LOCUS       proUSER13F4C            9961 bp ds-DNA     circular     23-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      28..132
                     /label="Terminator 1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    142..2432
                     /label="manR & PmanA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             345..2291
                     /label="manR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2441..4232
                     /label="ProUSER2.0 Cassette"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      4279..4381
                     /label="Terminator 0"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(4516..5286)
                     /label="KanR-BS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5524..6410
                     /label="sigF-DS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             6579..7439
                     /label="bla (AmpR)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             7494..7739
                     /label="oriT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(7748..8410)
                     /label="BBR1 rep"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    9292..9961
                     /label="sigF-US"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 accagcttgt acaggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctttaatta aagcggataa caatttcaca caggaggccg atcgcgtcat
      181 agcaaactca aagagtataa aaatcgcttt tttccggaag cttcggtaaa aaacgaaact
      241 tttgtctcta tgattttgtt ttataatgta aacggtttct tatatagtat acttatacta
      301 tcaatttgct caagtagata ctgacaggaa ggatagaaaa acagatggaa tacataaata
      361 caagacaaaa agagattttg tatctgttgt tatctgaacc tgatgactat ttagttgtgc
      421 aagatttcgc agatagggta cagtgttctg aaaaaacaat ccggaatgat ttgaaagtga
      481 tcgaggatta tctcaatgag cattctcatg cacagcttat tcgaaaacca gggttgggcg
      541 tttatttaca tatagaagaa caggaaagga cctggctaag ccaacaatta catactgaac
      601 atttttcttc taggcagcgg agcgataagg aaagaatgct gcacattgct tatgacttat
      661 taatgaatcc gaagcctgtt tccgcaaaag acattgccgc tcggcatttt gtcaatcgat
      721 cttccattaa aaaggattta tacgcagtag aagagtggct gaagcgtttt gatctcacac
      781 tggtttctag gcagcggctt ggattaaagg ttgaagggaa cgaaagaaat aaaagaaagg
      841 cattggcaag aatatctgat ttgattcaca atacagcgtt taccagccaa tttattaaaa
      901 gtaagttttt acattatgaa gtagactttg tgacgaaaga aatcaaatca ctacaaaaaa
      961 agcattccct ctatttcaca gacgaaacgt ttgaaagttt gttgctgcat acattgttaa
     1021 tggtccgccg catcaaaatg aagcagccga tttcattgtc cccaaaagaa atggcggcag
     1081 taaaaaagaa gaaagaatat caatggacat ttgcttgttt acagcggctt gaaccggttt
     1141 ttgcaattcg cttccctgaa gaagaagccg tgtacttaac attacatata ttgggcggta
     1201 aggttcgtta tccattgcag acagaagaga accttgaaaa tgctgtgctt cctaaggttg
     1261 tagggcattt aatcaatcgt gtttcagagc tgaaaatgat ggattttcac aaggatcagg
     1321 atttgatcaa tggtttgaac attcatctca atactgtgct acaacggctg agctacgatc
     1381 tttctgtagc aaatcctatg ctcaatgaca tcaaaaagat gtacccttat ttgttccacc
     1441 tcatcattga tgttttggaa gacatcaatc aaacttttga tcttcatatt cctgaggaag
     1501 aggcagcata tttgacattg cattttcagg cagcgatcga acggatgcag ggcagcagcg
     1561 agacacataa aaaggcggtt atcgtctgcc acatggggat tgggatgtca caattgctgc
     1621 ggacaaaaat tgaacggaaa tatcaccaaa tcgctgtaat ggcttgtatt gcaaaggccg
     1681 atttaaagga ttatatcaaa aagcatgagg atattgatct tgtcatatcc accattgcgc
     1741 tggagaatat aacggttccc catattgttg tgtctccgct tttagagcct ggcgaagaga
     1801 agaaattaag tgcgtttata cgtcagctgg gcgaatcaca ccgccaaaaa caaaaaacgt
     1861 ttcagatgct gaacaatacg actccttttt tggttttttt gcagcaggag gcggagcatc
     1921 gctacaagtt aatcgaacag ctggcgacag ctttgtttga gaaaggctat gttgataagg
     1981 actatgcggt tcatgcggtg atgagggaaa aaatgtcggc tacaaatatt gggtcaggca
     2041 ttgccattcc tcatgcaaac gctaaattta ttaaacaatc agcaatcgcg attgccacat
     2101 taaaggagcc gcttgaatgg ggaaataaaa aagtttcgct cgtttttatg ctggctgtca
     2161 aacacgagga tcaaactatg acaaagcagc tgtttagcga gctttcatat cttagcgagc
     2221 agccggcctt cgtccagaag ctgacgaaag aaaccaatgt catgacattt ttatctcatt
     2281 tggattatta aaagcaggga ttattccttg ctttttttgt tatagggaaa aatgccttta
     2341 ttaccggaac ctatggtaaa aaaagcgatt ttaatgagct gatttcggta tacagttgag
     2401 acaagatctt attattcaca ctttaagctg aggcgatcgc aacgtcgtga ctgggaaaac
     2461 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     2521 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     2581 cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt
