LOCUS       p2B57                   7025 bp    DNA     circular UNA 11-JUN-2021
DEFINITION  Insertion of pTET3-eGFP-tADH1 by Gibson Assembly in pRS426 backbone
            digested with HindIII/EcoRI.
ACCESSION   urn.local...5q-dfzlt32
VERSION     urn.local...5q-dfzlt32
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        190..415
     misc_feature    417..1217
                     /note="Geneious type: marker"
     misc_feature    complement(1483..1789)
                     /note="Geneious type: origin of replication"
     misc_feature    complement(1810..1970)
                     /note="Geneious type: reporter"
     primer_bind     1975..1994
     promoter        1977..1995
     misc_feature    2016..2040
     primer_bind     2016..2408
     promoter        2046..2408
     primer_bind     2046..2070
     protein_bind    2046..2064
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2112..2130
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(2183..2202)
     protein_bind    2196..2214
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2366..2385
     primer_bind     complement(2382..2408)
     misc_feature    2384..2425
                     /note="Geneious type: Editing History Insertion"
     misc_feature    2384..2408
     primer_bind     2384..2428
     CDS             2426..3142
     source          <2426..>3350
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(2659..2678)
     primer_bind     3011..3030
     misc_feature    3150..3162
     terminator      3163..3350
                     /gene="S. cerevisiae ADH1"
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3328..3380)
     misc_feature    3351..3380
                     /note="Geneious type: Editing History Insertion"
     misc_feature    3356..3380
     promoter        complement(3422..3441)
     primer_bind     complement(3423..3442)
     misc_feature    complement(3480..3507)
                     /note="Geneious type: regulatory sequence"
     promoter        complement(3511..3540)
     misc_feature    complement(3849..4468)
                     /note="Geneious type: origin of replication"
     misc_feature    complement(4623..5483)
                     /note="Geneious type: marker"
     promoter        complement(5525..5553)
     misc_feature    complement(5615..6775)
                     /note="Geneious type: origin of replication"
     gene            complement(6578..6625)
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
     2041 agctttccct atcagtgata gagatgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg
     2101 cagatgtgcc ttccctatca gtgatagaga aacaataaag attctacaat actagctttt
     2161 atggttatga agaggaaaaa ttggcagtaa cctggtccct atcagtgata gagataatta
     2221 atcagcgaag cgatgatttt tgatctatta acagatatat aaatggaaaa gctgcataac
     2281 cactttaact aatactttca acattttcag tttgtattac ttcttattca aatgtcataa
     2341 aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga gaaaaaacta
     2401 taactagagt gctggtttaa ttaacatgtc aaaaggcgag gaactattca caggtgtcgt
     2461 accaatactg gtagaattgg atggcgacgt aaacgggcat aagttttctg tgagtggtga
     2521 gggtgaaggg gacgcaactt atggcaaact tacacttaag ttcatctgca ccacagggaa
     2581 gctgcctgtc ccatggccaa ctctagttac aactctcact tacggagtac aatgtttttc
     2641 tcgataccca gaccacatga aacaacatga ctttttcaaa tctgccatgc ctgagggata
     2701 tgtgcaagag agaactattt tctttaaaga tgatggtaat tacaaaactc gtgctgaagt
     2761 gaagtttgaa ggagatacac ttgttaatag aattgagtta aagggtattg actttaagga
     2821 agatggtaat atcttaggtc acaaattgga atacaactac aattcacata acgtttacat
     2881 catggcagat aaacagaaaa acggcataaa agttaatttc aaaattagac ataatatcga
     2941 agatggttcc gttcaactcg ctgatcatta tcaacaaaat acaccaatcg gagatggacc
     3001 tgttctattg ccagacaacc attacttatc tacacagagc gccttgtcaa aagatcctaa
     3061 tgaaaagaga gatcacatgg ttttgttaga atttgtcacc gctgcgggta taacgttggg
     3121 catggatgaa ttatacaagt aaggcgcgcc acttctaaat aagcgaattt cttatgattt
     3181 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac
     3241 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg
     3301 ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg gaattcctgc
     3361 agcccggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
     3421 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     3481 tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa
     3541 gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct
     3601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3661 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3721 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3781 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4021 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4081 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4141 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4201 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4261 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     4321 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4381 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4441 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4501 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4561 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4621 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4681 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4741 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4801 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4861 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4921 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4981 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5041 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5101 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5161 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5221 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5281 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5341 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5401 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5461 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5521 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5581 gttccgcgca catttccccg aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt
     5641 agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
     5701 tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt
     5761 tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     5821 atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact
     5881 tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag
     5941 attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg
     6001 taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa
     6061 gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa
     6121 gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag
     6181 aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt
     6241 tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct
     6301 atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa
     6361 aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata
     6421 tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc
     6481 ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt
     6541 gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga
     6601 ataggaactt cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc
     6661 aacgcgagct gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct
     6721 gtatatatat atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta
     6781 tatgcgtcta tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt
     6841 ccatgcgggg tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca
     6901 ctcctcaatt ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata
     6961 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     7021 tcgtc
//
