LOCUS       proUSER13G2F            8670 bp ds-DNA     circular     23-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      28..132
                     /label="Terminator 1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(178..804)
                     /label="tetR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    178..988
                     /label="Ptet & TetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    997..2788
                     /label="ProUSER Casette"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2835..2937
                     /label="Terminator 0"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3175..3912
                     /label="EryR-BS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4345..5042
                     /label="rsbP-DS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             5211..6071
                     /label="bla (AmpR)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             6126..6371
                     /label="oriT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(6380..7042)
                     /label="BBR1 rep "
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    7924..8670
                     /label="rsbP US"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 accagcttgt acaggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctttaatta aagcggataa caatttcaca caggaggccg atcgcgttta
      181 agacccactt tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa
      241 taagaaggct ggctctgcac cttggtgatc aaataattcg atagcttgtc gtaataatgg
      301 cggcatacta tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgatc
      361 ttccaatacg caacctaaag taaaatgccc cacagcgctg agtgcatata atgcattctc
      421 tagtgaaaaa ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt
      481 ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca
      541 tctaaaactt ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca
      601 aaagtgagta tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt
      661 attttttaca tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg
      721 ggttgttaaa ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt
      781 acttttatct aatctagaca tcattaattc ctcctttttg ttgacattat atcattgata
      841 gagttatttg tcaaactaga tttttatttc gatgccctgg acttcatgaa aaactaaaaa
      901 aaatattgac actctatcat tgatagagta taattaaaat aagcttgatc gtagcgttaa
      961 cagatctgag ctcctgcagt aagctgaggc gatcgcaacg tcgtgactgg gaaaaccctg
     1021 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     1081 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
     1141 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
     1201 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
     1261 acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
     1321 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc
     1381 gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg
     1441 gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg
     1501 gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc
     1561 aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta
     1621 cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac
     1681 tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt
     1741 tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg
     1801 atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac
     1861 tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg
     1921 gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg
     1981 gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc
     2041 atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga
     2101 agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca
     2161 cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca
     2221 tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg
     2281 taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga
     2341 atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc
     2401 cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt
     2461 gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca
     2521 tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc
     2581 acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc
     2641 cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg
     2701 aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc
     2761 agttctgtat gaacggtctg gtctttgcgc gatcgcacct cagcgtcgtg actgggaaaa
     2821 ccctggcgac tagtcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     2881 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccagacg
     2941 tgtaggctgg agctgcttcc ctgcaggagt caggtagacg agaaacgttc cgtccgtctg
     3001 ggtcagttgg gcaaagttga tgaccgggtc gtccgttctg tgcaacaggt gacatcacta
     3061 gagtctaggg acctctttag ctccttggaa gctgtcagta gtatacctaa taatttatct
     3121 acattccctt tagtaacgtg taactttcca aatttacaaa agcgactcat agaattattt
     3181 cctcccgtta aataatagat aactattaaa aatagacaat acttgctcat aagtaacggt
     3241 acttaaattg tttactttgg cgtgtttcat tgcttgatga aactgatttt tagtaaacag
     3301 ttgacgatat tctcgattga cccattttga aacaaagtac gtatatagct tccaatattt
     3361 atctggaaca tctgtggtat ggcgggtaag ttttattaag acactgttta cttttggttt
     3421 aggatgaaag cattccgctg gcagcttaag caattgctga atcgagactt gagtgtgcaa
     3481 gagcaaccct agtgttcggt gaatatccaa ggtacgcttg tagaatcctt cttcaacaat
     3541 cagatagatg tcagacgcat ggctttcaaa aaccactttt ttaataattt gtgtgcttaa
     3601 atggtaagga atactcccaa caattttata cctctgtttg ttagggaatt gaaactgtag
     3661 aatatcttgg tgaattaaag tgacacgagt attcagtttt aatttttctg acgataagtt
     3721 gaatagatga ctgtctaatt caatagacgt tacctgttta cttattttag ccagtttcgt
     3781 cgttaaatgc cctttacctg ttccaatttc gtaaacggta tcggtttctt ttaaattcaa
     3841 ttgttttatt atttggttga gtactttttc actcgttaaa aagttttgag aatattttat
     3901 atttttgttc atgtaatcac tccttcttaa ttacaaattt ttagcatcta atttaacttc