     2641 cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc
     2701 atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat
     2761 ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag
     2821 acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg
     2881 gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc
     2941 gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa
     3001 gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg
     3061 actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct
     3121 gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt
     3181 tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt
     3241 atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg
     3301 aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc
     3361 gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa
     3421 aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag
     3481 catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg
     3541 atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg
     3601 tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac
     3661 ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa
     3721 cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg
     3781 gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc
     3841 gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt
     3901 atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg
     3961 tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac
     4021 gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag
     4081 tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat
     4141 gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat
     4201 cgccagttct gtatgaacgg tctggtcttt gcgcgatcgc acctcagcgt cgtgactggg
     4261 aaaaccctgg cgactagtct tggactcctg ttgatagatc cagtaatgac ctcagaactc
     4321 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
     4381 gacgtgtagg ctggagctgc ttccctgcag gagtcaggtt ttatattgcg aaaaataatt
     4441 ggcgaacgag gtaactggat acctcatccg ccaattaaaa tttgctgtgc aacaggtgac
     4501 atcagaggtc atcgttcaaa atggtatgcg ttttgacaca tccactatat atccgtgtcg
     4561 ttctgtccac tcctgaatcc cattccagaa attctctagc gattccagaa gtttctcaga
     4621 gtcggaaagt tgaccagaca ttacgaactg gcacagatgg tcataacctg aaggaagatc
     4681 tgattgctta actgcttcag ttaagaccga agcgctcgtc gtataacaga tgcgatgatg
     4741 cagaccaatc aacatggcac ctgccattgc tacctgcaca gtcaaggatg gtagaaatgt
     4801 tgtcggtcct tgcacacgaa tattacgcca tttgcctgca tattcaaaca gctcttctac
     4861 gataagggca caaatcgcat cgtggaacgt ttgggcttct accgatttag cagtttgata
     4921 cactttctct aagtatccac ctgaatcata aatcggcaaa atagagaaaa attgaccatg
     4981 tgtaagcggc caatctgatt ccacctgaga tgcataatct agtagaatct cttcgctatc
     5041 aaaattcact tccaccttcc actcaccggt tgtccattca tggctgaact ctgcttcctc
     5101 tgttgacatg acacacatca tctcaatatc cgaatagggc ccatcagtct gacgaccaag
     5161 agagccataa acaccaatag ccttaacatc atccccatat ttatccaata ttcgttcctt
     5221 aatttcatga acaatcttca ttctttcttc tctagtcatt attattggtc cattcactat
     5281 tctcattccc ctttcagata attttagatt tgcttttcta aataagaata tttggagagc
     5341 accgttctta ttcagctatt aaacccatta tatcgggttt ttgaggggat ttcaactgca
     5401 gcgttgtcct tttccgctgt ggttgttttt gttggaagtg tgtatcaggt atctgcatag
     5461 ttattccgaa cttccaatta atgcaagctg gtaccctaag gaggatattc atatgcaagc
     5521 acttctgcag tgcaggctag cttttttgtg caaaagcgtg gtaatttatg gtcttttcga
     5581 gcggatgaat gagaacaaaa tcgaaccaca tactacatat ataaccaccg aaagatggtg
     5641 atcaatgatg gaacgacgaa tatttatccg gcttcgccac cgagtgctgg cacatccagg
     5701 ggatattatt accgttggag atgccgcgca aatagaaggg cagcttcagc tgaaaaagaa
     5761 actttcggct atgccgcttt atcaggtgag cgaaaaagat aaaaatatcg taattctgga
     5821 tatcatacaa gtcctcagag ccattcattt acaagacccg acaattgatg ttcaaaccgt
     5881 aggcggagca gaaaccattg ttgaaattca gtatcgaaag cgaaatttat caacggttct
     5941 atttatcggt gtctggctgc ttctgtttat tggatcgtgt cttgccatca tgaactttca
     6001 tgaggatgta agcatgagag atgttcatat cgcactatat gaaatcataa ccggagagag
     6061 gaatgactat ccatatttgc ttcaaatccc atacagcatc ggtttgggac tggggatgat
     6121 cgtgtttttt aaccacatat ttaaaaagcg cctaaatgaa gagcccagcc cgctggaggt
     6181 tgagatgttt aactatcagc ttgatctcga tcaatatgtg gccatgcatg agaatcaaga
     6241 aaccataaag gatctgcatg atcgttagtg tattgttcat catttttgtc gggctcggcg
     6301 gaggcataac ggtgggggcg ggtttcgttg cttttttaac cgtaatggga atcattccgc
     6361 ggctgatgca gctcaccaaa acaatgagat ttgttcaggc ttatgaagcg acctgactgg
