LOCUS       Exported                8816 bp DNA     circular SYN 02-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Doudna Lab
  TITLE     Direct Submission
  JOURNAL   Exported Oct 13, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2372
                     /organism="Severe acute respiratory syndrome coronavirus
                     2"
                     /host="Homo sapiens"
                     /isolate="Wuhan-Hu-1"
                     /mol_type="genomic RNA"
                     /country="China"
                     /collection_date=Dec-2019
                     /db_xref="taxon:2697049"
     source          2373..3464
                     /organism="Severe acute respiratory syndrome coronavirus
                     2"
                     /host="Homo sapiens"
                     /isolate="Wuhan-Hu-1"
                     /mol_type="genomic RNA"
                     /country="China"
                     /collection_date=Dec-2019
                     /db_xref="taxon:2697049"
     enhancer        3..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        383..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     404..423
                     /label=CMV2-F
     primer_bind     537..557
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     599..618
                     /label=P675
     primer_bind     631..650
                     /label=T7
                     /note="T7 promoter, forward primer"
     primer_bind     657..686
                     /label=B7-NheI-C-Luc-F
     primer_bind     669..686
                     /label=C-Luc-F
     CDS             675..2327
                     /codon_start=1
                     /locus_tag="Luc2"
                     /label=Luc2
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     2276..2299
                     /label=Luc2-seq-R
     primer_bind     complement(2310..2333)
                     /label=B8-EcoRI-C-Luc-R
     primer_bind     complement(2310..2327)
                     /label=C-Luc2-R
     primer_bind     complement(2333..2369)
                     /label=SDM-LIC-R
     primer_bind     2352..2392
                     /label=LIC-F-CVF20
     primer_bind     2352..2373
                     /label=LIC-F-CVF21
     primer_bind     2352..2373
                     /label=PPS1-F
     primer_bind     2352..2371
                     /label=partial-LIC-F
     gene            2372
                     /gene="orf1ab"
                     /locus_tag="GU280_gp01"
                     /label=orf1ab
                     /db_xref="GeneID:43740578"
     gene            2373..3464
                     /gene="orf1ab"
                     /locus_tag="GU280_gp01"
                     /label=orf1ab
                     /db_xref="GeneID:43740578"
     misc_feature    2373..3464
                     /label=PS9
     mat_peptide     2373..2951
                     /gene="orf1ab"
                     /locus_tag="GU280_gp01"
                     /product="endoRNAse"
                     /label=nsp15
                     /note="nsp15-A1 and nsp15B-NendoU; produced by pp1ab only"
                     /note="/protein_id=YP_009725310.1"
     primer_bind     2947..2970
                     /label=PPS1-F
     mat_peptide     2952..3464
                     /gene="orf1ab"
                     /locus_tag="GU280_gp01"
                     /product="2'-O-ribose methyltransferase"
                     /label=nsp16
                     /note="nsp16_OMT; 2'-o-MT; produced by pp1ab only"
                     /note="/protein_id=YP_009725311.1"
     primer_bind     3205..3225
                     /label=LIC-F-CVF21
     primer_bind     complement(3229..3248)
                     /label=B6-LIC-R-CVR19
     primer_bind     complement(3229..3247)
                     /label=LIC-R-CVR19
     primer_bind     complement(3441..3480)
                     /label=PPS1-R
     primer_bind     complement(3464..3480)
                     /label=LIC-R-CVR19
     primer_bind     complement(3464..3480)
                     /label=B6-LIC-R-CVR19
     primer_bind     3466..3509
                     /label=SDM-LIC-F
     primer_bind     complement(3466..3480)
                     /label=Partial-LIC-R
     primer_bind     3496..3529
                     /label=BamHI-WPRE-F
     primer_bind     3509..3529
                     /label=WPRE-F
     misc_feature    3518..4106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3585..3603)
                     /label=A94-WPRE-seq-R
     primer_bind     4045..4064
                     /label=MCS2080
     primer_bind     complement(4175..4192)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4181..4405
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4250..4267)
                     /label=BGH2-R
     rep_origin      4451..4879
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4538..4557)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4748..4769
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4888..4908)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4893..5222
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5073..5208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5135..5154
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5289..6083
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5343..5362)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5953..5972
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6257..6378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6294..6313)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6348..6367
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6357..6396)
                     /label=Sal1-mut1-R
     primer_bind     6397..6433
                     /label=Sal1-mut1-F
     primer_bind     complement(6427..6443)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6440..6462)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(6658..6675)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6829..7417)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6909..6928)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7588..8448)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8211..8230
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8449..8553)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8554..8583)
                     /label=Sal1-mut2-R
     primer_bind     8584..8613
                     /label=Sal1-mut2-F
     primer_bind     complement(8628..8647)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8655..8674
                     /label=back-seq-F
ORIGIN
        1 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      421 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      481 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      541 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
      601 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
      661 tggctagcgc cgccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc
      721 cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg
      781 tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt
      841 acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc
      901 atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc
      961 tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga
     1021 acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga
     1081 tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga
     1141 ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct
     1201 tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca
     1261 tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt
     1321 gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg
     1381 ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact
