LOCUS       proUSER13E3F            8859 bp ds-DNA     circular     23-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      28..132
                     /label="Terminator 1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(178..804)
                     /label="tetR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    178..988
                     /label="Ptet & TetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    997..2788
                     /label="ProUSER2.0 Casette"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2835..2937
                     /label="Terminator 0"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3331..4113
                     /label="SpcR-BS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4383..5162
                     /label="yhgE-DS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             5331..6191
                     /label="bla (AmpR)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             6246..6491
                     /label="oriT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(6500..7162)
                     /label="BBR1 rep"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    8044..8859
                     /label="yhgE US"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 accagcttgt acaggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctttaatta aagcggataa caatttcaca caggaggccg atcgcgttta
      181 agacccactt tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa
      241 taagaaggct ggctctgcac cttggtgatc aaataattcg atagcttgtc gtaataatgg
      301 cggcatacta tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgatc
      361 ttccaatacg caacctaaag taaaatgccc cacagcgctg agtgcatata atgcattctc
      421 tagtgaaaaa ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt
      481 ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca
      541 tctaaaactt ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca
      601 aaagtgagta tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt
      661 attttttaca tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg
      721 ggttgttaaa ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt
      781 acttttatct aatctagaca tcattaattc ctcctttttg ttgacattat atcattgata
      841 gagttatttg tcaaactaga tttttatttc gatgccctgg acttcatgaa aaactaaaaa
      901 aaatattgac actctatcat tgatagagta taattaaaat aagcttgatc gtagcgttaa
      961 cagatctgag ctcctgcagt aagctgaggc gatcgcaacg tcgtgactgg gaaaaccctg
     1021 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     1081 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
     1141 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
     1201 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
     1261 acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
     1321 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc
     1381 gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg
     1441 gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg
     1501 gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc
     1561 aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta
     1621 cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac
     1681 tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt
     1741 tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg
     1801 atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac
     1861 tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg
     1921 gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg
     1981 gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc
     2041 atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga
     2101 agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca
     2161 cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca
     2221 tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg
     2281 taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga
     2341 atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc
     2401 cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt
