LOCUS       Exported                9169 bp DNA     circular SYN 04-APR-2021
DEFINITION  Express SARS-CoV-2 spike protein with C9 tag at C-terminal in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3.1-SARS2-Spike
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Shang J, Ye G, Shi K, Wan Y, Luo C, Aihara H, Geng Q, Auerbach A, Li
            F
  TITLE     Structural basis of receptor recognition by SARS-CoV-2.
  JOURNAL   Nature. 2020 Mar 30. pii: 10.1038/s41586-020-2179-y. doi: 
            10.1038/s41586-020-2179-y.
  PUBMED    32225175
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 13, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 Mar 30. pii: 10.1038/s41586-020-2179-y. doi: 
            10.1038/s41586-020-2179-y."
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        79..458
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        459..662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     480..499
                     /label=CMV2-F
     primer_bind     613..633
                     /label=CMV-F
     primer_bind     675..694
                     /label=P675
                     /note="0,55.2362196487738"
     primer_bind     707..726
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        707..725
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             745..4566
                     /codon_start=1
                     /label=Spike
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSA
                     NNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNE
                     NGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGE
                     VFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADS
                     FVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK
                     SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFEL
                     LHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAV
                     RDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRV
                     YSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIA
                     YTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLL
                     LQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKP
                     SKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIA
                     QYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSA
                     IGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEA
                     EVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYH
                     LMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQR
                     NFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDL
                     GDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLI
                     AIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     primer_bind     1444..1464
                     /label=P1443
                     /note="0,58.1409760941563"
     primer_bind     2112..2131
                     /label=P2112
                     /note="0,57.4100439340944"
     primer_bind     2444..2463
                     /label=P2443
                     /note="0,58.4443001137883"
     primer_bind     complement(2560..2577)
                     /label=SDM-D614G-R
     primer_bind     2578..2600
                     /label=SDM-D614G-F
     primer_bind     3210..3229
                     /label=P3209
                     /note="0,56.9256282548245"
     primer_bind     3825..3844
                     /label=P3824
                     /note="0,59.5600493628916"
     primer_bind     4389..4408
                     /label=P4388
                     /note="0,59.2846389371423"
     polyA_signal    4610..4834
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4679..4696)
                     /label=BGH2-R
     rep_origin      4880..5308
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4967..4986)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4968..4989
                     /label=P5000
                     /note="0,58.192814300722"
     primer_bind     5177..5198
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5317..5337)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5322..5651
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5502..5637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5564..5583
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5603..5624
                     /label=P5635
                     /note="0,57.0047839820589"
     CDS             5718..6512
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5772..5791)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6147..6159)
                     /label=P3824
                     /note="0,59.5600493628916"
     primer_bind     6187..6207
                     /label=P6219
                     /note="0,57.5037740458499"
     primer_bind     6382..6401
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6686..6807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6723..6742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6777..6796
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6856..6872)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6856..6872)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6869..6891)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6880..6896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6904..6934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6949..6970