     3961 aattcctatt atacaaaatt ttaagatact gcactatcaa cacactctta agtttgcttc
     4021 taagtcttat ttccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta
     4081 tgatatggtg caagtcagca cgaacacgaa ccgtcttatc tcccattata tctttttttg
     4141 cactgattgg tgtatcattt cgtttttctt tttgtgctag aggatcaatt cttgaagacg
     4201 aaagggcctc gtgatacgcg ttgtcctttt ccgctgtgtt ataaacctgt gtgagagtta
     4261 agtttacatg cctaacctta acttttacgc aggttcagct tatgcaagct ggtaccctaa
     4321 ggaggatatt catatgcaag cacttcagcg tttctttgga atatgtcctg actccgcata
     4381 ttgaaacacc tgcaaggaag gcagcgggtg tccattaaaa tcaaataaag cttgattatc
     4441 tacagcactt cccccatacc acttcccggc gtcttcaggg tcgtattcag cagcatagct
     4501 ggacgcccac cctgacccgt atgtttccca taacactttg tttttctcta tctgtgtctt
     4561 cggcccgact ggaatccacg ccggctccca gtagaaaaca ccaagtcctg ctttgcccgt
     4621 attcgccact gcctccatta catctcttac tgcagttgcc tggccttgaa cagaaattgg
     4681 atatggcaac gtctgcccgc tttttggtgc tgtatttcca tgcccatcgc catcctcagc
     4741 ggtataggtg tacgatgtct ccgccaccat gacttttttg ccgtacgtat tggcaacagc
     4801 cttcagcacg gaggtcaaat tttgtaatgt gccatgccag aaaggataat aggagctagc
     4861 aaacacatca taatccactt tgtttttgct gagtgtttct gcaataaatg aataccttcc
     4921 agccgtttca ggattggtaa aatgcagggc gaccaaaata tttgaatttg tctccctgac
     4981 cgctcggctt ccttcattaa ataattggca catcttcgtc caatcagtct caccggcaaa
     5041 tcacctgact gggtccccaa taattacgat ttaaattagt agcccgccta atgagcgggc
     5101 ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagcattc
     5221 agcattttcg tgtggcgctg attccgtttt ttgcggcgtt ttgcctgccg gtgtttgcgc
     5281 atccggaaac cctggtgaaa gtgaaagatg cggaagatca actgggtgcg cgcgtgggct
     5341 atattgaact ggatctgaac agcggcaaaa ttctggaatc ttttcgtccg gaagaacgtt
     5401 ttccgatgat gagcaccttt aaagtgctgc tgtgcggtgc ggttctgagc cgtgtggatg
     5461 cgggccagga acaactgggc cgtcgtattc attatagcca gaacgatctg gtggaatata
     5521 gcccggtgac cgaaaaacat ctgaccgatg gcatgaccgt gcgtgaactg tgcagcgcgg
     5581 cgattaccat gagcgataac accgcggcga acctgctgct gacgaccatt ggcggtccga
     5641 aagaactgac cgcgtttctg cataacatgg gcgatcatgt gacccgtctg gatcgttggg
     5701 aaccggaact gaacgaagcg attccgaacg atgaacgtga taccaccatg ccggcagcaa
     5761 tggcgaccac cctgcgtaaa ctgctgacgg gtgagctgct gaccctggca agccgccagc
     5821 aactgattga ttggatggaa gcggataaag tggcgggtcc gctgctgcgt agcgcgctgc
     5881 cggctggctg gtttattgcg gataaaagcg gtgcgggcga acgtggcagc cgtggcatta
     5941 ttgcggcgct gggcccggat ggtaaaccga gccgtattgt ggtgatttat accaccggca
     6001 gccaggcgac gatggatgaa cgtaaccgtc agattgcgga aattggcgcg agcctgatta
     6061 aacattggta aaccgataca attaaaggct ccttttggag cctttttttt tggacgaccc
     6121 ttgtcctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat
     6181 ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg
     6241 tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt
     6301 tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga
     6361 ggctggccgt aggccggccc taccggcgcg gcagcgttac ccgtgtcggc ggctccaacg
     6421 gctcgccatc gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc
     6481 gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg
     6541 gctggctggg cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg
     6601 tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa
     6661 ccaccacggc ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca
     6721 cgcggtcatt gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga
     6781 tccagcgctc ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga
     6841 tgagcttgga aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca
     6901 cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct
     6961 catgcgcttt gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga
     7021 tttctctgga ctgcgtggcc atgcttatct ccatgcggta ggggtgccgc acggttgcgg
     7081 caccatgcgc aatcagctgc aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa
     7141 agtggcagtc aattacagat tttctttaac ctacgcaatg agctattgcg gggggtgccg
     7201 caatgagctg ttgcgtaccc ccctttttta agttgttgat ttttaagtct ttcgcatttc
     7261 gccctatatc tagttctttg gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc
     7321 ttcggcgcgg ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg
     7381 cggccttcgg ccttgcccaa ggtggcgctg cccccttgga acccccgcac tcgccgccgt
     7441 gaggctcggg gggcaggcgg gcgggcttcg cccttcgact gcccccactc gcataggctt
     7501 gggtcgttcc aggcgcgtca aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc
     7561 gccttccact tggtgtccaa ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt
     7621 ccccagagaa aattaaaaaa attgatgggg caaggccgca ggccgcgcag ttggagccgg
     7681 tgggtatgtg gtcgaaggct gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc
     7741 aggcgcagcc tgtccatcag cttgtccagc agggttgtcc acgggccgag cgaagcgagc
     7801 cagccggtgg ccgctcgcgg ccatcgtcca catatccacg ggctggcaag ggagcgcagc
     7861 gaccgcgcag ggcgaagccc ggagagcaag cccgtagggg gaggcgatct cgagagcttg
     7921 catactcgcc agaaagatcc agaaaaactc gttgactgaa ccgattgtaa acgatcaggt
     7981 tcagattgac tcttattaca acgcatcgag tgaattgtca ggtgatcttt acggatatta
     8041 tcaaatcgat gaacaccgtt acggcattat tatccttgat gtgatggggc acggaatttc
     8101 atctgcattg attacgatgt ctcttcatcc tttgttccag cgccagatca cgcaaggtct
     8161 cagccccgtt aaagttatga aggaattgga ccggcacctt cacagcttgt ttcaaaatga
     8221 tgaggaagca agacattatt gcactgctat ttatctcgag attgatatag cacggcaaag
     8281 aattgattat gtcaacgcag ggcatccgcc ggcactttgg caggacgata gcggaactca
     8341 acatctgctt cacgcgacct cgcctccgat cgggatgttt gaagacttag agtttcagtc
     8401 aagcagcctt tcatacacgg aggacggaag attgcttctg tatacggatg gcgtgatgga
     8461 tcctacggct tcatgttatt tatttgattt actaaaggat catcctgatt cgccaatcgc
     8521 tgacctgaag gagaaaatcc ttacatcact gcagcatcaa aaggaagctc atcataagag
     8581 tgatgacgaa tgttttatat tagttgatgt aaaataaaat gatccatgag acataacaat
     8641 gatttgttac atctcatgga tttttttgtt
//