     6421 gtccccaata attacgattt aaattagtag cccgcctaat gagcgggctt ttttttaatt
     6481 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6541 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagcattcag cattttcgtg
     6601 tggcgctgat tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat ccggaaaccc
     6661 tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat attgaactgg
     6721 atctgaacag cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt ccgatgatga
     6781 gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg ggccaggaac
     6841 aactgggccg tcgtattcat tatagccaga acgatctggt ggaatatagc ccggtgaccg
     6901 aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg attaccatga
     6961 gcgataacac cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa gaactgaccg
     7021 cgtttctgca taacatgggc gatcatgtga cccgtctgga tcgttgggaa ccggaactga
     7081 acgaagcgat tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg gcgaccaccc
     7141 tgcgtaaact gctgacgggt gagctgctga ccctggcaag ccgccagcaa ctgattgatt
     7201 ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg gctggctggt
     7261 ttattgcgga taaaagcggt gcgggcgaac gtggcagccg tggcattatt gcggcgctgg
     7321 gcccggatgg taaaccgagc cgtattgtgg tgatttatac caccggcagc caggcgacga
     7381 tggatgaacg taaccgtcag attgcggaaa ttggcgcgag cctgattaaa cattggtaaa
     7441 ccgatacaat taaaggctcc ttttggagcc tttttttttg gacgaccctt gtccttttcc
     7501 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc
     7561 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg
     7621 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact
     7681 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag
     7741 gccggcccta ccggcgcggc agcgttaccc gtgtcggcgg ctccaacggc tcgccatcgt
     7801 ccagaaaaca cggctcatcg ggcatcggca ggcgctgctg cccgcgccgt tcccattcct
     7861 ccgtttcggt caaggctggc aggtctggtt ccatgcccgg aatgccgggc tggctgggcg
     7921 gctcctcgcc ggggccggtc ggtagttgct gctcgcccgg atacagggtc gggatgcggc
     7981 gcaggtcgcc atgccccaac agcgattcgt cctggtcgtc gtgatcaacc accacggcgg
     8041 cactgaacac cgacaggcgc aactggtcgc ggggctggcc ccacgccacg cggtcattga
     8101 ccacgtaggc cgacacggtg ccggggccgt tgagcttcac gacggagatc cagcgctcgg
     8161 ccaccaagtc cttgactgcg tattggaccg tccgcaaaga acgtccgatg agcttggaaa
     8221 gtgtcttctg gctgaccacc acggcgttct ggtggcccat ctgcgccacg aggtgatgca
     8281 gcagcattgc cgccgtgggt ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc
     8341 gttccgtttg cacccagtga ccgggcttgt tcttggcttg aatgccgatt tctctggact
     8401 gcgtggccat gcttatctcc atgcggtagg ggtgccgcac ggttgcggca ccatgcgcaa
     8461 tcagctgcaa cttttcggca gcgcgacaac aattatgcgt tgcgtaaaag tggcagtcaa
     8521 ttacagattt tctttaacct acgcaatgag ctattgcggg gggtgccgca atgagctgtt
     8581 gcgtaccccc cttttttaag ttgttgattt ttaagtcttt cgcatttcgc cctatatcta
     8641 gttctttggt gcccaaagaa gggcacccct gcggggttcc cccacgcctt cggcgcggct
     8701 ccccctccgg caaaaagtgg cccctccggg gcttgttgat cgactgcgcg gccttcggcc
     8761 ttgcccaagg tggcgctgcc cccttggaac ccccgcactc gccgccgtga ggctcggggg
     8821 gcaggcgggc gggcttcgcc cttcgactgc ccccactcgc ataggcttgg gtcgttccag
     8881 gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc cttgacccgc cttccacttg
     8941 gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa
     9001 ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt
     9061 cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg
     9121 tccatcagct tgtccagcag ggttgtccac gggccgagcg aagcgagcca gccggtggcc
     9181 gctcgcggcc atcgtccaca tatccacggg ctggcaaggg agcgcagcga ccgcgcaggg
     9241 cgaagcccgg agagcaagcc cgtaggggga ggcgatctcg agagcttgca tgctgaactt
     9301 ggaggacctt tcctttatgg acagctcggg gcttggcgtt attttaggaa gatacaagca
     9361 aattaagcaa attggcggag aaatggttgt ttgcgctatc tctcctgcgg tgaagcgatt
     9421 gtttgatatg tcgggtctgt ttaaaattat ccgatttgaa caatctgaac agcaggcact
     9481 cctgacactg ggggtggcat catgaaaaat gaaatgcacc ttgagttttc tgccctcagt
     9541 cagaatgaat cgttcgcccg tgtgacagtt gcttcattta tagctcagct ggacccgaca
     9601 atggatgaac tgactgaaat caaaacagtc gtgtcagagg ctgtcacgaa tgcgattatc
     9661 catggatatg aagagaactg tgaagggaaa gtttacattt cagtgacgct ggaagatcat
     9721 gtcgtatata tgactattcg tgatgaaggc ttaggcatta cagatcttga agaagcccgt
     9781 cagcctctat ttacgactaa gcctgagctt gagcgctctg gaatgggctt taccattatg
     9841 gaaaatttca tggatgatgt cagtatcgat tcatcgcctg aaatgggaac aacgattcgc
     9901 ttaacaaagc acttatcaaa aagcaaagcg ctttgtaatt aaggagattt gttatggatt
     9961 a
//