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7087..7104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7258..7846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7338..7357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7409..7427
                     /label=P7441
                     /note="0,57.6683350070133"
     misc_feature    7428..8422
                     /label=Feature 18
     CDS             complement(8017..8877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8591..8611
                     /label=P8623
                     /note="0,58.6933551222971"
     primer_bind     8640..8659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8878..8982)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9057..9076)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9084..9103
                     /label=back-seq-F
     primer_bind     9113..9132
                     /label=P9145
                     /note="0,59.8747639630097"
ORIGIN
        1 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
       61 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      121 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      181 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      241 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      301 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      361 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      421 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      661 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
      721 atagggagac ccaagctggc tagcatgttt gtcttcctgg tcctgctgcc tctggtctcg
      781 tctcagtgcg tgaacctgac tactagaacc cagctgcctc ctgcctatac taactccttc
      841 acccgcggcg tgtactaccc agacaaggtg ttccgcagct ccgtgctgca ctccacccag
      901 gatctgttcc tgcccttctt cagcaacgtg acctggttcc acgccatcca cgtgagcggc
      961 accaatggca ccaagcggtt cgacaatccc gtgctgccat tcaacgatgg cgtgtacttc
     1021 gcctccaccg agaagagcaa catcatccgc ggctggatct tcggcaccac cctggactcc
     1081 aagacccaga gcctgctgat cgtgaacaat gccaccaacg tggtcatcaa ggtgtgcgag
     1141 ttccagttct gcaatgatcc attcctgggc gtgtactacc acaagaacaa taagtcctgg
     1201 atggagagcg agttccgcgt gtacagctcc gccaacaatt gcaccttcga gtacgtgtcc
     1261 cagcccttcc tgatggacct ggagggcaag cagggcaatt tcaagaacct gcgcgagttc
     1321 gtgttcaaga atatcgatgg ctacttcaag atctactcca agcacacccc catcaacctg
     1381 gtgcgcgacc tgccacaggg cttcagcgcc ctggagccac tggtggatct gccaatcggc
     1441 atcaacatca ccaggttcca gaccctgctg gccctgcacc gcagctacct gaccccaggc
     1501 gacagctcca gcggatggac cgctggagct gctgcctact acgtgggcta cctgcagccc
     1561 cgcaccttcc tgctgaagta caacgagaat ggcaccatca ccgacgccgt ggattgcgcc
     1621 ctggatccac tgtccgagac aaagtgcacc ctgaagagct tcaccgtgga gaagggcatc
     1681 taccagacct ccaatttccg cgtgcagcca accgagagca tcgtgcgctt ccccaatatc
     1741 accaacctgt gcccattcgg cgaggtgttc aacgctacca ggttcgccag cgtgtacgct
     1801 tggaatcgca agcgcatctc caactgcgtg gccgactaca gcgtgctgta caactccgcc
     1861 agcttctcca ccttcaagtg ctacggcgtg tcccccacca agctgaatga tctgtgcttc
     1921 accaacgtgt acgccgatag cttcgtgatc aggggcgacg aggtgcgcca gatcgctcca
     1981 ggacagaccg gcaagatcgc tgactacaat tacaagctgc ccgacgattt caccggctgc
     2041 gtgatcgcct ggaactccaa caatctggat agcaaagtgg gcggcaacta caattacctg
     2101 taccgcctgt tccgcaagtc caatctgaag ccattcgagc gcgacatctc caccgagatc
     2161 taccaggctg gaagcacccc atgcaatgga gtggagggct tcaactgcta cttccccctg
     2221 cagagctacg gcttccagcc aaccaacgga gtgggatacc agccatacag ggtggtggtg
     2281 ctgtccttcg agctgctgca cgctccagct accgtgtgcg gaccaaagaa gagcaccaat
     2341 ctggtgaaga acaagtgcgt gaacttcaat ttcaacggcc tgaccggaac cggcgtgctg
     2401 accgagtcca acaagaagtt cctgccattc cagcagttcg gaagggacat cgctgatacc
     2461 accgacgccg tgcgcgaccc acagaccctg gagatcctgg atatcacccc atgctccttc
     2521 ggcggcgtga gcgtgatcac cccaggaacc aataccagca accaggtggc cgtgctgtac
     2581 cagggcgtga attgcaccga ggtgccagtg gctatccacg ctgatcagct gaccccaacc
     2641 tggcgcgtgt acagcaccgg atccaacgtg ttccagaccc gcgccggatg cctgatcgga
     2701 gctgagcacg tgaacaattc ctacgagtgc gacatcccaa tcggagctgg aatctgcgcc
     2761 agctaccaga cccagaccaa ctccccaagg agggctcgca gcgtggccag ccagtccatc
     2821 atcgcctaca ccatgtccct gggcgccgag aatagcgtgg cctacagcaa caattccatc
     2881 gccatcccaa ccaacttcac catctccgtg accaccgaga tcctgcccgt gtccatgacc
     2941 aagaccagcg tggactgcac catgtacatc tgcggcgatt ccaccgagtg cagcaacctg
     3001 ctgctgcagt acggcagctt ctgcacccag ctgaatcgcg ccctgaccgg aatcgctgtg
     3061 gagcaggata agaacaccca ggaggtgttc gcccaggtga agcagatcta caagaccccc
     3121 ccaatcaagg acttcggcgg cttcaatttc agccagatcc tgcccgatcc aagcaagccc
     3181 tccaagcgca gcttcatcga ggacctgctg ttcaacaagg tgaccctggc cgatgccggc
     3241 ttcatcaagc agtacggcga ttgcctgggc gacatcgctg cccgcgacct gatctgcgcc
     3301 cagaagttca atggcctgac cgtgctgcca ccactgctga ccgatgagat gatcgctcag
     3361 tacacctccg ccctgctggc cggaaccatc accagcggat ggaccttcgg cgctggagcc
     3421 gccctgcaga tccccttcgc catgcagatg gcctaccgct tcaacggcat cggcgtgacc
     3481 cagaatgtgc tgtacgagaa ccagaagctg atcgccaatc agttcaactc cgccatcggc
     3541 aagatccagg actccctgtc cagcaccgcc agcgccctgg gcaagctgca ggatgtggtg
     3601 aatcagaacg cccaggccct gaataccctg gtgaagcagc tgtccagcaa cttcggcgcc
     3661 atctccagcg tgctgaatga tatcctgagc cgcctggaca aggtggaggc tgaggtgcag
     3721 atcgataggc tgatcaccgg ccgcctgcag tccctgcaga cctacgtgac ccagcagctg
     3781 atcagggctg ctgagatcag ggccagcgcc aatctggctg ctaccaagat gtccgagtgc
