcctttacg ctaaaataat agtttatttt attttttgaa tattttttat
     3661 ttatatacgt atatatagac tattatttat cttttaatga ttattaagat ttttattaaa
     3721 aaaaaattcg ctcctctttt aatgccttta gaattcctgc agcccggggg atccactagt
     3781 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
     3841 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3901 aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3961 gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4021 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4081 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4141 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4681 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4981 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5041 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5101 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5161 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5221 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5281 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5341 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5401 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5461 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5521 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5581 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5641 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5701 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5761 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5821 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5881 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5941 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6001 aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga
     6061 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     6121 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa
     6181 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     6241 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa
     6301 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg
     6361 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa
     6421 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg
     6481 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta
     6541 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat
     6601 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat
     6661 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa
     6721 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag
     6781 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat
     6841 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt
     6901 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg
     6961 gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
     7021 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac
     7081 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga
     7141 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg
     7201 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc
     7261 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct
     7321 catccttcaa tgctatcatt tcctttgata ttggatcata ctaagaaacc attattatca
     7381 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