     2461 gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca
     2521 tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc
     2581 acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc
     2641 cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg
     2701 aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc
     2761 agttctgtat gaacggtctg gtctttgcgc gatcgcacct cagcgtcgtg actgggaaaa
     2821 ccctggcgac tagtcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     2881 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccagacg
     2941 tgtaggctgg agctgcttcc ctgcaggagt caggtcaatc atcagataac tatggcggca
     3001 cgtgcattaa ccacggttgt atcccgtcta aagtactcgt ctgtgcaaca ggtgacatca
     3061 cgaaacgatc ctcatcctgt ctcttgatcc atggattacg cgttaacccg ggcccgcgga
     3121 tgcatatgat cagatcttaa ggcctaggtc tagaggatcg atctgtataa taaagaataa
     3181 ttattaatct gtagacaaat tgtgaaagga tgtacttaaa cgctaacggt cagctttatt
     3241 gaacagtaat ttaagtatat gtccaatcta gggtaagtaa attgagtatc aatataaact
     3301 ttatatgaac ataatcaacg aggtgaaatc atgagcaatt tgattaacgg aaaaatacca
     3361 aatcaagcga ttcaaacatt aaaaatcgta aaagatttat ttggaagttc aatagttgga
     3421 gtatatctat ttggttcagc agtaaatggt ggtttacgca ttaacagcga tgtagatgtt
     3481 ctagtcgtcg tgaatcatag tttacctcaa ttaactcgaa aaaaactaac agaaagacta
     3541 atgactatat caggaaagat tggaaatacg gattctgtta gaccacttga agttacggtt
     3601 ataaatagga gtgaagttgt cccttggcaa tatcctccaa aaagagaatt tatatacggt
     3661 gagtggctca ggggtgaatt tgagaatgga caaattcagg aaccaagcta tgatcctgat
     3721 ttggctattg ttttagcaca agcaagaaag aatagtattt ctctatttgg tcctgattct
     3781 tcaagtatac ttgtctccgt acctttgaca gatattcgaa gagcaattaa ggattctttg
     3841 ccagaactaa ttgaggggat aaaaggtgat gagcgtaatg taattttaac cctagctcga
     3901 atgtggcaaa cagtgactac tggtgaaatt acctcgaaag atgtcgctgc agaatgggct
     3961 atacctcttt tacctaaaga gcatgtaact ttactggata tagctagaaa aggctatcgg
     4021 ggagagtgtg atgataagtg ggaaggacta tattcaaagg tgaaagcact cgttaagtat
     4081 atgaaaaatt ctatagaaac ttctctcaat taggctaatt ttattgcaat aacaggtgct
     4141 tacttttctg gagttcttta gcaaattttt ttattagctg aacttagtat tagtggccat
     4201 actcctccaa tccaaagcta tttagaaaga ttactatatc ctcaaacagg cggtaacgcg
     4261 ttgtcctttt ccgctttaat aaactatgga agtatgtaca gtcttgcaat gttgagtgaa
     4321 caaacttcca taataaaata tgcaagctgg taccctaagg aggatattca tatgcaagca
     4381 ctagaaaaag aacttgtttc cttggaaata agttcttttt ttgttacatc ccccatttaa
     4441 taagcaatcc tgtataggtt aatccgccgc caaacccgaa aagcaaaacg atttgatctt
     4501 ttttcagctt cccggctttc actgcgagat cgagcgccaa aacaattgaa accgaagacg
     4561 tgtttccgta gtgctcaaca ctagtgagcg ttttttcaat cgggaacggt gttttttcac
     4621 aaattgactc gatcatgcgc aagttggcgc tgtgaggaac aaaccaatcg agatcatcgg
     4681 agctgagtcc tgctttatgt aaaagccgtt caaattcgcc agggacggtt cttgcggccc
     4741 atttatatac ctcgcgtccg ttttgcacca tttttccgga accgacaagc tgcacaccgt
     4801 ttatttcatt tcgcagtccg gcacgataca aaatatcgcc gccgttcccg cttgttcctt
     4861 gtacagacgc aagaaatccc ggcgtctctt catctcgttc tactaacagc gcacccgcgg
     4921 catcgccgaa cagtacgcat gtcgttcgat cggtataatc ggttaccttt gataacgtct
     4981 ctccggcgat gacgagaatt ttttgatgaa ggccagatgt gatcaatcca tttgccaaat
     5041 ggaggccgta tgtcagcccg gcgcatgtcg cattaatatc cagcgcgccg gtgctttccc
     5101 agccgaaata ttcctgtacg cggcatgccg tactcggaaa ggcgtaatcg gatgtggttg
     5161 tgacctgact gggtccccaa taattacgat ttaaattagt agcccgccta atgagcgggc
     5221 ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5281 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagcattc
     5341 agcattttcg tgtggcgctg attccgtttt ttgcggcgtt ttgcctgccg gtgtttgcgc
     5401 atccggaaac cctggtgaaa gtgaaagatg cggaagatca actgggtgcg cgcgtgggct
     5461 atattgaact ggatctgaac agcggcaaaa ttctggaatc ttttcgtccg gaagaacgtt
     5521 ttccgatgat gagcaccttt aaagtgctgc tgtgcggtgc ggttctgagc cgtgtggatg
     5581 cgggccagga acaactgggc cgtcgtattc attatagcca gaacgatctg gtggaatata
     5641 gcccggtgac cgaaaaacat ctgaccgatg gcatgaccgt gcgtgaactg tgcagcgcgg