     3841 gtgctgggac agagcaagag ggtggacttc tgcggcaagg gctaccacct gatgtccttc
     3901 ccacagagcg ccccacacgg agtggtgttc ctgcacgtga cctacgtgcc agcccaggag
     3961 aagaacttca ccaccgctcc agctatctgc cacgatggca aggctcactt cccacgcgag
     4021 ggcgtgttcg tgtccaacgg cacccactgg ttcgtgaccc agcgcaattt ctacgagccc
     4081 cagatcatca ccaccgacaa taccttcgtg agcggcaact gcgacgtggt catcggaatc
     4141 gtgaacaata ccgtgtacga tcccctgcag ccagagctgg actccttcaa ggaggagctg
     4201 gataagtact tcaagaatca caccagcccc gacgtggatc tgggcgacat ctccggcatc
     4261 aatgccagcg tggtgaacat ccagaaggag atcgaccgcc tgaacgaggt ggccaagaat
     4321 ctgaacgagt ccctgatcga tctgcaggag ctgggcaagt acgagcagta catcaagtgg
     4381 ccatggtaca tctggctggg cttcatcgcc ggcctgatcg ccatcgtgat ggtgaccatc
     4441 atgctgtgct gcatgacctc ctgctgcagc tgcctgaagg gctgctgctc ctgcggcagc
     4501 tgctgcaagt tcgatgagga cgatagcgag cccgtgctga agggcgtcaa actgcactat
     4561 acatagctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     4621 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4681 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4741 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4801 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     4861 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4921 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4981 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5041 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5101 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5161 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5221 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5281 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     5341 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5401 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5461 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5521 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5581 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5641 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     5701 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     5761 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5821 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5881 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     5941 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6001 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6061 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6121 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6181 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6241 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6301 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6361 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6421 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6481 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     6541 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     6601 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6661 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     6721 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6781 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     6841 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6901 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6961 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7021 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7081 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7141 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7201 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7261 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7321 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7381 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7441 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7501 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7561 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7621 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7681 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7741 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7801 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7861 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7921 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7981 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8041 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8101 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8161 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8221 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8281 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8341 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8401 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8461 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8521 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8581 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8641 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8701 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8761 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8821 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8881 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8941 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9001 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     9061 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     9121 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttga
//