     1441 tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca
     1501 gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg
     1561 ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg
     1621 gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca
     1681 tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg
     1741 acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact
     1801 tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca
     1861 tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg
     1921 gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg
     1981 accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga
     2041 gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg
     2101 atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga
     2161 aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg
     2221 ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc
     2281 gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgtaagaa ttcgcttatg
     2341 cggccgcctc ctttaagaag gagatataga tctctgtagg tcccaaacaa gctagtctta
     2401 atggagtcac attaattgga gaagccgtaa aaacacagtt caattattat aagaaagttg
     2461 atggtgttgt ccaacaatta cctgaaactt actttactca gagtagaaat ttacaagaat
     2521 ttaaacccag gagtcaaatg gaaattgatt tcttagaatt agctatggat gaattcattg
     2581 aacggtataa attagaaggc tatgccttcg aacatatcgt ttatggagat tttagtcata
     2641 gtcagttagg tggtttacat ctactgattg gactagctaa acgttttaag gaatcacctt
     2701 ttgaattaga agattttatt cctatggaca gtacagttaa aaactatttc ataacagatg
     2761 cgcaaacagg ttcatctaag tgtgtgtgtt ctgttattga tttattactt gatgattttg
     2821 ttgaaataat aaaatcccaa gatttatctg tagtttctaa ggttgtcaaa gtgactattg
     2881 actatacaga aatttcattt atgctttggt gtaaagatgg ccatgtagaa acattttacc
     2941 caaaattaca atctagtcaa gcgtggcaac cgggtgttgc tatgcctaat ctttacaaaa
     3001 tgcaaagaat gctattagaa aagtgtgacc ttcaaaatta tggtgatagt gcaacattac
     3061 ctaaaggcat aatgatgaat gtcgcaaaat atactcaact gtgtcaatat ttaaacacat
     3121 taacattagc tgtaccctat aatatgagag ttatacattt tggtgctggt tctgataaag
     3181 gagttgcacc aggtacagct gttttaagac agtggttgcc tacgggtacg ctgcttgtcg
     3241 attcagatct taatgacttt gtctctgatg cagattcaac tttgattggt gattgtgcaa
     3301 ctgtacatac agctaataaa tgggatctca ttattagtga tatgtacgac cctaagacta
     3361 aaaatgttac aaaagaaaat gactctaaag agggtttttt cacttacatt tgtgggttta
     3421 tacaacaaaa gctagctctt ggaggttccg tggctataaa gatagatccc aactccataa
     3481 gtgtgtcgac atataaggat ccactcgacg actcgacaat caacctctgg attacaaaat
     3541 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3601 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3661 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3721 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3781 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3841 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3901 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     3961 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4021 cggcctgctg ccggctctgc ggcctcttcc gcatcttcgc cttcgccctc agacgagtcg
     4081 gatctccctt tgggccgcct ccccgcctgg aattaattcg agctcggtac cttgattctc
     4141 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     4201 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4261 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4321 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4381 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     4441 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4501 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4561 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4621 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4681 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4741 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4801 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4861 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4921 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4981 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5041 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5101 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5161 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5221 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     5281 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5341 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5401 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5461 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5521 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5581 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5641 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5701 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5761 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5821 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5881 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5941 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6001 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6061 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     6121 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6181 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6241 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6301 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6361 actcatcaat gtatcttatc atgtctgtat accctccacc tctagctaga gcttggcgta
     6421 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6481 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6541 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6601 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6661 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6721 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6781 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6841 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6901 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6961 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7021 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7081 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7141 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7201 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7261 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7321 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7381 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7441 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7501 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7561 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7621 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7681 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7741 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7801 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7861 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7921 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7981 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8041 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8101 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8161 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8221 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8281 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8341 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8401 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8461 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8521 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8581 cctccacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     8641 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     8701 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     8761 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcg
//