     5701 cgattaccat gagcgataac accgcagcga acctgctgct gacgaccatt ggcggtccga
     5761 aagaactgac cgcgtttctg cataacatgg gcgatcatgt gacccgtctg gatcgttggg
     5821 aaccggaact gaacgaagcg attccgaacg atgaacgtga taccaccatg ccggcagcaa
     5881 tggcgaccac cctgcgtaaa ctgctgacgg gtgagctgct gaccctggca agccgccagc
     5941 aactgattga ttggatggaa gcggataaag tggcgggtcc gctgctgcgt agcgcgctgc
     6001 cggctggctg gtttattgcg gataaaagcg gtgcgggcga acgtggcagc cgtggcatta
     6061 ttgcggcgct gggcccggat ggtaaaccga gccgtattgt ggtgatttat accaccggca
     6121 gccaggcgac gatggatgaa cgtaaccgtc agattgcgga aattggcgcg agcctgatta
     6181 aacattggta aaccgataca attaaaggct ccttttggag cctttttttt tggacgaccc
     6241 ttgtcctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat
     6301 ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg
     6361 tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt
     6421 tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga
     6481 ggctggccgt aggccggccc taccggcgcg gcagcgttac ccgtgtcggc ggctccaacg
     6541 gctcgccatc gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc
     6601 gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg
     6661 gctggctggg cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg
     6721 tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa
     6781 ccaccacggc ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca
     6841 cgcggtcatt gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga
     6901 tccagcgctc ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga
     6961 tgagcttgga aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca
     7021 cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct
     7081 catgcgcttt gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga
     7141 tttctctgga ctgcgtggcc atgcttatct ccatgcggta ggggtgccgc acggttgcgg
     7201 caccatgcgc aatcagctgc aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa
     7261 agtggcagtc aattacagat tttctttaac ctacgcaatg agctattgcg gggggtgccg
     7321 caatgagctg ttgcgtaccc ccctttttta agttgttgat ttttaagtct ttcgcatttc
     7381 gccctatatc tagttctttg gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc
     7441 ttcggcgcgg ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg
     7501 cggccttcgg ccttgcccaa ggtggcgctg cccccttgga acccccgcac tcgccgccgt
     7561 gaggctcggg gggcaggcgg gcgggcttcg cccttcgact gcccccactc gcataggctt
     7621 gggtcgttcc aggcgcgtca aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc
     7681 gccttccact tggtgtccaa ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt
     7741 ccccagagaa aattaaaaaa attgatgggg caaggccgca ggccgcgcag ttggagccgg
     7801 tgggtatgtg gtcgaaggct gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc
     7861 aggcgcagcc tgtccatcag cttgtccagc agggttgtcc acgggccgag cgaagcgagc
     7921 cagccggtgg ccgctcgcgg ccatcgtcca catatccacg ggctggcaag ggagcgcagc
     7981 gaccgcgcag ggcgaagccc ggagagcaag cccgtagggg gaggcgatct cgagagcttg
     8041 catgacgggt ctaaacaggt ccaaagcgga tcatcaaaac ttgcagacgg atctgcatca
     8101 cttgatacag gtttaggcaa gctattggac ggcacaggag agctgtctag taagctgaag
     8161 gatgcggctg accaaacagg agatatcgat gctgatgatc aaacatacgg catgtttgcg
     8221 gatccggtta aaaccaaaga tgatgccatt cattccgttc ctaactatgg aacagggctg
     8281 acaccttata tcctgtcaat gggcttgtat gtcggcggca tcatgctgac ggttgtcttc
     8341 ccgctgaagg aagcgtcagg acgtccgaga aacggttttg agtggttctt cagcaaattc
     8401 aatgtcatga tgcttgtagg aatcattcaa tcattgattg tggcgacggt tcttctattg
     8461 ggaattggtc ttgaggtaga gagcacgtgg agattttacg tgtttacgat cattacgagt
     8521 ctcgctttcc tggcgataat tcaatttttg gctacaacga tgggcaaccc ggggcgattt
     8581 atcgcagtca ttattttagt gctgcagctg ggagcgagcg gaggaacctt cccgctggaa
     8641 ttgcttccga atttctatca ggtcatccac ggcgcattgc cgatgaccta cagcattaac
     8701 ggattcagag cagtcatttc aaacggtgac tttggctaca tgtggcagat ggccggcgtc
     8761 ttaatcggca ttgccctcgt catgattgca ctaagcatta cttactttac aatgttaagc
     8821 cggaaggaag aaacatcaga agaacagccg gcttcataa
//